Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,794	T→C	A329A (GCA→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,794	0	T	C	97.6%	261.7 / ‑4.4	85	A329A (GCA→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (38/44);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAG‑ATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTA  >  NC_000913/64554‑65034                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    tGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGCGCTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCCTCGCTCGTTCGTGGAAAAATGGCATTATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCCATCTTTCATGTTATAATTCGCCAgagccg                                                                                                                                                                                                                                           >  2:887563/1‑246 (MQ=255)    ccGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGCGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGtt                                                                                                                                                                                                                                        >  1:228135/1‑251 (MQ=255)             cATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCgg                                                                                                                                                                                                                               <  1:826186/251‑1 (MQ=255)                     aGCCGCCATGGCTGTGGTGTTGCGGCACT‑ATGCAGAGATTAGGCGAGACATAACCAGCGCTGTGCATTCGCGGAAAAAAGAG‑ATGACCAAACGCCGCCACCGATTCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGGCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTGATGGAAAAT‑CTGCGTCACCTACTCGCAAGCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAc                                                                                                                                                                                                                         <  2:779621/249‑1 (MQ=255)                              ggCTGTCGTGTGGCGGC‑CTGTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGGGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAtt                                                                                                                                                                                                             <  2:817406/251‑1 (MQ=255)                               gCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAttt                                                                                                                                                                                                            <  1:492444/251‑1 (MQ=255)                               gCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAttt                                                                                                                                                                                                            <  1:92365/251‑1 (MQ=255)                                  ggCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCCACGATCCCCCGTGGCGGGCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTc                                                                                                                                                                                                           <  1:520436/249‑1 (MQ=255)                                   gggtgcgggcggaccgacGCCGAGAGTAGGCGCGACATAACCAGGGCGATGAATTCGAGGAAAAT‑GAGGATGACCAAATGCCGCCGCCGATCCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGTCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGt                                                                                                                                                                                                         <  2:353533/234‑1 (MQ=255)                                    cGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCggcc                                                                                                                                                                                                       >  1:824414/1‑248 (MQ=255)                                          gcggcACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGa                                                                                                                                                                                                 <  1:559907/251‑1 (MQ=255)                                           gggcACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGAt                                                                                                                                                                                                <  1:755995/250‑1 (MQ=255)                                                    cGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGT‑TCAATTTCGTCCATTCGATCCCACgg                                                                                                                                                                                         <  1:369568/250‑1 (MQ=255)                                                         agatctggcgagataTAACCAGCGCGCGGCATGCTAGGAAAATAGAG‑ATGACCAATCGGCGCCACCGATCCCACGTGGCGTTCTACCGGCAGGCCGTTCGCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCTTCACCAGCTCGCAATCCTTCAGGTTATAAGTCGCCAGCGCCGGTTGAACTTCGTCGATACGGCGG‑TCATTTTCGTCGATTCGATCCCACGGTTTATc                                                                                                                                                                                   <  2:482222/237‑1 (MQ=255)                                                         agaTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAATTTGTCCATTTGATCCCACggggtttc                                                                                                                                                                                   >  1:171737/1‑246 (MQ=255)                                                            gtAGGCGCGCTATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATTGCCAATCGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGGCGCTCGTTCGAGTAAAAATGGCATGAGTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAt                                                                                                                                                                                <  2:608198/250‑1 (MQ=255)                                                             tAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAt                                                                                                                                                                                <  1:247377/250‑1 (MQ=255)                                                              agggccaaCATAACCAGCGCGGTGAATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATa                                                                                                                                                                               <  2:695411/243‑1 (MQ=255)                                                               ggCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGGTATCGAt                                                                                                                                                                                >  1:885295/1‑248 (MQ=255)                                                                   cgACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGATTATCTTCGGCGCAACGGCGG‑TCAAATTCGTCCATTCGATCCCCCGGGTTATCGAttagtttt                                                                                                                                                                         >  1:399849/1‑244 (MQ=255)                                                                    gACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTc                                                                                                                                                                        <  1:261697/251‑1 (MQ=255)                                                                                         acgaggAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTATACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGTAAAAT‑CTGCGTCAACAGCTCGCAATCTTTAAGGTTATAAGACGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGG‑TCCATTTCTTCCCTTCTATCCCACGGGTGCTTGATAGATTTTCTTTCGCCTCATaaatcccg                                                                                                                                                     >  2:775717/5‑242 (MQ=255)                                                                                          cgaggAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTTTCGGTAGATTTTTCTTCGCCTAATAACTCCTGAGc                                                                                                                                                  >  1:361865/4‑251 (MQ=255)                                                                                           gaggAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑GCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAATTTCCTTCGCCTAATAACTTCTGAGCg                                                                                                                                                 >  1:313509/3‑251 (MQ=255)                                                                                               aaaaTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcgg                                                                                                                                             <  1:770950/251‑3 (MQ=255)                                                                                                aaaTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcggt                                                                                                                                            <  1:684579/251‑4 (MQ=255)                                                                                                aaaTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGGCTACCGGCAGGCAGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAAGCTTTCAGGTTATAATTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCAGTCGAGCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcggt                                                                                                                                            <  1:956647/251‑4 (MQ=255)                                                                                                  aTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTTTGGAAAAT‑CTGCGTCACAAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAACAGGCGG‑TCAATTTTGCCAATTCGGTCCACCGGGTTTTCGGTAGATTTTCCTTCGCCTCATaaatcctgtgtgaaggggt                                                                                                                                          >  1:416747/1‑233 (MQ=255)                                                                                                     gag‑aTGACCAATCGTCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCTGTTCACCGTCGCTCTTTCGAGTAAAAATGGCATGATTTCAGTTTTATGTAAAAA‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTGCGTCCATTCGATCCCACGGGTTAGCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCa                                                                                                                                       <  1:618466/251‑1 (MQ=255)                                                                                                       g‑aTGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGGCCAATTT‑GTTCATTCGATCCCCCGGGTGTTCGATAGATTTTCCTTCCCCTAATAACTCCTggaccacggtttccacc                                                                                                                                     >  2:805450/1‑235 (MQ=255)                                                                                                          tGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCCCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCCGTTTTATGGAAAAT‑CTGCGTCACCAGCTAGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTTGATCCCACGGGTTATCGATAGATTTTCCTTTCCCTAATAACTcccgaacgccgggttccagcgg                                                                                                                                   >  1:97478/1‑231 (MQ=255)                                                                                                              cAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGACACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGCAACGGCGG‑ACAATTTCGTCCTTTTGATCCCCCGGGCTATCGATAGATTTTCCTTCCCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGa‑a                                                                                                                               >  1:898768/1‑250 (MQ=255)                                                                                                                                ccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATTATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAAc                                                                                                            <  1:513782/249‑1 (MQ=255)                                                                                                                                 cccgttgctgtctacaggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTACGGAAAAT‑CTGCGGCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTGCCAGCGAGA‑ATGAAGAGAAATTCCAGAAcg                                                                                                           <  2:358334/235‑1 (MQ=255)                                                                                                                                      ggcGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑t                                                                                                     <  2:800480/249‑1 (MQ=255)                                                                                                                                          gtctaCCGGCAGTCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTAGCTTCGGCGAAACGGCGG‑TCAATTTCATCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCa                                                                                                 <  1:793469/247‑1 (MQ=255)                                                                                                                                              aCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGACCAATTT‑GTCCATTCGATCCCACGGGTTTTTGAAAGATTTTTCCTCGCCCAATAAATCCTTAGCGACGGTGTCCCGCGAGA‑ATGAAGAGAAATTCCAGAAACCGGA‑TTTCAgcgc                                                                                             >  2:746808/1‑251 (MQ=255)                                                                                                                                              aCCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTGCAg                                                                                                <  2:937240/248‑1 (MQ=255)                                                                                                                                                   aaGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGGTTCCACGGGTTTTCGCAAGATTTTCCTTTGCCTAATAACTCCTGAGCGACGGATTCCAGCGAGA‑ATGAAAAGAAATTCCAGAAAGCGGG‑TTTCAGCGcccca                                                                                         >  2:228499/2‑247 (MQ=255)                                                                                                                                                    aGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCCATTTCGTCCATTCGATCCCACGGGTTTTCGAGAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGT‑TTTCCGCGCCTCGAt                                                                                       >  1:789825/1‑251 (MQ=255)                                                                                                                                                    aGGCCGTTCAACGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGAAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTTGTCCATTCAATCACACGGGGTTTAGATAGCTTATCCTATGCCTAATTACTCCCTCGCGACGGCTTCCAGCGAGG‑ATGTAGAGAAATTACAAAAAGGGGA‑TTTCAGCGCCtagcg                                                                                       >  2:618221/1‑247 (MQ=255)                                                                                                                                                     ggCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCTCCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCTGA‑TTTCAGCGCCTCGATa                                                                                      <  2:95767/251‑1 (MQ=255)                                                                                                                                                      gCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAc                                                                                     <  1:451963/251‑1 (MQ=255)                                                                                                                                                       ccGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAcc                                                                                    <  1:13467/251‑1 (MQ=255)                                                                                                                                                        cggttaCCGTCGCTCGTTCGAGTAAAAAAGGCTGGTTTTCAGTGTTATGGAAAAT‑CTGCGTCCCCAGCTCGCCATCTTTCAGGTTATAAGTCGACAGCGCAGGTGTATCTTCGGCGAACCGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTGATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGTGGA‑TTTCAGCGCCTCGATActg                                                                                   <  2:514980/246‑3 (MQ=255)                                                                                                                                                        cGTTCACCGTCGCTCTTTCGAGTAAAAAGGGCATGATGGCAGGTTTATGGTAGAT‑CTGCGTCACCATCTCGCAATCTTTCAGGTTATAATGCGCCAGCACAGGTTTATCTTCGTCGAAACGGCGG‑TCAAGTTCGTCCATTCGATCCGTCGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGATCGCGGA‑TTTCTGCGCCTCGAAACCg                                                                                   <  2:315376/251‑1 (MQ=255)                                                                                                                                                        cGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGACGGGAAATTCCAGAACGCGGC‑TTTCAGCGCCTCGATACCg                                                                                   >  2:339578/1‑251 (MQ=255)                                                                                                                                                             aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGAt                                                                              <  1:62600/251‑1 (MQ=255)                                                                                                                                                                 tcgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCt                                                                                                                                                                <  2:317536/162‑1 (MQ=255)                                                                                                                                                                 tcgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGAGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCt                                                                                                                                                                >  1:317536/6‑167 (MQ=255)                                                                                                                                                                  cgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCTACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAAtt                                                                          >  1:183517/5‑250 (MQ=255)                                                                                                                                                                          cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGGGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAACGTCGATAATTAACCGACCt                                                                 >  2:198777/1‑251 (MQ=255)                                                                                                                                                                                 aaaTGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCCCGGGTTATAGATAAATTTTCCTTCGCCTAATCACTCCTGAGCGACGGGTTCCAGCGGAA‑ATGAAAAGAAATTCCCGAACCCGGA‑TTTAAGCTCCCCGGTACCGTCGGTAATTAGCCGCCCttttacat                                                          >  2:807709/1‑246 (MQ=255)                                                                                                                                                                                 aaaTGGCATGATTTCAGTTTTATGGAAAAT‑CTGAGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCGATTTCGTCCATTCGGTCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGGGACGGTTTCAAGCGAGG‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGGTAATTAGCCGACCTTTAGccg                                                          >  1:278791/1‑250 (MQ=255)                                                                                                                                                                                 aaaTGGCATGATATCAGTTTTATGGAAAAG‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCt                                                          <  1:74351/251‑1 (MQ=255)                                                                                                                                                                                     agaaTGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TTACTTTCGTCCCTTCGCACCCCCGGGTTCTCGAAAGATATTCCTTCGCCTAATAACATCTGAGCGACGGTTTCCCACGAGA‑ATGAAAACAAAATACAGACCACGGG‑TTTCAGCTCCCCGATAACGTCAATAATTatacacactttagacacgg                                                       >  2:820941/4‑232 (MQ=255)                                                                                                                                                                                      gCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGc                                                      >  1:478131/1‑250 (MQ=255)                                                                                                                                                                                        aTGATCTCAGTTTTATGGAA‑ATGCCGCGTCACTATCTCTCAATCTTTCAGTTTATAAGCTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTTGGCCATTCGATCCCTCGGGTTTACGATAGATTTGTCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGGCGTTAATTAGCCGACCTTTAGCCTGGTCaat                                                   <  2:702146/251‑2 (MQ=255)                                                                                                                                                                                             ttCAGTTTTATGGAAAAA‑CTGCGTCACCAACTCGCAAGATTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGT‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAGAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCACGATACCGTCGATAATTAGCCGACCTTTAGCTTGGGCAAAAAAg                                               <  2:850288/250‑1 (MQ=255)                                                                                                                                                                                                aGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGCCGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAAACGc                                           >  1:508925/1‑251 (MQ=255)                                                                                                                                                                                                       atggaaaagcTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCAGTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGttt                                      <  2:694179/241‑1 (MQ=255)                                                                                                                                                                                                        ggAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACTCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTa                                   <  1:989023/251‑1 (MQ=255)                                                                                                                                                                                                        ggAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTa                                   <  1:808272/251‑1 (MQ=255)                                                                                                                                                                                                            aaT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGCCGGTTTCCAGCGAGA‑ATGACGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACACTCGGAAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACTCCGTTTTTAAAGc                               >  2:809482/2‑251 (MQ=255)                                                                                                                                                                                                            aaT‑CTGCGTCACCAGCTCGCAATCTTTCAGGCTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGc                               >  1:489983/2‑251 (MQ=255)                                                                                                                                                                                                                cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGG‑TCAATTTTGTCCATTCGATCCCACGGGTTTTCTATAGATTTTCCTTCGCCTAATAACTCCTGCGCGCCGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACACGGA‑TTTCTGCGCCCCGATACCGGCGTGAATTAGACGGCCTGTAACCTGGGCAAAAAAGaagcgtgttttatagcccg                            >  2:194925/1‑233 (MQ=255)                                                                                                                                                                                                                  gCGTCACCAGCTCGGAAGCTTTCATGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCAGTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                            <  2:857565/249‑1 (MQ=255)                                                                                                                                                                                                                  gCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGc                          <  1:968025/251‑1 (MQ=255)                                                                                                                                                                                                                  gCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTAGCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGTA‑TTTCAGCTCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAA‑GACGCCGTTTTTAAAGCCg                             <  2:704933/247‑1 (MQ=255)                                                                                                                                                                                                                  gCGTCACCAGCTCGCAATCGTTCAGGTGATAAGTCGTCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                            <  1:543863/249‑1 (MQ=255)                                                                                                                                                                                                                   cGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCt                         <  1:728905/251‑1 (MQ=255)                                                                                                                                                                                                                    gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCATCGCAGGTTTATCTTCGGCTAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAAGTAGCTGACCTTTATCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTc                        <  2:399849/251‑1 (MQ=255)                                                                                                                                                                                                                    gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTc                        <  1:110660/251‑1 (MQ=255)                                                                                                                                                                                                                    gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAACTTTTCCTTCGCCTAATAACTCCTTAGCGACGGTTTCCAGCGGGA‑CTGGAGAGAAATTCCAAAACCCGGAATTTC‑GCGCCTCGGTACCGTCGATAATTAGCAGACCTCTAGCCTGGACAAAAAAAACACCGCTTTTAAAGCCgatccc                        >  2:848132/1‑246 (MQ=255)                                                                                                                                                                                                                     tCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGGTTCCAGCGAGA‑ATGAAGAGAAAATCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGTTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGATcg                       >  1:994266/1‑251 (MQ=255)                                                                                                                                                                                                                       aCCAGCTGGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑ACCATTTCGTCCATTCGATCCCAAGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGAGACGGTTTCCAGCGCGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAACGCCTCGCTACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTacaacactgctcgcg                     >  2:429466/1‑237 (MQ=255)                                                                                                                                                                                                                         cAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGCTTTTCCTTCGCCTAATAAATCCTGTGCGACGGTTTCCAGCGAGG‑ATGAAGAGAAATTCCCAAACGCGGA‑TTTTAGCACCTCGATACCGTCGAGAATTAACCGACCTTTAGCCCGGGCAAAAAAGACGACGTTTTTAAAACCggcacgccccc                   >  2:128705/1‑241 (MQ=255)                                                                                                                                                                                                                              cGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCCATTTCGGCCATTCGATCCCACGGGTTATCGGTAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTTTCCTGCG‑GACATGCAGAAGAATTCCCGAACGCGGA‑TTTCACCGCCACGCTACCGTCGTAAATTAGCCGACCCTTACCCTGGGCAAAAAAGACCCCGTTTTTAAGACCGTGCTcgccccccccc              >  2:556249/1‑243 (MQ=255)                                                                                                                                                                                                                               gCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGCAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATAGATCAATGTTCCTTCGCCTACTAACTCCTGCGCGGCGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAACGTCGATAATTAGCCGACCTATAACCTGGGCAAAAAAGACGCCGTTGTTAAAGCCGTGCTAGCGCCACTccc             >  2:324011/1‑250 (MQ=255)                                                                                                                                                                                                                                cAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAACCGACCTTTAGCCTGGGCAAAAAAGACGCCGTATTTAAAGCCGTGCTCGCGCCACTcccg            >  2:660940/1‑249 (MQ=255)                                                                                                                                                                                                                                 aaTCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGc           >  1:635403/1‑251 (MQ=255)                                                                                                                                                                                                                                  atccttCAGGTTATAAGTGGCCAACGCCAGTTTATCTTCCGCGAAACGGCTG‑TCAATTTCGTCCAGTCGTGCCCCCGGGTTATCGATAGATTATCCGTCGCCTAATATCTCCTGAGCGACTGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGATCGCGTA‑TTCCAGCGCCTCGAGACCTTCGATAATTAGCCGACCTTTACCCTGGGCAGAAAAGACGCCGTTTTTAAAGCCGTGATCGCGCCACTCCAGCt          <  2:43656/247‑1 (MQ=255)                                                                                                                                                                                                                                   tCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCAAGGGTTATCGGTAGATTTTCCTTCGCCTAATAACTCCTGGGCGGCGGGTTCCAGCGAGT‑ATGTAGAAAAATTTAAGAACGCGGG‑TTTCAGCGCCCCGATACCGTCGATAATTAGCCGACCTTTTGCCTGGGCAAAAAAGGAGCCGGTTTTAAAACCGTGCTTgcgcacctccacctc         >  2:104840/1‑240 (MQ=255)                                                                                                                                                                                                                                      ttCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCCCGCGCCACTCCAGCTca        <  2:419660/249‑2 (MQ=255)                                                                                                                                                                                                                                          ggTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgtta  <  1:626451/251‑8 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAG‑ATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTA  >  NC_000913/64554‑65034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑