Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,323	A→G	T242T (ACT→ACC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,323	0	A	G	98.6%	229.8 / ‑4.4	72	T242T (ACT→ACC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (32/39);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

CCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCA  >  NC_000913/69076‑69561                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ccacGCTGCCATCAACCTGACCGCAAATTCCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGgtg                                                                                                                                                                                                                                             <  1:490917/251‑1 (MQ=255)      cTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGACGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGGCCCGGTGTCAgg                                                                                                                                                                                                                                        >  2:29811/1‑250 (MQ=255)       tGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTg                                                                                                                                                                                                                                       >  1:350240/1‑251 (MQ=255)        gCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGGCCAGGGGTCAGTg                                                                                                                                                                                                                                       >  2:125049/1‑250 (MQ=255)          cATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGATAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCCGGTGTCAGTGAAc                                                                                                                                                                                                                                    >  1:598567/1‑251 (MQ=255)                  tGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCAGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCgggg                                                                                                                                                                                                                             >  1:299012/1‑250 (MQ=255)                          aaTACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGGCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAAt                                                                                                                                                                                                                    <  1:100993/251‑1 (MQ=255)                             ccatttAACTGCCCGCTCGCAAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCGGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCGGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTAAGTGAACAGCGGGGAAGGCAAATGGc                                                                                                                                                                                                                 <  2:34418/248‑1 (MQ=255)                              ccTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCg                                                                                                                                                                                                                <  1:125049/251‑1 (MQ=255)                                  tAACGGCCCGCTCGCCAACGCTCTGGTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCGGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGAtt                                                                                                                                                                                                             <  2:322224/250‑1 (MQ=255)                                   aaCTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGGCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTg                                                                                                                                                                                                            <  2:305656/250‑1 (MQ=255)                                       gCCCGCTCGCCAACGCTCTGTTTGTCGGTAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGAt                                                                                                                                                                                                       <  1:277837/251‑1 (MQ=255)                                          cGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCgg                                                                                                                                                                                                    <  1:29811/251‑1 (MQ=255)                                             tCGCCAACGCTCTGTTTGTCGGCAATCAGAATGACGCAGGTGGAAGTACCGATAACTTTTAACAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGCAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCCGGTGGCCGTGAACAGCGGGGAAGGCAAAGTGCGATTGAGGATCGGGTc                                                                                                                                                                                                 >  2:407710/1‑251 (MQ=255)                                                     gcgctGTTTGTCGGCAATCCGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCTTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTGTCAGGCAGGCCGAGACGCTGCGCCCAGTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCtcat                                                                                                                                                                                         <  2:630822/248‑1 (MQ=255)                                                          gTTTGTCGGCATTCAGAATGTTGCAGGTGGAAGTACCGATAACGTTTACAAGTGCGTTAGTCTGGTCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTTAGGTTCGGGGCTAGCtcatca                                                                                                                                                                                       <  2:312909/248‑1 (MQ=255)                                                          gTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCaaa                                                                                                                                                                                     <  1:339805/250‑1 (MQ=255)                                                            ttgccggcacaccgAATGTCGCAGGTGGAAGTACCTATAACTTTTACCAGTGCGTTAGGCTGCGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGATATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGa                                                                                                                                                                                   <  2:178036/237‑1 (MQ=255)                                                            ttGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGa                                                                                                                                                                                   <  1:257437/250‑1 (MQ=255)                                                                cGGCAATCAGAATGTCGCAGGTGGAAGTACCTATAACTTTTTCCAGTTCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACTCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACGGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAActg                                                                                                                                                                              <  1:347869/251‑1 (MQ=255)                                                                        aGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATAACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAATGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAACTGGCGGTTGAGGCACGGGTCCAGCCCAACaaaacaacacgctggcgg                                                                                                                                                                      >  2:448302/1‑236 (MQ=255)                                                                           aTGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCa                                                                                                                                                                    >  1:590467/1‑250 (MQ=255)                                                                                gCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCATACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAgccgc                                                                                                                                                              >  1:318015/1‑251 (MQ=255)                                                                                  aGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGccc                                                                                                                                                            >  1:486348/1‑251 (MQ=255)                                                                                     tGGAAGTACCGCTAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGCGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGGGAACAGCGGGGAAGGAAAATGGCGGTTGAGGATCGGGTCCCGCTCAACCAAGAAACTGGCTGGCGGCAATCCGcccccg                                                                                                                                                         >  2:11674/1‑249 (MQ=255)                                                                                       gAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCt                                                                                                                                                       >  1:505012/1‑251 (MQ=255)                                                                                           tACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGACACTGGCTGGCGGCAAGCCGCCCCAGCTTTCg                                                                                                                                                   >  1:297706/1‑251 (MQ=255)                                                                                            aCCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGt                                                                                                                                                  <  1:312824/251‑1 (MQ=255)                                                                                              cGATAACTTTTACCAGTGCGTTAGGCTGAGCGCCTGCGCCAACTGCGCCCATATGGAAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAGGGTGCCCACCGGAATATAGGCAGTCCAGGGGCCAGTGAACAGCGGGGGAGGCAAATGGCGATTTGGGCTCGGGTCCAACTCATGCAAGAAACTGGTGggcggcgaggcgcacaggcaatcaggc                                                                                                                                                >  2:629945/1‑230 (MQ=255)                                                                                                    cTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCCGCTTTCGTGCcac                                                                                                                                             >  1:448264/1‑248 (MQ=255)                                                                                                      tttACCAGGGCGTTAGGCTGGGCCCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCTCCGCCGGAAATCCCCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACaga                                                                                                                                          <  2:226948/249‑1 (MQ=255)                                                                                                            agcgCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAAATGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGAtttttg                                                                                                                                  >  2:671868/4‑248 (MQ=255)                                                                                                            aGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAActggc                                                                                                                                                                            <  1:142488/209‑1 (MQ=255)                                                                                                             gTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCACACGCGCCGACGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGTCAGTCCAGGTGTCAGTGAACAGCGGGGTAGACAAATCGCGATTTAGGTTCTGTTCCAACTCATCATAGAAACTGTCTGGCTGCAACCCGCCCCAGCtttttgtccacagctttttttac                                                                                                                                 >  2:368975/1‑231 (MQ=255)                                                                                                              tGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGcc                                                                                                                                >  1:471775/1‑251 (MQ=255)                                                                                                                  gtAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGgc                                                                                                                            <  1:368975/250‑1 (MQ=255)                                                                                                                        tgtgCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAACTCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAAGTGCCCACCGGAATATAGGCAGGCCAGGAGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCCCATCAAAGAAACAGGCGTGCGGCGCGCCACCCCAGCTTTCGTGCCACAGAGATTTATGCCCCgcgcgac                                                                                                                       >  2:56896/1‑247 (MQ=255)                                                                                                                          tgcgcctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGGGCCCCCGGGATTTATGCCCGGCGCTGCAAc                                                                                                                    >  2:117560/1‑251 (MQ=255)                                                                                                                              cctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTcc                                                                                                                <  1:163447/251‑1 (MQ=255)                                                                                                                               ctgcgccAGCTGCGCCCATATGGCAGTCAAACTCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAAAGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAAATGGCTGGGGGCAAGCCGCCCCAGCTTTCGTGCCACCGGGATTTATGCCCGGAGCAGCAACGTccc                                                                                                               >  2:587025/1‑250 (MQ=255)                                                                                                                                 gcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTGTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCcgcg                                                                                                             <  1:655863/251‑1 (MQ=255)                                                                                                                                   gccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTAt                                                                                                                                   >  1:349250/1‑227 (MQ=255)                                                                                                                                   gccAACTGCGCCCATATGGCAGCCAAACGCGCCGCCGGAAATCCCCACGCTGTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGGGGCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTAt                                                                                                                                   <  2:349250/227‑1 (MQ=255)                                                                                                                                     cAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGcgacga                                                                                                         <  1:294310/251‑1 (MQ=255)                                                                                                                                                           caaCGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCCGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCCTCAAAGAAACTGGCTGGCGGCCAGCCGCCCCAGCCTTCGTGCCCCAGAGATTTATGCCCGGCCCTGCAACGTCCGCGGCGAATATCCTgcgggcggcggggcc                                                                                   >  2:64586/2‑244 (MQ=255)                                                                                                                                                           aaaCGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATCAGGTGCACACCGGAATATCGGCAGTCCAGGTGTCAGTGACCAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCACAGa                                                                                                                                                                                   >  2:423897/1‑155 (MQ=255)                                                                                                                                                           aaaCGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGa                                                                                                                                                                                   <  1:423897/155‑1 (MQ=255)                                                                                                                                                                    gccgGAACTCACCACGCTTTCAGGCAGACCGAGACGCTGCGCCCATTCCGGGAATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCa                                                                          <  2:486348/251‑1 (MQ=255)                                                                                                                                                                            tccccacGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATg                                                                                                                                  <  2:464218/184‑1 (MQ=255)                                                                                                                                                                            tCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATg                                                                                                                                  >  1:464218/1‑187 (MQ=255)                                                                                                                                                                                cactcTTTCAGGGAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCTCCGTAATATCGGCGGTCCAGGTGTCAGTGAACAGCGGGTAAGGCAAATGGCGATGGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTGTCGTGCCACAGAGATTTATGCCCGGCGCGGCACCGTCCGTGACGAATATCCTGCGGTCGGGTGGTACCGCAAAGCAGTGCTGGCAccc                                                              <  2:635783/247‑1 (MQ=255)                                                                                                                                                                                cacGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCAccc                                                              <  1:448302/251‑1 (MQ=255)                                                                                                                                                                                          ggcaggcCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAg                                                    <  1:117560/251‑1 (MQ=255)                                                                                                                                                                                               gcCGAGACGCTGCGCCCATTACGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGAAAGCCGCCCCAGCTTTCGTGCCACAGGGAATTATGCCCGGCGTTGCAACGGCCGAGACGAATATCCTGCGGTGGGGTGGCAACGGAAAGCaaacaaaccgaagatcgtcgggtag                                                  >  2:424118/1‑224 (MQ=255)                                                                                                                                                                                               gacGAGACGCTGCGCCCATTCCGGGCATAAGGGGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCTGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCACTGCGCTGCAACGTCCGTGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCaga                                                                        <  1:424118/224‑1 (MQ=255)                                                                                                                                                                                                  gagaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGACCCAGCTTTCGTGCCCAAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCGGGCACCCAGTCGCACAGGCCAATcc                                            >  2:422765/1‑251 (MQ=255)                                                                                                                                                                                                   agaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATcc                                            >  1:115981/1‑250 (MQ=255)                                                                                                                                                                                                    gaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCAc                                          <  1:127889/251‑1 (MQ=255)                                                                                                                                                                                                     aCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACg                                         >  1:254461/1‑251 (MQ=255)                                                                                                                                                                                                            gcCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCaag                                                                                                                                                                  >  2:275395/1‑121 (MQ=255)                                                                                                                                                                                                            gcCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCaag                                                                                                                                                                  <  1:275395/123‑3 (MQ=255)                                                                                                                                                                                                            gcCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGAAGTCCAGGGGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGCTCGGGTCCAGATCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACCGAGATTTATGCCCGGCGCAGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCAGCCAGTCGCACAGCTCCAACCACGATGCGGc                                  >  1:368174/1‑251 (MQ=255)                                                                                                                                                                                                              ccATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGc                                  >  1:486132/1‑249 (MQ=255)                                                                                                                                                                                                               catgccGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGa                               <  2:701974/247‑1 (MQ=255)                                                                                                                                                                                                                    cGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTgcg                          >  1:29227/1‑251 (MQ=255)                                                                                                                                                                                                                      ggCATAAGGTGCCCCCCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGcc                        <  2:297706/251‑1 (MQ=255)                                                                                                                                                                                                                        cATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGAGCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCAc                      <  1:85274/251‑1 (MQ=255)                                                                                                                                                                                                                                gCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTg               <  1:568311/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccACCGGAACATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTcc            <  1:49036/251‑1 (MQ=255)                                                                                                                                                                                                                                          atatCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGt    <  1:431156/251‑1 (MQ=255)                                                                                                                                                                                                                                            atCGGCAGTCCAGGTGTCAGTGAACAGAGGGGAAGGCAAATGGCGATGGAGGATCGGGGCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGGCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTca  <  2:109807/251‑1 (MQ=255)                                                                                                                                                                                                                                            atCGGCAGTCCAGGGTTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTc   <  1:649295/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               CCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCA  >  NC_000913/69076‑69561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑