BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,471	A→T	P289P (CCT→CCA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,471	0	A	T	98.2%	344.2 / NA	111	P289P (CCT→CCA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base A (2/0); major base T (46/60); minor base G (0/2); total (49/62)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGT  >  NC_000913/67224‑67716

cGAGATGGTGACCGTCGTGGATCGGTTACTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCTAGCACCAGGTCATTGCCTGTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCGCCATCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGGAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGa                                                                                                                                                                                                                                                     <  2:656407/251‑1 (MQ=255)

   gaTGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGc                                                                                                                                                                                                                                                  >  1:464917/1‑251 (MQ=255)

   gaTGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGc                                                                                                                                                                                                                                                  <  1:70060/251‑1 (MQ=255)

      gCTGAACGTCGAGGATCGGTTTCTCGTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTg                                                                                                                                                                                                                                                <  2:543186/250‑1 (MQ=255)

         gAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGttt                                                                                                                                                                                                                                             <  1:483272/250‑1 (MQ=255)

         gAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGttt                                                                                                                                                                                                                                             <  1:547107/250‑1 (MQ=255)

         gAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGttt                                                                                                                                                                                                                                             <  1:562724/250‑1 (MQ=255)

         gAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGtt                                                                                                                                                                                                                                              <  1:486977/249‑1 (MQ=255)

          aaCGTCGCGGATCGGTTTCTGTTCTACGGCAATCGACGGGCAGACTTCCAGAATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCTCCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGTCCAGACCTTGAAGCTGTTTCa                                                                                                                                                                                                                                           <  2:227312/251‑1 (MQ=255)

            cGTCGAGGATCGGTTTCTCTTCTACGGCAATCTACGGGCAGACTTCCATCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCTAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATTCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTATCCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGa                                                                                                                                                                                                                                         <  1:670629/251‑1 (MQ=255)

               cGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCg                                                                                                                                                                                                                                      <  1:514711/251‑1 (MQ=255)

                  ggATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCTTGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCCGACGCTGTACGGCCAGCCCTGGGAGCTGGTTCAGACCGTGc                                                                                                                                                                                                                                   >  2:62477/1‑251 (MQ=255)

                       ggTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATc                                                                                                                                                                                                                              <  1:531685/251‑1 (MQ=255)

                       ggTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATc                                                                                                                                                                                                                              >  1:224454/1‑251 (MQ=255)

                            gcccactactgcaaTCGACGGGCAGACTGCCAACATATGGGAGCCGAGCACCAGTGCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGATATCCCCTTCAGGATGCGAAGCAGGGCGGCGGTTTTCCAGCCGCCTTCGCCCGCAAAGCCGTATCCCTGCTGCATCATACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAAACTTCa                                                                                                                                                                                                                          <  2:288936/237‑1 (MQ=255)

                             tcttctACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCaa                                                                                                                                                                                                                         >  1:165335/1‑250 (MQ=255)

                              cttctACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCaa                                                                                                                                                                                                                         >  1:160168/1‑249 (MQ=255)

                              cttctACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCa                                                                                                                                                                                                                          <  1:116081/248‑1 (MQ=255)

                               ttctACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATGCCCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTGCAAAg                                                                                                                                                                                                                       <  2:46218/250‑1 (MQ=255)

                                tctacggcaATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCaaa                                                                                                                                                                                                                        <  1:482941/244‑1 (MQ=255)

                                     ggcaaTCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAggtggtgg                                                                                                                                                                                                                >  1:660688/5‑251 (MQ=255)

                                            gACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCCAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCCGACCGTGCAAATCTTCAAAGGTGGTGGGGAAcgg                                                                                                                                                                                                         >  2:560468/1‑250 (MQ=255)

                                             aCGGGCAGACTTCCAGCAGATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAAcgcg                                                                                                                                                                                                        <  1:467533/251‑1 (MQ=255)

                                                  cagtcTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTGCTCGAAGTGATTGTTGTAGTCCGCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGACGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGGGGTGGTGAACGCGTGGaa                                                                                                                                                                                                   <  2:595015/247‑1 (MQ=255)

                                                  cAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGCAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCACAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCCGACCTGGAAGCTGTTTCAGACCGATCAAATCTTCAAAGGGGGTGGTGAACGGGTGGa                                                                                                                                                                                                    >  2:385982/1‑250 (MQ=255)

                                                                atGGGAGCCGAGCACCAGGTCATTCCCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCa                                                                                                                                                                                     <  1:407891/251‑1 (MQ=255)

                                                                     aGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGAAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCAAGTCGCCTTCGCCCGAAAAGCCGTAACCCTGCTGCATAAGACGATGTACGGCCAGACCCGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAAAGCGGGGGAGCCAACGTGATCCA‑GGaa                                                                                                                                                                                >  2:703675/1‑250 (MQ=255)

                                                                        cGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGc                                                                                                                                                                            >  1:139648/1‑251 (MQ=255)

                                                                        cGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGc                                                                                                                                                                            >  1:263147/1‑251 (MQ=255)

                                                                              ccatgTCATTACCTTGCACGAAGTGATAGGGGTAGTCCTCCAGAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCTGCGGTTGTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTGCAAAGGTGTTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATc                                                                                                                                                                      <  2:125102/247‑1 (MQ=255)

                                                                               cAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGGAAAAGCGTAGAAGCCACCTTGTTCAA‑GGAAACGCTTCATcc                                                                                                                                                                     >  2:390110/1‑251 (MQ=255)

                                                                                aGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCAGTAACCCTGCTGCATCAGACGCTGTAAGGCCCGACCTGGAAGCTGTTTCAGCCCGTGCAAATCTTCAAAGGAGGGGGGGAACGCGTGGAAGCCACCTTGTTCCA‑GCAAACGCttattacc                                                                                                                                                                    >  2:165345/1‑245 (MQ=255)

                                                                                  gccATTGCCTTTCTCTAAGTGATAGGTGTAGTCCTCCATAAAGGAGTTGCCGCCCTGCAGACCGGTTGACATCACCCTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCATACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTCGTGAACGTGTGGAAGACGCCTTGTTCCA‑GTAAACGCGTCATCCCCa                                                                                                                                                                  <  2:126826/249‑1 (MQ=255)

                                                                                     atttccTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGGTGACATCTCCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCTTCCCCAGCt                                                                                                                                                               <  2:373143/247‑1 (MQ=255)

                                                                                      ttACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTc                                                                                                                                                              >  1:286682/1‑251 (MQ=255)

                                                                                            ttCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATa                                                                                                                                                          <  1:411817/249‑1 (MQ=255)

                                                                                            ttCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACACTTCATCCCCAGCTCAATa                                                                                                                                                          <  1:432504/249‑1 (MQ=255)

                                                                                             tctcGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATAcg                                                                                                                                                        <  1:190454/250‑1 (MQ=255)

                                                                                                    gTGATAGGTGTAGTCCGCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCGGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCtt                                                                                                                                                <  2:353336/251‑1 (MQ=255)

                                                                                                      gATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTc                                                                                                                                               <  1:361467/250‑1 (MQ=255)

                                                                                                         aGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGAGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCCATACGCCCCGCTTcccac                                                                                                                                           >  2:488486/1‑248 (MQ=255)

                                                                                                           gtgtAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCTAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCAc                                                                                                                                         >  1:399304/1‑251 (MQ=255)

                                                                                                           gtgtAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCAc                                                                                                                                         <  1:21207/251‑1 (MQ=255)

                                                                                                           gtgtAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGGGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGATTCATCCCCAGCTCAATACGCGCCGCTTCCAGCa                                                                                                                                          >  1:421592/1‑250 (MQ=255)

                                                                                                              tAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGGGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTCCATCCCCAGCTAAATAAGCGCCGCCTACAGCACggt                                                                                                                                      >  2:520251/1‑249 (MQ=255)

                                                                                                               aGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTc                                                                                                                                     >  1:142712/1‑251 (MQ=255)

                                                                                                                gTCCTCCATAAAGGAGGTGCCGCCCTGACGACCGGTTGACCTCACCTTCATGATGCGAAGCAGGTCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTGCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCt                                                                                                                                    <  2:224454/251‑1 (MQ=255)

                                                                                                                    tccATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAACGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGACGCTGTTTCAGAcc                                                                                                                                                                                                                                       >  2:493724/1‑149 (MQ=255)

                                                                                                                     ccATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATAACCTTCATGATGCGAAGCATCGCGGTGGAATTCAAGTCGTCTCCGCCCGCAAAGCCGTAACCCTGCTGCAGCAGACGCTGTACGGCCAGTCCTGGAAGCTGTTTCATACCGTGCAAATCTTCAACGGTGGGGGTGCACTCGTGGAAGCCGCCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTc                                                                                                                                 <  1:662380/249‑1 (MQ=255)

                                                                                                                         aaaGGAGGTGCCGCCCGGACGTCCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTGCCAGTCGCCTTCGCCCGCAATGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAATGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGtttt                                                                                                                            <  2:203982/250‑1 (MQ=255)

                                                                                                                           aggaggTGCCGCCCTGCAGAACGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGGACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCACATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTACTCCCCTGCTCAATACGCGCCGCTTCCAACCAGTTCTGTCGtttttcc                                                                                                                         >  2:279041/1‑249 (MQ=255)

                                                                                                                             gaggTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGcc                                                                                                                       >  2:164334/1‑251 (MQ=255)

                                                                                                                                          gCAGACCGGTTGAAAACACCTTCCAGATCGGAAGCAGGGCGGCGTTTTTCCAGTCGCCTGCGCCCGCAAAGCCGTAACCCTGCGGCCTCAGACGCTGTAGGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGTAAGCTACCTTGTTCCA‑GGAATCGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgtgtt                                                                                                          <  2:263108/251‑2 (MQ=255)

                                                                                                                                                     gACATCACCTTAATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGTTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCAt                                                                                               <  2:238008/251‑1 (MQ=255)

                                                                                                                                                      ccATCTCCTTCATGAAGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTGCGCCGTGGATTTGTGTTGCAGGCGTCATg                                                                                              <  2:399304/250‑1 (MQ=255)

                                                                                                                                                          cACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCGTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATTCGCGCCGCTTCCAGCACGTTCTGTCTTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGgtg                                                                                           <  1:587519/250‑1 (MQ=255)

                                                                                                                                                          cACCGTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCCGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGACCGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGgtgt                                                                                          <  1:699692/251‑1 (MQ=255)

                                                                                                                                                                  tgatgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCtt                                                                                  >  1:460072/1‑251 (MQ=255)

                                                                                                                                                                    atgagAAGCATGGCTGCTGTTTTCCCTTCGCCTGCTGCCGCATAGCAGTAACCCTGCTGCATCACACGCTGTACTGCCTGACCTGGAAGCTGTTTCATACCGTGTAAATCTTCAAAGGGTGTGGTGACCGCGTGGAAGCGTCCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCTTTGTTCGCCGTTGATTTGTGTGTCAGGcttcttggggtatcacctgta                                                                                <  2:479236/247‑21 (MQ=255)

                                                                                                                                                                    atgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACATGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGAGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGAGTAGCAGCTTTc                                                                                >  1:79539/1‑251 (MQ=255)

                                                                                                                                                                         aaGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACt                                                                           <  1:146945/251‑1 (MQ=255)

                                                                                                                                                                          aGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTc                                                                          <  1:236986/251‑1 (MQ=255)

                                                                                                                                                                          aGCAGGGCGGCGGGTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGTCCTGGCAGCTGTTTAAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGGGGAAGCCACCTGGTTCCA‑GGAAACGCTTCATACCCAGCTCAATACGCGCCGCTTCCAGCCAGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTTCAGTCGTCATGGTGTAGCCTCTTTCGTACTc                                                                          >  2:161559/1‑251 (MQ=255)

                                                                                                                                                                              gggcggcggtgttccagacggtgtCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCCCCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCGTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCg                                                                      <  2:385415/228‑1 (MQ=255)

                                                                                                                                                                               ggcggcGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCg                                                                      >  1:245108/1‑250 (MQ=255)

                                                                                                                                                                                       ttttccagcggcttccccccGCAAAGCGTTAACCCGCCTCCACCAGCCGCTGTCCGGCAGGACCGGGAGGTTGTTTCAAACCGTGCAAACCTCAAAAGGTGGGGTTGAACGCGTGGAAGCCCCCTT‑TTCCAAGGAACCGTTTCTTCCCCAGCTAAATCCGCGCGGCTTC‑AGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGcgcg                                                             <  2:139648/233‑1 (MQ=255)

                                                                                                                                                                                          tCCAGTCGCCGTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGTCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGGTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGtt                                                           <  2:258273/250‑1 (MQ=255)

                                                                                                                                                                                           ccAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTaa                                                         <  1:37222/251‑1 (MQ=255)

                                                                                                                                                                                           ccAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTa                                                          >  1:415795/1‑250 (MQ=255)

                                                                                                                                                                                              gTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACAt                                                      >  1:620651/1‑251 (MQ=255)

                                                                                                                                                                                                cGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAACGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCATCTTTCGTACTCATCGACCAGCGCGTTAACAtgg                                                    >  2:502182/1‑249 (MQ=255)

                                                                                                                                                                                                cGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCg                                                    >  1:413884/1‑251 (MQ=255)

                                                                                                                                                                                                cGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTAAGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCg                                                    >  1:588747/1‑251 (MQ=255)

                                                                                                                                                                                                 ccctgcGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGATGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGGTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGc                                                   <  2:464917/246‑1 (MQ=255)

                                                                                                                                                                                                  ccTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCa                                                                                                       >  1:434410/1‑198 (MQ=255)

                                                                                                                                                                                                  ccTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGAGTTGTGTTGCa                                                                                                       <  2:434410/198‑1 (MQ=255)

                                                                                                                                                                                                      cGCCCGCAAGGTCGTAACCCTGCGGCATCAGCCGCTGTACGGCCAGTCCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAATGTGGGGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTc                                               <  2:710408/250‑1 (MQ=255)

                                                                                                                                                                                                        cccGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCt                                            <  2:607654/251‑1 (MQ=255)

                                                                                                                                                                                                              aaGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGt                                                                                                                                  <  2:507478/159‑1 (MQ=255)

                                                                                                                                                                                                                  cGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTc                                  >  1:152808/1‑251 (MQ=255)

                                                                                                                                                                                                                   gTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGAGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGCTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCCCGATAACATCGCCGTCGCTGATgcattta                                  >  2:20964/1‑244 (MQ=255)

                                                                                                                                                                                                                      aCCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTcacca                              <  1:62477/251‑1 (MQ=255)

                                                                                                                                                                                                                       cccTGCTGCATCAGACGCTGTACGGACAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAAAGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTcaccac                             >  1:228523/1‑251 (MQ=255)

                                                                                                                                                                                                                          tgctgcATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTg                          >  1:482550/1‑251 (MQ=255)

                                                                                                                                                                                                                           tcgtcatcaggtgcgtgaCGGCCAGACCGTGAATCTGGTTCAGACTTTGCAAATCTGCAAAGGTGGTGGTGAACGCGTGGAAGCCGTCTTTTTTCT‑GGAAACTCTTCATGCCCAGCTCAATACGCGCCGTTTCCAGCACGTTCTGTCGGTTGTCGCCGTGGATTTGGGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGGTAACAGCGCCGTCGCTTATGGAGCTcacctccg                           <  2:704344/232‑5 (MQ=255)

                                                                                                                                                                                                                              gcATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAc                       <  1:560468/250‑1 (MQ=255)

                                                                                                                                                                                                                                aTCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAcc                      >  1:161711/1‑249 (MQ=255)

                                                                                                                                                                                                                                    gACGCTGTACGGCCAGACCTGGAAGCTGGTTCAGACCGTGCATATCTACAAAGGTGGTGGTGAAAGCGTGGAAGCCGCCTGGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTGCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCg                <  2:269347/251‑1 (MQ=255)

                                                                                                                                                                                                                                     aCGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGAGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCCGATCGc               >  2:708316/1‑251 (MQ=255)

                                                                                                                                                                                                                                      cGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGcc              >  2:556401/1‑251 (MQ=255)

                                                                                                                                                                                                                                        cTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCa             >  1:8114/1‑250 (MQ=255)

                                                                                                                                                                                                                                         tGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAAc           >  1:248847/1‑251 (MQ=255)

                                                                                                                                                                                                                                          gTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCaa            <  1:18783/249‑1 (MQ=255)

                                                                                                                                                                                                                                          gTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAAcc          >  1:310712/1‑251 (MQ=255)

                                                                                                                                                                                                                                             cGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGcc       >  1:656795/1‑251 (MQ=255)

                                                                                                                                                                                                                                               gCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGGCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCa     <  2:142712/251‑1 (MQ=255)

                                                                                                                                                                                                                                                ccAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGccc      >  1:539311/1‑249 (MQ=255)

                                                                                                                                                                                                                                                 cAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCCCCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGGTCTGTCGTTTTGCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAgg   <  2:660688/251‑1 (MQ=255)

                                                                                                                                                                                                                                                 cAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAgg   >  1:367349/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  aGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGt  <  1:604908/251‑1 (MQ=255)

CGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCA‑GGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGT  >  NC_000913/67224‑67716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`