Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,834	G→A	V168V (GTC→GTT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,834	0	G	A	98.5%	208.6 / ‑4.2	67	V168V (GTC→GTT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (46/20);  total (47/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

TGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTT  >  NC_000913/67601‑68079                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       tGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATcc                                                                                                                                                                                                                                        >  1:155385/1‑250 (MQ=255)    aTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACg                                                                                                                                                                                                                                             >  1:399680/1‑242 (MQ=255)            tgCAGGCGTCATGTTGGAGCAGCTTTCGTCCTCTGCGCCCAGCGCTTTAACACCGCCTTCGCTGGTGGAGTCCCCCACCTGCACCAGATCGCCACCCGCCC‑AGTTATTGAGGGAAAACCCGATTTTGATCTGGGCGACAATTTTACCCCCACCGTGG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATa                                                                                                                                                                                                                             <  1:701615/249‑1 (MQ=255)             gCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGAGTTAACATCGCCGTCGCTGATGGCGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCCCGCATGTTATCGCCAAAACGGCCGACTTTCAGATGACGGGTATCCTATTTAGAAACCGCCTGACGCATCCAGGAGCCGATa                                                                                                                                                                                                                             >  2:82696/1‑249 (MQ=255)              cAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGc                                                                                                                                                                                                                          >  1:686438/1‑251 (MQ=255)                  cGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGCATCCTGTTTCGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCAt                                                                                                                                                                                                                      >  2:559144/1‑251 (MQ=255)                   gTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATg                                                                                                                                                                                                                     >  1:523037/1‑251 (MQ=255)                    cctgggTGTAGCAGTTTTCTTACTCACCGCCCAGCGCGTTACCACCGCCGTCGTGGAGGGTGTCCCCCCCCGGCCCAAGA‑CCCCAACCCCCCCAGTTATTGGCGGAAAAACCAAATTTGATTGGTGCGGCATTTTTACCCCCACCGTTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATgg                                                                                                                                                                                                                    <  1:488486/247‑1 (MQ=255)                      aTGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACAGCCTGACGCATCCAGGAGCCGATACGCTCATGGGc                                                                                                                                                                                                                  >  2:10377/1‑251 (MQ=255)                             agcagcTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAACCCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGttat                                                                                                                                                                                                           >  2:534571/1‑249 (MQ=255)                                agcTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATcc                                                                                                                                                                                                        >  1:625651/1‑251 (MQ=255)                                      cGTACTCATAAACCAACGCGTTAACATCGCCTTCGCTGCTGGAGTTCACCACCTGCACAAGAACGCCAACCGCCC‑AGGTATTGACGGAGAAACCGCACTTGATCTGAGCGGCAACTTTCTCACCATCGGCG‑ACCGCCACTTCACGCAGGTGATCGCCAGAACGGCAGCCTTTCAGATGACGGGTATCCTGTTTAGAACCCGCCTGACGCATCCAGGAGCCGATACGCACAGGGGATTGTTTagccgccc                                                                                                                                                                                                    >  2:105493/1‑242 (MQ=255)                                         aCTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTg                                                                                                                                                                                                >  1:136147/1‑250 (MQ=255)                                           tCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGAcc                                                                                                                                                                                             >  1:532561/1‑251 (MQ=255)                                                  ccAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAcg                                                                                                                                                                                      >  1:257020/1‑250 (MQ=255)                                                      cgcgTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGCAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAACCGGCAGACTTTCAGATGCCGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCAGATCCGCTCATGGGCTTGTTTATCCTGCCAGTGGCCGGTaccgacgg                                                                                                                                                                                  >  2:342914/1‑244 (MQ=255)                                                       gcgTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGc                                                                                                                                                                                 >  1:698340/1‑251 (MQ=255)                                                         gTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCAt                                                                                                                                                                               >  1:686016/1‑251 (MQ=255)                                                          ttAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAACACGGCAGCCTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGAAACGACGGCATg                                                                                                                                                                              >  2:531437/1‑251 (MQ=255)                                                            aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCg                                                                                                                                                                                            >  1:530722/1‑235 (MQ=255)                                                                 cGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTg                                                                                                                                                                        >  1:300909/1‑250 (MQ=255)                                                                         cTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcg                                                                                                                                                               >  1:391452/1‑251 (MQ=255)                                                                          tGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                              >  1:529608/1‑251 (MQ=255)                                                                                  ttCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGCTGACGGGTATCCTGTTTAGAAACCGCCTGACGCa                                                                                                                                                                                                                                           >  2:353683/1‑166 (MQ=255)                                                                                          cTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCACCTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAACCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGGCCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCg                                                                                                                                               >  1:478874/1‑250 (MQ=255)                                                                                          cTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCg                                                                                                                                               >  1:581523/1‑250 (MQ=255)                                                                                                cAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGCCCGGTAACGACGGCATGTTGCTGACGCATAccgatcgggagagcgtcgtggcggg                                                                                                                                        >  2:490554/1‑227 (MQ=255)                                                                                                          aCCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtg                                                                                                                              >  1:504977/1‑251 (MQ=255)                                                                                                            cGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGACACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgtg                                                                                                                            >  2:337602/1‑251 (MQ=255)                                                                                                                                        cTTGATCTGAGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGTCGCATACGCGCGCCAATGAAGCCGAAATCGCGACCGCCATGTGCAGTCTGGGTCCGGTTCATAAAgacc                                                                                                  >  2:215187/1‑247 (MQ=255)                                                                                                                                            gtcggggtgcgacatctatacacgtCGGTGCACCGCCACTCCACGCATGTTACCGCCAAACCGGCAGACTTTTAGATGTCGGGTATCCTGTTTAGAACCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTAGCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACGCGTGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCata                                                                                               <  2:686016/226‑1 (MQ=255)                                                                                                                                                   gcgccaCTTTAACCCCAACGGTGGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑t                                                                                      <  1:337602/246‑1 (MQ=255)                                                                                                                                                   cGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCAt                                                                                                                                                                                                                      >  2:364753/1‑123 (MQ=255)                                                                                                                                                      cAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGCCCATATCGATAC‑TGTcc                                                                                  >  1:382516/1‑251 (MQ=255)                                                                                                                                                           ttATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACgg                                                                             >  1:280154/1‑251 (MQ=255)                                                                                                                                                           ttATCACCATCGGAG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGATAC‑TGTCCCACgg                                                                             >  2:709493/1‑251 (MQ=255)                                                                                                                                                             atcaccATCGGTG‑ACCGCCACTTCACGCATGTTAACGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCATGGCGAATCCAGGAGACGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACTCGCGCCAATGAAGCCTAAATCGCGACCGGCATGGGCGGTGTGGGTCAGGTTCATAAAGTCCATAGTGATAC‑TGGCCCCCGGcg                                                                           >  2:76282/5‑250 (MQ=255)                                                                                                                                                               gcacctggggtaaCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAg                                                                          <  1:314996/239‑1 (MQ=255)                                                                                                                                                                  cATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGcgcc                                                                      >  1:10155/1‑251 (MQ=255)                                                                                                                                                                     ggtgaatccgcccttacacccATGTTAGCGCCAAAACGGCAGACTTTCAGATGACGGTTATCCTGTTTAGAACCCGCCTGACGCATCCAGGAGCCGATCCGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCCATAC‑TGTCCCACGGCAGcgccgc                                                                    <  2:8114/231‑1 (MQ=255)                                                                                                                                                                     cGGTG‑ACCGCCACTTCACGAATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTACCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTCGGGTTCATACAGTCCATATCGATAC‑TGTCCCACGGccgcgccgcg                                                                   >  2:76445/1‑251 (MQ=255)                                                                                                                                                                         g‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGAAGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCGGGTTCAGGTTCCTAACGTCCATAACGA‑ACATGTCCAACGGCAGCGCCGCGTTgc                                                               >  2:520812/1‑250 (MQ=255)                                                                                                                                                                            ccGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAAc                                                             >  1:560672/1‑251 (MQ=255)                                                                                                                                                                                    tCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGggtg                                                     >  2:640599/1‑251 (MQ=255)                                                                                                                                                                                     cACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGt                                                                                                                                                                             >  1:697234/1‑131 (MQ=255)                                                                                                                                                                                     cACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCAGCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGt                                                                                                                                                                             <  2:697234/131‑1 (MQ=255)                                                                                                                                                                                         ccgtttATCGACAAAACGGAAGACTTTCAGATGATGGGTAGCCTTTTAAGAAACCGCCGGACGCAGCCAGGAGCCGATACGCTCATGGGCTGGTTTATCCGGCCAGGGACCGGTAACGAGGGCATGTTGCTGACGCATACGCGCGCCATGGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAAtt                                                <  2:686438/247‑1 (MQ=255)                                                                                                                                                                                           ggTTATCGCAAAACCGGCAGACTTTCAAGTGCGGGGTTTCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATCCGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTg                                               <  2:581523/249‑1 (MQ=255)                                                                                                                                                                                              tATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagc                                           >  1:113142/1‑251 (MQ=255)                                                                                                                                                                                              tATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGGTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGAGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCGGCGCCGCGTTGAACTGGGTGTGGAATTgcagc                                           >  2:171739/1‑251 (MQ=255)                                                                                                                                                                                               aTCGCCAACACGGCAGACCTTCAGATGACGGGAACCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGAAACGACGGCATGTTGCTGAAGCGTACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCCGTCTGGTTCAGGTTCATAAAGTCCCTATCGCTAC‑TGACCCCCGGCAGCGCCGCGTTGTACTGGGTGTGGAATTgcaga                                           >  2:54400/1‑249 (MQ=255)                                                                                                                                                                                               aTCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagc                                           <  1:640599/250‑1 (MQ=255)                                                                                                                                                                                                     aacacGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCCTGGGCTTGTTTATCCTGCCCGTGACCGGTAACGACGGCATGTTGCTGACACATACGCGCGCCAATGACGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTCAGGTTCAGAACGTCCAAATCGATAC‑TGTCCCAGGGCAGCGCAGCGCTGAACTGGGCGTGGacatacagctacggtt                                    >  2:273393/5‑236 (MQ=255)                                                                                                                                                                                                        acgcgagacttacgaaTGACGGGTACCCTGTTTAAAACCCCCCTGCCGCATCCAGGAGCCGATACGCGCATGGTCTTGTTTATCCTGCCAGTTACCGGTAACGACGGCATGTTGCTGACGCTTACGCGCGCTAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGTAACGGTttgt                                 <  2:155385/236‑1 (MQ=255)                                                                                                                                                                                                            cAGACTTTCATATGCCGGGTATCTTGTTTAGAAACCGCCTGACGCATCCAGGGGCCGATACGCTAATGGGCTTTTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATTC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAg                             <  2:504977/251‑1 (MQ=255)                                                                                                                                                                                                              gctggtaaggtGACGTGTAGCCGGTTAAGAAACCGCCTGCCGCAGCAAGGACGCGGTATGCTCAGGGGTTGGTTTATCCTGCCAGGGACCGGTAACGACGGCATGTTGCTGATGCGTACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTTAGCa                           <  2:55350/241‑1 (MQ=255)                                                                                                                                                                                                                  ttCAGATGACGGGTACCCTTTTGAGAACCCGCCGGACGCACCCAGTAGCTGATACGTTCAGGGGCTTGTTTATCTTGCCAGTGACCGGTAACGCCGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATgg                        <  2:539311/250‑1 (MQ=255)                                                                                                                                                                                                                        ggACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGTATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGcc                 <  1:625593/250‑1 (MQ=255)                                                                                                                                                                                                                            gggtttcatGTTTAGAATCCGCCTGAAGCATCCCGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTATCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTg             <  2:523037/243‑1 (MQ=255)                                                                                                                                                                                                                            gggTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGaa                                                                                                                                                   <  1:245116/118‑1 (MQ=255)                                                                                                                                                                                                                            gggTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGaa                                                                                                                                                   >  2:245116/1‑118 (MQ=255)                                                                                                                                                                                                                               tATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCACATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc          >  1:66116/1‑251 (MQ=255)                                                                                                                                                                                                                                aTCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAgg                   >  1:562843/1‑241 (MQ=255)                                                                                                                                                                                                                                aTCCTGTTTAGAAACCGCCTGACGCACCCAGGGTCCGATTCGCTCAGGGGCTTGTTTGTCCGGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAgg                   <  2:562843/241‑1 (MQ=255)                                                                                                                                                                                                                                  ccTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCcac       <  1:20964/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGGATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtttt  >  1:493274/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttAGAAACCGCCTGACGCACCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAttt   <  1:76445/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       TGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTT  >  NC_000913/67601‑68079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑