Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,875,521	0	T	C	28.3%	72.3 / 22.0	55	A28A (GCT→GCC)	yidX	putative lipoprotein YidX
Reads supporting (aligned to +/- strand):  ref base T (21/17);  new base C (15/0);  total (37/18)
Fisher's exact test for biased strand distribution p-value = 1.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAA  >  NC_000913/3875277‑3875764                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    cAAACCTATATTGTTGTACTTCAATTTAGATCAAAAAAAGAAACATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAATGGGGATAACAGGCGCTAAACAGGAGGGAAAGAAGAGTCTGCAGTCAACGGTTTAGCTACTCGTTTAAAGGATTATTCATGATGTCTAATGTTAAGGGATTTTTTAAGGCTGACGGTTTATGCCCACTGGCGCTTATGCTTTCAGGGTGTATCTCGTATGCTCTGGtt                                                                                                                                                                                                                                               <  2:403673/251‑1 (MQ=255)          atTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAAACTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGTATTTTTTAAAGCTGACGGTTTATTTCCACTGGCGCTGATGCTTTATGGCTGTATCGCGGATGCTCTGGTTTCCCAttcc                                                                                                                                                                                                                                      >  2:238606/1‑248 (MQ=255)           ttgttgTACTACAATTTAGATCACAAAAAGAACAATGCATAAATCAATGACATGAGTCGGGCAGAAATCTGAAACGGGATATCAGGCGCTAAACAGGAGGGCAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTTTGCTCTGGTTTCCCATACCg                                                                                                                                                                                                                                     >  2:727483/1‑251 (MQ=255)             gttgtACTAAAATTTAGATCACAAAAAGAAAAATGAATAAAAAAATGACATGCGTCGGGCAGAAATATGAAAAGGGATATTCGGCGCTAAACAGGAGGGAAAGAAGTGTATGCTTGTAACGGATTAGCTACTCGTTTAAAGGATGAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGACTGTTGATTTCCACTGGCGCTGATGCTTTCATGCTGTTTCTCGTATGCTCTGGTGTCCCATACCGct                                                                                                                                                                                                                                   <  2:525636/251‑2 (MQ=255)             ggtgtACTACAATTTAGATAACAAAAAGAACAATTCATAAATAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCAGGAAGTTGAATTTTAAGTGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCa                                                                                                                                                                                                                                   <  2:175988/249‑1 (MQ=255)               tgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATTCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCgaaa                                                                                                                                                                                                                                  >  1:25407/1‑247 (MQ=255)                     acaagttaaataacaAAAAGAACAATGCATAAAAAAAAGACATGCGTCGGGAAGAAAGCTGAAAAGAAATATCAGGCGGTAAACAGGAGGGAAAGAAGAGAATGCTTTCAACGGGTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTTAATTATAAGGGATTTTTTAAGTCTGCCGGTTTATTCCCTCTGGCGTTGATGCTTTCAGGCTGTATCTCTTAGGCTCTGGTTTCCCATACCGCAAAGGGTa                                                                                                                                                                                                                            <  2:439848/238‑1 (MQ=255)                       aaTTTAGATCACAAAAAGAACAATGCCTAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTACAGGATTAATCATGAAGTTGAATTTTAATGGATTTTTTAATTCTACCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGAATGCTCTGGTTTCCCATACCGCAACGGGAAGtt                                                                                                                                                                                                                         >  2:389963/1‑251 (MQ=255)                       aaTTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACAGCAACGGGTAGtt                                                                                                                                                                                                                         >  1:177411/1‑251 (MQ=255)                             gATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCACCGGCTTAGCTACTCGTTTAAGGGATTAATCATGAGGTTGAATTTTAAGGGTTTTTTTAAGGTTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGACTGCTCTGGTTTCCCTTACCGAAAAGGGTaattaagaa                                                                                                                                                                                                                    >  2:376323/1‑242 (MQ=255)                                           aaaTGCATAAAAAAAAGACATTCGTCGGTCTGAAATCTGAAAAGGGATATCAAGCACTAAACAGGAGGGAAAGAAGTGTATGCTTACAACGGCTTAGCTACTCGTTTAAAGGATTAAACATGAAGTTGAATTATAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTTAGACTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTGCAGTAAAGTATCAATctcct                                                                                                                                                                                                     <  2:380665/250‑5 (MQ=255)                                                    aaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGacac                                                                                                                                                                                             >  1:644598/1‑250 (MQ=255)                                                    aaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTTTCCCAGACAGCAAAATTTAGTTTAGGAAAGGATCACTCTCAGTCAGACAcc                                                                                                                                                                                            >  1:516376/1‑251 (MQ=255)                                                                  ggcGGGCATAAATCTGAAAAGTGATATCAGGCTCTAAACAATAGGTAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATGAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGATTTATTCCCTCTGTCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATc                                                                                                                                                                              <  1:558192/249‑1 (MQ=255)                                                                  gTCGGGCTGAAATCTGAAAAGGTATATCAGGCGCTAAACAGGAGTGAAAGAAGAATATACTTTCAACGGATTAGCTACTCGTTTAAAGGATTAATCATGAAGGTGAATTTTAAGGGATGTTTTAAGGCTGCCGGTTTATTCCCTCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTTCCATAACGCAAAGGGTAGTTCAGGAAAGTATTAATCGCAGTCAGTCACCATCACTGTGCTATc                                                                                                                                                                              <  2:859194/251‑1 (MQ=255)                                                                        cAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGc                                                                                                                                                                        >  1:204702/1‑251 (MQ=255)                                                                         aGAAATTTGAAAAGGGAAAACAGGCGCAAAACAGGAAGGAAAGAAGAATAAGCTTACAACGGCTTAGCTACTCGTTTAAAGGATTAAGCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCa                                                                                                                                                                       <  2:397352/251‑1 (MQ=255)                                                                                   aaaGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCGTAGCTACACGTTTAAAGGATTAAACATGAAGTTGAATATTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCGTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTa                                                                                                                                                             <  2:263855/251‑1 (MQ=255)                                                                                     aGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTCCTTTACCACAAAGGGAAGTTCAGGAAAGTATCAATCTCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAt                                                                                                                                                           >  1:452460/1‑251 (MQ=255)                                                                                             aaGGCGATAAACAGGAGTGAAAGAAGAGTATTCTTTCAACGTATTAACTACACGATTAAAAGATGAATAATAAAGTTGAATTTTAAGGGATGTTTTAAGGCTGCCGGTTTATTCCCACAGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGTGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGTTCTATCGCAGGCAAAATATAGTAATGGAACaaa                                                                                                                                                   <  2:254144/250‑1 (MQ=255)                                                                                              aGGCGCTAAACAGGAGGGAAAGAAGAGTATGGTTGCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaaaa                                                                                                                                                  <  2:6017/251‑1 (MQ=255)                                                                                                     aaaCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCCCTGGGCTATCGCAGGAAAAAGATAGTAATGGAACAAAAGGCTa                                                                                                                                             >  2:532501/1‑249 (MQ=255)                                                                                                       aCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTTAATTTTAAGGGATTTTCTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTGCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtt                                                                                                                                         <  2:328651/251‑1 (MQ=255)                                                                                                       aCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGTAGTGCAGGAAAGTATCAATCTCAGACAGACACCATAACTAAGCTAACGCAGGCAAAAGATAGTAAGGGCACAAAAGGGTATGtt                                                                                                                                         >  1:505273/1‑251 (MQ=255)                                                                                                         aggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGt                                                                                                                                                                                                                                                >  1:147899/1‑146 (MQ=255)                                                                                                         aggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTCTGCTTTACAAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGCACAAACGGCTCTGtttt                                                                                                                                       >  1:427628/1‑251 (MQ=255)                                                                                                         aggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCGACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTGTGAAGTCTGCCGGTTTATTCCCACTGGCGCTGAGGCTTTCAGGCTGTATCTCGTATGCTCTGGt                                                                                                                                                                                                                                                <  2:147899/146‑1 (MQ=255)                                                                                                          ggaggGAAATAAGAGTATTCTTTCAACGTCTTAGCTCTTCATTTAAAGGATTAAACATGAAGTTGAATTTTAAGGTATTTTTTAAGGCGTACGGTTTATTCCGACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGttttt                                                                                                                                      <  1:211605/251‑1 (MQ=255)                                                                                                           gaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTg                                                                                                                                     >  2:690594/1‑251 (MQ=255)                                                                                                                aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTTAAAGAAAATAGAAGATCAGGAAAGTATCAATCGAAGTCAGACACCATCACTGGGATATCGCAGGCAAAAGATAGTAATAGAACAACAGGCAATGTTTTTGTAggg                                                                                                                                >  1:206555/1‑251 (MQ=255)                                                                                                                  agaagaGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAGCAAAGAGTAGTTCTGGAAAGTATCAATCGCAGTCAGACACCATAACTGGGCTATCGCAGGCAAAAGATAGAAATGGAACAAACGGCTATGTTTTTGTAgggg                                                                                                                               >  1:874505/1‑250 (MQ=255)                                                                                                                     agagTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTTTGCTATACAACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACAATCACTGGGCTATCGCAGGCAAAAGATAGGAATGGAACACAAGGCTATGTTTTTGTAGGGGAATc                                                                                                                           >  1:961036/1‑251 (MQ=255)                                                                                                                                  aaCGGCTTAGCTACTCGTTTAAAGTATTAATTATGAAGTTGAATTTTAAGGGATTATTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCtt                                                                                                              <  1:210078/251‑1 (MQ=255)                                                                                                                                          aGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTg                                                                                                       >  1:649200/1‑250 (MQ=255)                                                                                                                                          aGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGATAGTTCAGGAAAGTATCAATCGCAGGCAGACACCATAACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTAGGTTTTTGTAGGGGTATCGCTGGATTACCTTATCACTgc                                                                                                      >  2:738354/1‑250 (MQ=255)                                                                                                                                                  gTTTAACGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCAATGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCGCTGGTTTCCCATACAGCAAAGAGTAGGTCAGGAAAGTATCAATAGCAGTCAGACACCATCACTCGGATATCGCAAGCCAAAGATAGAAACGGAACAAAAGGCTATGTTTTTGTAGGAGAATCGCTGGATTACCTTATCACGTATGGTgacg                                                                                              >  2:526061/1‑248 (MQ=255)                                                                                                                                                       aaGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGATAGATAAGGAAAGAGTCAATCGCAGTTAGACACCATCACTGGGCTATCGCCGGCAACAGATAGTAATGGAACAACAGGCTATGTGTTTGTAGGGGAACCGCTGGATTACCttcacgataatggtgccggtga                                                                                          >  1:292704/1‑230 (MQ=255)                                                                                                                                                         ggATTAATCATGAAGTTGCATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTTACAACAAAGGGTAGTTCAGGACAGTATCAATCACAGCCAGACACCATCACTGGGCTATAGAAGGCAAAAGCTAGTAATGGAACAAAAGACTTTGTTTTTGTAGTCGAATCACAGGATTACCTTATCACTGATGGTGACGGTGACAt                                                                                       >  1:630483/1‑251 (MQ=255)                                                                                                                                                               aTCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGAAGATCAGGAAAGTATCAATCGCAGTCAGACAACATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATAGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTa                                                                                  >  1:665392/1‑250 (MQ=255)                                                                                                                                                                  aTGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATAGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGCATCGCTGGATTCCCTTATCACTGATGGTGCCGATGACATCTTTAAgct                                                                              >  1:443850/1‑249 (MQ=255)                                                                                                                                                                  aTGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCTTCTCCTTTACCAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCACAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTa                                                                                  >  1:192568/1‑247 (MQ=255)                                                                                                                                                                              tttAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAATGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGGTAAGATGCTCAATGATcc                                                                  >  1:631865/1‑251 (MQ=255)                                                                                                                                                                                  aGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAGGTAGTTCAGGAAAGTATAACTCGCAATCAGACACCATAACTGGGCTATCGCACGCAAAAGATAGTAATGGAACAACAGACTATGTTTTTGCAGGGTAAACGCTGGATTAACTTAACACTGAAGGTGACGATCACATAGTTAAGATGCTAAATGATACAGc                                                               >  1:964487/1‑250 (MQ=255)                                                                                                                                                                                    ggATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACCGCAAAGGGTCGTTCAGGCAAGTATCAATCGCAGTAAGACACCATCACTGAGCTATCGCAGGCACAAGATAGTAATGGACCAGAAGGCTCTGTTTTTGTAGGGGAATCGCTGAATTACCTTATCACTAATGGAGCCGATGACATCGTTCAGATGCTCAATgcacaagcatt                                                            >  2:319227/1‑241 (MQ=255)                                                                                                                                                                                    ggATTGTTTAAGGCTGCCGGTTTATTTCCACGGGCACTGATGCTTTCAGGCTGGATACCGTATGCACTGATTTCCCATTGCGCAAAGGGTAGTACAGGAAAGTATCAATCACAGTCAGACCCCATCACTGGTCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGTATCGCTTTATTACCTTATCACTGATGGTGCCTATGCCATCGTTAAGATGCTCAATGATCCAGCAcg                                                            <  2:540651/251‑2 (MQ=255)                                                                                                                                                                                       ttttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCTTCTGCCTGACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTa                                                          >  1:65799/1‑250 (MQ=255)                                                                                                                                                                                                 cTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTTCCCCTACCGCAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCCGGCAAACGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGCGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAat                                               >  1:711601/1‑251 (MQ=255)                                                                                                                                                                                                   gCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTCCTTTACAAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAACAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGATCAATGATCCAGAACTTAACCGGCACAatat                                             >  1:5502/1‑251 (MQ=255)                                                                                                                                                                                                          tATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGt                                       <  1:8700/250‑1 (MQ=255)                                                                                                                                                                                                          tATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGAATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGAATCAATCACAGTCAGCCACCATCACTGGGCTATCGCAGGCAAAAGATAGCAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATAACTGATGGTGCCGATGAAATCGTTACGATGCTCAATGCTCCAACACTTATCCGGCACAATCTTCAGGtt                                      >  2:926221/1‑251 (MQ=255)                                                                                                                                                                                                             tCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTTTCCTATACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGcc                                   >  1:434171/1‑251 (MQ=255)                                                                                                                                                                                                                 aCTGGCGCTGATGCTTTCAGGCTGTATCTTGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCGTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATg                               <  1:690594/251‑1 (MQ=255)                                                                                                                                                                                                                       gcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCCAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCa                          >  2:105449/1‑250 (MQ=255)                                                                                                                                                                                                                                            ctcGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCgggg     >  2:698422/1‑250 (MQ=255)                                                                                                                                                                                                                                               gTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGaaa  >  2:193528/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAA  >  NC_000913/3875277‑3875764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑