Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,875,5310CT24.5% 80.0 / 16.4 53H32Y (CAT→TAT) ‡yidXputative lipoprotein YidX
Reads supporting (aligned to +/- strand):  ref base C (24/16);  new base T (13/0);  total (37/16)
Fisher's exact test for biased strand distribution p-value = 5.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTT  >  NC_000913/3875286‑3875773
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
atTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAAACTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGTATTTTTTAAAGCTGACGGTTTATTTCCACTGGCGCTGATGCTTTATGGCTGTATCGCGGATGCTCTGGTTTCCCAttcc                                                                                                                                                                                                                                               >  2:238606/1‑248 (MQ=255)
 ttgttgTACTACAATTTAGATCACAAAAAGAACAATGCATAAATCAATGACATGAGTCGGGCAGAAATCTGAAACGGGATATCAGGCGCTAAACAGGAGGGCAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTTTGCTCTGGTTTCCCATACCg                                                                                                                                                                                                                                              >  2:727483/1‑251 (MQ=255)
   gttgtACTAAAATTTAGATCACAAAAAGAAAAATGAATAAAAAAATGACATGCGTCGGGCAGAAATATGAAAAGGGATATTCGGCGCTAAACAGGAGGGAAAGAAGTGTATGCTTGTAACGGATTAGCTACTCGTTTAAAGGATGAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGACTGTTGATTTCCACTGGCGCTGATGCTTTCATGCTGTTTCTCGTATGCTCTGGTGTCCCATACCGct                                                                                                                                                                                                                                            <  2:525636/251‑2 (MQ=255)
   ggtgtACTACAATTTAGATAACAAAAAGAACAATTCATAAATAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCAGGAAGTTGAATTTTAAGTGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCa                                                                                                                                                                                                                                            <  2:175988/249‑1 (MQ=255)
     tgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATTCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCgaaa                                                                                                                                                                                                                                           >  1:25407/1‑247 (MQ=255)
           acaagttaaataacaAAAAGAACAATGCATAAAAAAAAGACATGCGTCGGGAAGAAAGCTGAAAAGAAATATCAGGCGGTAAACAGGAGGGAAAGAAGAGAATGCTTTCAACGGGTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTTAATTATAAGGGATTTTTTAAGTCTGCCGGTTTATTCCCTCTGGCGTTGATGCTTTCAGGCTGTATCTCTTAGGCTCTGGTTTCCCATACCGCAAAGGGTa                                                                                                                                                                                                                                     <  2:439848/238‑1 (MQ=255)
             aaTTTAGATCACAAAAAGAACAATGCCTAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTACAGGATTAATCATGAAGTTGAATTTTAATGGATTTTTTAATTCTACCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGAATGCTCTGGTTTCCCATACCGCAACGGGAAGtt                                                                                                                                                                                                                                  >  2:389963/1‑251 (MQ=255)
             aaTTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACAGCAACGGGTAGtt                                                                                                                                                                                                                                  >  1:177411/1‑251 (MQ=255)
                   gATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCACCGGCTTAGCTACTCGTTTAAGGGATTAATCATGAGGTTGAATTTTAAGGGTTTTTTTAAGGTTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGACTGCTCTGGTTTCCCTTACCGAAAAGGGTaattaagaa                                                                                                                                                                                                                             >  2:376323/1‑242 (MQ=255)
                                 aaaTGCATAAAAAAAAGACATTCGTCGGTCTGAAATCTGAAAAGGGATATCAAGCACTAAACAGGAGGGAAAGAAGTGTATGCTTACAACGGCTTAGCTACTCGTTTAAAGGATTAAACATGAAGTTGAATTATAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTTAGACTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTGCAGTAAAGTATCAATctcct                                                                                                                                                                                                              <  2:380665/250‑5 (MQ=255)
                                          aaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGacac                                                                                                                                                                                                      >  1:644598/1‑250 (MQ=255)
                                          aaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTTTCCCAGACAGCAAAATTTAGTTTAGGAAAGGATCACTCTCAGTCAGACAcc                                                                                                                                                                                                     >  1:516376/1‑251 (MQ=255)
                                                        ggcGGGCATAAATCTGAAAAGTGATATCAGGCTCTAAACAATAGGTAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATGAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGATTTATTCCCTCTGTCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATc                                                                                                                                                                                       <  1:558192/249‑1 (MQ=255)
                                                        gTCGGGCTGAAATCTGAAAAGGTATATCAGGCGCTAAACAGGAGTGAAAGAAGAATATACTTTCAACGGATTAGCTACTCGTTTAAAGGATTAATCATGAAGGTGAATTTTAAGGGATGTTTTAAGGCTGCCGGTTTATTCCCTCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTTCCATAACGCAAAGGGTAGTTCAGGAAAGTATTAATCGCAGTCAGTCACCATCACTGTGCTATc                                                                                                                                                                                       <  2:859194/251‑1 (MQ=255)
                                                              cAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGc                                                                                                                                                                                 >  1:204702/1‑251 (MQ=255)
                                                               aGAAATTTGAAAAGGGAAAACAGGCGCAAAACAGGAAGGAAAGAAGAATAAGCTTACAACGGCTTAGCTACTCGTTTAAAGGATTAAGCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCa                                                                                                                                                                                <  2:397352/251‑1 (MQ=255)
                                                                         aaaGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCGTAGCTACACGTTTAAAGGATTAAACATGAAGTTGAATATTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCGTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTa                                                                                                                                                                      <  2:263855/251‑1 (MQ=255)
                                                                           aGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTCCTTTACCACAAAGGGAAGTTCAGGAAAGTATCAATCTCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAt                                                                                                                                                                    >  1:452460/1‑251 (MQ=255)
                                                                                   aaGGCGATAAACAGGAGTGAAAGAAGAGTATTCTTTCAACGTATTAACTACACGATTAAAAGATGAATAATAAAGTTGAATTTTAAGGGATGTTTTAAGGCTGCCGGTTTATTCCCACAGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGTGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGTTCTATCGCAGGCAAAATATAGTAATGGAACaaa                                                                                                                                                            <  2:254144/250‑1 (MQ=255)
                                                                                    aGGCGCTAAACAGGAGGGAAAGAAGAGTATGGTTGCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaaaa                                                                                                                                                           <  2:6017/251‑1 (MQ=255)
                                                                                           aaaCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCCCTGGGCTATCGCAGGAAAAAGATAGTAATGGAACAAAAGGCTa                                                                                                                                                      >  2:532501/1‑249 (MQ=255)
                                                                                             aCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTTAATTTTAAGGGATTTTCTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTGCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                                                                                                                                  <  2:328651/251‑1 (MQ=255)
                                                                                             aCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGTAGTGCAGGAAAGTATCAATCTCAGACAGACACCATAACTAAGCTAACGCAGGCAAAAGATAGTAAGGGCACAAAAGGGTATGtt                                                                                                                                                  >  1:505273/1‑251 (MQ=255)
                                                                                               aggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTCTGCTTTACAAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGCACAAACGGCTCTGtttt                                                                                                                                                >  1:427628/1‑251 (MQ=255)
                                                                                                ggaggGAAATAAGAGTATTCTTTCAACGTCTTAGCTCTTCATTTAAAGGATTAAACATGAAGTTGAATTTTAAGGTATTTTTTAAGGCGTACGGTTTATTCCGACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGttttt                                                                                                                                               <  1:211605/251‑1 (MQ=255)
                                                                                                 gaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTg                                                                                                                                              >  2:690594/1‑251 (MQ=255)
                                                                                                      aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTTAAAGAAAATAGAAGATCAGGAAAGTATCAATCGAAGTCAGACACCATCACTGGGATATCGCAGGCAAAAGATAGTAATAGAACAACAGGCAATGTTTTTGTAggg                                                                                                                                         >  1:206555/1‑251 (MQ=255)
                                                                                                        agaagaGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAGCAAAGAGTAGTTCTGGAAAGTATCAATCGCAGTCAGACACCATAACTGGGCTATCGCAGGCAAAAGATAGAAATGGAACAAACGGCTATGTTTTTGTAgggg                                                                                                                                        >  1:874505/1‑250 (MQ=255)
                                                                                                           agagTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTTTGCTATACAACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACAATCACTGGGCTATCGCAGGCAAAAGATAGGAATGGAACACAAGGCTATGTTTTTGTAGGGGAATc                                                                                                                                    >  1:961036/1‑251 (MQ=255)
                                                                                                                        aaCGGCTTAGCTACTCGTTTAAAGTATTAATTATGAAGTTGAATTTTAAGGGATTATTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCtt                                                                                                                       <  1:210078/251‑1 (MQ=255)
                                                                                                                                aGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTg                                                                                                                >  1:649200/1‑250 (MQ=255)
                                                                                                                                aGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGATAGTTCAGGAAAGTATCAATCGCAGGCAGACACCATAACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTAGGTTTTTGTAGGGGTATCGCTGGATTACCTTATCACTgc                                                                                                               >  2:738354/1‑250 (MQ=255)
                                                                                                                                        gTTTAACGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCAATGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCGCTGGTTTCCCATACAGCAAAGAGTAGGTCAGGAAAGTATCAATAGCAGTCAGACACCATCACTCGGATATCGCAAGCCAAAGATAGAAACGGAACAAAAGGCTATGTTTTTGTAGGAGAATCGCTGGATTACCTTATCACGTATGGTgacg                                                                                                       >  2:526061/1‑248 (MQ=255)
                                                                                                                                             aaGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGATAGATAAGGAAAGAGTCAATCGCAGTTAGACACCATCACTGGGCTATCGCCGGCAACAGATAGTAATGGAACAACAGGCTATGTGTTTGTAGGGGAACCGCTGGATTACCttcacgataatggtgccggtga                                                                                                   >  1:292704/1‑230 (MQ=255)
                                                                                                                                               ggATTAATCATGAAGTTGCATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTTACAACAAAGGGTAGTTCAGGACAGTATCAATCACAGCCAGACACCATCACTGGGCTATAGAAGGCAAAAGCTAGTAATGGAACAAAAGACTTTGTTTTTGTAGTCGAATCACAGGATTACCTTATCACTGATGGTGACGGTGACAt                                                                                                >  1:630483/1‑251 (MQ=255)
                                                                                                                                                     aTCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGAAGATCAGGAAAGTATCAATCGCAGTCAGACAACATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATAGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTa                                                                                           >  1:665392/1‑250 (MQ=255)
                                                                                                                                                        aTGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATAGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGCATCGCTGGATTCCCTTATCACTGATGGTGCCGATGACATCTTTAAgct                                                                                       >  1:443850/1‑249 (MQ=255)
                                                                                                                                                        aTGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCTTCTCCTTTACCAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCACAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTa                                                                                           >  1:192568/1‑247 (MQ=255)
                                                                                                                                                                    tttAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAATGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGGTAAGATGCTCAATGATcc                                                                           >  1:631865/1‑251 (MQ=255)
                                                                                                                                                                        aGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAGGTAGTTCAGGAAAGTATAACTCGCAATCAGACACCATAACTGGGCTATCGCACGCAAAAGATAGTAATGGAACAACAGACTATGTTTTTGCAGGGTAAACGCTGGATTAACTTAACACTGAAGGTGACGATCACATAGTTAAGATGCTAAATGATACAGc                                                                        >  1:964487/1‑250 (MQ=255)
                                                                                                                                                                          ggATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACCGCAAAGGGTCGTTCAGGCAAGTATCAATCGCAGTAAGACACCATCACTGAGCTATCGCAGGCACAAGATAGTAATGGACCAGAAGGCTCTGTTTTTGTAGGGGAATCGCTGAATTACCTTATCACTAATGGAGCCGATGACATCGTTCAGATGCTCAATgcacaagcatt                                                                     >  2:319227/1‑241 (MQ=255)
                                                                                                                                                                          ggATTGTTTAAGGCTGCCGGTTTATTTCCACGGGCACTGATGCTTTCAGGCTGGATACCGTATGCACTGATTTCCCATTGCGCAAAGGGTAGTACAGGAAAGTATCAATCACAGTCAGACCCCATCACTGGTCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGTATCGCTTTATTACCTTATCACTGATGGTGCCTATGCCATCGTTAAGATGCTCAATGATCCAGCAcg                                                                     <  2:540651/251‑2 (MQ=255)
                                                                                                                                                                             ttttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCTTCTGCCTGACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTa                                                                   >  1:65799/1‑250 (MQ=255)
                                                                                                                                                                                       cTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTTCCCCTACCGCAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCCGGCAAACGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGCGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAat                                                        >  1:711601/1‑251 (MQ=255)
                                                                                                                                                                                         gCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTCCTTTACAAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAACAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGATCAATGATCCAGAACTTAACCGGCACAatat                                                      >  1:5502/1‑251 (MQ=255)
                                                                                                                                                                                                tATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGt                                                <  1:8700/250‑1 (MQ=255)
                                                                                                                                                                                                tATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGAATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGAATCAATCACAGTCAGCCACCATCACTGGGCTATCGCAGGCAAAAGATAGCAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATAACTGATGGTGCCGATGAAATCGTTACGATGCTCAATGCTCCAACACTTATCCGGCACAATCTTCAGGtt                                               >  2:926221/1‑251 (MQ=255)
                                                                                                                                                                                                   tCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTTTCCTATACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGcc                                            >  1:434171/1‑251 (MQ=255)
                                                                                                                                                                                                       aCTGGCGCTGATGCTTTCAGGCTGTATCTTGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCGTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATg                                        <  1:690594/251‑1 (MQ=255)
                                                                                                                                                                                                             gcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCCAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCa                                   >  2:105449/1‑250 (MQ=255)
                                                                                                                                                                                                                                  ctcGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCgggg              >  2:698422/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGaaa           >  2:193528/1‑250 (MQ=255)
                                                                                                                                                                                                                                             tGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGTCGATGACATCGTTAAGATGCTCAATGATCCAGCCCTTAACCGGCACAATATTCAGGTTGCCGATGACGCACGATTTGTTTTAAATGCGGGGACAAAGAAAttt  >  2:467584/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
ATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTT  >  NC_000913/3875286‑3875773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑