Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,476	A→G	C525R (TGC→CGC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,476	0	A	G	97.9%	275.7 / ‑6.6	97	C525R (TGC→CGC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (55/40);  total (56/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

CATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGCTCGCTGCACGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTC  >  NC_000913/68229‑68722                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                         cATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGATTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCgg                                                                                                                                                                                                                                                       >  1:481816/1‑251 (MQ=255)     aacaacttGCTTGGCATGTTGGGTGCCCTGACGCAGGGTTTCCGGGTCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGGG‑CCTGTTCGCTGCGCGGtggc                                                                                                                                                                                                                                                   <  2:587491/244‑4 (MQ=255)       caccgTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCCTAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTTGG‑CCTGTTCGCTGCGCGGTTGc                                                                                                                                                                                                                                                   >  2:500450/4‑249 (MQ=255)           gTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGtt                                                                                                                                                                                                                                            <  1:144392/251‑1 (MQ=255)             gCTCGGCATGTTGGGGGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATAGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGgttt                                                                                                                                                                                                                                          >  2:524634/1‑251 (MQ=255)              cTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGACAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTtc                                                                                                                                                                                                                                         >  1:336932/1‑251 (MQ=255)                 ggCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTctct                                                                                                                                                                                                                                      >  1:92118/1‑251 (MQ=255)                 ggCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTctct                                                                                                                                                                                                                                      >  1:263202/1‑251 (MQ=255)                    aTGTTGGGTGTCCTGGCGTAGGGTGTCCGGGACATACAGATGCTGGCTGCCAATGTCAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGTTGATAGCGGCGAGAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTctctgcc                                                                                                                                                                                                                                   <  2:112434/251‑4 (MQ=255)                       ttGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTT‑CCTGCCCGCTGCACGGTTGCCG‑GGTTTTCTCTAccccc                                                                                                                                                                                                                                >  2:339537/1‑248 (MQ=255)                         gggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGGTGCAG‑GGTTTTCTCCACCGCACt                                                                                                                                                                                                                              >  2:387174/1‑251 (MQ=255)                         gggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCCGCGCGGTTGACG‑GGTTTTCTCTACCGccct                                                                                                                                                                                                                              >  2:571402/1‑248 (MQ=255)                                  gACGCAGGGTTTCCGTGCCATACAGTTGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAATATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGTGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGC‑Attttt                                                                                                                                                                                                                    <  1:26626/251‑1 (MQ=255)                                  gACGCAGGGTTTCCGGGGCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTAGCAAAAATCGTCATTATCGTGTCCGTATAGAGTTGCAACGGCCTGGTCAGCCTGTGCCGGGGCGAAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCAGCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CTTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCAtt                                                                                                                                                                                                                       <  1:650715/249‑1 (MQ=255)                                          gTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTgc‑tggg                                                                                                                                                                                                            <  2:98827/251‑3 (MQ=255)                                              ccGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGAGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGCTAAAGCTGTTAAAATCGTTGTG‑CCTGTTCGCTGCGGGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCCTTTTTTcc‑tgggctgg                                                                                                                                                                                                        >  2:307546/1‑250 (MQ=255)                                                 ggCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATgg                                                                                                                                                                                                     <  1:547767/251‑1 (MQ=255)                                                 ggCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CAATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATgg                                                                                                                                                                                                     >  2:164948/1‑251 (MQ=255)                                                  gCCAGACAGAAGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGACCTTATAGAGACGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGCAGATAGGGTTGT‑TCGGCGCTCATAGC‑CCCTTGCTGATAGCGGAGATGAAGCAGTTCAAAGAGTTGTG‑CCTGTTCGCTGCGCGGTTG‑AGCGGCTTTCTCTACCGCCCTGTCCATTTTTTGC‑TGGGCTGATggg                                                                                                                                                                                                    >  2:681224/1‑251 (MQ=255)                                                      tACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATCAAGCTGTTCAAAGCGATGGG‑CCTGTTCGCTGCGCGGGTGGAG‑GGTTTTTGATACCGCACTGGCCCtttttttcggggcgggtgggatgg                                                                                                                                                                                                 >  2:386081/1‑233 (MQ=255)                                                       aacgatgtgggtggCCAATGACAAAC‑CACACTCCAAAATTATCAAAAATCGACATTGTCGTGTCCTGATAGAGTCGCAACGGTCTGGGCAGCCTGGGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCGTTGCTTATAGCGGCGATAAAGCTGTTCAAAGCTTTGTG‑CCTTTTCTCTGCGCGGTTGCAG‑GGTTTGCTCTCCCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGt                                                                                                                                                                                                <  2:288117/237‑1 (MQ=255)                                                        cAGATGCTGGATGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTATG‑CCTGTTCGCTGCGAGGTTGCAG‑GGTTTTCTCAACCGCACTGGCCATTTTTTGCCTG‑GCTGATGGGATGTCt                                                                                                                                                                                              >  1:495046/1‑251 (MQ=255)                                                            tGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGt                                                                                                                                                                                          >  1:157040/1‑251 (MQ=255)                                                               tggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCa                                                                                                                                                                                                                                                  >  1:251327/1‑194 (MQ=255)                                                               tggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCa                                                                                                                                                                                                                                                  <  2:251327/194‑1 (MQ=255)                                                               tggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGcc                                                                                                                                                                                       >  1:19769/1‑250 (MQ=255)                                                                ggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCAc                                                                                                                                                                                      >  1:358672/1‑251 (MQ=255)                                                                ggctgCCAATGA‑AAACACACACTTCATACTTATCAAAAATCGTCATTATCGTGTCCTTCTAGAGTCGCAACGGCCTGGGCAGCCTGTACCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATTGCGGCTGTAAAGCTGTTGACAGCGTTGTG‑CCTGTTCGCTGCGCGTCTGCCG‑GGTTTTCTCTCCCGCCCTGGCCCTTTTTTGC‑TGGGCTGCTGGGGTGTCCGCGAGCAc                                                                                                                                                                                      >  2:430646/1‑251 (MQ=255)                                                                 gctgCCAAGTACAAAC‑CACACTGCATAATTATCAAAAATCGTCATTATCGTGGCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGGTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACt                                                                                                                                                                                     <  2:701571/251‑1 (MQ=255)                                                                     ccAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGTCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGGTCAAAGCGTTGTG‑CCTTTTCGCTTCGCGGTTGCAG‑GGTTTTCTCTACCCCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCCCTTTcc                                                                                                                                                                                 >  2:558344/1‑250 (MQ=255)                                                                         tGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCgcggc                                                                                                                                                                             >  1:579101/1‑251 (MQ=255)                                                                                  cacaCTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgc                                                                                                                                                                       >  1:477061/1‑249 (MQ=255)                                                                                  cacaCTTCATAATAACCAAAAAGCGTCATTAGCGTGTCCGTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGAGGAAGTTGGAAGATAG‑GTTGTGTCGGCGCTCATCGC‑CCAGTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTGCGCGTCGCGGTTGCAG‑GGTTTTCTCTACCGCATTGGCCATTTTTTGC‑GGGGCTGATGGGAGGTCGGCGTGCACTTTCGCGGCGACGgctg                                                                                                                                                                     <  2:336932/251‑3 (MQ=255)                                                                                   acacTTCATAATTATCAAAAATCGTCATTATCGTATCCTTATAGAGTAGCAACGGCCTGAGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGA‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGAGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTACTCTTACGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgcagc                                                                                                                                                                    >  2:687167/1‑251 (MQ=255)                                                                                     acTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgcagca                                                                                                                                                                   >  1:110070/1‑250 (MQ=255)                                                                                     acTTCATAATTATAAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCAGTGCCGGGGCGGAAGTTGGAAGAAAGTATTGT‑TCGGCGCTCCTCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑ACTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCGACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGGTGGCTACGTGCACTTTCGCAGCGACGgcagca                                                                                                                                                                   >  2:519601/1‑250 (MQ=255)                                                                                                aTCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATcgccgcc                                                                                                                                                       >  1:705767/1‑250 (MQ=255)                                                                                                    aaaaTCGTCAGTACCGTTGCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGGGCCGGGGCGGAAGTTGGAAGAGAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCGGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCGGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTACGCGGCGACGGCAGCAAAAATCGCCGCACCGa                                                                                                                                                   <  2:546609/251‑1 (MQ=255)                                                                                                      aaTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAgc                                                                                                                                                 <  1:248055/251‑1 (MQ=255)                                                                                                          gTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGcac                                                                                                                                             >  1:672452/1‑251 (MQ=255)                                                                                                          gTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGACGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGCTGGGATGTCTGCGTGCACTTTCCCGGCGACGGCAGAAAAGATCGCCGCACCGCGCGcac                                                                                                                                             >  2:504639/1‑251 (MQ=255)                                                                                                           tCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGCAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCCCCGAGCGcac                                                                                                                                             >  2:189573/1‑250 (MQ=255)                                                                                                            cagtATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAg                                                                                                                                           <  1:266284/248‑1 (MQ=255)                                                                                                            cATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGcac                                                                                                                                             >  1:324636/1‑249 (MQ=255)                                                                                                              ttATCGTGTCCTTATCGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGCTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGCTAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGACCTTTCGCGGCGACGGCCGCCAAAATCGCCGCACCgcgcgccaagcc                                                                                                                                         >  2:409844/1‑245 (MQ=255)                                                                                                                aTCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACt                                                                                                                                       <  2:110070/251‑1 (MQ=255)                                                                                                                  cGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCGCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTgg                                                                                                                                     <  1:27833/251‑1 (MQ=255)                                                                                                                   gtgtCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGt                                                                                                                                    >  1:295721/1‑251 (MQ=255)                                                                                                                    tgaccTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCACAAATCGCCGCACCGAGCGCACAGCACTGGTc                                                                                                                                   >  1:310917/4‑251 (MQ=255)                                                                                                                          taaagagcagtaaCGGCCGGGGCAGCCTGTGCCGTGGGGGAAGTGGGAAGATATTGTTGT‑TCGGCGCTCATCTC‑CCTTTGCGGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTT‑CCTGGTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGGCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCATTGGTCagag                                                                                                                               <  2:290240/238‑1 (MQ=255)                                                                                                                                gccGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGTCAACAAt                                                                                                                       <  2:295721/249‑1 (MQ=255)                                                                                                                                gTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTgc‑tgggct                                                                                                                                                                                                          <  1:49758/168‑1 (MQ=255)                                                                                                                                gTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCCGCACAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAAt                                                                                                                       >  2:68970/1‑251 (MQ=255)                                                                                                                                gTCGCAACGGCCTGGGCAGCCAGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGCTCGCGGCGATAAAGCTGGTCAAAGCGTTGTG‑CCTGGTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTgc‑tgggct                                                                                                                                                                                                          >  2:49758/1‑168 (MQ=255)                                                                                                                                       cGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGc                                                                                                                <  1:304688/251‑1 (MQ=255)                                                                                                                                         gCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGCTAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATTTCTGCGTGCACTTTCACGGCGACGGCCGCAAAAATCGCCGCCCCGATCGCACAGCACTGGTCAGAGGCAACAATTGGCAGCgg                                                                                                              >  2:262238/1‑251 (MQ=255)                                                                                                                                         gCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGCGGCAACAATTTGCAGCgg                                                                                                              >  1:595093/1‑251 (MQ=255)                                                                                                                                         gCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCgg                                                                                                              <  1:246424/251‑1 (MQ=255)                                                                                                                                             gggCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGCTGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGGACAGCCCTGGTCCGAGGCAACAATTTGCAGCGGGCgg                                                                                                          >  2:164812/1‑250 (MQ=255)                                                                                                                                              ggCAGCCTGGGCCGGGGCGGAAGTTGGAGGATAGTGTTGT‑TCGGCGCTAATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGCTCGCTGCACGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGAt                                                                                                         <  2:221641/251‑1 (MQ=255)                                                                                                                                                   ccTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGc                                                                                                    <  1:227395/251‑1 (MQ=255)                                                                                                                                                      gtgCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCAc                                                                                                  <  1:351115/250‑1 (MQ=255)                                                                                                                                                        gCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTc                                                                                               <  1:211914/251‑1 (MQ=255)                                                                                                                                                         ccggtgctgaatttGGAAGATAGGGTTGT‑CCGGTGC‑CATTGCTCCATTGCTGTTAGCGGCGGTAAAGCTGTTCAAAGCGTTGTG‑CCCGTTCGCTGGGCGGTTGCAG‑GGGTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCg                                                                                              <  2:10155/239‑1 (MQ=255)                                                                                                                                                         ccGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCg                                                                                              <  1:569321/251‑1 (MQ=255)                                                                                                                                                                      tGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCCCTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGCGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCCCGTCGCAGCAGGCCTGcc                                                                                 >  2:243295/1‑250 (MQ=255)                                                                                                                                                                      tGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGc                                                                                  >  1:14981/1‑250 (MQ=255)                                                                                                                                                                      gggAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGGG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCa                                                                                 <  2:36149/250‑1 (MQ=255)                                                                                                                                                                          aGATAGTGTTGT‑TCGGCGCGTATCGC‑CCCTTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCGGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAAt                                                                             <  2:26631/251‑1 (MQ=255)                                                                                                                                                                           gATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATg                                                                            <  1:243295/251‑1 (MQ=255)                                                                                                                                                                            aTAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGa                                                                           <  1:456171/251‑1 (MQ=255)                                                                                                                                                                                     t‑tCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCCGAGGCAACAATTTGCAGCGGTCGATTCCGCACGTCGCAGCAGGCCTGCATAAAGCCCTGGtttt                                                                  >  2:631849/1‑251 (MQ=255)                                                                                                                                                                                      gctggtccTCATCGC‑CCAGTGCTGATTTCGGTGATAAAGCTTTTCAAAGCGT‑GTGGTCTTGTCGCTGCGCGGTTGCAG‑GGTTTGCTCTACCGCACCGGCCATTTGTTGC‑TGGGCTGATGGGATGTCTGCGCGCGCTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTGGCAGCGGTCCATTCAGCTCGTCGCAGCCGGCCTTCATAATCACCTGGtttg                                                                  <  2:286590/244‑2 (MQ=255)                                                                                                                                                                                         ggCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTcc                                                               >  1:311112/1‑251 (MQ=255)                                                                                                                                                                                           cgcTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCcgc                                                             <  2:263202/251‑1 (MQ=255)                                                                                                                                                                                             cTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCcgcgc                                                           >  1:167687/1‑251 (MQ=255)                                                                                                                                                                                                aTCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGCGCGCACAGCACt                                                                                                                                       >  1:419953/1‑172 (MQ=255)                                                                                                                                                                                                   gC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgcc                                                     <  1:387174/251‑1 (MQ=255)                                                                                                                                                                                                   gC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgcc                                                     <  1:224368/251‑1 (MQ=255)                                                                                                                                                                                                           gCTGATAGCGGCGTTAAAGCTGTTCAAAG‑GGTGTGGCCTGGTCGCTGCGTTGTTGCAG‑GGTTTTCGCTCCCCCACTGGCCATGTTTTGC‑TGGGCTGATGGGATGTCGGCGTGCACGTTCGCGGCGACGGAAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAg                                               <  2:358672/250‑1 (MQ=255)                                                                                                                                                                                                            cTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAgcg                                             >  2:116735/1‑249 (MQ=255)                                                                                                                                                                                                               aTAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTATTTCCGCGCGATGCCGccccgcgccc                                          >  2:487565/1‑244 (MQ=255)                                                                                                                                                                                                                           aaGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTACGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGAGAGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGCTTTTCCGCGCGATGCCGCCAAGCGCCATCACGttattg                               >  1:171085/1‑249 (MQ=255)                                                                                                                                                                                                                             gCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTGTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACg                            <  1:412314/251‑1 (MQ=255)                                                                                                                                                                                                                                gTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGGCCAGCGCCAGCACGTTATTGACGGc                          <  1:204904/250‑1 (MQ=255)                                                                                                                                                                                                                                      aGCGTTGTG‑CCTGTTCGCTGCGCGGTTTCAG‑GGTTTTCTCTACCGCCCTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATcccc                   <  2:477061/251‑1 (MQ=255)                                                                                                                                                                                                                                           tgtg‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATc              >  1:1664/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccTGTTCGATGCGCGGTTGCAG‑GGTTTTCTATACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGt           >  1:273981/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ctgttCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTa          >  1:21821/5‑250 (MQ=255)                                                                                                                                                                                                                                                 ctgttCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTa          >  1:367658/5‑250 (MQ=255)                                                                                                                                                                                                                                                   gttCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTaa         >  1:385278/3‑249 (MQ=255)                                                                                                                                                                                                                                                     tCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGcc     >  1:234265/1‑250 (MQ=255)                                                                                                                                                                                                                                                      cGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCa     <  1:524634/250‑1 (MQ=255)                                                                                                                                                                                                                                                      cGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCAc    >  1:441995/1‑251 (MQ=255)                                                                                                                                                                                                                                                      cGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATGCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCAc    <  2:395521/251‑1 (MQ=255)                                                                                                                                                                                                                                                       gCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACt   >  1:627713/1‑251 (MQ=255)                                                                                                                                                                                                                                                        cTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTc  >  1:29845/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                         CATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGCTCGCTGCACGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTC  >  NC_000913/68229‑68722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑