Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 72,731 A→G M66T (ATG→ACG)  thiQ ← thiamine ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,7310AG93.7% 413.8 / 10.1 142M66T (ATG→ACG) thiQthiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base G (60/73);  total (64/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 142 total aligned reads displayed.

ACGCGCTAACGCCACTCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACA  >  NC_000913/72502‑72970
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
acgctcgaaCGCCCCGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgt                                                                                                                                                                                                                            <  2:580471/244‑3 (MQ=255)
aCGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgt                                                                                                                                                                                                                            <  1:841658/251‑3 (MQ=255)
 cgcgCTAACGCCCCGCGC‑GTGCGCTGCCCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgtc                                                                                                                                                                                                                           <  2:440351/251‑4 (MQ=255)
 cgcgCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTAGTGTGATCAACGccgtc                                                                                                                                                                                                                           <  2:617957/251‑4 (MQ=255)
  gcgcTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCg                                                                                                                                                                                                                          >  1:198050/1‑251 (MQ=255)
       aaCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGt                                                                                                                                                                                                                     >  1:378071/1‑251 (MQ=255)
       aaCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGt                                                                                                                                                                                                                     <  1:634120/251‑1 (MQ=255)
        aCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTc                                                                                                                                                                                                                    <  1:953007/251‑1 (MQ=255)
              cgcgctgt‑cgcggACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGaa                                                                                                                                                                                                              <  2:842988/249‑1 (MQ=255)
                gccctggcgcttACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTGAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAAc                                                                                                                                                                                                             <  2:725541/244‑1 (MQ=255)
                 gctgt‑cgctgACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCg                                                                                                                                                                                                           >  1:646560/1‑251 (MQ=255)
                    gt‑cgctgACCGCCGGAAAGTTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTTCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCgct                                                                                                                                                                                                         <  1:588771/250‑1 (MQ=255)
                       cgctgACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCgctggc                                                                                                                                                                                                      <  1:543253/251‑1 (MQ=255)
                            aCCGCCGGCAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCg                                                                                                                                                                                                 >  1:822365/1‑251 (MQ=255)
                                  ggaacgccgcccagtgaaCCGCGCCCTTAAATTATCAATCCCCATCGGGCGGGCGATAGCGTGCAGTTTCTCCTGCTGGGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGGCAGCGGACGGCGTGACGGCGGGGTCGTGGTGTGATCAACGCCGTCGATAGGCAGCGAACCGCTGGCTGGCGTCAg                                                                                                                                                                                             <  2:436913/233‑1 (MQ=255)
                                         tCGCCAGGTAACCGCGCCATTCAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGAACGTCAGGTGACTGAACAGGGTGTTTTCCTGAAACCGCATCGACCCCGGACTGCGTGACGGCGGCGTCGTGGTGTGTTCCACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCggg                                                                                                                                                                                    >  2:505817/1‑250 (MQ=255)
                                          cGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCg                                                                                                                                                                                   <  1:210217/251‑1 (MQ=255)
                                          cGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCGGGCGGGGGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAACCAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGAGCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCg                                                                                                                                                                                   <  2:240590/251‑1 (MQ=255)
                                           gccaggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCg                                                                                                                                                                                   >  1:986626/5‑250 (MQ=255)
                                           gCCCGGTAACAGCGCCAATAAATTATCAATCCCCATCTGGCGGTCGATAGCGTGCATTTTCCCCTGCTGTGCCGCGTTCAGTTTCAAGCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGACCTACGCCATCGATAGTCAGCGAACCCCTGGCTGGCGTCAGAAAACCGGCGa                                                                                                                                                                                  <  1:510127/251‑1 (MQ=255)
                                            ccatgtaccctcgccaatcaaTTATCAATCCCCATCTGGCGTGCGATAGCGTTCAATGTCGCCTGCTGTGCCGCGTTCAGTTTCAATCTCGGATTGATCCCCAGCCCGATGGTCTGCGCGACCGTCAGGTGACTGCACAGGTTGGTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGGCGAGAGGCAGCGACCCGCTGGCTGGCGTCAGAAATCCGGCGAt                                                                                                                                                                                 <  2:778446/232‑1 (MQ=255)
                                            ccaggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATCGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGAt                                                                                                                                                                                 >  2:697980/4‑251 (MQ=255)
                                            ccaggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGGCGTGGTGGGATCAACGCCGTCGATAGTCAGAGAACCGCTGGCTGGGGTAAGAAAACCGGCGac                                                                                                                                                                                 >  2:724056/4‑250 (MQ=255)
                                              aggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATc                                                                                                                                                                                >  1:896566/2‑250 (MQ=255)
                                              aggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCa                                                                                                                                                                               <  1:63614/250‑1 (MQ=255)
                                                 tAACCGCGACATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAAACGGCGATCaagt                                                                                                                                                                            >  2:489696/1‑249 (MQ=255)
                                                  aaCCGAGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCaagtt                                                                                                                                                                           >  1:195512/1‑248 (MQ=255)
                                                     cgcgCCATTAAAGTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGAGTCAGAAAACCGGCGATCAAGTTcag                                                                                                                                                                        <  1:953926/251‑1 (MQ=255)
                                                      gcgcCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTcagc                                                                                                                                                                       <  1:930543/251‑1 (MQ=255)
                                                          cATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAggg                                                                                                                                                                   >  1:788689/1‑251 (MQ=255)
                                                             tAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTa                                                                                                                                                                 <  1:544557/250‑1 (MQ=255)
                                                             tAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTAAGCAggggac                                                                                                                                                                >  1:530863/1‑248 (MQ=255)
                                                              aaaTTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTAc                                                                                                                                                                <  1:170253/250‑1 (MQ=255)
                                                                aTTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACttt                                                                                                                                                             <  2:207389/251‑1 (MQ=255)
                                                                aTTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACttt                                                                                                                                                             <  1:242747/251‑1 (MQ=255)
                                                                aTTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACttt                                                                                                                                                             <  1:892727/251‑1 (MQ=255)
                                                                aTTACCAATCCCCTTCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAATCCGGCGATCAAGTTCAGCAGGGTACttt                                                                                                                                                             <  2:755925/251‑1 (MQ=255)
                                                                 ttATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGCGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGAGGCAGCAAACCGGCGGTCACGTTCAGCAGGGTACtttt                                                                                                                                                            >  2:570215/1‑251 (MQ=255)
                                                                   aTCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTAc                                                                                                                                                          >  1:741318/1‑251 (MQ=255)
                                                                   aTCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGCAAACCGGCGATCAAATTCAGCAGGGTACttt                                                                                                                                                             >  1:374772/1‑248 (MQ=255)
                                                                   aTCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCGGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACttt                                                                                                                                                             <  2:374772/248‑1 (MQ=255)
                                                                    tCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTAcc                                                                                                                                                         >  1:991509/1‑251 (MQ=255)
                                                                    ccAATCCCCATCGTGCGGGCGATAGCGTGCATTTTCTCCGGCTGTGCCGCGTGCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCGGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACgttttcc                                                                                                                                                         <  2:858061/250‑4 (MQ=255)
                                                                     cAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTAcc                                                                                                                                                         <  2:222964/250‑1 (MQ=255)
                                                                     cAATCCCCATCTCGCGGTCGATAGCGTGCATTTTCGCCTGCTGTGCCGCGTTCAGGTTCAATCCCGGATTTAGCCCCAGCCCGATTGTCTTCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAATTTCAGCAGGGTACTTTTAcct                                                                                                                                                        <  2:977775/251‑2 (MQ=255)
                                                                       aTCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTAcctgc                                                                                                                                                      >  1:19153/1‑248 (MQ=255)
                                                                               cTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTg                                                                                                                                              >  1:311053/1‑251 (MQ=255)
                                                                                 ggcgggcgATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCtt                                                                                                                                               <  1:695279/248‑1 (MQ=255)
                                                                                    gggcgATAGCGTGCATTTCCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCg                                                                                                                                                                                                 <  1:35585/195‑1 (MQ=255)
                                                                                    gggcgATAGCGTGCATTTCCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGAAGGCGTGACGGCGGAATAGTTGTGTGATCTACGCCATCGATAGCCAGCGAACCGCTGGCTGGCg                                                                                                                                                                                                 >  2:35585/1‑195 (MQ=255)
                                                                                     ggcgATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGCTGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGCTCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGAGTCAGACAACCGGCAGTCAAGGTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCgg                                                                                                                                        >  2:93437/1‑250 (MQ=255)
                                                                                        gatatagTGCAATTTCTCCTGCTGTGCCGCGTTCAGTTCCAATCCCGTATTTAGCCCCAGCCCGATGGACTGCGCGACCTGCAGGTGACTGAACAGGTTGTTGGCCTGAAACAGCAGCGACACCGGACGGCGTGACGGCGGCGTCTTGGTGTGATCAACCCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCTGCGATCAAGTTCATCAGGTTACTTTAACCTGCGCCGCGTGGGCCGAgta                                                                                                                                     <  2:134599/245‑3 (MQ=255)
                                                                                                gCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCAcc                                                                                                                             >  1:134547/1‑251 (MQ=255)
                                                                                                gCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCAcc                                                                                                                             <  1:453700/251‑1 (MQ=255)
                                                                                                      tctccTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTcg                                                                                                                       >  1:702408/4‑251 (MQ=255)
                                                                                                         ccTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCTATGTTCTGCGCGACCGTCAGGTGACTGATCAGGTTGTTTTCCTTAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCCCCTGCTcgcct                                                                                                                    <  1:663798/251‑2 (MQ=255)
                                                                                                         ccTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTcgccg                                                                                                                    <  1:353024/251‑1 (MQ=255)
                                                                                                         ccTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTcgccg                                                                                                                    <  1:663796/251‑1 (MQ=255)
                                                                                                              ggtgccGCGTTCAGGTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTGTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTc                                                                                                               <  2:46414/247‑1 (MQ=255)
                                                                                                                  ccGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTAcc                                                                                                           <  1:703441/251‑1 (MQ=255)
                                                                                                                     cgTTCCGTTTCAAGCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGtt                                                                                                        <  2:210375/251‑1 (MQ=255)
                                                                                                                     cgTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGtt                                                                                                        <  1:50923/251‑1 (MQ=255)
                                                                                                                     cgTTCAGTTTCAATACCGGATTTAGCCCCAGCCCGATGTTCTGCGCGCCCGTCAGGTGGCTGAACAGGTTGTTTTCCTGAAACCGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGCTAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGGGGATCACCACCTGCTCGCCGCGTTCTACCGtt                                                                                                        >  2:133372/1‑251 (MQ=255)
                                                                                                                             ttCAATCCCGGATTTAGACCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACAGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGCGGATCGCCACCTGCTCGACGCGTTCTACCGTTAAGCTaa                                                                                                 >  2:48590/1‑250 (MQ=255)
                                                                                                                                      ggATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTCCTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCCACTGCTCGCCGCGTTCTCCCGTTAAGCTAAACCGCATCgg                                                                                       >  2:183990/1‑251 (MQ=255)
                                                                                                                                      ggATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCgg                                                                                       <  1:924442/251‑1 (MQ=255)
                                                                                                                                      ggATTTAGCCCCAGCCCGATGGTCTGCTCGACCGTCAGGGGACTGAACAGGTTGTTTTCCTGAAACAGCGTCGGCACCGGACGGCGTGACGGCGGCGTCGTGGTGGGATCAACTCCGTCGATAGTCAGCGAACCGCTGGCTGGGTTCAGAAAACCGGCGATCAAGTTCAGCAGGGTTCTTGTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCgg                                                                                       <  1:909621/251‑1 (MQ=255)
                                                                                                                                             gactcAGCCCGATGTCCTGCGCGCCCTTCAGGGGACTTATCAGGTTGTGTTCCTGAAACAGCCTCGTCACCGGACGGCGGTACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCTTTAAGCTAAAACGCATCGGCAAAtgt                                                                                <  2:533411/247‑2 (MQ=255)
                                                                                                                                             gCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAAtgt                                                                                >  1:591522/1‑250 (MQ=255)
                                                                                                                                                acAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGCTAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGGACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGGTAAGCTAAAACGCACAGGCAAAtgtggg                                                                             >  2:613640/2‑247 (MQ=255)
                                                                                                                                                    cccGATGTTCTGCGCGACCGTCAGGTGACTGCACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACTGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGAAAATGTTGGTaa                                                                          >  1:332812/1‑250 (MQ=255)
                                                                                                                                                       gATGTTCTGCGCGACCGTCAGGTGACTGAACAGGATGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCACGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAAAGCATCGGCAAATGTTGGTAAAGcc                                                                      >  2:819980/1‑251 (MQ=255)
                                                                                                                                                          gTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGGGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAgg                                                                   <  2:368970/251‑1 (MQ=255)
                                                                                                                                                            tcttcgCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCTACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGt                                                                  <  1:138613/245‑1 (MQ=255)
                                                                                                                                                              tgcgCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGat                                                               >  1:382230/4‑251 (MQ=255)
                                                                                                                                                                   gACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAATGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtc                                                            >  1:32640/1‑249 (MQ=255)
                                                                                                                                                                    aCCGTCAGGTGACTGAACAGGTTGGTTTCCTGAAACAGCATCGACAGCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtcagt                                                         <  1:8912/251‑1 (MQ=255)
                                                                                                                                                                      cGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTCCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTACAGCCAGGTGGTAtcagtc                                                        >  1:737438/1‑250 (MQ=255)
                                                                                                                                                                           ggggacTGAACAGGTAGTTTACCCGCAACAGCATCGTCACCAGACGGCGTGACGGCTGCGACGTGGTGCGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCTCCACCTGCTCGCCGCGTGCTACCTTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCCGtt                                                    <  2:281128/244‑1 (MQ=255)
                                                                                                                                                                            gtgacTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGtttt                                                  >  1:76012/5‑250 (MQ=255)
                                                                                                                                                                               acTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGCCGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCTCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAAAGTTGGTAAAGACAGGTGATATCAGACAGTTTTaaa                                               >  2:27655/2‑249 (MQ=255)
                                                                                                                                                                                  gAACAGGTTGTTTGCCTGAAACAGCATCGGCACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACAtct                                           <  2:378071/251‑3 (MQ=255)
                                                                                                                                                                                       ggTTGTTTTCCTGAAACGGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCCGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGCTCGCCACCTGCGCGCCGCGTTCTACCGTTAAGCTAAAACGCTTCGGCACATGTTGGTAAAGCCAGGTGATATCAGTCAGATTTAACATCTCGccc                                      >  2:18069/1‑251 (MQ=255)
                                                                                                                                                                                        gttgttTTCCTGAAACAGCATCGACACCGGACGGCGAGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTCCTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTCAGCTCAAACGCATCGGCAACAGTTGGTAACGCCCGGAGATATCTGTACGTTTTAACATCTCGcccc                                     >  2:662714/1‑251 (MQ=255)
                                                                                                                                                                                         ttgttTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCg                                    >  1:824724/1‑251 (MQ=255)
                                                                                                                                                                                           gttCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGt                                  <  1:833922/251‑1 (MQ=255)
                                                                                                                                                                                             tttcCTGAAACAGCATCGACACCGGAAGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAACAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTGAAGCCAGGACATATCAGTCAGTTTTAACATCTCCCCCCGGt                                  >  2:629233/3‑249 (MQ=255)
                                                                                                                                                                                              ttcCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCgctggct                                                                                                                                                                                                     <  1:560411/84‑1 (MQ=255)
                                                                                                                                                                                               tcCTGAAACAGCATCGACGCCGGACGGCGTTACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGt                               <  2:331123/250‑1 (MQ=255)
                                                                                                                                                                                                   gAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGttttt                           <  1:154573/250‑1 (MQ=255)
                                                                                                                                                                                                   gAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTc                          >  1:328612/1‑251 (MQ=255)
                                                                                                                                                                                                    aaaCAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTa                                                                                                                                                           <  1:300229/121‑1 (MQ=255)
                                                                                                                                                                                                              gACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCCCCTGCTCGCCGCGTTCTACCGTTACGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGAAAACAGTCAGATTTAACATCTCGCCCCGGTATGTTTTCattacaggaa                >  2:245499/1‑241 (MQ=255)
                                                                                                                                                                                                              gACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCCAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCCCGGTa                >  1:97242/1‑250 (MQ=255)
                                                                                                                                                                                                                 acCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAc             >  1:257442/1‑250 (MQ=255)
                                                                                                                                                                                                                    ggACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAcagca         <  1:90919/251‑1 (MQ=255)
                                                                                                                                                                                                                    ggACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAcagc          >  1:419630/1‑250 (MQ=255)
                                                                                                                                                                                                                       cGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGAAAACAGCAGa       >  1:845781/1‑250 (MQ=255)
                                                                                                                                                                                                                        ggCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGAGATATCAGTCAGTTTTAAACTCTCGCCCCGGTAGTTTTTCAATCACggataaaagcagca      >  2:181801/1‑238 (MQ=255)
                                                                                                                                                                                                                            tGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATCTCAGTCAGTTTTAACATCTCGCCCCGGAAGTTTTTCAATCACGGTAAACAGCAGAAcaca  >  1:556420/1‑250 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
ACGCGCTAACGCCACTCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACA  >  NC_000913/72502‑72970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑