Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,755	G→A	I342I (ATC→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,755	0	G	A	97.5%	250.3 / ‑3.8	82	I342I (ATC→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (35/43);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAG‑ATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTA  >  NC_000913/64508‑65001                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          ggTACAGGCTTTTATAGGTCAGCACCAGTACCGAATCAGAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑c                                                                                                                                                                                                                                                       <  1:533195/251‑1 (MQ=255)          ttttATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCCCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCAcc                                                                                                                                                                                                                                              >  1:419660/1‑251 (MQ=255)                 gTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCCGCCGGGCGTTCACCCTCCCCCGGTCGAGTAAAAATTGCATGGTTTTAGTTTTTTGGAAAAT‑Cttctttacccgctagc                                                                                                                                                                                                                                       >  2:684579/1‑236 (MQ=255)                      gcaccagtacCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGGTCCCCGTCGCTCGTTCGAGGAAAACTGGCATGATTTCAGGTTTATGGAAAAT‑Ctcgctccccagcttcgaatct                                                                                                                                                                                                                                  >  2:13467/1‑231 (MQ=255)                      gcaccagtacCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGAACT‑TCGCCGCGATTAGGCGCGACATAACCAGCGCGGGGCATACGGGGAAAATAGAG‑ATGACCAAACACCGCCACCGATCCCCCGTGGCGGTATACCGGCAGGCCGTGCACCGTCGCTCGTTCGAGTAAAAAAGGCTTGGTTTTAGTTTTATGGAAAAT‑CTggctaccccggccgcaaact                                                                                                                                                                                                                                  >  2:825712/1‑232 (MQ=255)                        cacagtaccgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCttt                                                                                                                                                                                                                               <  2:534394/242‑1 (MQ=255)                        accagtacCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCttt                                                                                                                                                                                                                               <  1:840602/251‑1 (MQ=255)                           agtaccgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAg                                                                                                                                                                                                                            <  1:903952/245‑1 (MQ=255)                           agtaacgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGTG‑ATGACCAAACGCCGCCACCGATCCCCCGTAGCGGTCTACCCGCCGGCCGTTCCCCGTCGCTCGTTCGCGTAAAAAAGCCCAGATTTCAGATTTATGGAATAT‑CTG‑GTCACCCAGCCCGCaaccgttc                                                                                                                                                                                                                              >  1:667554/7‑243 (MQ=255)                            gtaccgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCCGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGGGCATACGAGGAAAATAGGG‑ATGCCCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCCCCGGCGCTCGTTCGAGGAAAAATGGGATGATATCAGTTTTATGGAAAAT‑CTTGGTCCCC‑AGTCCTCAATCTTTCAgg                                                                                                                                                                                                                           >  2:74351/6‑251 (MQ=255)                             taccgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGGTCACCGTCGCTCGTTCGAGTAAAAATAGCATGTTTTCAGTTTTATGGAAAAA‑CTGCGTCACC‑AGATCGCAAACTTTCAGGt                                                                                                                                                                                                                          >  2:930517/5‑251 (MQ=255)                              accgAATCATAAAGCCCTGGCCGGGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGt                                                                                                                                                                                                                          <  1:52860/247‑1 (MQ=255)                                 gAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGGTCACCCTCGCGTGGTAGTGTAAAAATGGCATGGTTTCAGTTTTATGGAAAAT‑CTGAGGTACC‑AGCTCGCAATCTTTTAGGttca                                                                                                                                                                                                                       >  2:676429/1‑248 (MQ=255)                                   aTCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAg                                                                                                                                                                                                                    <  1:811190/251‑1 (MQ=255)                                   aTCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAg                                                                                                                                                                                                                    <  1:454149/251‑1 (MQ=255)                                     cATAAAGCCCTGGCCGTGAATCCATCACGGAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGt                                                                                                                                                                                                                   <  2:534656/250‑1 (MQ=255)                                         aaGCCCTGGCCGGGAATCCATCACGGAGCCGCCAGGGCGGGCGTGTGCCGGCCCA‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGACACCGATTCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTTGCAATCTTTCAGGTTATAAGtcgtca                                                                                                                                                                                                              <  2:712413/251‑6 (MQ=255)                                            cccTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTAGCTCGTTCGAGTAAAAAAGGCCTGATTTCCGTTTTATGGGAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTTAGGGTATaaatcgacagcg                                                                                                                                                                                                           >  2:728905/1‑241 (MQ=255)                                               tGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGCGCTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCCTCGCTCGTTCGTGGAAAAATGGCATTATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCCATCTTTCATGTTATAATTCGCCAgagccg                                                                                                                                                                                                        >  2:887563/1‑246 (MQ=255)                                                  ccGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGCGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGtt                                                                                                                                                                                                     >  1:228135/1‑251 (MQ=255)                                                           cATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCgg                                                                                                                                                                                            <  1:826186/251‑1 (MQ=255)                                                                   aGCCGCCATGGCTGTGGTGTTGCGGCACT‑ATGCAGAGATTAGGCGAGACATAACCAGCGCTGTGCATTCGCGGAAAAAAGAG‑ATGACCAAACGCCGCCACCGATTCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGGCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTGATGGAAAAT‑CTGCGTCACC‑TACTCGCAAGCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAc                                                                                                                                                                                      <  2:779621/249‑1 (MQ=255)                                                                            ggCTGTCGTGTGGCGGC‑CTGTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGGGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAtt                                                                                                                                                                          <  2:817406/251‑1 (MQ=255)                                                                             gCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAttt                                                                                                                                                                         <  1:92365/251‑1 (MQ=255)                                                                             gCTGGCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAttt                                                                                                                                                                         <  1:492444/251‑1 (MQ=255)                                                                                ggCGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCCACGATCCCCCGTGGCGGGCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTc                                                                                                                                                                        <  1:520436/249‑1 (MQ=255)                                                                                 gggtgcgggcggaccgacGCCGAGAGTAGGCGCGACATAACCAGGGCGATGAATTCGAGGAAAAT‑GAGGATGACCAAATGCCGCCGCCGATCCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGTCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGt                                                                                                                                                                      <  2:353533/234‑1 (MQ=255)                                                                                  cGTGTGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCggcc                                                                                                                                                                    >  1:824414/1‑248 (MQ=255)                                                                                        gcggcACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGa                                                                                                                                                              <  1:559907/251‑1 (MQ=255)                                                                                         gggcACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGAt                                                                                                                                                             <  1:755995/250‑1 (MQ=255)                                                                                                  cGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGT‑TCAATTTCGTCCATTCGATCCCACgg                                                                                                                                                      <  1:369568/250‑1 (MQ=255)                                                                                                       agatctggcgagataTAACCAGCGCGCGGCATGCTAGGAAAATAGAG‑ATGACCAATCGGCGCCACCGATCCCACGTGGCGTTCTACCGGCAGGCCGTTCGCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCTTCACC‑AGCTCGCAATCCTTCAGGTTATAAGTCGCCAGCGCCGGTTGAACTTCGTCGATACGGCGG‑TCATTTTCGTCGATTCGATCCCACGGTTTATc                                                                                                                                                <  2:482222/237‑1 (MQ=255)                                                                                                       agaTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAAATTTGTCCATTTGATCCCACggggtttc                                                                                                                                                >  1:171737/1‑246 (MQ=255)                                                                                                          gtAGGCGCGCTATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATTGCCAATCGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGGCGCTCGTTCGAGTAAAAATGGCATGAGTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAt                                                                                                                                             <  2:608198/250‑1 (MQ=255)                                                                                                           tAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAt                                                                                                                                             <  1:247377/250‑1 (MQ=255)                                                                                                            agggccaaCATAACCAGCGCGGTGAATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATa                                                                                                                                            <  2:695411/243‑1 (MQ=255)                                                                                                            aGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCtt                                                                                                                                                                                                                                >  1:881385/1‑167 (MQ=255)                                                                                                            aGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCtt                                                                                                                                                                                                                                <  2:881385/167‑1 (MQ=255)                                                                                                             ggCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGGTATCGAt                                                                                                                                             >  1:885295/1‑248 (MQ=255)                                                                                                                 cgACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGATTATCTTCGGCGCAACGGCGG‑TCAAATTCGTCCATTCGATCCCCCGGGTTATCGAttagtttt                                                                                                                                      >  1:399849/1‑244 (MQ=255)                                                                                                                  gACATAACCAGCGCGGTGCATACGAGGAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTc                                                                                                                                     <  1:261697/251‑1 (MQ=255)                                                                                                                                       acgaggAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTATACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGTAAAAT‑CTGCGTCAAC‑AGCTCGCAATCTTTAAGGTTATAAGACGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGG‑TCCATTTCTTCCCTTCTATCCCACGGGTGCTTGATAGATTTTCTTTCGCCTCATaaatcccg                                                                                                                  >  2:775717/5‑242 (MQ=255)                                                                                                                                        cgaggAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTTTCGGTAGATTTTTCTTCGCCTAATAACTCCTGAGc                                                                                                               >  1:361865/4‑251 (MQ=255)                                                                                                                                         gaggAAAATAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑GCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAATTTCCTTCGCCTAATAACTTCTGAGCg                                                                                                              >  1:313509/3‑251 (MQ=255)                                                                                                                                             aaaaTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcgg                                                                                                          <  1:770950/251‑3 (MQ=255)                                                                                                                                              aaaTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcggt                                                                                                         <  1:684579/251‑4 (MQ=255)                                                                                                                                              aaaTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGGCTACCGGCAGGCAGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAAGCTTTCAGGTTATAATTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCAGTCGAGCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcggt                                                                                                         <  1:956647/251‑4 (MQ=255)                                                                                                                                                aTAGAG‑ATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTTTGGAAAAT‑CTGCGTCACA‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAACAGGCGG‑TCAATTTTGCCAATTCGGTCCACCGGGTTTTCGGTAGATTTTCCTTCGCCTCATaaatcctgtgtgaaggggt                                                                                                       >  1:416747/1‑233 (MQ=255)                                                                                                                                                   gag‑aTGACCAATCGTCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCTGTTCACCGTCGCTCTTTCGAGTAAAAATGGCATGATTTCAGTTTTATGTAAAAA‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTGCGTCCATTCGATCCCACGGGTTAGCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCa                                                                                                    <  1:618466/251‑1 (MQ=255)                                                                                                                                                     g‑aTGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGGCCAATTT‑GTTCATTCGATCCCCCGGGTGTTCGATAGATTTTCCTTCCCCTAATAACTCCTggaccacggtttccacc                                                                                                  >  2:805450/1‑235 (MQ=255)                                                                                                                                                        tGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCCCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCCGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTAGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTTGATCCCACGGGTTATCGATAGATTTTCCTTTCCCTAATAACTcccgaacgccgggttccagcgg                                                                                                >  1:97478/1‑231 (MQ=255)                                                                                                                                                            cAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGACACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGCAACGGCGG‑ACAATTTCGTCCTTTTGATCCCCCGGGCTATCGATAGATTTTCCTTCCCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGaa                                                                                             >  1:898768/1‑250 (MQ=255)                                                                                                                                                                              ccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATTATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAAc                                                                          <  1:513782/249‑1 (MQ=255)                                                                                                                                                                               cccgttgctgtctacaggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTACGGAAAAT‑CTGCGGCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTGCCAGCGAGAATGAAGAGAAATTCCAGAAcg                                                                         <  2:358334/235‑1 (MQ=255)                                                                                                                                                                                    ggcGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGAt                                                                    <  2:800480/249‑1 (MQ=255)                                                                                                                                                                                        gtctaCCGGCAGTCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTAGCTTCGGCGAAACGGCGG‑TCAATTTCATCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCa                                                                <  1:793469/247‑1 (MQ=255)                                                                                                                                                                                            aCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGACCAATTT‑GTCCATTCGATCCCACGGGTTTTTGAAAGATTTTTCCTCGCCCAATAAATCCTTAGCGACGGTGTCCCGCGAGAATGAAGAGAAATTCCAGAAACCGGATTTCAgcgc                                                            >  2:746808/1‑251 (MQ=255)                                                                                                                                                                                            aCCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTGCAg                                                               <  2:937240/248‑1 (MQ=255)                                                                                                                                                                                                 aaGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGGTTCCACGGGTTTTCGCAAGATTTTCCTTTGCCTAATAACTCCTGAGCGACGGATTCCAGCGAGAATGAAAAGAAATTCCAGAAAGCGGGTTTCAGCGcccca                                                        >  2:228499/2‑247 (MQ=255)                                                                                                                                                                                                  aGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCCATTTCGTCCATTCGATCCCACGGGTTTTCGAGAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGTTTTCCGCGCCTCGAt                                                      >  1:789825/1‑251 (MQ=255)                                                                                                                                                                                                  aGGCCGTTCAACGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGAAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTTGTCCATTCAATCACACGGGGTTTAGATAGCTTATCCTATGCCTAATTACTCCCTCGCGACGGCTTCCAGCGAGGATGTAGAGAAATTACAAAAAGGGGATTTCAGCGCCtagcg                                                      >  2:618221/1‑247 (MQ=255)                                                                                                                                                                                                   ggCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCTCC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCTGATTTCAGCGCCTCGATa                                                     <  2:95767/251‑1 (MQ=255)                                                                                                                                                                                                    gCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATAc                                                    <  1:451963/251‑1 (MQ=255)                                                                                                                                                                                                     ccGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATAcc                                                   <  1:13467/251‑1 (MQ=255)                                                                                                                                                                                                      cggttaCCGTCGCTCGTTCGAGTAAAAAAGGCTGGTTTTCAGTGTTATGGAAAAT‑CTGCGTCCCC‑AGCTCGCCATCTTTCAGGTTATAAGTCGACAGCGCAGGTGTATCTTCGGCGAACCGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTGATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGTGGATTTCAGCGCCTCGATActg                                                  <  2:514980/246‑3 (MQ=255)                                                                                                                                                                                                      cGTTCACCGTCGCTCTTTCGAGTAAAAAGGGCATGATGGCAGGTTTATGGTAGAT‑CTGCGTCACC‑ATCTCGCAATCTTTCAGGTTATAATGCGCCAGCACAGGTTTATCTTCGTCGAAACGGCGG‑TCAAGTTCGTCCATTCGATCCGTCGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGATCGCGGATTTCTGCGCCTCGAAACCg                                                  <  2:315376/251‑1 (MQ=255)                                                                                                                                                                                                      cGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGACGGGAAATTCCAGAACGCGGCTTTCAGCGCCTCGATACCg                                                  >  2:339578/1‑251 (MQ=255)                                                                                                                                                                                                           aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGAt                                             <  1:62600/251‑1 (MQ=255)                                                                                                                                                                                                               tcgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCt                                                                                                                             <  2:317536/162‑1 (MQ=255)                                                                                                                                                                                                               tcgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGAGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCt                                                                                                                             >  1:317536/6‑167 (MQ=255)                                                                                                                                                                                                                cgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCTACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAAtt                                         >  1:183517/5‑250 (MQ=255)                                                                                                                                                                                                                        cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGGGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATAACGTCGATAATTAACCGACCt                                >  2:198777/1‑251 (MQ=255)                                                                                                                                                                                                                               aaaTGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCCCGGGTTATAGATAAATTTTCCTTCGCCTAATCACTCCTGAGCGACGGGTTCCAGCGGAAATGAAAAGAAATTCCCGAACCCGGATTTAAGCTCCCCGGTACCGTCGGTAATTAGCCGCCCttttacat                         >  2:807709/1‑246 (MQ=255)                                                                                                                                                                                                                               aaaTGGCATGATTTCAGTTTTATGGAAAAT‑CTGAGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCGATTTCGTCCATTCGGTCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGGGACGGTTTCAAGCGAGGATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGGTAATTAGCCGACCTTTAGccg                         >  1:278791/1‑250 (MQ=255)                                                                                                                                                                                                                               aaaTGGCATGATATCAGTTTTATGGAAAAG‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCt                         <  1:74351/251‑1 (MQ=255)                                                                                                                                                                                                                                   agaaTGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TTACTTTCGTCCCTTCGCACCCCCGGGTTCTCGAAAGATATTCCTTCGCCTAATAACATCTGAGCGACGGTTTCCCACGAGAATGAAAACAAAATACAGACCACGGGTTTCAGCTCCCCGATAACGTCAATAATTatacacactttagacacgg                      >  2:820941/4‑232 (MQ=255)                                                                                                                                                                                                                                    gCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGc                     >  1:478131/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTGATCTCAGTTTTATGGAA‑ATGCCGCGTCACT‑ATCTCTCAATCTTTCAGTTTATAAGCTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTTGGCCATTCGATCCCTCGGGTTTACGATAGATTTGTCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGGCGTTAATTAGCCGACCTTTAGCCTGGTCaat                  <  2:702146/251‑2 (MQ=255)                                                                                                                                                                                                                                           ttCAGTTTTATGGAAAAA‑CTGCGTCACC‑AACTCGCAAGATTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGT‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAGAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCACGATACCGTCGATAATTAGCCGACCTTTAGCTTGGGCAAAAAAg              <  2:850288/250‑1 (MQ=255)                                                                                                                                                                                                                                              aGTTTTATGGAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGCCGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAAACGc          >  1:508925/1‑251 (MQ=255)                                                                                                                                                                                                                                                      ggAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACTCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTa  <  1:989023/251‑1 (MQ=255)                                                                                                                                                                                                                                                      ggAAAAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTa  <  1:808272/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          GGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACT‑TCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAG‑ATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACC‑AGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTA  >  NC_000913/64508‑65001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑