BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,849	G→A	G400G (GGC→GGT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,849	0	G	A	96.2%	237.4 / ‑2.9	79	G400G (GGC→GGT)	araB	ribulokinase
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (30/45); total (33/46)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACC‑AAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCAC  >  NC_000913/68614‑69076

ggCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCg                                                                                                                                                                                                                           <  1:504639/251‑1 (MQ=255)

ggCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGGTTGGCGAGCGACGACCGTTAAACCAGTCg                                                                                                                                                                                                                           <  1:189573/251‑1 (MQ=255)

ggCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGGTTGTCCGCGCGATGCCGCCCAGCGCCAGCACGTTATTGACGGCGAGCCCCTGATCGGTAAAGCACTCCAGGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTGTCAGGCGTTGGGTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCg                                                                                                                                                                                                                           <  2:92118/251‑1 (MQ=255)

 gCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGa                                                                                                                                                                                                                          <  1:262238/251‑1 (MQ=255)

    aTTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGGAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAgg                                                                                                                                                                                                                        >  1:527419/1‑249 (MQ=255)

        aGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGcaccac                                                                                                                                                                                                                   <  1:74753/251‑1 (MQ=255)

        aGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGcaccac                                                                                                                                                                                                                   <  1:68970/251‑1 (MQ=255)

                  agcagGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCACCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGa                                                                                                                                                                                                       <  1:687167/251‑1 (MQ=255)

                     gggCCTACATAATGCCCTGTTTTTGCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGCCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGGAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATcc                                                                                                                                                                                                    <  2:21821/250‑1 (MQ=255)

                     agGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCACAGGCGGTGGCAGCAATCCAACC‑GCCGACCAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTCATCACCCCTTTCCGGCGTTGGTTAGCGTTCGGAGTGCGGCGGCCGTTAAACCAGTCGAGCCCCACCGGC‑Aggggtgac                                                                                                                                                                                                     >  2:345656/1‑245 (MQ=255)

                     agGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATcc                                                                                                                                                                                                    >  1:484022/1‑251 (MQ=255)

                      ggCCTGCATAATGTCCTGGTTTTGCCGCGGGATGCCGCCCAGCGCCATCACGTGTTGGACGGCGATCCCCTGATCGGTAAAGCACTCCAGGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCa                                                                                                                                                                                                   <  2:157040/251‑1 (MQ=255)

                        ccTGCATAATGACCTGGTTTTTCCGCGCGAGGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCGGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCaga                                                                                                                                                                                                 <  2:385278/251‑1 (MQ=255)

                           tcaaaaTGACCTGGTTTTTCCGCCCGCTGCCTGCCAGCTCCATCACGTTATTGACGGCGACCCCCGGAACGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGAGGCATAAATCATACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCagag                                                                                                                                                                                                <  2:263589/245‑1 (MQ=255)

                           gCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCagag                                                                                                                                                                                                <  1:399587/249‑1 (MQ=255)

                               aaTGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑Ga                                                                                                                                                                                         <  1:164948/250‑1 (MQ=255)

                                          ttttCCGCGCGATTCCGCCCAGCGCCATCACTTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGTGCGTGCGTCAAAGGCGGTGGAAGCAATCAAACC‑GCCGAACAGCAGCTGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCCCCCCTTTCAGGCGTTGGTTAGCGTTTTGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CT‑GATTTTTTGCCCa                                                                                                                                                                              <  2:481816/250‑1 (MQ=255)

                                                 cgcgATGCCACCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGCTCGGTAATCACCCCTTTCAGGCGGTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCCGTCGAGCACCCCCGGC‑AGG‑TGATCCCGAAA‑CG‑GGTTTTTTGCCCCTGCTTCgg                                                                                                                                                                      >  2:140861/1‑251 (MQ=255)

                                                    gATGCCGCCCAGCGCCAGCACGTTATTGACGGCGACCCCCTGATGGGTAAAGCCCTCCATGATTGCGCGTGCGCCAAAGGGGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCa                                                                                                                                                                   <  2:672452/251‑1 (MQ=255)

                                                        ccgccCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAgcgc                                                                                                                                                               >  1:500518/1‑251 (MQ=255)

                                                              agtcccaccctgttattgccGGCGATCCCCTGGTCGGTAAAGCAATCCCTGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAAACAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGGTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTGAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAg                                                                                                                                                         <  2:500557/232‑1 (MQ=255)

                                                                cgCCATCACGTGATTGACGGCGATCCCCGGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCTGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCa                                                                                                                                                       <  1:468951/250‑1 (MQ=255)

                                                                 gCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGCGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGGAGccg                                                                                                                                                      >  2:702619/1‑249 (MQ=255)

                                                                   cagcACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAgtt                                                                                                                                                    <  1:331448/248‑1 (MQ=255)

                                                                   cATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GCTTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAggt                                                                                                                                                    >  1:589014/1‑249 (MQ=255)

                                                                   cATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAgtt                                                                                                                                                    <  1:571402/251‑1 (MQ=255)

                                                                   cATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAACGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTTGCGAGCGGCGACCGTTTACCCAGTCGAGCACCACCGGC‑AGGGTG‑TCCAG‑GAGCG‑GATTTTTGGCCCCAGCTTCGGTCCACGCCGGAAGCAggg                                                                                                                                                    >  2:459904/1‑249 (MQ=255)

                                                                    aTCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCCATCAAACC‑GCCAGACAGCAGCGGAGCGTCGGTAGCGGGGTTAAGCTCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGAGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑GGGTCCCGGGA‑CG‑GGTTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAgttg                                                                                                                                                   >  2:302436/1‑251 (MQ=255)

                                                                             tggagggcgccccccTGATGGGGAAAGCTCTCCATGCTTGGGGGTGCGCAAAAGTCGTGGGCAGCAATCAACCC‑GCCGAACAGCAGCGGAGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGGTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTGGTGTCCAATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTctggc                                                                                                                                           <  2:414361/239‑1 (MQ=255)

                                                                               gaCGGCGACCCCCTGATCGGTAAAGCACTCCAGGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTctggctg                                                                                                                                         <  2:110529/249‑1 (MQ=255)

                                                                                    cGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTg                                                                                                                                   <  1:409844/251‑1 (MQ=255)

                                                                                     gATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GGCGACCAGCAGCGGAGCGACGGCAGCGAGGTTAAGATCGGGAATCAACCCTTTCGGGCGATGGTTAGCGTTTGGCGGGCGACGACAGTTAAACCAATCGTGCACCACCGGCCAGG‑‑GATCCAGAGA‑CG‑GTGTTTAGGCCCATGCCTCGGGCCGCGCCGGGAGGAGTTCGTTCTGGCTGGCGTTgg                                                                                                                                  >  2:635549/1‑250 (MQ=255)

                                                                                            tGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCgc                                                                                                                           >  1:153267/1‑250 (MQ=255)

                                                                                                 gggaaaGCACTCCATGATTGAGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACCGCAGCGGAGCGTCGGGAGCGAGGTTAAGATCGGAAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGGGCGACGCACGTTAAACCAGTCGAGCAACACCGGC‑CAG‑CACTCCAGAGG‑CG‑GGTTTTGGGCCCATGCTTCGGTCAGCGCCGGAAGCAGATGTTTCTGGCTGGCGTTGATTTgggctttcc                                                                                                                      >  2:284207/4‑243 (MQ=255)

                                                                                                    aaaGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGACGTTAAGAACGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑AG‑GATTTTTGGCCCATGCTTCGGTCAACACCGGCAGTAATTGTTTctggctgcggtgtatttgcgctttctggt                                                                                                                   >  2:211955/1‑229 (MQ=255)

                                                                                                     aaGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGGAGCGAGGTTAAGATCGGTAATCACCCCTTTCCGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTc                                                                                                                  >  1:465166/1‑251 (MQ=255)

                                                                                                      aGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTcc                                                                                                                 <  1:459904/251‑1 (MQ=255)

                                                                                                       gCACTCAATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACG‑GCCTTACAGCAGAGGAGCGTCGGGAGGGAGGTTAAGATCGTGAATCCACCCATTCAGGCGTTGGGAAGCGCTTGAGCAGCGAAGACCGTTAAACCACTCGAGCACCCACGGC‑AGG‑CGAGCCGAAGA‑CGCGATTTT‑GGCCCATGCTTAGGTCAGGCCCGGAAGCACTTGTTTTTGGATGGCGTTGCtttaccttatcgcttccc                                                                                                                >  2:207602/1‑236 (MQ=255)

                                                                                                           cccATGATTGCGCGGGCGCCAAGGGCGGTGGCAGCAA‑CAAACCCGCCGAACAGCAGCGGAGCGTCGGTAGCGATGTTAAGAGCGGTAATCACCCCTTTCATGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATg                                                                                                            <  2:627713/250‑1 (MQ=255)

                                                                                                            ccATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATg                                                                                                            <  1:702619/250‑1 (MQ=255)

                                                                                                             cATGATTGCGCGGGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTGTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCt                                                                                                          <  1:648466/251‑1 (MQ=255)

                                                                                                                gATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTggg                                                                                                       >  1:141816/1‑251 (MQ=255)

                                                                                                                  ggggtggggtgctaaaGGGGGTGGAAGCAACAAAACC‑GCCGAACAGCAGCGGAGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGGTGGTTAGCGGTTGGCGAGCGACGACCGTGAAACCAGGCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTGGCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGggcg                                                                                                     <  2:705767/238‑1 (MQ=255)

                                                                                                                          cccccAAAGCCGGTGGCAGCAACCAAACC‑GCCGAACTGCAGCGGAGCGTCGGTAGCGATTTTAAGATCGTTAATCTCCCCTTTCAGGCGTTGGTTAGCGTTTGGCGATCGACGACCGTTAAACCAGTCGAGCTCCACCGTC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGTCTGGCGTTGATTGGCGCTTTCAGTTCCTGATGCTGGGCGTCAAGCTg                                                                                             <  2:273981/248‑1 (MQ=255)

                                                                                                                           cgcCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGt                                                                                            >  1:34418/1‑251 (MQ=255)

                                                                                                                           cgcCAAAGGCGGTGGCAGCAATCAAACC‑GACGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑GGATCCAGCGA‑AG‑GATTTTTGGCCCATTCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTTGCTGGGGTTGCTTTACGCTTTACGTTCCGGGTGATGGGCGGCAAACTGt                                                                                            >  2:294310/1‑251 (MQ=255)

                                                                                                                                aaGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTcc                                                                                         >  1:642657/1‑249 (MQ=255)

                                                                                                                                     ggTGGCTGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGAACCCCCGGC‑AGG‑TGATCCAGAGA‑AG‑GTTTTTTGGCCCATGCTTCGGTCAGCACCGGAAGTAGTTGTTTTTGGCTGGCGTTGATTTTCGCTTTCAGTTCCGGATGCTGGGCGGTAAGCTGTTCCAGTGGcc                                                                                  >  2:700501/1‑251 (MQ=255)

                                                                                                                                           agcaATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCTGTCAGCGCCGGAAGCCGTTGTTTCTTGCTGGCGTTGGTTTGCGCTTTCAGTTCCGGATTCTGGGCGGCAAGCTGTTCCACGGGCCAGctgg                                                                            >  2:144349/1‑248 (MQ=255)

                                                                                                                                              aaccAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGTCAGCCGAGTa                                                                         <  1:345656/248‑1 (MQ=255)

                                                                                                                                               attaaaCC‑GCCGAACAGCAGCGGAGCGTCGGTATCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGGTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTAc                                                                        <  1:425036/248‑1 (MQ=255)

                                                                                                                                                acAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGATCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTAcg                                                                       <  2:656515/250‑1 (MQ=255)

                                                                                                                                                acAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTAcg                                                                       <  2:642657/250‑1 (MQ=255)

                                                                                                                                                   aaCC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTAcgcg                                                                     >  1:85687/1‑250 (MQ=255)

                                                                                                                                                         ccgAAGAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGAccgaa                                                               <  2:167687/251‑4 (MQ=255)

                                                                                                                                                             aCAGCAGCGGAGCGTCGGTAGCGAGGTTAATATCGGTAATCACCCCTTTCAGGCGTTTGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAg                                                           <  1:519601/251‑1 (MQ=255)

                                                                                                                                                             aCAGCAGCGGAGCGTCGGGAGCGAAGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGGTAGCGTTTTGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGG‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAg                                                           <  1:676057/251‑1 (MQ=255)

                                                                                                                                                               agcagcGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACAAGTCGAGAACCACCGGC‑AGG‑TGATCAAGCGC‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCTCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGAGGCAAGACGTTCCAGCGGACAGCTGCGTAcgccaacgaacaaggc                                                         >  2:413000/1‑238 (MQ=255)

                                                                                                                                                                 cagcGGAGCGTCGGTAGCGAGGGTAAGATCGGTAATCACCCCTGTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCAGGCTGCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGt                                                       <  1:681734/251‑1 (MQ=255)

                                                                                                                                                                  agcGGAGCGTCGGTAGCGAGGTTACGATCGGTAATCACCTCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGACGAGCACCACCGGC‑AGG‑TGATCAAGAGT‑CG‑GGTTTTTGGCCCATGCTTCGGTCAGCGCCGGACGCAGTTGCTTCTGGCTGGCGTTGCTTTGCGCTTTCCGTTCCGGATGCTGGGCGGCAAGCTGTTACAGCGGCCAGCCCGGTAGGCGAccctaccacgctt                                                       >  2:348168/1‑239 (MQ=255)

                                                                                                                                                                    gggAGCGTCGGGAGCGAGGTTAAGCACGGTAATCACCCCTTTCAGGCGTGGGTTTGCGGTGGGCGAGCGACGACCGTTAAACCAGTCGGGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGTAAGCAGGTGTTTCTGGCTGGCGTTGATTTGCGCTTGCAGTTCCGGATGCTGGGCGGTAAGCTGTTCCAGCGGCCAGCTGATTACGCGACCGAACCAGGCGTAGa                                                    <  2:705534/250‑1 (MQ=255)

                                                                                                                                                                    cGGAGCGACGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCTTGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCCACCGAACCAGGCggtg                                                     >  2:257437/1‑247 (MQ=255)

                                                                                                                                                                       aGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTACACCAGTCGAGCACCACCGGC‑CGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCCGTTCCGAGTGCTGGGCGGCACGCTGTTCACGCGGCCAGCTGAGTACGCGACCGAACCAcgcgcatgtat                                                 >  2:339805/1‑244 (MQ=255)

                                                                                                                                                                          gTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGaacgagaccgggggagatatcac                                              >  2:1309/1‑229 (MQ=255)

                                                                                                                                                                            cGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCAcc                                             >  1:343929/1‑250 (MQ=255)

                                                                                                                                                                              gTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAg                                                                                                                                                         >  1:496555/1‑140 (MQ=255)

                                                                                                                                                                              gTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAg                                                                                                                                                         <  2:496555/140‑1 (MQ=255)

                                                                                                                                                                                aGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCATAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACC‑AAACg                                       <  1:307546/251‑1 (MQ=255)

                                                                                                                                                                                 gCGAGGTTAAGATCGGTAATCACCCCTTTCCGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGCTTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGTGTACGCGCCCGAACCAGGCGTAGATA‑CACCCAAACGc                                      >  2:317827/1‑251 (MQ=255)

                                                                                                                                                                                    aGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACC‑AAACGCCg                                    >  1:527355/1‑250 (MQ=255)

                                                                                                                                                                                                  aaTCCCCCCTTCCAGGCGTTGGTTAGCGTTTGGCGAGCGACGAACGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGTTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGAGGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAACAGGCGTAGATATCCCC‑AAACGCCGATTGGCCTGCTTCCa                     <  2:193195/251‑1 (MQ=255)

                                                                                                                                                                                                  aaTCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCt                                                                                                                                                                          >  1:357248/1‑103 (MQ=255)

                                                                                                                                                                                                  aaTCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCt                                                                                                                                                                          <  2:357248/103‑1 (MQ=255)

                                                                                                                                                                                                            tcccggctttGGTTAGCGTTTGGCGAGTGCCGACCGGTAAACCCGTCGAGCCCCCCCGGC‑AGG‑TGCTCCACATC‑CG‑GATTTCTGGCCCATGCTTCGCTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGTGCGGCAAGCTGTTCCAGCGGCCAGCTTAGTACTCGACCTACCCAGCCGTAGATATCGCC‑AAACGCCTATTGGCCTGCCTCCAGCCCGATa             <  2:234265/241‑1 (MQ=255)

                                                                                                                                                                                                              cAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACC‑AAACGCCGATTGGCCTGCTTCCAGACCGATAAAt          <  1:130048/250‑1 (MQ=255)

                                                                                                                                                                                                               aGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTCGATATCACC‑AAACGCCGATTGGCCTTCTTCCCGACCGATAAATcc        >  1:154631/1‑251 (MQ=255)

                                                                                                                                                                                                                ggCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACC‑AAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCa       <  2:601289/251‑1 (MQ=255)

                                                                                                                                                                                                                ggCGTGGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTTTTTCTGGTGGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTTGGCGGCAAGCTGTTCCAGCGGCCAGCGGAGTACGCGACCGAACCAGGCGTAGATATCACC‑AAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCa       <  2:659009/251‑1 (MQ=255)

                                                                                                                                                                                                                      ggTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACC‑AAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGcac  <  1:317827/250‑1 (MQ=255)

GGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACC‑GCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGC‑AGG‑TGATCCAGAGA‑CG‑GATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACC‑AAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCAC  >  NC_000913/68614‑69076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`