Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 72,517 T→G R137R (CGA→CGC)  thiQ ← thiamine ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,5170TG95.7% 326.8 / ‑3.7 115R137R (CGA→CGC) thiQthiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (56/54);  total (57/58)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTC  >  NC_000913/72271‑72760
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
  tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACAACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACgcgctgt                                                                                                                                                                                                                                                >  1:487185/1‑251 (MQ=255)
        actcagtggtttgaCCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGa                                                                                                                                                                                                                                         <  2:34842/238‑1 (MQ=255)
         cTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGAc                                                                                                                                                                                                                                        >  1:210375/1‑251 (MQ=255)
           cATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAATTCGGCTGTTCGCGCACCCGACAACGCGCTAACGCCCCGCGCTGT‑CGCTGAccg                                                                                                                                                                                                                                      >  2:930543/1‑251 (MQ=255)
             ttGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGAccgcc                                                                                                                                                                                                                                    >  1:843879/1‑251 (MQ=255)
                  cttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTACCGCCACGCCCTGT‑CGCTGACCGCCGGaa                                                                                                                                                                                                                                >  2:881143/3‑250 (MQ=255)
                      cccTGCCAGGCGATTCGCCCGCCGGCGACCACCACCGAGCGCGTGGCGATCCGCTCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTc                                                                                                                                                                                                                           <  2:288321/251‑1 (MQ=255)
                           ccAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGTCCGCCGGAAAGCTCGccag                                                                                                                                                                                                                      <  1:640565/251‑3 (MQ=255)
                             atgCGATGCGCCCGTCGGCGACCACCACCGCGCGCGTGGCAATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCATCATCTCCTGACGTAACGCCGGATCTAGCGCAGAGAACGGTTCATCGAGCACTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTACCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGt                                                                                                                                                                                                                    >  2:603601/3‑251 (MQ=255)
                                 gctGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGAcc                                                                                                                                                                                                                                       <  2:710246/226‑1 (MQ=255)
                                 gATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGAcc                                                                                                                                                                                                                                       >  1:710246/1‑228 (MQ=255)
                                      gcCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATCACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCCGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGCGCAATACCATCGGCTGTTCGCACACCAGCCACCGCGCTAAAGCCACGCGCTGT‑CGCTGAACGCCGGaaatctaccacggtaaccaccgc                                                                                                                                                                                                           >  2:910172/1‑231 (MQ=255)
                                       cccgccggcgccccccacCGAGCGCGTGGCGTTCCGCGCGGCATCTGCCACACTGTTTGACCCCATCAATAACGTCATTTTTTGCGGCTGGCAGCTCGTGCGCACCAGCGTCAGCATCTCCTGACGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAGTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGC‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCa                                                                                                                                                                                                          <  2:862463/235‑1 (MQ=255)
                                          gTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGGGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCAGCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTa                                                                                                                                                                                                       <  2:670254/251‑1 (MQ=255)
                                          gTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGCCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt                                                                                                                                                                                                        >  1:983528/1‑250 (MQ=255)
                                           tCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGG‑CGCTGACCGCCGGAAAGCACGCCAGGAAACCGCGCCATTa                                                                                                                                                                                                       >  2:695279/1‑250 (MQ=255)
                                           ccggcgaccgccacCGAGCGCGTGGCGAGCCGCGCGGCATCTTCCACCCTGTGTGACACCATCAATAACGTCATTTTTGGCTGCTGGCAGCTCGTGCTCACCAGCTCCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTaa                                                                                                                                                                                                      <  2:352093/239‑1 (MQ=255)
                                           ccGGCTACTGCTACCGAGCGCGTGGTGTTCCGCGCCGCATCTTCCACGATGTGTGTCCCCACCAATATCGTCATTTTTTGCTGCTGGCATCTCGTGCTCACCAGCGTCAACAACTCCTGACGTAACGCCTGATCGTGCTCAGAGAACGGTTCATGGAGCAATAAAATCGGCTGGTCGCGTACCAGTGAACGCGCTTGTTTCTCTCGCTTT‑CGCTGACCTCCGGAAAGCTCGCCCGGTAACCGCGCCAtt                                                                                                                                                                                                        <  1:399201/248‑1 (MQ=255)
                                                gaccaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGAATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGCCCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTAt                                                                                                                                                                                                 >  1:778446/8‑251 (MQ=255)
                                                      cacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATcc                                                                                                                                                                                           <  1:817435/250‑1 (MQ=255)
                                                       acCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATccc                                                                                                                                                                                          >  1:207389/1‑251 (MQ=255)
                                                        ccGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATcccc                                                                                                                                                                                         <  1:402227/251‑1 (MQ=255)
                                                         cGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCACTAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTACCGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCCAACGCCACGAGCTGT‑CGCTGAACGCCGGTAAGCTCGCCAGGTAACCGCGCCATTAATATATCAAAccccc                                                                                                                                                                                        >  2:215156/1‑250 (MQ=255)
                                                            gcgcGTGGCGAATCGCGCGGCATCTGCCACACTGTTGGACACTATCATGAACGTCCTTTTTGGCTGCTGGCAGCTCGGGCTCACCAGCGTCAGCCTCCCCTGACGTAACGCCGGATCGTGCGCAGAGAACGGTTCACCGAGCAATAAAATCGGCTTTTCGCGCACCAGACAACGCGCTACCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCt                                                                                                                                                                                     <  2:375959/251‑1 (MQ=255)
                                                                 tGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcgg                                                                                                                                                                                >  1:170745/1‑251 (MQ=255)
                                                                  ggCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcggg                                                                                                                                                                               >  1:262726/1‑251 (MQ=255)
                                                                  ggCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcgg                                                                                                                                                                                <  2:262726/250‑1 (MQ=255)
                                                                               gcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAt                                                                                                                                                                  >  1:368970/1‑251 (MQ=255)
                                                                               gcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAt                                                                                                                                                                  >  1:580471/1‑251 (MQ=255)
                                                                               gcATCTTCCACACTGTGTGACACCATCAATAACGTCATGTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAt                                                                                                                                                                  <  2:948025/251‑1 (MQ=255)
                                                                                cATCTTCCACACTGTGTGACACCATCAATACCGTCATTTTTTGCTGCTGCCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGAGCAGAGCACGGTTCATCGAGAAATACAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTACATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAtt                                                                                                                                                                 >  1:134599/1‑251 (MQ=255)
                                                                                       cccacTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGGCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCct                                                                                                                                                          <  1:4434/247‑1 (MQ=255)
                                                                                        cctctgtttgaccccATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCTCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCTCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCTCACCAGCCAACGCGCTAACGCCACGCGCTTT‑CGCTGACCGCCGTAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctg                                                                                                                                                         <  1:46372/238‑1 (MQ=255)
                                                                                          acTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCGAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct                                                                                                                                                       <  2:372927/250‑1 (MQ=255)
                                                                                             gtgCGACACCATCAATAGCGTCATTTTTTGCGGCTGGCAGCTCGTGCTCACCAGCGTCATCATCTCCTGACGTAACGCCGGATCGAGCGCAGACAACGGTTCATCGAGCAATAAAAGCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCCGGGAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCctgctg                                                                                                                                                      <  2:520317/249‑1 (MQ=255)
                                                                                               gtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGtgcc                                                                                                                                                  <  1:898994/249‑4 (MQ=255)
                                                                                                tgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCcg                                                                                                                                                 >  1:617957/2‑251 (MQ=255)
                                                                                                ggACACCATCAATAACGGCATTTTTTGCTGCTGGCAGCCCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCACGCCAGGTAACCGCGCCATTAAATTATCAATCCCCACCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCcg                                                                                                                                                 <  1:583059/250‑1 (MQ=255)
                                                                                                    acCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGtt                                                                                                                                             <  1:500417/251‑1 (MQ=255)
                                                                                                         cAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGATCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACTCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGtt                                                                                                                                        <  1:467689/251‑1 (MQ=255)
                                                                                                           ataaactcatttttgtctgcctgCAGCCCGTGCTCACCTGCGTCAGCATCTCCGGACGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCCCCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTc                                                                                                                                      <  2:358514/229‑1 (MQ=255)
                                                                                                                    ttttttGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCCGAGAACGGTTCATCGCGCAATAAAATCGGCTGTTCGCGCACCAGCCCACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCCTTACATTATCAATCCCCCTCTGGCGGGCGTAAGCGTGCAATTTCTCCTGCTGTGCCGCGTTCCGTTTCAATCCCggg                                                                                                                             >  2:405773/1‑250 (MQ=255)
                                                                                                                       tttGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGCttt                                                                                                                          >  1:139372/1‑251 (MQ=255)
                                                                                                                             gctgGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGcc                                                                                                                      <  1:248287/249‑1 (MQ=255)
                                                                                                                                    gCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCCTCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCg                                                                                                              >  2:50923/1‑250 (MQ=255)
                                                                                                                                        gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCCGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGCAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCCTCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTACATCCCGGCTTTACCCCCATCCCGATGtt                                                                                                         >  2:114224/1‑251 (MQ=255)
                                                                                                                                        gTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGATGT‑CGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt                                                                                                         <  1:157188/251‑1 (MQ=255)
                                                                                                                                         tGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTc                                                                                                        >  1:302217/1‑251 (MQ=255)
                                                                                                                                          gCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCt                                                                                                       >  1:331123/1‑251 (MQ=255)
                                                                                                                                             ctccAGCTTCAGCATCACCTTACGTAACGCCGGATCGAGCGCAGATAACGGTTCAACCAGCAATAAAATCGGCTTGTCGCGCACCAGACAACCCGCGAACGCCACGCGCTGT‑GGCTGACCGCCGGAAAGCGCGCCAGGTAACCGCGCCATTAAATTATCAATCCTCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCTGATGTTCtg                                                                                                      <  2:501275/247‑1 (MQ=255)
                                                                                                                                             cACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGTAGTTCtgcg                                                                                                    >  2:154573/1‑249 (MQ=255)
                                                                                                                                             cACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCtgcg                                                                                                    <  1:944069/251‑3 (MQ=255)
                                                                                                                                                  gCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAAcc                                                                                                                                                                                                                >  2:293317/1‑138 (MQ=255)
                                                                                                                                                   cGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCg                                                                                              <  1:134390/251‑1 (MQ=255)
                                                                                                                                                    gTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGt                                                                                             >  1:147182/1‑251 (MQ=255)
                                                                                                                                                         cATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGACAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGCCCGTCAGGt                                                                                        >  2:543253/1‑251 (MQ=255)
                                                                                                                                                          aTCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCACGGATTTAGACCCAGCCCGATGTTCTGCGCGACCGTCAgggg                                                                                       >  1:281128/1‑249 (MQ=255)
                                                                                                                                                                    gTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAgg                                                                             <  1:627829/251‑1 (MQ=255)
                                                                                                                                                                    gTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAgg                                                                             >  1:391861/1‑251 (MQ=255)
                                                                                                                                                                    gTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGAGACTGAACAgg                                                                             >  1:599934/1‑251 (MQ=255)
                                                                                                                                                                     tAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGgt                                                                            >  1:46414/1‑251 (MQ=255)
                                                                                                                                                                         gCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAAGCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGgttgtt                                                                        <  1:918389/251‑1 (MQ=255)
                                                                                                                                                                               gcGAGCGCAGAGAACGGTTCACCGAGAAATAAAATCGGCTGTTCGCGCACCAGACAACGCTCTAACGCCACGCGCTGT‑CGCTGTCCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTg                                                                  <  2:798219/250‑1 (MQ=255)
                                                                                                                                                                                         agaACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATc                                                        >  2:19534/1‑251 (MQ=255)
                                                                                                                                                                                           cgcacttccgatataaaTAAAAGCGGCTGGTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTAGCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATGTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTGGTTTTCCTGAAACAGCATCGa                                                      <  2:394046/236‑1 (MQ=255)
                                                                                                                                                                                             cGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGaca                                                    <  1:900230/251‑1 (MQ=255)
                                                                                                                                                                                             cGGTTCATCGAGCAATAAAATCGGATGTTCGCGCACCAGACAACGCGCTAACGCCCCGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGCACGGGTTGTTTTACTGAAACAGCATCGacc                                                    >  2:712461/1‑250 (MQ=255)
                                                                                                                                                                                               gtgcATCGAGAAAAAAACTCGGCTTCTCGCGCACCACACACCGCGCTTACGCCACTCGCGGT‑CGCAGACCTCCGGAAAGCTCGCCGTGGAACCGCGCCATTAAATTATCAATCCCCATCTGGCTGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTAAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCTACCGTCATGTTACTGAACAGGTTGTTTTCCTGAAACATCATCGACAcc                                                  <  2:457535/248‑1 (MQ=255)
                                                                                                                                                                                               gTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGCAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGCTGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACAcc                                                  >  1:651348/1‑251 (MQ=255)
                                                                                                                                                                                                ttCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCAACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGAGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGATGGTTTCATGAAACAGCATCGACACCg                                                 >  1:865158/1‑251 (MQ=255)
                                                                                                                                                                                                 tCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCgg                                                >  1:242983/1‑251 (MQ=255)
                                                                                                                                                                                                  cATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCTGGTAACAGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCgg                                                >  1:671123/1‑250 (MQ=255)
                                                                                                                                                                                                  cATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACCCCgg                                                >  2:210217/1‑250 (MQ=255)
                                                                                                                                                                                                   aTCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGAc                                              <  1:733126/251‑1 (MQ=255)
                                                                                                                                                                                                   aTCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGAc                                              >  1:554357/1‑251 (MQ=255)
                                                                                                                                                                                                     cGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCAATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTATCCTGCAACAGCATCGACACCgggagg                                            >  2:663796/1‑247 (MQ=255)
                                                                                                                                                                                                     agAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGA‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCAATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGAGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCAAGCCCGATGTTCTTCGCGACCGTCAGGTGCCTGGACAGGTTGTTTTCCTGAAACAGCATCGAACCCGGACgg                                            >  2:663798/2‑251 (MQ=255)
                                                                                                                                                                                                        gCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGt                                         <  1:22540/251‑1 (MQ=255)
                                                                                                                                                                                                         cAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGACACAGCATCGa                                                      >  1:394046/1‑237 (MQ=255)
                                                                                                                                                                                                            tAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGGTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcg                                     >  1:328672/1‑251 (MQ=255)
                                                                                                                                                                                                             aaatccGGCTGTCCGCCCACCAGACACCGCGCTAACACCTCGCTCTGG‑CGCTGTCCGCCGGAAAGCTCGTCAGGTATCCGCGCAATTAAATGATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTACTCCGGCGGTGCCGCGTTCAGTTTCAATCCCGGATTTAGTCCCAGCCCGATGTTCGGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCTACGCCGGACGGCGTGAcgg                                    <  2:422089/246‑1 (MQ=255)
                                                                                                                                                                                                             aaaaTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCTCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcg                                     <  1:15569/250‑1 (MQ=255)
                                                                                                                                                                                                              aaaTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcggc                                   >  1:160748/1‑251 (MQ=255)
                                                                                                                                                                                                              aaaTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcggc                                   <  1:719161/251‑1 (MQ=255)
                                                                                                                                                                                                               aaTCGCCTGGTCGCGCCCCAGACAACGCGCTAACGCCCCGCGCTGT‑CGCTGACCGCCGGAAAGCGCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTTCATTTTCTCCTGCGGTGCCGCGTTCAGTGTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACTCCGGACGGCGTGAcggcg                                  <  2:628311/251‑1 (MQ=255)
                                                                                                                                                                                                                     cTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCACTCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcg                            >  1:754285/1‑247 (MQ=255)
                                                                                                                                                                                                                       gTTCGCGCACCAGACAACGCGCTAACGAAACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtg                          >  1:880136/1‑245 (MQ=255)
                                                                                                                                                                                                                         gcgcgCACCAGACAACGCGCTAACGCCACGCGCGGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAGCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggt                        <  2:53775/250‑9 (MQ=255)
                                                                                                                                                                                                                          cgcgCACCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggt                        <  1:339767/250‑9 (MQ=255)
                                                                                                                                                                                                                               aCCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACTGCGTGACggcggc                                >  2:656031/1‑237 (MQ=255)
                                                                                                                                                                                                                               aCCAGACAACGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggc                                <  1:656031/237‑1 (MQ=255)
                                                                                                                                                                                                                                    acaccgagcgaaCGCCACGCGCTGT‑CGCTGCCCGCCGGAAAGCCCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGc             <  2:56747/241‑1 (MQ=255)
                                                                                                                                                                                                                                       acgctcgaaCGCCCCGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgt          <  2:580471/244‑3 (MQ=255)
                                                                                                                                                                                                                                       aCGCGCTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgt          <  1:841658/251‑3 (MQ=255)
                                                                                                                                                                                                                                        cgcgCTAACGCCCCGCGC‑GTGCGCTGCCCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgtc         <  2:440351/251‑4 (MQ=255)
                                                                                                                                                                                                                                        cgcgCTAACGCCACTCGCTGT‑CGCTGACCGCCGCAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTTCATTTTCCCCTGCTGTTCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGGGTGTTCTaagacatc         >  2:833922/1‑244 (MQ=255)
                                                                                                                                                                                                                                         gcgcTAACGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCg        >  1:198050/1‑251 (MQ=255)
                                                                                                                                                                                                                                              aaCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGt   <  1:634120/251‑1 (MQ=255)
                                                                                                                                                                                                                                              aaCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGt   >  1:378071/1‑251 (MQ=255)
                                                                                                                                                                                                                                               aCGCCACGCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTc  <  1:953007/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
GCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGT‑CGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTC  >  NC_000913/72271‑72760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑