Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913480,2950GA100.0% 112.6 / NA 34P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/13);  total (21/13)
*NC_000913480,2960GA100.0% 114.6 / NA 34P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/13);  total (21/13)

GGTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTT  >  NC_000913/480062‑480521
                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                  
ggTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGGT‑CATTATCTGATAATTCGTATTTATTTTTCTTGTTAACACACTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATaatt                                                                                                                                                                                                                    >  1:2097/1‑248 (MQ=255)
      aGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCt                                                                                                                                                                                                             >  1:400378/1‑251 (MQ=255)
         cccGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATTTTGTCGTACAGTTTATTCAGGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATg                                                                                                                                                                                                          <  1:363492/250‑1 (MQ=255)
            taaaGCGAATTGTTTATTAATGCATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTTGGCGAGGCGGTCGTCTGCGGGAAGGGAAAATACCGCCAGTTTCTTTGT‑TGATAATTCGAGTTTATTTTTCTCGATAACACGTTCCCGCGTGTTAATTTTCTGGATACGACTGTAAA‑TCATTAAATAATAGGGTTTGGTTAAAAATTTTTTGGAC‑TATCTTCTAccactggtt                                                                                                                                                                                                       >  2:373717/2‑244 (MQ=255)
              aaGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGt                                                                                                                                                                                                     <  1:39905/251‑1 (MQ=255)
                                           ccATACTGAGGAGGGGATCTTGTCTGACATTTGATTAATGGCCAATTCGGCGGGGCGGTGATCTGCGGCTGAGTAAAATCCCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGa                                                                                                                                                                        <  2:158644/251‑1 (MQ=255)
                                               aCTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATtct                                                                                                                                                                    >  1:164526/1‑251 (MQ=255)
                                                          gATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTTCGGCTGAGTAAAATACCGCCAGGT‑CATTATCTGATAATTCGGATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGAAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTGCGGAAATA‑ATTATACCTATGGTTGTAATAATTCTAACAAAATAAAATAGCAGGATTCTCTTTTGTCGcc                                                                                                                                                         >  1:688052/1‑250 (MQ=255)
                                                          gATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGGAAAATACCGCCAGTT‑CATTATATGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGGGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTAGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGAAGGATTTTCTTtcgtcccc                                                                                                                                                         >  1:258939/1‑248 (MQ=255)
                                                                gtcggataGTTTATTCATGGTCAATCCGGCGAGTCGTTGATCTGCGTCTGAGAAAAATACCGCCAGTT‑CATTATCTGATAAGTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGTACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGAGTCTCTTTCGTCGCATTGACa                                                                                                                                                   <  2:341646/244‑1 (MQ=255)
                                                                            aTTCATGTTCAATTCGGCGAGGCGGTAATCTGCGCCTGAGTAAAATACCGCCAGAT‑CATTATCTGATAATTCGTATTTTTTTTTCTAGATAAAACGCGCCCACGGGTCAATTTTCTGGCAACGGC‑GTCACAGCATTAACTAATAGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAATCCAAGGATTCTCTTtcttgggcttgacaaacacacggaa                                                                                                                                        >  2:454118/1‑227 (MQ=255)
                                                                                              gAGGCGGTGGTCTGCGGCTGAGTAACATACCGCCAGTT‑CATTATCTGAAAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GGAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGAATCTCTTTCGTCGCATTTACAAAACAACGGAATAGTCGATTACCTGACttt                                                                                                                     >  2:585042/1‑250 (MQ=255)
                                                                                               gggcgtggttttgggtcTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTcc                                                                                                                    <  2:217500/235‑1 (MQ=255)
                                                                                                   ggTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGccc                                                                                                                >  1:238290/1‑250 (MQ=255)
                                                                                                                tGAGTAAAATACCGACAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCACCGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTACTAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCTTTGACAACACAACGGAATAGTCGATTACCTAACTTCCACAATTTCTGAAGCTcc                                                                                                   >  2:522555/1‑251 (MQ=255)
                                                                                                                                aGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                                                                                   >  1:273177/1‑251 (MQ=255)
                                                                                                                                          tCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATACTTCTACCAAGATAAACTCGCAGGCTTCTCTTTCGTCGCATTGAAAAACCAACGGAATAGTCGATTACCTGAATTCCGCCATTTCTGAAGATCCTGCATATTAATCCCATAactattg                                                                           >  2:671463/1‑247 (MQ=255)
                                                                                                                                             gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                                                                         <  1:693136/249‑1 (MQ=255)
                                                                                                                                             gATAATTCGTATTTAATTTTTTTGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCTGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGGCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTCCTGaac                                                                        <  2:507400/250‑1 (MQ=255)
                                                                                                                                                   tCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCAttt                                                                                                             >  1:49614/1‑207 (MQ=255)
                                                                                                                                                   tCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCAttt                                                                                                             <  2:49614/207‑1 (MQ=255)
                                                                                                                                                     gTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaa                                                                 >  1:654274/1‑249 (MQ=255)
                                                                                                                                                              tttCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAACTAATCGGTTTTCGTTAAAAATTTTTCGGACATC‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTCCCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtgacttcc                                                      >  2:321975/1‑245 (MQ=255)
                                                                                                                                                                tctcGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCa                                                     <  2:164526/250‑1 (MQ=255)
                                                                                                                                                                             cTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCATCGATc                                       >  1:360915/1‑251 (MQ=255)
                                                                                                                                                                               ccAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCAAGATATTCGTCGATCTg                                     >  1:286438/1‑251 (MQ=255)
                                                                                                                                                                                         aaTTGTCTGGCAACGAC‑GTAAACGCATTAAAAAATAGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGc                           >  2:645165/1‑251 (MQ=255)
                                                                                                                                                                                                 ggCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGcc                                                                                                                 >  2:309770/1‑157 (MQ=255)
                                                                                                                                                                                                 ggCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTAtt                      <  1:294083/248‑1 (MQ=255)
                                                                                                                                                                                                 ggCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCATGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTc                   >  1:157150/1‑251 (MQ=255)
                                                                                                                                                                                                               actcATTAAAAAAGCGGTTTTCGTTAAAAACTTTGCGGACATA‑CTTCTACCTATGGTTGTAAAAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGACGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTACGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCGTATTGGCTGCATTATACTTa      <  2:525273/248‑1 (MQ=255)
                                                                                                                                                                                                                  cATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAg                                                                                                                                     <  2:386574/121‑1 (MQ=255)
                                                                                                                                                                                                                  cATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAg                                                                                                                                     >  1:386574/1‑121 (MQ=255)
                                                                                                                                                                                                                   agtAAATAATCTGTTTTCGTTAAAAATGTGTCTGACATA‑CTTCTACCTATGGTTGGAATAATTCTAACAAGATAAAAGCGCAGGATTCTCTTCCGTCGCATTGAAAAAACAACTGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATTCCATAACTACTGAACAGCATAAAGGGGTCATCCAGATATTCGTCGAACTGCTCAATGAGCTTATTGTCTTCCTTCTACTTaagtg  <  2:129750/249‑4 (MQ=255)
                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                  
GGTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTT‑CATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGAC‑GTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATA‑CTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTT  >  NC_000913/480062‑480521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑