Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,581	C→T	V65V (GTC→GTT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,581	0	C	T	96.6%	261.7 / ‑3.5	90	V65V (GTC→GTT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (52/34);  total (54/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGC  >  NC_000913/70335‑70822                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       gATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGTTTGAGGCCAACGGTTATTTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCTGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGcc                                                                                                                                                                                                                                                    >  1:224758/1‑251 (MQ=255)          tGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGGAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGGGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCggggg                                                                                                                                                                                                                                           >  1:241931/1‑249 (MQ=255)           agACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAAAGCCGATTTAGGCCCACGGGTTTTTCGGTTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTTTCAATCTCA‑CCATTCGCGGGCAGGGGGTGG‑GGAAAAATCAGGGACGAGAATTTTTTTGCCGACCgggggg                                                                                                                                                                                                                                          >  1:45825/2‑248 (MQ=255)             aCAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGata                                                                                                                                                                                                                                       <  2:945614/251‑1 (MQ=255)                    gTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGCAAGGGTATATTCTCAATCTCC‑CCATTCGCGGGCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGAAATTTT‑GCt                                                                                                                                                                                                                               >  1:976612/1‑251 (MQ=255)                       tcttctCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGTGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGt                                                                                                                                                                                                                             <  1:749800/250‑1 (MQ=255)                                   tgcgggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGCTTACCGCCGATTGAGGCCAACGGTTATCTCGCTTTTTTTATCGACCGACCGCTGGGAATGCAAGGTTTTATTCTAGAACTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCCGGGACAGGAATTTTTTTGCCGACCGGG‑TGCTATTTT‑GCTGTTTCAGC‑CAgg                                                                                                                                                                                                                  >  1:446667/4‑248 (MQ=255)                                   ggcgggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGCCCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCC‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAgg                                                                                                                                                                                                                  >  1:271110/4‑248 (MQ=255)                                           tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATtcatc                                                                                                                                                                                                       >  1:757277/1‑251 (MQ=255)                                                       ggCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCCACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGATATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGgtcgtc                                                                                                                                                                                           >  2:401930/1‑251 (MQ=255)                                                         atGAAGCGCAAAATGATCCCATGCTGCCGGGATACTCGATTAATGCCAATCTGGTGGCGGGTTTAGCGCCGAATGAAGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGGATGACAGGTTATATTCTCAATATCA‑CCATTCGCGGGCAGGGGGGGG‑TGAAAAATCAGGGACGCGAATTTGTTTGCCGCCCGGG‑TGGTATTTT‑GCTGTTCCCGC‑CAGGCGAGATTCATCACTCCGGTCGTCAt                                                                                                                                                                                         >  2:594239/2‑251 (MQ=255)                                                           gAAGCGCAAAATTACCCCCTGCTGCCGGGATACTCGTTTAATTCCCATCTGGTGGCGGGTTGAACGCCGATTGAGGCAATCGTTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTGGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCAt                                                                                                                                                                                         <  2:45825/249‑1 (MQ=255)                                                           gAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGTGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATc                                                                                                                                                                                        <  1:698269/250‑1 (MQ=255)                                                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGGGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTcg                                                                                                                                                                              >  1:163488/1‑251 (MQ=255)                                                                       gATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCTCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑GGATATTTT‑GCTGTTCCCGC‑CAGGGGAGATTCATCACTACGGTCGTCATCCGGAGACTcgcg                                                                                                                                                                            >  2:72119/1‑250 (MQ=255)                                                                          ccccTGCTGCCGGGATACTCGTTTATTGCCCATCTGGGGGCGTGTTTAACGCCGATTGAGGCCAACGTTTATCTCGATTTTTTTTTCGACCGACCGCGGGGAATGAAAGTTTATATTCCCAATCGCC‑CCTTTCGCGTTCATGGGTTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGCCCGGG‑TGTTATTTG‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGC‑ATg                                                                                                                                                                       <  2:924787/251‑1 (MQ=255)                                                                          ccccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTTCCTCGATTTTGTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCACA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATg                                                                                                                                                                       <  2:241931/251‑1 (MQ=255)                                                                          ccccGGCTGTCGGGATACTCTTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACGGACCGCTGGGATTGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAAGCGGGGACGAGAATTTGTTTGCCGACCGGT‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATg                                                                                                                                                                       <  1:518670/251‑1 (MQ=255)                                                                              tgctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATggcat                                                                                                                                                                   >  1:723482/1‑248 (MQ=255)                                                                                     gggATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATggaatacccagg                                                                                                                                                            >  2:99942/1‑241 (MQ=255)                                                                                      ggATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGCCCGCTGGGGATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGTAAAATAAGGGACGAGTATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGCTTCATCACTACGGTCGTCTTCCGGAGGCTCG‑GAGATGGTATCACC‑AGTg                                                                                                                                                          >  2:370804/1‑251 (MQ=255)                                                                                        aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGGCCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGGGAGATTCATCACTTCGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCAAC‑AGTggg                                                                                                                                                        >  2:841665/1‑251 (MQ=255)                                                                                          aCTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTTAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGCTTCATCACTACGGTCGTCATCCGTGGGATCGCGA‑ATgggatacacagggggtt                                                                                                                                                       >  2:197332/1‑236 (MQ=255)                                                                                          aCTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGGTATTTT‑GCTGTTCCCGC‑CAGGCGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCCCC‑AGTggggt                                                                                                                                                      >  2:366821/1‑249 (MQ=255)                                                                                          aCTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGtt                                                                                                                                                      >  1:503590/1‑251 (MQ=255)                                                                                                  aatgCCCATCTGGTGGCGGGTTTAACGCCGCTTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCAATACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGGGGGCTTACTTTCg                                                                                                                                              >  1:665596/4‑251 (MQ=255)                                                                                                  aatgCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAGGAAAGGTTATATTCTCAATCTCA‑CCATCCGCGGTCAGGGGGGGG‑TGAAAAATCAGGGACGAGAATTTTTTTGCCGGCCGTG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTGGGGA‑ATGGTATCACC‑AGTGGGTTTACTTTc                                                                                                                                               <  1:383798/247‑1 (MQ=255)                                                                                                   atgCCCATCTGGAGGCGGGTTTAACGCCGATTGAGGGCAACGGTTATCTCGGTTTTTTTATCGACCGAACGATGGGAATGAAAGGTTATATTCTCAATCTCA‑TCATTCGCGGTCAGGGGGTGG‑GGAAAAAACAGGAGCGTGAATTAGTTTGCCGACCGGG‑GGAGATTTT‑GCTTTTCCCGCACAG‑AGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGAGT‑ATGGTATCACCCAG‑GGGTTTACTTTcat                                                                                                                                             >  2:861334/3‑249 (MQ=255)                                                                                                     gccagtctggggtggggTTTACCGCCTATGGAGGCCAACGGTTATCACGTTTTTTTTTCCGCCCGCCGGGGGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATGCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGATTTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTcc                                                                                                                                           <  2:600877/237‑1 (MQ=255)                                                                                                     gCCCATCGGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTc                                                                                                                                            <  1:537717/250‑1 (MQ=255)                                                                                                             ggtggCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCcgcgcg                                                                                                                                      <  1:161512/248‑1 (MQ=255)                                                                                                                 gggggTTTAACGCCGTGGGGGGCCAACGGTTAACTCGTTTTTTGTAACGCCCGCCCGCTGGGAATGAAAGTTTATATTCTCAACCTCA‑CCATTCGCGGGCAGGGGGGGG‑TGAAAAATCAGGGACGAGAATTTGTTGGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTg                                                                                                                               <  2:124726/249‑1 (MQ=255)                                                                                                                   gggTTTAACGCCGATTGCGGCCAACGGTTATCTCGATTTTTTTATCGACAGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGGTTACTTTCGTCCGCGCGCCTACTGGc                                                                                                                             >  2:734044/1‑251 (MQ=255)                                                                                                                          aCGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCC‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGAACGAGAATTTGTTTTCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCGTAGGGAGGCTCGCGT‑AAGGTATAACC‑AGTGGGTTTACTTTCGTCCGGGCGCCACCTGGCATGaagg                                                                                                                      >  2:76337/1‑249 (MQ=255)                                                                                                                           cGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATgg                                                                                                                     <  1:841665/251‑1 (MQ=255)                                                                                                                              cGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGGGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCtt                                                                                                                  >  2:281242/1‑251 (MQ=255)                                                                                                                                    agtcaaaCGTTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCACA‑CCATTCGCGGTCAGGGGGTGT‑TGAAAAATCAGGGACGAGAATTGGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGAGTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTgg                                                                                                            <  2:241085/246‑1 (MQ=255)                                                                                                                                       ccAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGAGG‑TGAAAAATAAGGGACGAGGGTTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCTGAGGCTCGCGA‑ATAGTATCACC‑AGTGGGTTTACTTTCGTACGCGCGCCTACTGGCATGACTGGCTTAGCTGGc                                                                                                           >  1:958637/1‑249 (MQ=255)                                                                                                                                         aaCGGTTATCTCGATTTTTTTCTCGACTGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CAATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTACCTGGCCGt                                                                                                        >  2:417148/1‑250 (MQ=255)                                                                                                                                         aaCGGGTATTTCGATTTTTTTATCGACCGCCTCCTGGGAAGTAAAGGTTGAATTCTCAAACTCT‑CCTTTCGGGGTCGTGGGTTGG‑TGAAAAATCAGGGACGTGATTTTGTTGGCCGCCAGGG‑TGA‑ATTTTAGCTGTCCCCGC‑CAGGAGAGATTCATCACTACGGTCGCCATCCGGAGGCTCGCGT‑ATGGTATCACC‑ATTGGGTTTTCGTTCGCCCGCGCGCCTACTGGCATGAATTGCTTAACTGGCCgcc                                                                                                       <  2:579184/251‑3 (MQ=255)                                                                                                                                           aggTTAACTAGATTTTTTAATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑GGAAAAATAAGGGAAGAGAATTTATTTGACGACCGGG‑TGATATTTT‑GCTGTTACCGC‑CAGGAGAGATTAATCACTACGGTCGACATCCGGAGGCGCGCGA‑ATGGTATCACC‑AGAGGGTTTACTTTCGTACGCGGGCATACTGTCTAGCATGGCTTAACTGGCAGTCa                                                                                                      >  2:162372/2‑250 (MQ=255)                                                                                                                                            ggTTATCTCGATTTTTTAATCAACCGACCGCTGGGAATGAAGGGTTATATTCTCAATCTAA‑CCATTCGCGGTCAGGGGGTGG‑TGAAACATCCGGGACGAGCATTTGTTTGCCGACCGGG‑TGATATTAT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCCAGCGC‑ATGGTATCAAC‑AGTGGGTTTACTTTCGTCCGCGCGCCGACTGGCATGAATGGCTTCTCTGGCCGTCaa                                                                                                     >  2:620090/1‑250 (MQ=255)                                                                                                                                               tATCTCGATTTTTTTATCGAACGACCGCTGGGAATGAAAAGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGCGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGACTACTGGCATGAATGAGTTAACAGGCCGTCAATAtt                                                                                                 >  2:885808/1‑251 (MQ=255)                                                                                                                                                    cGATTTTTCAATCGACCGACCGCTTGGAATGAACGGTTATATTCGCAAT‑TCATACATTCGCGGTTAGGTGGTGG‑TGAAAAATAAGGGACGAGAATTTGTCTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTg                                                                                               <  1:284173/248‑1 (MQ=255)                                                                                                                                                     ggtttttttATCGACCGACCGCTGGGTATGAAAGGTTATATTCTCAATCTCA‑TCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGCGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCAGC‑CAGGAGAGATTCCTCACTACGGTCGTCATCCGGAGGCTAGCGA‑ATGGTATCACC‑AGATGGTTTACGTTCGTCCGAGCGGCTACTGGCATGAAACGCTTAACTGACCGTCACTATTTgaca                                                                                            >  2:727419/3‑247 (MQ=255)                                                                                                                                                         tttttATGGCCCGACCGTGGGGAAGGAAAGGTTATATTCTCAATCTTC‑CCATCGGCGGT‑GGGGGGTGGGTGAAAAACAAGGTAGGAGAATTTGTTTGCCGCCGGGG‑TGATATTTT‑TCTTGTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAc                                                                                        <  2:235800/250‑1 (MQ=255)                                                                                                                                                         tttttATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGCCCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAc                                                                                        <  1:221433/250‑1 (MQ=255)                                                                                                                                                             tagcggccgaccgCTGGGATTGAAAGGTTATATTCTCAATCTCC‑CCATTCTCGGTCGGGGGGTGG‑TGAAAAAGCAGGGACGGGAATTTGTGTGCCAACCGGT‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTAATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑ATTGGGTTTACTTTCGTCCGCGCGCCTACTTGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACgggg                                                                                    <  1:767088/244‑2 (MQ=255)                                                                                                                                                              aTCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACggggtt                                                                                  >  1:734419/1‑248 (MQ=255)                                                                                                                                                                  accgaccgCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAAGGGgttcttt                                                                              >  1:435245/1‑251 (MQ=255)                                                                                                                                                                   ccgaccgCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTG‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttc                                                                             <  1:99942/251‑1 (MQ=255)                                                                                                                                                                    cgaccgCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                                            <  1:823378/251‑1 (MQ=255)                                                                                                                                                                     gaccgCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTTTCTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGc                                                                           >  1:220021/1‑251 (MQ=255)                                                                                                                                                                            gggaagaaaaGGTTATATACTCAATCTCC‑CCGTTCGCGGTCAGGGGGGGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg                                                                    <  1:370804/244‑1 (MQ=255)                                                                                                                                                                                 tGAAAGGGTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGAAGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AATGGGTTTACTTTCGTCCGCGCGCATCCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGAGTTCTTTCGCCCGGACGAAgcg                                                               >  2:771795/1‑251 (MQ=255)                                                                                                                                                                                        ttATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTAATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGCATGGCTTACCTGGCCGTCAATATTTGCCAATCCGGGGTTCTTTCGCCCGGATGAAGCGGACCAGc                                                        >  2:593926/1‑251 (MQ=255)                                                                                                                                                                                         tataTTCTCAATCTCA‑CAATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTAATCAATACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGGGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGATTGGCTTAAATGGCCGTCAAAATTTGCCACTACGGGATTCTTTAGCCCGGGTGAAgtcaccaagcc                                                       >  2:643800/1‑241 (MQ=255)                                                                                                                                                                                                 cAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc                                                >  1:230456/1‑250 (MQ=255)                                                                                                                                                                                                  cattttccccATCCGCGGTCAGGGGGGGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGTG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                                 <  1:748518/241‑1 (MQ=255)                                                                                                                                                                                                  aaTCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGCCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc                                                >  1:401758/1‑249 (MQ=255)                                                                                                                                                                                                      tcA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc                                          >  1:458258/1‑251 (MQ=255)                                                                                                                                                                                                       cA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGCCCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGTGGCTCGCGA‑CTGGTATCACC‑AGAGGGTTTACTTTCGCCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTTCCAATACAGGGTTCTTTCGCCCGGATGAAGCGAACAAGCCGCGTTTccactaac                                         >  2:130801/1‑244 (MQ=255)                                                                                                                                                                                                       cA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCCCC‑AGTGGGTTTCCTTTCGTCCGCGCGCCTACTGGCATGACTGGCTTAACTGGCCGTCCATATTTGCCAATACGGGGGTTTTTCGCCCCGATGATGCGCACCAGCCGCCTTTCAGCGAcc                                         >  2:580851/1‑251 (MQ=255)                                                                                                                                                                                                        a‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGTGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGACATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCt                                        >  1:822782/1‑251 (MQ=255)                                                                                                                                                                                                        a‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCt                                        >  1:208750/1‑251 (MQ=255)                                                                                                                                                                                                             ttCGCGGTAAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt                                     <  1:774079/250‑1 (MQ=255)                                                                                                                                                                                                               tggaggtgggggtgtgggaaaaaaCCAGTGAGGAGAATTTTTTTGCC‑ACCGGGGTGATTTTTT‑GTTTTTCCCGC‑CAGGAGAGATTCATCACTATGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAAGGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                                    <  2:749589/231‑1 (MQ=255)                                                                                                                                                                                                                  ggTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGc                                >  1:707232/1‑250 (MQ=255)                                                                                                                                                                                                                   gTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAAAACGGGGTTCTTTCGCCCGGCTGAAGCGCAACAGCCGCATTTCAGCGACCTGTTTGGGCa                               >  2:737934/1‑250 (MQ=255)                                                                                                                                                                                                                     gggggggggg‑TGAAAAGTCAGAGGTGAGAATTTGTTTGCCGGCCGGG‑GGATATTGT‑GCTGTTCCCGC‑CAGGAGAGATTCAGCACTACGGTCGTCCTCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                            <  2:441589/249‑1 (MQ=255)                                                                                                                                                                                                                     cAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCCCCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                            >  1:100365/1‑251 (MQ=255)                                                                                                                                                                                                                     cAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTAGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGAGCACCAGCCGCATTTCAGCGACCTGTTTGGGCGAAt                            >  2:862187/1‑251 (MQ=255)                                                                                                                                                                                                                      aGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGGGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATCTTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATc                           >  2:889129/1‑251 (MQ=255)                                                                                                                                                                                                                        gggttgg‑tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGT‑GGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCTCCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt                         <  1:307309/247‑1 (MQ=255)                                                                                                                                                                                                                         gggTGG‑TGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt                        >  1:372619/1‑251 (MQ=255)                                                                                                                                                                                                                           gtgg‑tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCACGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCCGCCGCATTTCAGCGACCTGTTTGGGAAAATCATTa                       >  2:969186/1‑250 (MQ=255)                                                                                                                                                                                                                             gg‑tgAAAAATCAGGGACGAGAATTTGTTTGCCGCCCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg                    <  1:324811/251‑1 (MQ=255)                                                                                                                                                                                                                              gggaaaaaaTCAGGGACGAAAATTTGTTTGCCGCCCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg                    <  1:163689/247‑1 (MQ=255)                                                                                                                                                                                                                              g‑tgAAAAATCAGGGACGAGAATTTGTTGGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc                   <  1:62768/251‑1 (MQ=255)                                                                                                                                                                                                                                tgAAAAATCAGGGACGCGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAAAACGGGGTTCTTTCGCCCGGATGAAGCGCAACAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc                  >  2:922921/1‑251 (MQ=255)                                                                                                                                                                                                                                 gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCcg                                                                                                                                          >  1:419026/1‑130 (MQ=255)                                                                                                                                                                                                                                 gAAAAAGCAGGGACGAGAATTGGTTTGCCGACCGGT‑TGATATTTG‑GCGGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCcg                                                                                                                                          <  2:419026/130‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaaaaCAGGGACAAGAATTTTTTTCCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCCCTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCg                 <  2:734419/250‑1 (MQ=255)                                                                                                                                                                                                                                  aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGG‑TGTTATTTT‑GTTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCgg                <  1:719777/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaTCAGGGCGGAAAATTTTTTTGCCGACCGGT‑TGTTTGTTT‑GTTGTCCCCGC‑CAGGAGAGATTCATCACTTCGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGc              <  2:329620/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTCAGGGACGCGAATTTGATTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGCGATTAATCACTACGGTCGTCATCCGGAGGCTCGAGA‑ATGGTAACACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGGTCTTTCGCCCGGATGCAGCGCACAAGCCTCCTTTCCGCGACCTGGTTGGACAAATCATTAAAGACGGGCa             >  2:110829/1‑250 (MQ=255)                                                                                                                                                                                                                                        cAGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTCTGGGCAAATCATTAACGCCGGGCAAgg          >  2:667915/1‑251 (MQ=255)                                                                                                                                                                                                                                         aGGGACGAGAATTTGTTTGCCGACCGGG‑TGATATTTT‑GTTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAggg         <  1:580851/251‑1 (MQ=255)                                                                                                                                                                                                                                               gagaATTTTTTTGCCGACCGGT‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAggg   <  1:112102/251‑1 (MQ=255)                                                                                                                                                                                                                                                agaATTTGTTTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGgc  >  1:750493/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       GATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCA‑CCATTCGCGGTCAGGGGGTGG‑TGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGG‑TGATATTTT‑GCTGTTCCCGC‑CAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGA‑ATGGTATCACC‑AGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGC  >  NC_000913/70335‑70822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑