Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,798	T→A	R180R (CGA→CGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,798	0	T	A	94.6%	165.3 / NA	56	R180R (CGA→CGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (44/9);  minor base C (1/2);  total (45/11)
Fisher's exact test for biased strand distribution p-value = 9.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGC  >  NC_000913/67554‑68038                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ccAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGc                                                                                                                                                                                                                                               >  1:109411/1‑249 (MQ=255)        aaTACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGAAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑CCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTc                                                                                                                                                                                                                                       >  2:701615/1‑250 (MQ=255)                            cGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTAACCACCTGCACCAGATAGCCAACCGCCC‑AGGTATTGCCGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACAGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt                                                                                                                                                                                                                   >  1:468504/1‑250 (MQ=255)                                ttgttgttttcccccggggATTTGGTTGGCAGGCGTCTTGGTGTAGCGCCTTCCGTCCCAACCGACCAGCGCGTTAACATCGCGGTCGCGGAGGGATTTCCCCCCCTGCCCAAGACCCCCACCCCCCC‑AGGTATGGACGGAAAAACCGATCTTTATCTGTGGGGAAATTTTACCCCCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaa                                                                                                                                                                                                               <  1:197128/233‑2 (MQ=255)                                                tGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATcc                                                                                                                                                                                               >  1:155385/1‑250 (MQ=255)                                                   aTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACg                                                                                                                                                                                                    >  1:399680/1‑242 (MQ=255)                                                           tgCAGGCGTCATGTTGGAGCAGCTTTCGTCCTCTGCGCCCAGCGCTTTAACACCGCCTTCGCTGGTGGAGTCCCCCACCTGCACCAGATCGCCACCCGCCC‑AGTTATTGAGGGAAAACCCGATTTTGATCTGGGCGACAATTTTACCCCCACCGTGG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATa                                                                                                                                                                                    <  1:701615/249‑1 (MQ=255)                                                            gCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGAGTTAACATCGCCGTCGCTGATGGCGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCCCGCATGTTATCGCCAAAACGGCCGACTTTCAGATGACGGGTATCCTATTTAGAAACCGCCTGACGCATCCAGGAGCCGATa                                                                                                                                                                                    >  2:82696/1‑249 (MQ=255)                                                             cAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGc                                                                                                                                                                                 >  1:686438/1‑251 (MQ=255)                                                                 cGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGCATCCTGTTTCGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCAt                                                                                                                                                                             >  2:559144/1‑251 (MQ=255)                                                                  gTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATg                                                                                                                                                                            >  1:523037/1‑251 (MQ=255)                                                                   cctgggTGTAGCAGTTTTCTTACTCACCGCCCAGCGCGTTACCACCGCCGTCGTGGAGGGTGTCCCCCCCCGGCCCAAGA‑CCCCAACCCCCCCAGTTATTGGCGGAAAAACCAAATTTGATTGGTGCGGCATTTTTACCCCCACCGTTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATgg                                                                                                                                                                           <  1:488486/247‑1 (MQ=255)                                                                     aTGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACAGCCTGACGCATCCAGGAGCCGATACGCTCATGGGc                                                                                                                                                                         >  2:10377/1‑251 (MQ=255)                                                                            agcagcTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAACCCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGttat                                                                                                                                                                  >  2:534571/1‑249 (MQ=255)                                                                               agcTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATcc                                                                                                                                                               >  1:625651/1‑251 (MQ=255)                                                                                     cGTACTCATAAACCAACGCGTTAACATCGCCTTCGCTGCTGGAGTTCACCACCTGCACAAGAACGCCAACCGCCC‑AGGTATTGACGGAGAAACCGCACTTGATCTGAGCGGCAACTTTCTCACCATCGGCG‑ACCGCCACTTCACGCAGGTGATCGCCAGAACGGCAGCCTTTCAGATGACGGGTATCCTGTTTAGAACCCGCCTGACGCATCCAGGAGCCGATACGCACAGGGGATTGTTTagccgccc                                                                                                                                                           >  2:105493/1‑242 (MQ=255)                                                                                        aCTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTg                                                                                                                                                       >  1:136147/1‑250 (MQ=255)                                                                                          tCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGAcc                                                                                                                                                    >  1:532561/1‑251 (MQ=255)                                                                                                 ccAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAcg                                                                                                                                             >  1:257020/1‑250 (MQ=255)                                                                                                     cgcgTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGCAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAACCGGCAGACTTTCAGATGCCGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCAGATCCGCTCATGGGCTTGTTTATCCTGCCAGTGGCCGGTaccgacgg                                                                                                                                         >  2:342914/1‑244 (MQ=255)                                                                                                      gcgTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGc                                                                                                                                        >  1:698340/1‑251 (MQ=255)                                                                                                        gTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCAt                                                                                                                                      >  1:686016/1‑251 (MQ=255)                                                                                                         ttAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAACACGGCAGCCTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGAAACGACGGCATg                                                                                                                                     >  2:531437/1‑251 (MQ=255)                                                                                                           aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCg                                                                                                                                                   >  1:530722/1‑235 (MQ=255)                                                                                                                cGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTg                                                                                                                               >  1:300909/1‑250 (MQ=255)                                                                                                                        cTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcg                                                                                                                      >  1:391452/1‑251 (MQ=255)                                                                                                                         tGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                     >  1:529608/1‑251 (MQ=255)                                                                                                                                 ttCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGCTGACGGGTATCCTGTTTAGAAACCGCCTGACGCa                                                                                                                                                                                                  >  2:353683/1‑166 (MQ=255)                                                                                                                                         cTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCACCTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAACCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGGCCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCg                                                                                                      >  1:478874/1‑250 (MQ=255)                                                                                                                                         cTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCg                                                                                                      >  1:581523/1‑250 (MQ=255)                                                                                                                                               cAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGCCCGGTAACGACGGCATGTTGCTGACGCATAccgatcgggagagcgtcgtggcggg                                                                                               >  2:490554/1‑227 (MQ=255)                                                                                                                                                         aCCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtg                                                                                     >  1:504977/1‑251 (MQ=255)                                                                                                                                                           cGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGACACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgtg                                                                                   >  2:337602/1‑251 (MQ=255)                                                                                                                                                                                       cTTGATCTGAGCGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGTCGCATACGCGCGCCAATGAAGCCGAAATCGCGACCGCCATGTGCAGTCTGGGTCCGGTTCATAAAgacc                                                         >  2:215187/1‑247 (MQ=255)                                                                                                                                                                                           gtcggggtgcgacatctatacacgtCGGTGCACCGCCACTCCACGCATGTTACCGCCAAACCGGCAGACTTTTAGATGTCGGGTATCCTGTTTAGAACCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTAGCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACGCGTGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCata                                                      <  2:686016/226‑1 (MQ=255)                                                                                                                                                                                                  gcgccaCTTTAACCCCAACGGTGGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑t                                             <  1:337602/246‑1 (MQ=255)                                                                                                                                                                                                  cGGCAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCAt                                                                                                                                                                             >  2:364753/1‑123 (MQ=255)                                                                                                                                                                                                     cAACTTTATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGCCCATATCGATAC‑TGTcc                                         >  1:382516/1‑251 (MQ=255)                                                                                                                                                                                                          ttATCACCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACgg                                    >  1:280154/1‑251 (MQ=255)                                                                                                                                                                                                          ttATCACCATCGGAG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGATAC‑TGTCCCACgg                                    >  2:709493/1‑251 (MQ=255)                                                                                                                                                                                                            atcaccATCGGTG‑ACCGCCACTTCACGCATGTTAACGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCATGGCGAATCCAGGAGACGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACTCGCGCCAATGAAGCCTAAATCGCGACCGGCATGGGCGGTGTGGGTCAGGTTCATAAAGTCCATAGTGATAC‑TGGCCCCCGGcg                                  >  2:76282/5‑250 (MQ=255)                                                                                                                                                                                                              gcacctggggtaaCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAg                                 <  1:314996/239‑1 (MQ=255)                                                                                                                                                                                                                 cATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGcgcc                             >  1:10155/1‑251 (MQ=255)                                                                                                                                                                                                                    ggtgaatccgcccttacacccATGTTAGCGCCAAAACGGCAGACTTTCAGATGACGGTTATCCTGTTTAGAACCCGCCTGACGCATCCAGGAGCCGATCCGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCCATAC‑TGTCCCACGGCAGcgccgc                           <  2:8114/231‑1 (MQ=255)                                                                                                                                                                                                                    cGGTG‑ACCGCCACTTCACGAATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTACCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTCGGGTTCATACAGTCCATATCGATAC‑TGTCCCACGGccgcgccgcg                          >  2:76445/1‑251 (MQ=255)                                                                                                                                                                                                                        g‑ACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGAAGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCGGGTTCAGGTTCCTAACGTCCATAACGA‑ACATGTCCAACGGCAGCGCCGCGTTgc                      >  2:520812/1‑250 (MQ=255)                                                                                                                                                                                                                           ccGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAAc                    >  1:560672/1‑251 (MQ=255)                                                                                                                                                                                                                                   tCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGggtg            >  2:640599/1‑251 (MQ=255)                                                                                                                                                                                                                                    cACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGt                                                                                                                                    >  1:697234/1‑131 (MQ=255)                                                                                                                                                                                                                                    cACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCAGCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGt                                                                                                                                    <  2:697234/131‑1 (MQ=255)                                                                                                                                                                                                                                        ccgtttATCGACAAAACGGAAGACTTTCAGATGATGGGTAGCCTTTTAAGAAACCGCCGGACGCAGCCAGGAGCCGATACGCTCATGGGCTGGTTTATCCGGCCAGGGACCGGTAACGAGGGCATGTTGCTGACGCATACGCGCGCCATGGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAAtt       <  2:686438/247‑1 (MQ=255)                                                                                                                                                                                                                                          ggTTATCGCAAAACCGGCAGACTTTCAAGTGCGGGGTTTCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATCCGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTg      <  2:581523/249‑1 (MQ=255)                                                                                                                                                                                                                                             tATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagc  >  1:113142/1‑251 (MQ=255)                                                                                                                                                                                                                                             tATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGGTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGAGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCGGCGCCGCGTTGAACTGGGTGTGGAATTgcagc  >  2:171739/1‑251 (MQ=255)                                                                                                                                                                                                                                              aTCGCCAACACGGCAGACCTTCAGATGACGGGAACCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGAAACGACGGCATGTTGCTGAAGCGTACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCCGTCTGGTTCAGGTTCATAAAGTCCCTATCGCTAC‑TGACCCCCGGCAGCGCCGCGTTGTACTGGGTGTGGAATTgcaga  >  2:54400/1‑249 (MQ=255)                                                                                                                                                                                                                                              aTCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagc  <  1:640599/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCC‑AGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTG‑ACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAC‑TGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGC  >  NC_000913/67554‑68038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑