Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,400	G→A	L217L (CTG→TTG)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,400	0	G	A	96.5%	175.0 / ‑4.1	58	L217L (CTG→TTG)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (29/26);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCC  >  NC_000913/69155‑69634                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            gCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCATACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAgccgc                                                                                                                                                                                                                                        >  1:318015/1‑251 (MQ=255)   aGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGccc                                                                                                                                                                                                                                      >  1:486348/1‑251 (MQ=255)      tGGAAGTACCGCTAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGCGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGGGAACAGCGGGGAAGGAAAATGGCGGTTGAGGATCGGGTCCCGCTCAACCAAGAAACTGGCTGGCGGCAATCCGcccccg                                                                                                                                                                                                                                   >  2:11674/1‑249 (MQ=255)        gAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCt                                                                                                                                                                                                                                 >  1:505012/1‑251 (MQ=255)            tACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGACACTGGCTGGCGGCAAGCCGCCCCAGCTTTCg                                                                                                                                                                                                                             >  1:297706/1‑251 (MQ=255)             aCCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGt                                                                                                                                                                                                                            <  1:312824/251‑1 (MQ=255)                     cTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCCGCTTTCGTGCcac                                                                                                                                                                                                                       >  1:448264/1‑248 (MQ=255)                       tttACCAGGGCGTTAGGCTGGGCCCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCTCCGCCGGAAATCCCCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACag‑a                                                                                                                                                                                                                   <  2:226948/249‑1 (MQ=255)                             agcgCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAAATGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGAtttttg                                                                                                                                                                                                           >  2:671868/4‑248 (MQ=255)                              gTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCACACGCGCCGACGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGTCAGTCCAGGTGTCAGTGAACAGCGGGGTAGACAAATCGCGATTTAGGTTCTGTTCCAACTCATCATAGAAACTGTCTGGCTGCAACCCGCCCCAGCtttttgtccacagctttttttac                                                                                                                                                                                                           >  2:368975/1‑231 (MQ=255)                               tGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGcc                                                                                                                                                                                                         >  1:471775/1‑251 (MQ=255)                                   gtAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGgc                                                                                                                                                                                                     <  1:368975/250‑1 (MQ=255)                                         tgtgCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAACTCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAAGTGCCCACCGGAATATAGGCAGGCCAGGAGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCCCATCAAAGAAACAGGCGTGCGGCGCGCCACCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCCgcgcgac                                                                                                                                                                                                >  2:56896/1‑247 (MQ=255)                                           tgcgcctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGGGCCCCCG‑GGATTTATGCCCGGCGCTGCAAc                                                                                                                                                                                             >  2:117560/1‑251 (MQ=255)                                               cctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTcc                                                                                                                                                                                         <  1:163447/251‑1 (MQ=255)                                                ctgcgccAGCTGCGCCCATATGGCAGTCAAACTCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAAAGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAAATGGCTGGGGGCAAGCCGCCCCAGCTTTCGTGCCACCG‑GGATTTATGCCCGGAGCAGCAACGTccc                                                                                                                                                                                        >  2:587025/1‑250 (MQ=255)                                                  gcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTGTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCcgcg                                                                                                                                                                                      <  1:655863/251‑1 (MQ=255)                                                    gccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTAt                                                                                                                                                                                                            >  1:349250/1‑227 (MQ=255)                                                    gccAACTGCGCCCATATGGCAGCCAAACGCGCCGCCGGAAATCCCCACGCTGTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGGGGCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTAt                                                                                                                                                                                                            <  2:349250/227‑1 (MQ=255)                                                      cAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGcgacga                                                                                                                                                                                  <  1:294310/251‑1 (MQ=255)                                                                            caaCGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCCGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCCTCAAAGAAACTGGCTGGCGGCCAGCCGCCCCAGCCTTCGTGCCCCAG‑AGATTTATGCCCGGCCCTGCAACGTCCGCGGCGAATATCCTgcgggcggcggggcc                                                                                                                                                            >  2:64586/2‑244 (MQ=255)                                                                                     gccgGAACTCACCACGCTTTCAGGCAGACCGAGACGCTGCGCCCATTCCGGGAATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCa                                                                                                                                                   <  2:486348/251‑1 (MQ=255)                                                                                             tccccacGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATg                                                                                                                                                                                                           <  2:464218/184‑1 (MQ=255)                                                                                             tCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATg                                                                                                                                                                                                           >  1:464218/1‑187 (MQ=255)                                                                                                 cactcTTTCAGGGAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCTCCGTAATATCGGCGGTCCAGGTGTCAGTGAACAGCGGGTAAGGCAAATGGCGATGGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTGTCGTGCCACAG‑AGATTTATGCCCGGCGCGGCACCGTCCGTGACGAATATCCTGCGGTCGGGTGGTACCGCAAAGCAGTGCTGGCAccc                                                                                                                                       <  2:635783/247‑1 (MQ=255)                                                                                                 cacGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCAccc                                                                                                                                       <  1:448302/251‑1 (MQ=255)                                                                                                           ggcaggcCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAg                                                                                                                             <  1:117560/251‑1 (MQ=255)                                                                                                                gcCGAGACGCTGCGCCCATTACGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGAAAGCCGCCCCAGCTTTCGTGCCACAG‑GGAATTATGCCCGGCGTTGCAACGGCCGAGACGAATATCCTGCGGTGGGGTGGCAACGGAAAGCaaacaaaccgaagatcgtcgggtag                                                                                                                           >  2:424118/1‑224 (MQ=255)                                                                                                                gacGAGACGCTGCGCCCATTCCGGGCATAAGGGGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCTGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCACTGCGCTGCAACGTCCGTGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCaga                                                                                                                                                 <  1:424118/224‑1 (MQ=255)                                                                                                                   gagaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGACCCAGCTTTCGTGCCCAAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCGGGCACCCAGTCGCACAGGCCAATcc                                                                                                                     >  2:422765/1‑251 (MQ=255)                                                                                                                    agaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATcc                                                                                                                     >  1:115981/1‑250 (MQ=255)                                                                                                                     gaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCAc                                                                                                                   <  1:127889/251‑1 (MQ=255)                                                                                                                      aCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACg                                                                                                                  >  1:254461/1‑251 (MQ=255)                                                                                                                             gcCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGAAGTCCAGGGGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGCTCGGGTCCAGATCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACCG‑AGATTTATGCCCGGCGCAGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCAGCCAGTCGCACAGCTCCAACCACGATGCGGc                                                                                                           >  1:368174/1‑251 (MQ=255)                                                                                                                               ccATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGc                                                                                                           >  1:486132/1‑249 (MQ=255)                                                                                                                                catgccGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGa                                                                                                        <  2:701974/247‑1 (MQ=255)                                                                                                                                     cGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTgcg                                                                                                   >  1:29227/1‑251 (MQ=255)                                                                                                                                       ggCATAAGGTGCCCCCCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGcc                                                                                                 <  2:297706/251‑1 (MQ=255)                                                                                                                                         cATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGAGCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCAc                                                                                               <  1:85274/251‑1 (MQ=255)                                                                                                                                                 gCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTg                                                                                        <  1:568311/250‑1 (MQ=255)                                                                                                                                                   ccACCGGAACATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTcc                                                                                     <  1:49036/251‑1 (MQ=255)                                                                                                                                                           atatCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGt                                                                             <  1:431156/251‑1 (MQ=255)                                                                                                                                                             atCGGCAGTCCAGGTGTCAGTGAACAGAGGGGAAGGCAAATGGCGATGGAGGATCGGGGCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGGCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTca                                                                           <  2:109807/251‑1 (MQ=255)                                                                                                                                                             atCGGCAGTCCAGGGTTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTc                                                                            <  1:649295/250‑1 (MQ=255)                                                                                                                                                                    gtccaggggccAGTGAACAGCGGGGAAAGCAAAGGGCGATTGAGGATCGTGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCATCTTTCGTGCCACAG‑AGAGTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGGGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTTGCGAGTCACATGCAg                                                                    <  2:491471/241‑1 (MQ=255)                                                                                                                                                                            gtCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGAttttt                                                             <  1:407710/250‑1 (MQ=255)                                                                                                                                                                                           ggAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGGGTCACATGCAGGCGTTTTGCCCAGAACCATTCGc                                             >  1:498844/1‑251 (MQ=255)                                                                                                                                                                                            gAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCCGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCCCGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTTCCCAGCACCATTCGCt                                            >  2:541647/1‑251 (MQ=255)                                                                                                                                                                                                 cAAATGGCGATTGAGGTGCGGTGCCAGTGCCTCAAAGAAACTGGCTGACGGTAGGCAGCCCCAGCTTGCGTGGCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATAGCCTGTGGGCGGGTGGTACCGGAAAGCAGAGCTGGCTCCCAGTCGCACAGCGCAATCCACGATGCGTCAGATTGCGCCACGGCGATGTGCTGGCGAGGCACATGCAGTATTTTTGCCCAGAACCACTCGCTGGaat                                       <  2:85687/251‑1 (MQ=255)                                                                                                                                                                                                   aaTGGCGATTGAGGATCGGGTCCATCTCCTCAAAGAAACTGGCTGGCGGCAAGCCGCCCCCGCTTTCGTGCCACAG‑AGATTTATTCCCGGCGCTGCAACGTCCGCGACGAATATCCTCCGGGCGGGTGGGACCGGAAAGGCGAGCTTGCACCCAGTCGCCCAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGACATCCTGGCGAGTCACCGTCAGGGATTTTGCCCCGAACCACTCGCTTGaata                                      >  2:106201/1‑250 (MQ=255)                                                                                                                                                                                                     tGGCGATTGAGGAGCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGGACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGCATGCGCCCCGGAGCCGGCCTGGCGGGACACATGCAAGATTTTTGCCCAGAACCCTTCGCTGGaaaaaa                                    >  2:706730/1‑250 (MQ=255)                                                                                                                                                                                                       gCGATTGAGGATCGGGTCCAGCTCATTAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATac                                 <  2:350240/251‑1 (MQ=255)                                                                                                                                                                                                         gATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAATTaccg                               >  1:196321/1‑250 (MQ=255)                                                                                                                                                                                                              aGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATaccgcccat                          >  1:134483/1‑245 (MQ=255)                                                                                                                                                                                                                                    aGAAACTGGCGGGCGGCAAGCCCCCCCAGCTTTCG‑GTCCCAGTAGATTGTGGCGCGGCCCGGCAACGTCCGCGACGAATATCCGGGGGGCGGGTGGTACCGGAAAGCAGGGCTGGCCCCCATCCGCACAGCCCAATCCACGAGGCGGCATATTGCGCCAGGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGTTGGAAAAAAAACCGCCAATATAGCGGGAGTAGTCAACATTg    <  2:590467/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaaCTGGCTAGCGGCAAGCCGCCCCAGCTTTCGTGCCTCAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCCCGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATATATCCCGCCAATCTAGCGGGAGGAGTTAACATTGcc  >  2:304659/1‑251 (MQ=255)                                                                                                                                                                                                                                             ctggcGGCAAGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACgg                                                                                             >  1:156695/1‑153 (MQ=255)                                                                                                                                                                                                                                             ctggcGGCAAGCCGCCCCAGCGTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGTCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACgg                                                                                             <  2:156695/153‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            GCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAG‑AGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCC  >  NC_000913/69155‑69634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑