BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,082	T→C	I232I (ATT→ATC)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,082	0	T	C	99.0%	325.5 / ‑4.3	105	I232I (ATT→ATC)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (46/57); total (47/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 105 total aligned reads displayed.

AAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGC‑ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCC  >  NC_000913/70845‑71323

  aTCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCACCGTATCAGCCAGGCGAAgctg                                                                                                                                                                                                                                       >  2:910336/1‑251 (MQ=255)

    cTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGAATCAGCCAGGCGAAgctgat                                                                                                                                                                                                                                     >  2:545384/1‑249 (MQ=255)

        ttGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTg                                                                                                                                                                                                                                 >  1:663476/1‑251 (MQ=255)

           ggCAAGTGTTACTGCGGCGCATGGAAGGGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGGCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGc                                                                                                                                                                                                                              <  2:458258/250‑1 (MQ=255)

             cAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGGCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑Ac                                                                                                                                                                                                                           >  1:1453/1‑251 (MQ=255)

               aTTGTTACTGCGGCGCATGGAAGAGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCCTGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAATTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTgggctccc                                                                                                                                                                                                                          >  2:618891/1‑244 (MQ=255)

               aTTGTTACTGAGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATAAGAGATCACATGGCAGACAGCAATTTTGATATCGACAGCGTCGCACAGACTGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGTCCAACGAATCAGCCAGGAGAAGCTGCGTTTGAgtaccc                                                                                                                                                                                                                          >  2:618886/1‑246 (MQ=255)

                ttGTTACTGCGGCGCATGGAAGCGATTAACGCGTCGCTCCATACACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTTGCCGTCGCGTCTACCACATCTTTTTCGCCAGCTGTTAGGGATTAGCGTCTTAATATGGCGCGAGGCCCAACGTATCAACCACGCAAAACTGCTTTTGAGC‑Accac                                                                                                                                                                                                                        >  2:276296/1‑248 (MQ=255)

                 tGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑accacc                                                                                                                                                                                                                       >  1:636770/1‑251 (MQ=255)

                 tGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑accacc                                                                                                                                                                                                                       >  1:475397/1‑251 (MQ=255)

                   ttACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCg                                                                                                                                                                                                                     >  1:289789/1‑251 (MQ=255)

                      cTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGAGAGGCCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCggct                                                                                                                                                                                                                  >  2:475322/1‑249 (MQ=255)

                       tGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCCAGGCTTGTCCGCACATCAGCGATCACCTGGCCGACAGCACTTTTGATATCGCCAGCGTCGCAAAGCATGTTTGCTTGTAGCCGTCGCGTCTGTCCCATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGACGCTGCTTTTGAGC‑ACCACCCGGATg                                                                                                                                                                                                                 >  1:914652/1‑251 (MQ=255)

                          ggcgCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCCGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACCTCTTTTCCGCCCGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCt                                                                                                                                                                                                              >  2:950916/1‑251 (MQ=255)

                          ggcgCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCt                                                                                                                                                                                                              <  1:447625/251‑1 (MQ=255)

                               aTGGAAGCGGTTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATAACCTGGAAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCAGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGGATCAGCCAGGCGAAGCTGCTTTTGAGA‑ACCAACCGGATGCCTATCGc                                                                                                                                                                                                         >  2:505726/1‑251 (MQ=255)

                                      ggATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCGTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTAGCACAGCATGTTTGCTTGTCGCCGTCGCGTCGGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGTCTATCGCCACCgtc                                                                                                                                                                                                  <  2:375459/250‑1 (MQ=255)

                                       gATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGTATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCgtcg                                                                                                                                                                                                 >  1:609489/1‑251 (MQ=255)

                                           aacgagtcgatccactaacggATGGATAATCGGGTACGCGATGCTTGTCAGTACATCAGGGATCACCTGGCGGACATCAATTTTGATATCGCCAGCGTCGCATAGCATGTTTGCTTGTCGCCGTCTAGTCTGTCGCATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCGTGTGAGC‑ACCACCCGGATGCCTATCGCCACCgtcggtc                                                                                                                                                                                              <  2:100365/230‑1 (MQ=255)

                                           aaCGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCTTGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGC‑ACTACCCGGATGCCTATCGCCACCgtcggtc                                                                                                                                                                                              <  1:417148/250‑1 (MQ=255)

                                           aaCGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCgtcggtc                                                                                                                                                                                              <  1:730199/250‑1 (MQ=255)

                                           aaCGAGTCGCTCCAGCCACCGATGGATAATCGGGTACTCGAGGCTTGTCAGTACATCAGCGATCACCGTGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACAGCTTGTCCGCCAGCATTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGTTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCgtcggtc                                                                                                                                                                                              <  2:600932/250‑1 (MQ=255)

                                             cGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCa                                                                                                                                                                                           <  1:110829/251‑1 (MQ=255)

                                              gagccgcactatccaCCGAGGGATGTTAGGGTACGCGAGGCTTGTCAGTACACCAGCGATCGTCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTTTCGCCGTCGCTTCTGCCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGTCCAACGTACCAGCCAGGCGAAGCTGCTTTTGATC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCaa                                                                                                                                                                                          <  2:665596/241‑1 (MQ=255)

                                                gTCGCTCTATACACCGATGGATAATCGGGTACGCGAGGCTTGACTGTGCATCAGCGATCACCTGTCAGCCAGCAATTTTGATATTGCCCGCGTCGCACTCCATGCTTGCTTGTCGCCGTCCCGTCTGATACCTCTTTACCGCCAGCCGTCAGCGATGAACGTCTTAATCTGGCGCGTGGACCAACGGATCAGCAAGGCGAAGCTGCTTTTGAGC‑ACCACCCTGAGGCCAATCGCCACCGTCGGGCGCAATg                                                                                                                                                                                        >  2:390956/1‑251 (MQ=255)

                                                gTCGCTCCATCAACCGATGGATAATCGTGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGAAAGCAATTTTGATCTCGCCAGCGTCGCACATCATGTTTGCTTGTCGCCATCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGCTTAGCGTCTTAAGCTGGCGCGAGGCCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCCAC‑ACCCGGATGCCTATCGCCCCCGTCGGgcgccatt                                                                                                                                                                                        >  2:618818/1‑247 (MQ=255)

                                                   gCTCCATCTACCTAAGGATAAGCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGTCAGCAATTTTGATAGCGACAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg                                                                                                                                                                                     <  2:208750/251‑1 (MQ=255)

                                                     tccatccaCCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGCCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGt                                                                                                                                                                                   >  1:46354/1‑251 (MQ=255)

                                                     cccatccaCCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCGGGCAGACAGCAATTTTGATATCGCCATCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGt                                                                                                                                                                                   <  2:286362/250‑1 (MQ=255)

                                                      ccatccaCCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt                                                                                                                                                                                  >  1:575514/1‑251 (MQ=255)

                                                         cccaCAGATGGTTAATCGGGTACGCTAGGCTTGTCAGTACATCAGCGATCCCCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGGCGCCGTCGCGTCTGTCACATCTTGGCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCTGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGAGGCCGATCGCCCCCGTCGGTCGCAATGTTGGTTTTg                                                                                                                                                                               <  2:125380/250‑1 (MQ=255)

                                                          ccaCCGATGGATAATCGGGTACGCGAGGCTTGCCAGAAAAGCAGCGATCTCCTGGCAGACATCAATTATGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTACCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTg                                                                                                                                                                               <  2:723482/250‑1 (MQ=255)

                                                            aCCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGCTGCCTAGCGCCACCGTCGGCCGCAATGTTGGTTTTGACg                                                                                                                                                                            >  2:314521/1‑251 (MQ=255)

                                                              cGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAt                                                                                                                                                                          <  1:742869/251‑1 (MQ=255)

                                                                    aTAAGCGGGTACGCGAGGCTTGTCAGTTCATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCCCAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGGAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActc                                                                                                                                                                    <  2:738974/251‑1 (MQ=255)

                                                                      aaTCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGGCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGTATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct                                                                                                                                                                   <  2:401758/250‑1 (MQ=255)

                                                                            ggaCGCGAGGCTTGTCAGTACATCAGCGAGCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTGGTCGCCGGCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGAGTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtctc                                                                                                                                                            <  2:364209/249‑1 (MQ=255)

                                                                             tgcgctaGGCTTGTCAGTACCGCAGCGTTCGCCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCGCAGCATGTTTGCTTGTGGCCTTCGCGTCTGTCACAGCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTTGCGCGAGGACCAGCGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCGCCCGGATGCCTAGCGCCGCCGTCGGTCGCAATGTTGGTTTTGACTATCAACTCTATTtctc                                                                                                                                                            <  2:435190/244‑1 (MQ=255)

                                                                             tACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcg                                                                                                                                                           >  1:228556/1‑251 (MQ=255)

                                                                              aCGCGGGGCTTGTCAGTACATACGCGAGCACCTGGCAGGCAGCAATTTTGGTATCGCCAGCGGCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCGGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcg                                                                                                                                                           <  2:164260/250‑1 (MQ=255)

                                                                               cgcgAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgcg                                                                                                                                                         >  1:517905/1‑251 (MQ=255)

                                                                                  gAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgcgggt                                                                                                                                                      <  1:720624/251‑4 (MQ=255)

                                                                                  gAGGCTTGTCAGTACATCAGCGATCACCGGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTcgcgcgggt                                                                                                                                                      <  1:620090/251‑4 (MQ=255)

                                                                                         gTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                                                                                                                >  1:816091/1‑250 (MQ=255)

                                                                                           cAGTACATCAACGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCAGGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCTAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa                                                                                                                                             <  1:727419/251‑1 (MQ=255)

                                                                                                cagcAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTGGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCGGGCGCGAGTACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑a                                                                                                                                       <  2:750493/248‑1 (MQ=255)

                                                                                                  tCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑Acc                                                                                                                                     >  1:475029/1‑251 (MQ=255)

                                                                                                   cAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑Acc                                                                                                                                     <  1:734044/250‑1 (MQ=255)

                                                                                                     gCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCggg                                                                                                                                  <  1:643800/251‑1 (MQ=255)

                                                                                                      cGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTAGCGGGTATTTAAAAAATGC‑ACCgggg                                                                                                                                 >  1:28749/1‑251 (MQ=255)

                                                                                                                      aGCAATTTTGATATCGCCAGCGTCGCACAGCCTGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAATCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCCATGTTGGGTTTGGCGACCAACTATATTTTTCGCGGGCATTTAAAAA‑TGCCACCGGGGcaaacccgtgcgtgtt                                                                                                                 >  2:774660/1‑236 (MQ=255)

                                                                                                                        aaaTTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTCTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGTCCAGCCCGAGCGAGttca                                                                                                               <  1:667265/250‑3 (MQ=255)

                                                                                                                              tGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACACGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTATGACGATCAAATCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCAAGACCGAGCGAGtgcagtgccg                                                                                                         >  1:147989/1‑242 (MQ=255)

                                                                                                                               gATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCg                                                                                                         <  1:908107/250‑1 (MQ=255)

                                                                                                                                atatCTCCCGCGTCGCACAGCATGTTTTCTTGTCGCCGTCGCGGTGGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGACAGCCCGAGCGAGTTCCGTGCCGGt                                                                                                       <  2:737889/251‑1 (MQ=255)

                                                                                                                                  atCGCCAGCGTCGCCCAGCATGTTTGCTTGGCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCATCGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt                                                                                                      <  2:151427/250‑1 (MQ=255)

                                                                                                                                  atCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGGATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg                                                                                                     >  1:849050/1‑251 (MQ=255)

                                                                                                                                       cAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTga                                                                                                  >  2:758536/1‑249 (MQ=255)

                                                                                                                                         gCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTGCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa                                                                                              <  1:389857/251‑1 (MQ=255)

                                                                                                                                          cGTCGCACAGCATGTTTGCTTGTCTCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa                                                                                             <  2:122879/251‑1 (MQ=255)

                                                                                                                                          cGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa                                                                                              >  1:6988/1‑250 (MQ=255)

                                                                                                                                             cGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCTCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg                                                                                          <  1:885808/251‑1 (MQ=255)

                                                                                                                                             cGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa                                                                                           <  1:50535/250‑1 (MQ=255)

                                                                                                                                             cGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg                                                                                          <  1:950916/251‑1 (MQ=255)

                                                                                                                                                acaGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGCTCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGGGCCGGTTGTGAAGAAAAAGTg                                                                                        >  2:168785/1‑250 (MQ=255)

                                                                                                                                                      tGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCCGCCCGAGCGCGTTCCGTGCCGGATGTGAAGAAACCGAGAatgatg                                                                                 >  2:839365/1‑251 (MQ=255)

                                                                                                                                                              tGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCg                                                                           <  2:518190/249‑1 (MQ=255)

                                                                                                                                                                 cGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTCTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAg                                                                      <  1:772213/251‑1 (MQ=255)

                                                                                                                                                                    cGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg                                                                   >  1:422161/1‑251 (MQ=255)

                                                                                                                                                                       cgcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc                                                                 >  1:770741/1‑250 (MQ=255)

                                                                                                                                                                        gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑CCCACCCGGATGCCTATCGCCACCGTCGGTCGCACTGTTGGGTTTGCCGATGCACTCTATTTCTCGCGGGTATTTAAATAATGC‑ACCGGGGCCAGCCCGAGCGAGTTACGTGCAGGTTGTGATGAAAAAGGGAATGATGTCGCAGTCAAGtgttcat                                                               >  2:292347/1‑245 (MQ=255)

                                                                                                                                                                        gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt                                                               >  1:175291/1‑251 (MQ=255)

                                                                                                                                                                        gcgTCTGTCACATCGTTTCCGGCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGATGACCCACGTATCAGCCAGGCGAAGCTGCTGTTGAGC‑GCCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTCTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCTTGCCGGTTGTGAAGAAAAAGTGAATGATGTATCCGTCAAGTGGTCAt                                                               <  2:163488/251‑1 (MQ=255)

                                                                                                                                                                           tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTAGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAt                                                            >  2:555233/1‑251 (MQ=255)

                                                                                                                                                                             tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTg                                                          >  1:90757/1‑251 (MQ=255)

                                                                                                                                                                              gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTgg                                                         <  1:202937/251‑1 (MQ=255)

                                                                                                                                                                              gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTg                                                          <  1:69499/250‑1 (MQ=255)

                                                                                                                                                                                 acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGCGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCCAGTTGTAATAATTGG‑Ta                                                      >  2:698445/1‑250 (MQ=255)

                                                                                                                                                                                     cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAAAGATGTAGCCGTCAAGTTGTCATAATTGG‑GAACGa                                                  >  2:649164/1‑250 (MQ=255)

                                                                                                                                                                                       tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCTAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGaa                                                 <  2:1453/249‑1 (MQ=255)

                                                                                                                                                                                       tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACg                                                   <  1:310266/247‑1 (MQ=255)

                                                                                                                                                                                             ccAGCAGGTAGGGATTATCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTTCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGGATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACAAATCAGACaa                                         <  2:397285/251‑1 (MQ=255)

                                                                                                                                                                                               agcagTTAGGGCTTAGCATCTTAAGATGGCGTGAGGACCAACGTAACAGCCAGGGGAAGCTGATTTTCGGC‑ACCCACCGGATGCCTATCGCCAACGCCGGTCGCAATGTTGGTTTTGACGATAAACTCTATTTCTCGAGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGCGCTAGTTCCGTGCCGGTTGGGACGGACCAGTGAATGATGTAGCCGTCAGGTTGACATAATTGGGTAA‑GAATCAGACaaat                                       >  2:398158/1‑249 (MQ=255)

                                                                                                                                                                                               agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAAtt                                       <  1:130801/251‑1 (MQ=255)

                                                                                                                                                                                                   gTTAGGGATTAGCGTCTTAATCTGGCGCGATGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCGCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACg                                   <  2:83401/251‑1 (MQ=255)

                                                                                                                                                                                                    ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACg                                   <  2:792095/250‑1 (MQ=255)

                                                                                                                                                                                                        ggATTAGCGTCTTCAGCTGGCGCGATGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGGTCAACTCTATTTCTCGCGTGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCAGTGCCGGTTGTGAAGAAAAAGAGAATGATGTAGCCGTCAGGTTGTCATAATTGG‑CAACGAATCATCCAATTGaagtcttg                              >  2:279793/1‑244 (MQ=255)

                                                                                                                                                                                                        ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTg                              <  2:441370/251‑1 (MQ=255)

                                                                                                                                                                                                        ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTg                              <  2:175291/251‑1 (MQ=255)

                                                                                                                                                                                                              gCGTCTTATGCTGGCGCGATGCCCAACGTATCAGCCGGGCGAAGCTTCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAGCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTTAAGTTTTCATAATGGG‑TAACGAATCAGACTATTGACGTCTTGACGGa                         <  1:850060/250‑1 (MQ=255)

                                                                                                                                                                                                                gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTa                      <  1:657897/251‑1 (MQ=255)

                                                                                                                                                                                                                 tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGt                       >  1:70224/1‑249 (MQ=255)

                                                                                                                                                                                                                          ggCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGtt            <  1:758536/251‑1 (MQ=255)

                                                                                                                                                                                                                          ggCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGt             <  1:61705/250‑1 (MQ=255)

                                                                                                                                                                                                                            cgcgAAGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTg          <  1:612079/251‑1 (MQ=255)

                                                                                                                                                                                                                               gagtgccAACGTATCAGCCAGGCGAACCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCTGCGGTCGCAATGTTGGTTGTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑GAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAg       <  2:76587/246‑1 (MQ=255)

                                                                                                                                                                                                                                aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGa      <  1:126207/251‑1 (MQ=255)

                                                                                                                                                                                                                                   aCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATc   <  1:704632/251‑1 (MQ=255)

                                                                                                                                                                                                                                   aCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGC‑ACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGAATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATc   >  1:787465/1‑251 (MQ=255)

                                                                                                                                                                                                                                    ccAACGGATCAGCCAGGCGAAGCTGCTTTTGAGC‑TCCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATcc  <  2:932244/251‑1 (MQ=255)

AAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGC‑ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGC‑ACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGG‑TAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCC  >  NC_000913/70845‑71323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`