BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,569	T→C	I73I (ATT→ATC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,569	0	T	C	97.5%	240.3 / ‑3.6	82	I73I (ATT→ATC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (18/60); total (20/62)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTA‑TTGGCTG‑CT‑TGATGGGCGACTGGAT‑TTCTTTCTGGCTGGGTTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTT  >  NC_000913/71333‑71811

ccATTTGATAGGCACATTATGCATGCATTGCAGTAACTATTTATTACCCAATCTACCGTGTATTCAGTGATGGCGGTGGTGTTGGTGGCCGTTCTGGAGGCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGATGGTAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGTCTGGAT‑CTCCTTctggatgg                                                                                                                                                                                                                                         <  2:517905/250‑1 (MQ=255)

ccATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTctggctgg                                                                                                                                                                                                                                         <  1:507518/250‑1 (MQ=255)

     tgtcAGGCACATTATGCAAGCATGTCTGGAACACTTTATTACCCAACCTACCGTGTATTCATGGATGGCGGTGGTGTTGGTGGACTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATGGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGgctgggctgg                                                                                                                                                                                                                                    <  2:266521/247‑1 (MQ=255)

        cAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CGCCTTCTGGCTGGGCTGGCG‑tt                                                                                                                                                                                                                               <  1:279793/251‑1 (MQ=255)

              caTTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTTCCGTGTATTCATTGATGGCGGTGGTGTTTGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTaaaa                                                                                                                                                                                                                         <  1:649164/251‑1 (MQ=255)

                     cAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGtt                                                                                                                                                                                                                  <  1:545384/251‑1 (MQ=255)

                      aaGCATTGCTGGAACACTGTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGGGGTTAAGTTT‑TGGGCCGCCTGGCTGGGAGGGGTTGGCGGGTTT‑CT‑T‑CTGGGGGACTGGAT‑TTCCTTCTGGCTGGGGCGGCGGTTTTAAAAA‑CCGTTg                                                                                                                                                                                                                 >  1:542225/1‑251 (MQ=255)

                           ttgcgtgAACACTTTTTTACCCAAGCTAGCTTTTATTCATTGATGTCTGTGGTTGTGGTGGCCTTTCTGGTGTCGCTGGCGCTGGTCGGTTTTTGTCTTCCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGTCAGCGGCGAG‑TTAAGTTTCTGGCACACCTGGCTGTCAGGGATTG‑TCGGTTG‑TC‑TGCTGGTCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCAt                                                                                                                                                                                                              <  2:787465/243‑1 (MQ=255)

                             gCTGGAACATTTTATTACCCAATCTACCGTGTATGCATTGATGGCGTGGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGTTTTGATTCTACCCGGTGCGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGG‑TG‑GTCTGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCT‑TTTTAAAAAGCCGTTGCATCGCt                                                                                                                                                                                                          <  2:24023/251‑1 (MQ=255)

                                ggataacgttatttgcagatctcccGTGTAGTCATTGGTGGCGGTGGTGTTGGTGGCCTTTCTGGAGGCTCTGGCGCTGGTCGGTTTGATTCTGCCCGGGACGGGGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACTCATGGCTGGCAGGGATTG‑TCGGTTGTCT‑T‑CTGGGCGACTGGAT‑CGCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGt                                                                                                                                                                                                       <  2:422161/228‑1 (MQ=255)

                                   atacttgttaaCCCAATCGTCCGTGTATTCATTGATGGCGGTGGGGTTGGGGGCCTGTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAATTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCTCTGGTCAt                                                                                                                                                                                                    <  2:383256/241‑1 (MQ=255)

                                    cacTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCAtt                                                                                                                                                                                                   <  1:665083/251‑1 (MQ=255)

                                        ttattaCCCAATCTACGGTGTAGTCGTTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCt                                                                                                                                                                                                <  2:361317/250‑1 (MQ=255)

                                          attaCCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTgaa                                                                                                                                                                                             <  1:559130/251‑1 (MQ=255)

                                           ttacccatactaCCGTGTATTCATTGATGTTGGTGGTGTTGGTGGCCTGTCTGGAGTCGCGGGCGCTGGGCGGTTTGATTCTACCCGGTACGGTGCTGAGGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTgaa                                                                                                                                                                                             <  2:914917/239‑1 (MQ=255)

                                                      aCCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGc                                                                                                                                                                                 <  1:390593/251‑1 (MQ=255)

                                                        cGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCat                                                                                                                                                                               <  1:292347/251‑2 (MQ=255)

                                                         gtgtATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTGCGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCatt                                                                                                                                                                              <  1:390956/251‑3 (MQ=255)

                                                          tgtATTCGTTGATGGCGGTGGTGTTGGTGGCCTTTCTGTGGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGAGTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCatt                                                                                                                                                                              <  2:838870/250‑3 (MQ=255)

                                                           gtATTCATTGATGGTGGTGGTGTTGGTGTCCTTTCTGGAGTCGCGGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAG‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCatta                                                                                                                                                                             <  1:978895/250‑4 (MQ=255)

                                                             agtCATTGATGGCTGTGGGGTTGGGGGCCTTGCGTGAGTCGCGGGCGCTGGTCGGTTTGATTCCACCCGGTACGGTTCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGAGGCGAG‑TTAAGTTTCTGGGACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCTTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCattactc                                                                                                                                                                          <  2:312744/249‑2 (MQ=255)

                                                               tCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGGTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCattactcg                                                                                                                                                                         <  1:774660/250‑3 (MQ=255)

                                                                cATTGATGGCGGTGGTGTTGGTGGTCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGAGGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATATCTGAAGAAAAACAAAGCATTACTCGAt                                                                                                                                                                       <  2:693328/251‑1 (MQ=255)

                                                                cATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGAt                                                                                                                                                                       <  1:299936/251‑1 (MQ=255)

                                                                 aTTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATa                                                                                                                                                                      <  1:370362/251‑1 (MQ=255)

                                                                   ggagggggggtgtgtTGGTGGCCTTGCTGGAGTCGCTGGCTCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCTACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATaa                                                                                                                                                                     <  2:935048/239‑1 (MQ=255)

                                                                        gCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCGGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGa                                                                                                                                                               <  1:967714/251‑1 (MQ=255)

                                                                          ggtggtGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTTGGCGACTGGAT‑CTCCTTCTTGCTGGGCTTGCG‑TTTTTAAAAACCGTTGCATCGCTTGTCCTTTTTTAAGAAAAACAAAACATTACTCGATAAAACTgcac                                                                                                                                                             >  1:431060/1‑248 (MQ=255)

                                                                             ggtgTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGGTTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTA‑TTGGCTG‑CT‑TGATGGGCGACTGGAT‑TTCTTTCTGGCTGGGTTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACAt                                                                                                                                                           <  1:141123/250‑1 (MQ=255)

                                                                             ggtgTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATg                                                                                                                                                          <  1:106690/251‑1 (MQ=255)

                                                                                   ggtggCCTTTCTGGGGTCGCGGGCGCTTGTCGGTGTGATTCTACCCGGGACGGGGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑GTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGttt                                                                                                                                                     <  2:410135/250‑2 (MQ=255)

                                                                                       gCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCa                                                                                                                                                <  1:698445/251‑1 (MQ=255)

                                                                                        ccTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGGTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGGG‑TTTTAAAAAGCCGTTTCATCGCTGGTCATTTTTGAAGAAAAAAAAAGCATTACTCGATAAAACTGAACAAGCGTTGCATCa                                                                                                                                                >  1:876822/1‑250 (MQ=255)

                                                                                              gggAGTCGCGGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGc                                                                                                                                         <  1:50472/250‑1 (MQ=255)

                                                                                                      ctggcgctggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGGTTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGGTGGCG‑TTTTTAAAAGCCGTTGCATCGCTGGGCATTTCTGTAGAAAAAAAAAGCATTACTCGATAAAAATGAACATGCGTTTCATCAACAAAGCATGTTC‑Ac                                                                                                                                >  1:203310/1‑251 (MQ=255)

                                                                                                             tggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGGG‑GTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGGT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGACGTTGCATCGCTGGTCATTTCTTAAGAAAAACAAAGCATTACTCGGTAAAACTGAACATGCGTTGCATCCAAACAGCATGTTT‑ACCATTCTg                                                                                                                         >  1:394733/1‑251 (MQ=255)

                                                                                                              ggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCt                                                                                                                          <  1:766355/249‑1 (MQ=255)

                                                                                                               gTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTggt                                                                                                                       <  1:129597/251‑1 (MQ=255)

                                                                                                                 cGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTggtc                                                                                                                      <  1:410903/250‑1 (MQ=255)

                                                                                                                  ggTTTGATTGTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGTCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGATCGTCTT‑CTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTTATCATGCGTTGCATCATCACAGCATTTTC‑ACCATTCTggtcgg                                                                                                                    <  1:778830/251‑1 (MQ=255)

                                                                                                                       gATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGgtcggtcgc                                                                                                                <  1:917478/250‑2 (MQ=255)

                                                                                                                          tCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGcttt                                                                                                             <  1:903995/250‑1 (MQ=255)

                                                                                                                             aCCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTttgtt                                                                                                          <  1:843308/250‑1 (MQ=255)

                                                                                                                                         gCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGt                                                                                              <  1:704680/250‑1 (MQ=255)

                                                                                                                                          gtGATGGCGGGGCTGGGGGCGCTGTGTGGCAGCGGCGAG‑TGAAGTTTCGGGCACTCCTGGCTGGCAGGGATTG‑TCGGTTG‑GC‑TGCTGGGTGACTGGAC‑CTCCGTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACAGGCGTTGCATCAACCCAGCATGTTC‑ACCATTCTGGTCGGGGGCTTTGTTGGCCCGTCGCGTcc                                                                                            <  2:926307/250‑1 (MQ=255)

                                                                                                                                          cgtagggCGGGACTTGGAGCCCTGATTGGCAGCGTCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGGAGGGAGTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTCCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATAACTCTATAAAACTGATCATGCGTTGCATCAACACAGCATGTTC‑CCCATTCTGGTCGGTCCCTTTGTTGGCCCGACGTGTcc                                                                                            <  2:475029/246‑1 (MQ=255)

                                                                                                                                                  ggggCTGGGAGCGCTGATTGGCAGCGGCGCG‑TTAAGTTTCTCGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGc                                                                                    >  1:756836/1‑251 (MQ=255)

                                                                                                                                                  ggggCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGc                                                                                    >  1:882472/1‑251 (MQ=255)

                                                                                                                                                    ggCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGcc                                                                                   >  1:344918/1‑250 (MQ=255)

                                                                                                                                                    ggCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCa                                                                                  <  1:839365/251‑1 (MQ=255)

                                                                                                                                                         ggAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACACCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCCTTTCTGAAGAAAAACCACGCATTAATCGATAAAAATGAACATGCGTTGCTTCAACCCAGCATGTTCCACC‑TTCTGGTCGGTCGCTTTTTTGTACCGACGCGACCCCTGGAGACAAtggt                                                                             >  2:865995/1‑251 (MQ=255)

                                                                                                                                                           aGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAtggtgg                                                                           <  1:358915/251‑1 (MQ=255)

                                                                                                                                                                tGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGGTTG‑TCGGTTGTCT‑T‑CTGGGCGACTGGAT‑ATCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCCGGTCATTTCTGAAGAAAAACAAAGAATTACTAGATAAAACTGAACATGCGTTGCATAAACACACCCTTTTC‑ACCATTCTGGTCGGTCGCTTTTTTGGCCCGCCGCGGCCGCTGGTGCCAATGGTGGCgggg                                                                      >  2:572563/1‑250 (MQ=255)

                                                                                                                                                                 gATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCggg                                                                       <  1:84598/249‑1 (MQ=255)

                                                                                                                                                                   ttggtagtggtgAG‑TTAAGTTTTGGGCACGACTGGCTGGCAGGGAGTG‑TCGGTTT‑TT‑TGCTGGGCGACTGGAT‑CGCCTTCTGGCTGGGCTGGAG‑TTTTAAAAAGCCGTTGCATAGCTGGTCATTTCTGAAGAAAAACAAAGTATTTCTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAAt                                                                    <  2:314580/239‑1 (MQ=255)

                                                                                                                                                                    tGGCAGCGGCGAG‑TTAAGTTTCTTGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACAGGCGTTGCATCAACACAGCATGTTC‑ACCATGCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATg                                                                   <  1:909459/250‑1 (MQ=255)

                                                                                                                                                                     ggCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTGCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATGACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCt                                                                 <  2:935128/251‑1 (MQ=255)

                                                                                                                                                                       gaGCGGCGAT‑TTAAGTTTCTGGCACGCCTGGCTGGCGGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTgg                                                               <  2:671891/250‑1 (MQ=255)

                                                                                                                                                                         gcggcgAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGAt                                                             <  1:154821/251‑1 (MQ=255)

                                                                                                                                                                         gcggcgAG‑TTAAGATTCTGGCACGCCTGGCTGGCAGGGATTG‑TAGGTTG‑TA‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACACGATAAAACTGAACCTGCGATGCCTCAACACAGCCTGTTC‑ACCCTTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGAGGCGGGaagggtggg                                                              >  2:920128/1‑243 (MQ=255)

                                                                                                                                                                          gggtgAG‑TTAAGTTTCTGGCACGCCTGGTGGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATc                                                            <  2:588197/247‑1 (MQ=255)

                                                                                                                                                                            gcgAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTg                                                          <  1:502289/251‑1 (MQ=255)

                                                                                                                                                                            gcgAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTg                                                          <  1:271930/251‑1 (MQ=255)

                                                                                                                                                                              gAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGcc                                                        <  2:225136/251‑1 (MQ=255)

                                                                                                                                                                               aG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAAGGCTGGAtattccg                                                       >  2:553288/1‑245 (MQ=255)

                                                                                                                                                                                         tCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAt                                                                                >  1:55119/1‑217 (MQ=255)

                                                                                                                                                                                         tCTGGCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAt                                                                                <  2:55119/217‑1 (MQ=255)

                                                                                                                                                                                             gCACGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGGTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAAttca                                          <  2:876822/251‑3 (MQ=255)

                                                                                                                                                                                               aCGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGGATGATGGGTTTGCCGGGGGccaacttcatt                                        >  2:266385/1‑241 (MQ=255)

                                                                                                                                                                                               aCGCCTGGCTGGCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATtcatt                                        >  1:799652/1‑251 (MQ=255)

                                                                                                                                                                                                 gCCTGGCTGGCAGGGCTTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATTCTGGATCTGCCGGGGGCAAAATTCATTAc                                      >  1:735934/1‑251 (MQ=255)

                                                                                                                                                                                                   ctggctggCAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGGATGCTGGATCTGCCGGGGGCAAAATTCATTACGc                                    >  2:663762/1‑251 (MQ=255)

                                                                                                                                                                                                     ggctggcAGGGATTG‑TCGGTTG‑TC‑TGCTGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTAGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGACCGACGCGTCCGCTGGTGCCAATGGCGGCGGGAATGCTGGGTCTGCCGGTGGCAAAAttcaatccgccc                                  >  1:919264/1‑241 (MQ=255)

                                                                                                                                                                                                           tggcagggattgtcggttgtctgctGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCACAATTCATTACGCCGAat                               >  1:830481/25‑251 (MQ=255)

                                                                                                                                                                                                            ggcagggattgtcggttgttagctGGGCGACTGGAT‑CTCCTTATGGCTGGGATGGCG‑GTTTAAAAAGCCGTTGCATCGCTGGTCATGTCTGAAGAAAAACAAATCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGGCGGGCGCTTTGTGGGCCCGACGCGTCCGCTGGTGCCAATGGGGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAata                              <  2:216042/228‑1 (MQ=255)

                                                                                                                                                                                                            ggcagggattgtcgggtgtctgctGGGCGACTGGAT‑CTCCTTCGGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAATCATTACGCGATAAAACTGAACAGGCGTTGCATCAACACAGCATGTGC‑GCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAata                              <  2:47003/228‑1 (MQ=255)

                                                                                                                                                                                                              cagggattgtcggttgtctgctGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTTGATCTGCCGGTGGCAAAATTCATTACGCCGAAtatt                            <  1:542979/230‑1 (MQ=255)

                                                                                                                                                                                                                      gtcggttgtctgctGGGCGACTGGAT‑CTCCTTCTGGATGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGct                     <  1:276296/237‑1 (MQ=255)

                                                                                                                                                                                                                        cggttgtctgctggtcGACTGGAT‑CTCCTTCGGGCGTGGCTGGCG‑TTTTAAAA‑GCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGGTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGctgcct                 <  2:479180/236‑1 (MQ=255)

                                                                                                                                                                                                                                 gctGGGCGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCtgt            <  1:898311/246‑1 (MQ=255)

                                                                                                                                                                                                                                       cGACTGGAT‑CTCCTTCTGGCTGGGCTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATCTTATCGGCTGCCTGCTGTGGCCACCGt   >  1:592820/1‑251 (MQ=255)

                                                                                                                                                                                                                                        gattGGAT‑CTCCTTCTGGCTGGGCTGGTT‑GTTTAAAATGCCGTTGCATCGCTGGGCATTTCTTAAGAAAAACAAAGCATTCCCCGATAAAACTGAACATGCTTTGCATCAACAAAGCATGTTC‑ACCATTCTGGGCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAGGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgcaaccgtt  <  2:657920/248‑8 (MQ=255)

CCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAG‑TTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTA‑TTGGCTG‑CT‑TGATGGGCGACTGGAT‑TTCTTTCTGGCTGGGTTGGCG‑TTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTC‑ACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTT  >  NC_000913/71333‑71811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`