Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,175	A→G	R263R (CGA→CGG)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,175	0	A	G	99.0%	334.5 / NA	99	R263R (CGA→CGG)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (38/60);  minor base T (1/0);  total (39/60)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑CATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGT  >  NC_000913/70934‑71412                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                gTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                                                                                                                                                                                                         >  1:816091/1‑250 (MQ=255)   cAGTACATCAACGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCAGGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCTAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa                                                                                                                                                                                                                                      <  1:727419/251‑1 (MQ=255)        cagcAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTGGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCGGGCGCGAGTACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑a                                                                                                                                                                                                                                <  2:750493/248‑1 (MQ=255)          tCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑Acc                                                                                                                                                                                                                              >  1:475029/1‑251 (MQ=255)           cAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑Acc                                                                                                                                                                                                                              <  1:734044/250‑1 (MQ=255)             gCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCggg                                                                                                                                                                                                                           <  1:643800/251‑1 (MQ=255)              cGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTAGCGGGTATTTAAAAAATGC‑ACCgggg                                                                                                                                                                                                                          >  1:28749/1‑251 (MQ=255)                        gaaGACAGCAATTTTGATATCGCCAGCGTCGAACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACCTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGCGGACCACCGTCAC‑AGCCAGGCGAAGCTGCTTTTGAGCCCCCCCCGGATGCCAATCGCCACAGACGGTCGCAATGCTGGTTTTGCCGATCAACTCTATTTATCACGGGTATTTCAAAAAAGC‑ACCGGGGCGAGCCcg                                                                                                                                                                                                                  >  2:978895/3‑249 (MQ=255)                              aGCAATTTTGATATCGCCAGCGTCGCACAGCCTGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAATCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCCATGTTGGGTTTGGCGACCAACTATATTTTTCGCGGGCATTTAAAAA‑TGCCACCGGGGcaaacccgtgcgtgtt                                                                                                                                                                                                          >  2:774660/1‑236 (MQ=255)                                aaaTTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTCTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGTCCAGCCCGAGCGAGttca                                                                                                                                                                                                        <  1:667265/250‑3 (MQ=255)                                      tGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACACGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTATGACGATCAAATCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCAAGACCGAGCGAGtgcagtgccg                                                                                                                                                                                                  >  1:147989/1‑242 (MQ=255)                                       gATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCg                                                                                                                                                                                                  <  1:908107/250‑1 (MQ=255)                                        atatCTCCCGCGTCGCACAGCATGTTTTCTTGTCGCCGTCGCGGTGGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGACAGCCCGAGCGAGTTCCGTGCCGGt                                                                                                                                                                                                <  2:737889/251‑1 (MQ=255)                                          atCGCCAGCGTCGCCCAGCATGTTTGCTTGGCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCATCG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt                                                                                                                                                                                               <  2:151427/250‑1 (MQ=255)                                          atCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGGATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg                                                                                                                                                                                              >  1:849050/1‑251 (MQ=255)                                               cAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTga                                                                                                                                                                                           >  2:758536/1‑249 (MQ=255)                                                 gCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTGCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa                                                                                                                                                                                       <  1:389857/251‑1 (MQ=255)                                                  cGTCGCACAGCATGTTTGCTTGTCTCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa                                                                                                                                                                                      <  2:122879/251‑1 (MQ=255)                                                  cGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa                                                                                                                                                                                       >  1:6988/1‑250 (MQ=255)                                                     cGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCTCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg                                                                                                                                                                                   <  1:885808/251‑1 (MQ=255)                                                     cGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa                                                                                                                                                                                    <  1:50535/250‑1 (MQ=255)                                                     cGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg                                                                                                                                                                                   <  1:950916/251‑1 (MQ=255)                                                        acaGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGCTCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGGGCCGGTTGTGAAGAAAAAGTg                                                                                                                                                                                 >  2:168785/1‑250 (MQ=255)                                                             aTGTTTGCTTGTCGCTGTCTCGGCTGTCACATCTTTTCCGCCAGCATCTAGGGATTAGCTTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCTCCCGGATGCCTACCGCCGCCGTCGGTCGCAATGTTGGTTGTGACGATCAACGCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat                                                                                                                                                                           <  1:534833/251‑1 (MQ=255)                                                              tGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCCGCCCGAGCGCGTTCCGTGCCGGATGTGAAGAAACCGAGAatgatg                                                                                                                                                                          >  2:839365/1‑251 (MQ=255)                                                                      tGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCg                                                                                                                                                                    <  2:518190/249‑1 (MQ=255)                                                                         cGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTCTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg                                                                                                                                                               <  1:772213/251‑1 (MQ=255)                                                                            cGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg                                                                                                                                                            >  1:422161/1‑251 (MQ=255)                                                                               cgcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc                                                                                                                                                          >  1:770741/1‑250 (MQ=255)                                                                                gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCCCCACCCGGATGCCTATCGCCACCGTCGGTCGCACTGTTGGGTTTGCCGATGCACTCTATTTCTCGCGGGTATTTAAATAATGC‑ACCGGGGCCAGCCCGAGCGAGTTACGTGCAGGTTGTGATGAAAAAGGGAATGATGTCGCAGTCAAGtgttcat                                                                                                                                                        >  2:292347/1‑245 (MQ=255)                                                                                gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt                                                                                                                                                        >  1:175291/1‑251 (MQ=255)                                                                                gcgTCTGTCACATCGTTTCCGGCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGATGACCCACG‑TATCAGCCAGGCGAAGCTGCTGTTGAGCGCCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTCTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCTTGCCGGTTGTGAAGAAAAAGTGAATGATGTATCCGTCAAGTGGTCAt                                                                                                                                                        <  2:163488/251‑1 (MQ=255)                                                                                   tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTAGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt                                                                                                                                                     >  2:555233/1‑251 (MQ=255)                                                                                     tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTg                                                                                                                                                   >  1:90757/1‑251 (MQ=255)                                                                                      gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg                                                                                                                                                  <  1:202937/251‑1 (MQ=255)                                                                                      gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTg                                                                                                                                                   <  1:69499/250‑1 (MQ=255)                                                                                         acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGCGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCCAGTTGTAATAATTGG‑Ta                                                                                                                                               >  2:698445/1‑250 (MQ=255)                                                                                             cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAAAGATGTAGCCGTCAAGTTGTCATAATTGG‑GAACGa                                                                                                                                           >  2:649164/1‑250 (MQ=255)                                                                                               tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCTAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGaa                                                                                                                                          <  2:1453/249‑1 (MQ=255)                                                                                               tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACg                                                                                                                                            <  1:310266/247‑1 (MQ=255)                                                                                                     ccAGCAGGTAGGGATTATCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTTCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGGATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACAAATCAGACaa                                                                                                                                  <  2:397285/251‑1 (MQ=255)                                                                                                       agcagTTAGGGCTTAGCATCTTAAGATGGCGTGAGGACCAACG‑TAACAGCCAGGGGAAGCTGATTTTCGGCACCCACCGGATGCCTATCGCCAACGCCGGTCGCAATGTTGGTTTTGACGATAAACTCTATTTCTCGAGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGCGCTAGTTCCGTGCCGGTTGGGACGGACCAGTGAATGATGTAGCCGTCAGGTTGACATAATTGGGTAA‑GAATCAGACaaat                                                                                                                                >  2:398158/1‑249 (MQ=255)                                                                                                       agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAAtt                                                                                                                                <  1:130801/251‑1 (MQ=255)                                                                                                       agcagTTAGGGATTAGCGTCGTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGGCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAAtt                                                                                                                                <  2:207121/251‑1 (MQ=255)                                                                                                           gTTAGGGATTAGCGTCTTAATCTGGCGCGATGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCGCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACg                                                                                                                            <  2:83401/251‑1 (MQ=255)                                                                                                            ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACg                                                                                                                            <  2:792095/250‑1 (MQ=255)                                                                                                                ggATTAGCGTCTTCAGCTGGCGCGATGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGGTCAACTCTATTTCTCGCGTGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCAGTGCCGGTTGTGAAGAAAAAGAGAATGATGTAGCCGTCAGGTTGTCATAATTGG‑CAACGAATCATCCAATTGaagtcttg                                                                                                                       >  2:279793/1‑244 (MQ=255)                                                                                                                ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTg                                                                                                                       <  2:441370/251‑1 (MQ=255)                                                                                                                ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTg                                                                                                                       <  2:175291/251‑1 (MQ=255)                                                                                                                      gCGTCTTATGCTGGCGCGATGCCCAACG‑TATCAGCCGGGCGAAGCTTCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAGCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTTAAGTTTTCATAATGGG‑TAACGAATCAGACTATTGACGTCTTGACGGa                                                                                                                  <  1:850060/250‑1 (MQ=255)                                                                                                                        gTCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTa                                                                                                               <  1:657897/251‑1 (MQ=255)                                                                                                                         tCTTAAGCTGGCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGt                                                                                                                >  1:70224/1‑249 (MQ=255)                                                                                                                                  ggCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGtt                                                                                                     <  1:758536/251‑1 (MQ=255)                                                                                                                                  ggCGCGAGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGt                                                                                                      <  1:61705/250‑1 (MQ=255)                                                                                                                                    cgcgAAGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTg                                                                                                   <  1:612079/251‑1 (MQ=255)                                                                                                                                       gagtgccAACG‑TATCAGCCAGGCGAACCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCTGCGGTCGCAATGTTGGTTGTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑GAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAg                                                                                                <  2:76587/246‑1 (MQ=255)                                                                                                                                        aGGACCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGa                                                                                               <  1:126207/251‑1 (MQ=255)                                                                                                                                          ggactaacgtatcagccAGGCGAATCTGCTTTTGAGCTCCATCCGGATTCCTAGCTCCACCGTCGTTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATTC‑ACCGGTGCCAGACCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGTCAAGTGACGGCTTGTCGGAGTAGCATAGGGTTTGCAGaa                                                                                              <  2:990605/235‑1 (MQ=255)                                                                                                                                           aCCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATc                                                                                            <  1:704632/251‑1 (MQ=255)                                                                                                                                           aCCAACG‑TATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGAATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATc                                                                                            >  1:787465/1‑251 (MQ=255)                                                                                                                                            ccAACG‑GATCAGCCAGGCGAAGCTGCTTTTGAGCTCCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATcc                                                                                           <  2:932244/251‑1 (MQ=255)                                                                                                                                               aacgtatcagccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCAGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGGTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACCGAGGAGAATAGGGTTTGCAGAATCCCt                                                                                         >  2:665083/12‑251 (MQ=255)                                                                                                                                                      cagccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGt                                                                                  >  1:410135/5‑251 (MQ=255)                                                                                                                                                       agccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTc                                                                                 >  1:691659/4‑251 (MQ=255)                                                                                                                                                        gccAGGCGAAGCTGCTTTTGAGCACCACCCCGATGCCCTTCGCCACCGTCGGGCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACTGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTcc                                                                                <  2:840321/249‑1 (MQ=255)                                                                                                                                                            ggCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGGTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCAttt                                                                            <  2:849050/251‑1 (MQ=255)                                                                                                                                                               gAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGAc                                                                         >  2:358915/1‑251 (MQ=255)                                                                                                                                                                 aGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGGAGCAAAAGTGAATGATGTAGCCGTCAAGTTGTCATACTTGG‑TAACGAATCAGACAATTGACGGCTTGCCGGAGTATCATAGGGTTTGCAGAATACCTGCTTCGTCCATTTgcc                                                                         >  2:898311/1‑247 (MQ=255)                                                                                                                                                                  gctgctTTTGCGCCCCCCCCGGATGCCTATCGCCCCCTACGGTAGCAATGTTGGTGTTTACGATCAACGCTATTTCTCGCGGGTATTTAAAAAAAGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCCTCGTCCATTTGACAgg                                                                      <  2:109091/251‑1 (MQ=255)                                                                                                                                                                  gctgctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAgg                                                                      >  2:299936/1‑251 (MQ=255)                                                                                                                                                                        tttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAGCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGcaca                                                                  <  2:494555/249‑1 (MQ=255)                                                                                                                                                                        attGAGCACCCCCCGGATGCCTAGCGCCACCGTCGGTCGCAATGTTGTTTTGGACGATCAACTCTAGTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTTAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCAATTTGACAGGCACAt                                                                 <  2:389362/249‑1 (MQ=255)                                                                                                                                                                         ttGAGCACCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACAtt                                                                <  1:505726/250‑1 (MQ=255)                                                                                                                                                                          tGAGCACCCCCCGTATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTa                                                               <  1:431367/250‑1 (MQ=255)                                                                                                                                                                            aGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGc                                                            <  1:475322/251‑1 (MQ=255)                                                                                                                                                                              caccaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGTTTCGTCCATTTGACAGGCACATTATGCaa                                                          <  1:486295/247‑1 (MQ=255)                                                                                                                                                                                ccaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGc                                                        <  1:168785/249‑1 (MQ=255)                                                                                                                                                                                  ccccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCAt                                                      <  2:475397/250‑1 (MQ=255)                                                                                                                                                                                   cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCATCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCAtt                                                     >  1:383256/1‑251 (MQ=255)                                                                                                                                                                                           ccTATCGCCACAGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGGGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACCGGCACATTATGCAAGCATTACTGGAac                                             >  2:967714/1‑251 (MQ=255)                                                                                                                                                                                             gaTCGCCACCGTCGGTCGCAATGTTGTTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGTCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGGTGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGGCCATTTGACAGGCACATTATGCAAGCATTGCTGGAacac                                           <  2:575514/250‑1 (MQ=255)                                                                                                                                                                                                   cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTAGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTttatt                                     <  1:368124/251‑1 (MQ=255)                                                                                                                                                                                                     ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCAGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTTTGCAAGCATTGCTGGAACACTTTATTAc                                   <  1:162372/251‑1 (MQ=255)                                                                                                                                                                                                          ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAAt                              >  2:129597/1‑251 (MQ=255)                                                                                                                                                                                                           gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGCCGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCCCATTATGCAAGCATTGCTGGaa                                              >  2:690492/1‑234 (MQ=255)                                                                                                                                                                                                           gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGaa                                              <  1:690492/234‑1 (MQ=255)                                                                                                                                                                                                           gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATc                             >  1:809574/1‑251 (MQ=255)                                                                                                                                                                                                               cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTAcc                         >  2:817870/1‑251 (MQ=255)                                                                                                                                                                                                                aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATCATTGG‑AAACGAATCAGACAATTGACGGCTTGACGGAGACGCCTAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACCTTCTGCACGCATTGCTGGAACACTTTATTACACAATCTACCg                        >  2:917478/1‑251 (MQ=255)                                                                                                                                                                                                                aaTGTTGGTTTTGACGATCAACGTTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTTGAACACTTTATTACCCAATCTACCg                        <  2:712257/251‑1 (MQ=255)                                                                                                                                                                                                                 aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGACTGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATACCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTCTTACCCAATCTACCgt                       >  2:84598/1‑251 (MQ=255)                                                                                                                                                                                                                             aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGAt           <  1:618891/251‑1 (MQ=255)                                                                                                                                                                                                                             aCGATCAACTCTAGTTCGCGCTGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGATTTCCGTGCCGGTTGTGAATAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTTACGTAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCAtttat           <  1:618886/251‑4 (MQ=255)                                                                                                                                                                                                                              cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCACTCTACCGTGTATTCATTGATg          >  2:507518/1‑251 (MQ=255)                                                                                                                                                                                                                                  cAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCgg      <  1:814199/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaCTCTATTTCTCGCGGGTATTTAAAACATGC‑ACCGGTGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACTAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCggt     <  2:663476/251‑1 (MQ=255)                                                                                                                                                                                                                                     ctctATTTCTCGCGGGTATTTAAAACATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCggtgg   >  1:693328/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCggtggt  <  1:491342/248‑1 (MQ=255)                                                                                                                                                                                                                                         aTTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAAGCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCggtggt  <  2:289789/248‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                GTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACG‑CATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGT  >  NC_000913/70934‑71412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑