Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,883	1	.	C	91.3%	243.2 / 16.2	92	coding (667/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base C (54/30);  total (58/34)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑C‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGC  >  NC_000913/3815634‑3816132                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                            aTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑cc‑c‑cc                                                                                                                                                                                                                                                             >  1:929993/1‑250 (MQ=255)    gcgcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGTC‑TTTTGCGCCCGGATTGCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATGCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTTCAACGGATTCGAT‑T‑cc‑c‑cctcg                                                                                                                                                                                                                                                          <  2:119931/251‑5 (MQ=255)      gcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑Cggg                                                                                                                                                                                                                                                       >  1:286444/1‑251 (MQ=255)       cccGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTGGATTCGAT‑T‑CC‑C‑CCT‑CGGGc                                                                                                                                                                                                                                                      >  2:770519/1‑251 (MQ=255)          ggTATTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAAATCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTCCAATGGTTTCGAT‑T‑CC‑C‑CCT‑CGGGcccg                                                                                                                                                                                                                                                   >  2:551754/1‑249 (MQ=255)           gtgatAAACAGCCGGGGGTGGAAGAAATAGGGGCATTGTG‑GCCCGGATTTCC‑GCGTAAACCCGCCAACCTTTAACACCTGCTTGTGAAGCTCAACTGCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTGCTTTTCTACAGACAAAAAAA‑GGCGACTCATCAGGCGCCGGAAAAATCAGCTGGCCAGCGCCGCTTTCTGCGGCGCTACAAGGGATTCGAT‑G‑CC‑CAC‑T‑AGGGCCagag                                                                                                                                                                                                                                                 <  2:829243/247‑1 (MQ=255)             aTTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAAAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGc                                                                                                                                                                                                                                                >  1:74758/1‑251 (MQ=255)                 aaCAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAACCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACTGACAAAAAAAATGCGGCTCATCAGGCGCCTTAAAAATCAGTTTGCCAGCGCCGTCTTCTGCGTCGCTACAATGTATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGATCcaac                                                                                                                                                                                                                                            >  1:7772/1‑251 (MQ=255)                     gCCCGGAGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATCTCA‑GAGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGATCCTCATCTTACTTTTCTACAGATAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGa                                                                                                                                                                                                                                        <  1:546598/251‑1 (MQ=255)                      cccGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAATTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAAAATGGATTCGAT‑TACC‑C‑C‑T‑CGGGCCAGAGCCAACAAgtt                                                                                                                                                                                                                                       >  2:296206/1‑249 (MQ=255)                       ccGGCGTTGAATAAATAGGGGC‑TTTTCCGCCCGGATTTCA‑GCTTAAACTCGCCAAACTTTAACATCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATATTTCTTTTCTACAGACAAAAAAAAGGCGCCGCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATg                                                                                                                                                                                                                                      <  2:534908/251‑1 (MQ=255)                       ccGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATg                                                                                                                                                                                                                                      >  1:645699/1‑251 (MQ=255)                        cGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATg                                                                                                                                                                                                                                      >  1:259714/1‑250 (MQ=255)                          gCGTTGAAGAAATAGGGGC‑TTTTGCGCTCTGATTACA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAATCGCAAATTCAATAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGa                                                                                                                                                                                                                                     <  1:13826/249‑1 (MQ=255)                           cGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGt                                                                                                                                                                                                                                   >  1:880423/1‑250 (MQ=255)                             ttGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCAAGCGCCTCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑CAC‑T‑CGGGCCAGAGCCAACAAGATGAGTAGc                                                                                                                                                                                                                                >  2:331786/1‑251 (MQ=255)                               aaaaaaaTAGGTGC‑TTTTGCGCCCGGATT‑CATGCGGAAAATCGCTAAGCCTTAACTCCTGCTTGCTAATCGCAAATTCAATAAACTGGCGCTGATATGGATTCATTCCTTCGCTCCTCATCTTACTTTGCTACTGACAATAAAAAGGCGGCTCATCTTTCTTCTTAAAACTCAGGTTGCCCACGTCGCCTTCTGCGTCGGTGCAAAGGAGTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGAGGAGTAGctc                                                                                                                                                                                                                              <  2:619836/247‑1 (MQ=255)                                aagaaATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAGCAGTTTGCCAGCGCCGCCTCCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGctct                                                                                                                                                                                                                             <  1:178555/251‑1 (MQ=255)                                       aGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                                                                                                                                                                                                       <  2:138391/250‑1 (MQ=255)                                               tttGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CCAC‑C‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                                                                                                                                                                                                 <  1:701560/249‑1 (MQ=255)                                                    gcCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCATTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                                                                                                                                          <  1:644853/251‑1 (MQ=255)                                                        ggATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                                                                                                                                                      >  1:624619/1‑251 (MQ=255)                                                             tCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCCCGCCTTCTGCCg                                                                                                                                                                                                 >  2:565773/1‑251 (MQ=255)                                                              tcctcGTAAACTCACCAAACGTTAGCTCCTGCTTTCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTCAGTCCTTCCCTCCTCATCTTACTTTTCGACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAGCATTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGGAGCTCTTCATGGGTGAACGGCGCGCCTTCTGcc                                                                                                                                                                                                  <  2:36674/246‑1 (MQ=255)                                                              atagcggaaatacGCCAAACTGTAATTCCTGTTTGCTAAGCGCAAATCAAAAAAACTGGCGTTGATATGGTTTCATGCCTTCGCTCATCATCTTACTTTTATACAGACAAAAAAAAGGCGACTCACCAGTCGCCTGAAAAACCAGTTTGCCAGCGCCGCCTTATGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑C‑TTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTAGCCTCCTgtcg                                                                                                                                                                                                 <  2:593/239‑4 (MQ=255)                                                                   gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                                                                                                                                            <  1:542185/251‑1 (MQ=255)                                                                     aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                                                                                                                                          <  1:662234/251‑1 (MQ=255)                                                                               aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                                                                                                                                                >  1:81995/1‑251 (MQ=255)                                                                                     aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                                                                                                                                           <  2:337733/250‑1 (MQ=255)                                                                                                 ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                                                                                                                                              >  1:741561/1‑251 (MQ=255)                                                                                                   aaGCGCAAATTCAATAAACTGGCGCTGAGATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAACAAAGGCGACTCATCAGTCGCCTTACAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑CAC‑T‑CGGGCCAGAGCCAACAAGAGGAGTAGCTCTTCATGGGAGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGAATCTCCCGTCTTCGGtcat                                                                                                                                                            >  2:36362/1‑251 (MQ=255)                                                                                                         aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCCCTAc                                                                                                                                                      >  2:906677/1‑251 (MQ=255)                                                                                                          aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCGACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAATACg                                                                                                                                                     <  2:564435/251‑1 (MQ=255)                                                                                                          aaTTCAATAAACTGGCGCTGATATGGTTTCATGCATTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                                                                                                                                                     >  1:262834/1‑251 (MQ=255)                                                                                                                aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCGTCAGTCGCCTTAAAAATCAGTTTGCCAACGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGAGCCAGAGCCAACAAGATGAGTAGCTCTTCATTGGTGAACGGCTCGCCATCTGCCGTCCCCTGCACGTCAATGCTGCGCCCGTATTCGGTCATCACTACGTTCAtt                                                                                                                                               >  2:169717/1‑250 (MQ=255)                                                                                                                   aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                                                                                                                                            <  1:279183/251‑1 (MQ=255)                                                                                                                       ggCGCTGATATGGATTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtc                                                                                                                                         <  2:259714/250‑1 (MQ=255)                                                                                                                       gccgcTGATATGGTTTCATGCCCTCGCTCCTCATCTTACGTTTCTACAGAAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                                                                                                                                          <  2:20793/247‑1 (MQ=255)                                                                                                                         cgcTGATATGGTGTCATGCCTTCGCTCCTCAGCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGACTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATTGATTCGAT‑T‑CC‑C‑CCG‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct                                                                                                                                      <  1:360713/251‑1 (MQ=255)                                                                                                                          gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGCTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTg                                                                                                                                     >  1:38037/1‑251 (MQ=255)                                                                                                                             gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCgg                                                                                                                                  >  1:472615/1‑251 (MQ=255)                                                                                                                               tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGc                                                                                                                                 >  1:856207/1‑250 (MQ=255)                                                                                                                               tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAACGCCGCCTTCTGCGTCGCTACAATGGCTTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGGGCCAACAAGATGAGTAGCTATTCTTGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGc                                                                                                                                 >  1:871123/1‑250 (MQ=255)                                                                                                                                     ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt                                                                                                                          >  1:481077/1‑251 (MQ=255)                                                                                                                                     ttCATGACTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGCT‑T‑CC‑C‑CCT‑CGGGCCAGCGCCAACAAGATGAGTAACTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCTGTATTCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt                                                                                                                          >  2:595447/1‑251 (MQ=255)                                                                                                                                      tCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt                                                                                                                          <  1:951730/250‑1 (MQ=255)                                                                                                                                       cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGCTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTc                                                                                                                        >  1:866067/1‑251 (MQ=255)                                                                                                                                       cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCCCCTTTAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTAGCTAAAATGGCTTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGCGCAAACAAGATGAGTAACTATACATGGGGAAACGGCTCGCCTTCTACCGACCCCTGAGATTGAATGATCGGCCCGTCGTCGGACATAACTACGTTCCTGTCGGTCCGTGCGTCAGAGTATTc                                                                                                                        >  2:591019/1‑251 (MQ=255)                                                                                                                                       cATGCCTTCGCTCCTCATCTTACTTTCCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCTTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTc                                                                                                                        <  1:416307/251‑1 (MQ=255)                                                                                                                                              tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGt                                                                                                                   <  2:645699/249‑1 (MQ=255)                                                                                                                                                 ctcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCACTGATGCGCCCGTCTTCGGTCCTCCCTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                                              >  2:452896/1‑251 (MQ=255)                                                                                                                                                  tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCCTGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                                              >  2:767026/1‑250 (MQ=255)                                                                                                                                                      cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCTGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTGATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATc                                                                                                         <  1:441849/251‑1 (MQ=255)                                                                                                                                                       aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                                              >  1:919530/1‑245 (MQ=255)                                                                                                                                                            aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAAc                                                                                                   >  1:706015/1‑251 (MQ=255)                                                                                                                                                             attttCTACAGACAAAAAAAAAGCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCCTGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAAATCGCAAAcc                                                                                                  >  2:644188/2‑251 (MQ=255)                                                                                                                                                                   tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCg                                                                                            >  1:410019/1‑251 (MQ=255)                                                                                                                                                                     cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTACGcc                                                                                          >  1:689106/1‑251 (MQ=255)                                                                                                                                                                            aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCACAGTATTCCAGATCGCAACCCGCTTCGCCGTTcccc                                                                                   >  2:232309/1‑248 (MQ=255)                                                                                                                                                                                    cGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGACGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCCCAATTCCGAc                                                                           >  2:154215/1‑251 (MQ=255)                                                                                                                                                                                      aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCg                                                                                            >  2:121299/1‑232 (MQ=255)                                                                                                                                                                                      aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc                                                                           >  1:802314/1‑249 (MQ=255)                                                                                                                                                                                      aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATGCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCg                                                                                            <  1:121299/232‑1 (MQ=255)                                                                                                                                                                                       cgcatcaGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑Ga                                                                       <  2:379587/249‑1 (MQ=255)                                                                                                                                                                                        tcatcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGACTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGCGGCTTCAACGTATTCCAGATCGCAAACCGCTTCCCCGTTCACAATTCCAACA‑Ga                                                                       >  2:894185/1‑250 (MQ=255)                                                                                                                                                                                         catcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGAGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACAGCTTCGACGTTCACAATTCCGCCA‑Gaa                                                                      >  2:4149/1‑250 (MQ=255)                                                                                                                                                                                              gTCGCCTTAAAATTCAGGTTGCGAACGCCGCCTTCCGCGTCGCTACCGTGGATTAACT‑T‑CC‑CAC‑T‑CGGCCAAGAGCCAACAAGATGGGTAGCTCTCCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCCGTCTTCGGCCATCACTACGTTCCTGTCGGACTCTGCGGCAGAGCCTTGAACGTCTTGCAGATCGCAAAACGCTTCGCCGTTCACCATTCCGACA‑GCCACTGCg                                                                >  2:81207/1‑251 (MQ=255)                                                                                                                                                                                              gTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGCCGCTACACTGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACCAGATGAGCAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCATCCCCTGCACTTCCATGATGCGCCCGTCTTCGGTCATCACAACGTTCATGTCGGTCTCTGCGGCACAGGCTTCAACGTATTCCAGATCGCCAACCGCTTCGCCGTTCACAATTCCgtaatcaccagcg                                                                 >  2:81825/1‑239 (MQ=255)                                                                                                                                                                                              gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGGTTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCACCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGATCACAATTCCGACC‑GAAACtacg                                                                >  1:627828/1‑248 (MQ=255)                                                                                                                                                                                                ccgcgtAAAAAGCAGTTTGCCAGCGCCGCCTTCTGCATCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGGGCCAACAAGATGAGTATCTCTTCAGGGGTAAACGGCTCTCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGATCCTCACTACGTTCATGTCGGTCTCTGCGGCAGGGTCTTCAACGTGTTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGc                                                              <  1:880581/246‑1 (MQ=255)                                                                                                                                                                                                    ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTAcc                                                          >  1:535200/1‑251 (MQ=255)                                                                                                                                                                                                     tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTAc                                                           <  1:672917/249‑1 (MQ=255)                                                                                                                                                                                                       aaaaTCAGTTTGCCAGCGCTGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATc                                                       <  1:528696/251‑1 (MQ=255)                                                                                                                                                                                                          aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCt                                                    >  2:315006/1‑251 (MQ=255)                                                                                                                                                                                                              gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCACTACGTTCATGTCGGCCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GCAACTGCGGCCACCATCCCTTTcc                                                >  2:43993/1‑250 (MQ=255)                                                                                                                                                                                                              gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTc                                                 >  1:688877/1‑250 (MQ=255)                                                                                                                                                                                                                    cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGAt                                          >  1:383838/1‑251 (MQ=255)                                                                                                                                                                                                                      gctccgcctctcggggcGCTATAATGGCTCCGAT‑T‑CC‑C‑CCA‑CGGGCCTGAGCCAACAAGATGCGTAGCTCTTCAGGGGTGAACGGCTCGCCTTCTGCCGTTCCCTGCACTTAAATGATGCGCCCGCCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAACCCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTCCCATCCCTTTCATCGGAt                                          <  2:818127/233‑1 (MQ=255)                                                                                                                                                                                                                        gccgccTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGt                                      >  1:757135/1‑251 (MQ=255)                                                                                                                                                                                                                         ccgccTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAAATCACAAACCGCTTCGCCGTTCACAATTCCG‑CACGAAACTGCGGCCACCATCCCTTTCATCGGATTTGtt                                     >  2:754457/1‑251 (MQ=255)                                                                                                                                                                                                                           gcccgtttcGTCGCTACAATGGATTCAATGC‑CC‑C‑C‑C‑CGGGCCTGAGCCAACAATATGAGTAGCTCGCTATTGGTGACCGTCTTGCCTTCTGCCGTCCCTAGCACTTCAATGATGCGCCCGTCTTCGGTCAGCACTACTGTCATGTCGGTCGCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTGCTGCGTTCACAATTCCGACA‑GAAACTGCGTCTCCCAACCCTCTCATCGGATTGGtttt                                   <  2:864843/243‑1 (MQ=255)                                                                                                                                                                                                                           gccTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGAGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCCGCGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACCATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGtttt                                   >  2:360242/1‑251 (MQ=255)                                                                                                                                                                                                                             cTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTAGTTTTc                                  >  2:13260/1‑250 (MQ=255)                                                                                                                                                                                                                               tCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAg                                <  1:291204/250‑1 (MQ=255)                                                                                                                                                                                                                                cTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCACCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTCTTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGCATTGGTTTTCAGCt                              >  2:727334/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACC‑GAAACTTCGGCTCCCATCCCTTTCATCGGATTGGTTTTCAGCtt                             >  2:227148/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTg                            <  1:770519/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAAAGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGAGGCTACCATCGCTTTCAACGGATTGGttttacacttgc                           >  2:681726/1‑243 (MQ=255)                                                                                                                                                                                                                                   cGTCGCTACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGGAGCTCTTCATGGGTGCACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GGAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTAAGCttcc                           >  2:478931/1‑249 (MQ=255)                                                                                                                                                                                                                                         tACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCCTCGGATTGGTTTTCAGCTTGCCGtttt                     >  2:502142/1‑251 (MQ=255)                                                                                                                                                                                                                                         tACAATGGATTCGAT‑T‑CC‑C‑CCT‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGtttt                     >  2:369223/1‑251 (MQ=255)                                                                                                                                                                                                                                           cattGGATTCGAT‑T‑CC‑C‑CCG‑CGGGCCAGCGCCAACAAGAGGAGTAGCTCTTCATGGGTTAGCGGCTCGCCGTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACGACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCTCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTGCAGCTTGCCGTTTTcc                   <  2:459738/248‑1 (MQ=255)                                                                                                                                                                                                                                            aaTGGATTCGTT‑G‑CC‑C‑CCT‑CGGGCCAAAGCCAACAAGATGAGTAGCTTTTCATGGGTTAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTTTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCTGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCAGCGGATTGGTTTTCAGCTTGCCGTTTTcca                  <  2:721154/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cgat‑t‑cC‑C‑CCT‑CGGACCAGAGCCAACAAGATGAGTAGCTCTTCATGGGGGCACGGCTCGCCTTCTGCCGTACCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCCACGTATTCCAGATCGCACACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCCTCGGATTGTTCTTAAGCTTGACGTTTTCCACCACCTTc          >  2:665263/1‑251 (MQ=255)                                                                                                                                                                                                                                                       attccc‑c‑c‑T‑CGGGCCAGAGCCAACAAGATGATTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTCCCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTg        <  2:262834/247‑1 (MQ=255)                                                                                                                                                                                                                                                          ttcccac‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGGCATCACTACGTTAATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCGAACTCGCAATCGGCTTCGCCGTTCACAATTCCGACA‑GAAACTACGGCAACCATCCCTTTCATAGGCATGGTATTCAGCTTGCCTTTTTACACCAGCTTcgt        >  2:802448/7‑248 (MQ=255)                                                                                                                                                                                                                                                          ccc‑c‑c‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCACCTTCTACTGTCCCCTGCACTTCAATGATACACCCGTCTTCGGTCATCACTACGCTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCACTTCGCCGTTCAAAATTCCACCA‑GACACTGCGGCTCCCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGt       >  1:52036/2‑249 (MQ=255)                                                                                                                                                                                                                                                              c‑c‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAgcgc  >  1:32581/1‑251 (MQ=255)                                                                                                                                                                                                                                                                c‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAgcgc  >  1:349340/1‑250 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                            ATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGC‑TTTTGCGCCCGGATTTCA‑GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT‑T‑CC‑C‑C‑T‑CGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACA‑GAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGC  >  NC_000913/3815634‑3816132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑