Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,468	C→T	E527E (GAG→GAA)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,468	0	C	T	95.6%	282.6 / NA	93	E527E (GAG→GAA)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (50/37);  minor base G (1/3);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGCTCGCTGCACGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGT  >  NC_000913/68222‑68713                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        ttCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGc                                                                                                                                                                                                                                                     >  1:263589/1‑251 (MQ=255)        cATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGATTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCgg                                                                                                                                                                                                                                              >  1:481816/1‑251 (MQ=255)            aacaacttGCTTGGCATGTTGGGTGCCCTGACGCAGGGTTTCCGGGTCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGGG‑CCTGTTCGCTGCGCGGtggc                                                                                                                                                                                                                                          <  2:587491/244‑4 (MQ=255)              caccgTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCCTAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTTGG‑CCTGTTCGCTGCGCGGTTGc                                                                                                                                                                                                                                          >  2:500450/4‑249 (MQ=255)                  gTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGtt                                                                                                                                                                                                                                   <  1:144392/251‑1 (MQ=255)                    gCTCGGCATGTTGGGGGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATAGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGgttt                                                                                                                                                                                                                                 >  2:524634/1‑251 (MQ=255)                     cTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGACAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTtc                                                                                                                                                                                                                                >  1:336932/1‑251 (MQ=255)                        ggCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTctct                                                                                                                                                                                                                             >  1:92118/1‑251 (MQ=255)                        ggCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTctct                                                                                                                                                                                                                             >  1:263202/1‑251 (MQ=255)                           aTGTTGGGTGTCCTGGCGTAGGGTGTCCGGGACATACAGATGCTGGCTGCCAATGTCAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGTTGATAGCGGCGAGAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTctctgcc                                                                                                                                                                                                                          <  2:112434/251‑4 (MQ=255)                             gtggggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCt                                                                                                                                                                                                                                                    <  2:410248/221‑1 (MQ=255)                             gTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCt                                                                                                                                                                                                                                                    >  1:410248/1‑224 (MQ=255)                              ttGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTT‑CCTGCCCGCTGCACGGTTGCCG‑GGTTTTCTCTAccccc                                                                                                                                                                                                                       >  2:339537/1‑248 (MQ=255)                                gggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGGTGCAG‑GGTTTTCTCCACCGCACt                                                                                                                                                                                                                     >  2:387174/1‑251 (MQ=255)                                gggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCCGCGCGGTTGACG‑GGTTTTCTCTACCGccct                                                                                                                                                                                                                     >  2:571402/1‑248 (MQ=255)                                         gACGCAGGGTTTCCGTGCCATACAGTTGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAATATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGTGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGC‑Attttt                                                                                                                                                                                                           <  1:26626/251‑1 (MQ=255)                                         gACGCAGGGTTTCCGGGGCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTAGCAAAAATCGTCATTATCGTGTCCGTATAGAGTTGCAACGGCCTGGTCAGCCTGTGCCGGGGCGAAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCAGCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CTTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCAtt                                                                                                                                                                                                              <  1:650715/249‑1 (MQ=255)                                                 gTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTgc‑tggg                                                                                                                                                                                                   <  2:98827/251‑3 (MQ=255)                                                     ccGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGAGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGCTAAAGCTGTTAAAATCGTTGTG‑CCTGTTCGCTGCGGGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCCTTTTTTcc‑tgggctgg                                                                                                                                                                                               >  2:307546/1‑250 (MQ=255)                                                        ggCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATgg                                                                                                                                                                                            <  1:547767/251‑1 (MQ=255)                                                        ggCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CAATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATgg                                                                                                                                                                                            >  2:164948/1‑251 (MQ=255)                                                         gCCAGACAGAAGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGACCTTATAGAGACGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGCAGATAGGGTTGT‑TCGGCGCTCATAGC‑CCCTTGCTGATAGCGGAGATGAAGCAGTTCAAAGAGTTGTG‑CCTGTTCGCTGCGCGGTTG‑AGCGGCTTTCTCTACCGCCCTGTCCATTTTTTGC‑TGGGCTGATggg                                                                                                                                                                                           >  2:681224/1‑251 (MQ=255)                                                             tACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATCAAGCTGTTCAAAGCGATGGG‑CCTGTTCGCTGCGCGGGTGGAG‑GGTTTTTGATACCGCACTGGCCCtttttttcggggcgggtgggatgg                                                                                                                                                                                        >  2:386081/1‑233 (MQ=255)                                                              aacgatgtgggtggCCAATGACAAAC‑CACACTCCAAAATTATCAAAAATCGACATTGTCGTGTCCTGATAGAGTCGCAACGGTCTGGGCAGCCTGGGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCGTTGCTTATAGCGGCGATAAAGCTGTTCAAAGCTTTGTG‑CCTTTTCTCTGCGCGGTTGCAG‑GGTTTGCTCTCCCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGt                                                                                                                                                                                       <  2:288117/237‑1 (MQ=255)                                                               cAGATGCTGGATGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTATG‑CCTGTTCGCTGCGAGGTTGCAG‑GGTTTTCTCAACCGCACTGGCCATTTTTTGCCTG‑GCTGATGGGATGTCt                                                                                                                                                                                     >  1:495046/1‑251 (MQ=255)                                                                   tGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGt                                                                                                                                                                                 >  1:157040/1‑251 (MQ=255)                                                                      tggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCa                                                                                                                                                                                                                                         >  1:251327/1‑194 (MQ=255)                                                                      tggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCa                                                                                                                                                                                                                                         <  2:251327/194‑1 (MQ=255)                                                                      tggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGcc                                                                                                                                                                              >  1:19769/1‑250 (MQ=255)                                                                       ggctgCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCAc                                                                                                                                                                             >  1:358672/1‑251 (MQ=255)                                                                       ggctgCCAATGA‑AAACACACACTTCATACTTATCAAAAATCGTCATTATCGTGTCCTTCTAGAGTCGCAACGGCCTGGGCAGCCTGTACCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATTGCGGCTGTAAAGCTGTTGACAGCGTTGTG‑CCTGTTCGCTGCGCGTCTGCCG‑GGTTTTCTCTCCCGCCCTGGCCCTTTTTTGC‑TGGGCTGCTGGGGTGTCCGCGAGCAc                                                                                                                                                                             >  2:430646/1‑251 (MQ=255)                                                                        gctgCCAAGTACAAAC‑CACACTGCATAATTATCAAAAATCGTCATTATCGTGGCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGGTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACt                                                                                                                                                                            <  2:701571/251‑1 (MQ=255)                                                                            ccAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGTCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGGTCAAAGCGTTGTG‑CCTTTTCGCTTCGCGGTTGCAG‑GGTTTTCTCTACCCCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCCCTTTcc                                                                                                                                                                        >  2:558344/1‑250 (MQ=255)                                                                                tGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCgcggc                                                                                                                                                                    >  1:579101/1‑251 (MQ=255)                                                                                         cacaCTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgc                                                                                                                                                              >  1:477061/1‑249 (MQ=255)                                                                                         cacaCTTCATAATAACCAAAAAGCGTCATTAGCGTGTCCGTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGAGGAAGTTGGAAGATAG‑GTTGTGTCGGCGCTCATCGC‑CCAGTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTGCGCGTCGCGGTTGCAG‑GGTTTTCTCTACCGCATTGGCCATTTTTTGC‑GGGGCTGATGGGAGGTCGGCGTGCACTTTCGCGGCGACGgctg                                                                                                                                                            <  2:336932/251‑3 (MQ=255)                                                                                          acacTTCATAATTATCAAAAATCGTCATTATCGTATCCTTATAGAGTAGCAACGGCCTGAGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGA‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGAGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTACTCTTACGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgcagc                                                                                                                                                           >  2:687167/1‑251 (MQ=255)                                                                                            acTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgcagca                                                                                                                                                          >  1:110070/1‑250 (MQ=255)                                                                                            acTTCATAATTATAAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCAGTGCCGGGGCGGAAGTTGGAAGAAAGTATTGT‑TCGGCGCTCCTCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑ACTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCGACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGGTGGCTACGTGCACTTTCGCAGCGACGgcagca                                                                                                                                                          >  2:519601/1‑250 (MQ=255)                                                                                                       aTCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATcgccgcc                                                                                                                                              >  1:705767/1‑250 (MQ=255)                                                                                                         cAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACTGCCTGGGCAGCCTGTGCCGGGTCGGAATTTGGAAGATATTGTTGT‑TCGGCGCTCATCGC‑CCATGGCTGATAGCTGCGATAAGGCTGTTCAAAGCGTGGTG‑CCTGTTCGCTGcgcg                                                                                                                                                                                                                                               <  2:301170/154‑4 (MQ=255)                                                                                                         cAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGcgcg                                                                                                                                                                                                                                               >  1:301170/1‑151 (MQ=255)                                                                                                           aaaaTCGTCAGTACCGTTGCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGGGCCGGGGCGGAAGTTGGAAGAGAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCGGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCGGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTACGCGGCGACGGCAGCAAAAATCGCCGCACCGa                                                                                                                                          <  2:546609/251‑1 (MQ=255)                                                                                                             aaTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAgc                                                                                                                                        <  1:248055/251‑1 (MQ=255)                                                                                                                 gTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGcac                                                                                                                                    >  1:672452/1‑251 (MQ=255)                                                                                                                 gTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGACGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGCTGGGATGTCTGCGTGCACTTTCCCGGCGACGGCAGAAAAGATCGCCGCACCGCGCGcac                                                                                                                                    >  2:504639/1‑251 (MQ=255)                                                                                                                  tCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGCAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCCCCGAGCGcac                                                                                                                                    >  2:189573/1‑250 (MQ=255)                                                                                                                   cagtATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAg                                                                                                                                  <  1:266284/248‑1 (MQ=255)                                                                                                                   cATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGcac                                                                                                                                    >  1:324636/1‑249 (MQ=255)                                                                                                                     ttATCGTGTCCTTATCGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGCTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGCTAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGACCTTTCGCGGCGACGGCCGCCAAAATCGCCGCACCgcgcgccaagcc                                                                                                                                >  2:409844/1‑245 (MQ=255)                                                                                                                       aTCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACt                                                                                                                              <  2:110070/251‑1 (MQ=255)                                                                                                                         cGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCGCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTgg                                                                                                                            <  1:27833/251‑1 (MQ=255)                                                                                                                          gtgtCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGt                                                                                                                           >  1:295721/1‑251 (MQ=255)                                                                                                                           tgaccTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCACAAATCGCCGCACCGAGCGCACAGCACTGGTc                                                                                                                          >  1:310917/4‑251 (MQ=255)                                                                                                                                 taaagagcagtaaCGGCCGGGGCAGCCTGTGCCGTGGGGGAAGTGGGAAGATATTGTTGT‑TCGGCGCTCATCTC‑CCTTTGCGGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTT‑CCTGGTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGGCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCATTGGTCagag                                                                                                                      <  2:290240/238‑1 (MQ=255)                                                                                                                                       gccGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGTCAACAAt                                                                                                              <  2:295721/249‑1 (MQ=255)                                                                                                                                       gTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTgc‑tgggct                                                                                                                                                                                                 <  1:49758/168‑1 (MQ=255)                                                                                                                                       gTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCCGCACAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAAt                                                                                                              >  2:68970/1‑251 (MQ=255)                                                                                                                                       gTCGCAACGGCCTGGGCAGCCAGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGCTCGCGGCGATAAAGCTGGTCAAAGCGTTGTG‑CCTGGTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTgc‑tgggct                                                                                                                                                                                                 >  2:49758/1‑168 (MQ=255)                                                                                                                                              cGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGc                                                                                                       <  1:304688/251‑1 (MQ=255)                                                                                                                                                gCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGCTAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATTTCTGCGTGCACTTTCACGGCGACGGCCGCAAAAATCGCCGCCCCGATCGCACAGCACTGGTCAGAGGCAACAATTGGCAGCgg                                                                                                     >  2:262238/1‑251 (MQ=255)                                                                                                                                                gCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGCGGCAACAATTTGCAGCgg                                                                                                     >  1:595093/1‑251 (MQ=255)                                                                                                                                                gCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCgg                                                                                                     <  1:246424/251‑1 (MQ=255)                                                                                                                                                    gggCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGCTGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGGACAGCCCTGGTCCGAGGCAACAATTTGCAGCGGGCgg                                                                                                 >  2:164812/1‑250 (MQ=255)                                                                                                                                                     ggCAGCCTGGGCCGGGGCGGAAGTTGGAGGATAGTGTTGT‑TCGGCGCTAATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGCTCGCTGCACGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGAt                                                                                                <  2:221641/251‑1 (MQ=255)                                                                                                                                                          ccTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGc                                                                                           <  1:227395/251‑1 (MQ=255)                                                                                                                                                             gtgCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCAc                                                                                         <  1:351115/250‑1 (MQ=255)                                                                                                                                                               gCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTc                                                                                      <  1:211914/251‑1 (MQ=255)                                                                                                                                                                ccggtgctgaatttGGAAGATAGGGTTGT‑CCGGTGC‑CATTGCTCCATTGCTGTTAGCGGCGGTAAAGCTGTTCAAAGCGTTGTG‑CCCGTTCGCTGGGCGGTTGCAG‑GGGTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCg                                                                                     <  2:10155/239‑1 (MQ=255)                                                                                                                                                                ccGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCg                                                                                     <  1:569321/251‑1 (MQ=255)                                                                                                                                                                             tGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCCCTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGCGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCCCGTCGCAGCAGGCCTGcc                                                                        >  2:243295/1‑250 (MQ=255)                                                                                                                                                                             tGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGc                                                                         >  1:14981/1‑250 (MQ=255)                                                                                                                                                                             gggAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGGG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCa                                                                        <  2:36149/250‑1 (MQ=255)                                                                                                                                                                                 aGATAGTGTTGT‑TCGGCGCGTATCGC‑CCCTTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCGGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAAt                                                                    <  2:26631/251‑1 (MQ=255)                                                                                                                                                                                  gATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATg                                                                   <  1:243295/251‑1 (MQ=255)                                                                                                                                                                                   aTAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGa                                                                  <  1:456171/251‑1 (MQ=255)                                                                                                                                                                                            t‑tCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCCGAGGCAACAATTTGCAGCGGTCGATTCCGCACGTCGCAGCAGGCCTGCATAAAGCCCTGGtttt                                                         >  2:631849/1‑251 (MQ=255)                                                                                                                                                                                             gctggtccTCATCGC‑CCAGTGCTGATTTCGGTGATAAAGCTTTTCAAAGCGT‑GTGGTCTTGTCGCTGCGCGGTTGCAG‑GGTTTGCTCTACCGCACCGGCCATTTGTTGC‑TGGGCTGATGGGATGTCTGCGCGCGCTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTGGCAGCGGTCCATTCAGCTCGTCGCAGCCGGCCTTCATAATCACCTGGtttg                                                         <  2:286590/244‑2 (MQ=255)                                                                                                                                                                                                ggCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTcc                                                      >  1:311112/1‑251 (MQ=255)                                                                                                                                                                                                  cgcTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCcgc                                                    <  2:263202/251‑1 (MQ=255)                                                                                                                                                                                                    cTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCcgcgc                                                  >  1:167687/1‑251 (MQ=255)                                                                                                                                                                                                       aTCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGCGCGCACAGCACt                                                                                                                              >  1:419953/1‑172 (MQ=255)                                                                                                                                                                                                          gC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgcc                                            <  1:387174/251‑1 (MQ=255)                                                                                                                                                                                                          gC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgcc                                            <  1:224368/251‑1 (MQ=255)                                                                                                                                                                                                                  gCTGATAGCGGCGTTAAAGCTGTTCAAAG‑GGTGTGGCCTGGTCGCTGCGTTGTTGCAG‑GGTTTTCGCTCCCCCACTGGCCATGTTTTGC‑TGGGCTGATGGGATGTCGGCGTGCACGTTCGCGGCGACGGAAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAg                                      <  2:358672/250‑1 (MQ=255)                                                                                                                                                                                                                   cTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAgcg                                    >  2:116735/1‑249 (MQ=255)                                                                                                                                                                                                                      aTAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTATTTCCGCGCGATGCCGccccgcgccc                                 >  2:487565/1‑244 (MQ=255)                                                                                                                                                                                                                                  aaGCTGTTCAAAGCGTTGTG‑CCTGTTCGCTACGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGAGAGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGCTTTTCCGCGCGATGCCGCCAAGCGCCATCACGttattg                      >  1:171085/1‑249 (MQ=255)                                                                                                                                                                                                                                    gCTGTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTGTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACg                   <  1:412314/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTTCAAAGCGTTGTG‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGGCCAGCGCCAGCACGTTATTGACGGc                 <  1:204904/250‑1 (MQ=255)                                                                                                                                                                                                                                             aGCGTTGTG‑CCTGTTCGCTGCGCGGTTTCAG‑GGTTTTCTCTACCGCCCTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATcccc          <  2:477061/251‑1 (MQ=255)                                                                                                                                                                                                                                                  tgtg‑CCTGTTCGCTGCGCGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATc     >  1:1664/1‑251 (MQ=255)                                                                                                                                                                                                                                                       ccTGTTCGATGCGCGGTTGCAG‑GGTTTTCTATACCGCACTGGCCATTTTTTGC‑TGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGt  >  1:273981/1‑250 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        TTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAC‑CACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGT‑TCGGCGCTCATCGC‑CCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTG‑CCTGCTCGCTGCACGGTTGCAG‑GGTTTTCTCTACCGCACTGGCCATTTTTTGC‑TGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGT  >  NC_000913/68222‑68713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑