BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,195	G→T	R381R (CGC→CGA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,195	0	G	T	94.2%	389.8 / 1.3	139	R381R (CGC→CGA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (3/5); new base T (64/65); total (69/70)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAA  >  NC_000913/66948‑67438

ggCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCgg                                                                                                                                                                                                                                                  >  1:595015/1‑251 (MQ=255)

ggCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCgg                                                                                                                                                                                                                                                  <  1:432537/251‑1 (MQ=255)

    catagcggtGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGTGCTCCACCAGCGAGGATCCACGCTGAGGAAGCAGTTGGCAGATCCGGTTGCGCTTGCCGCAGCGCATTCGCTACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTACCGATCGGCGATATCAATCAGGTTGGCGACAAGCGCTGGACCGGTTTGGGTAGTGAATATCAGTCGGGCAg                                                                                                                                                                                                                                              <  2:555911/243‑1 (MQ=255)

      tatCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGGTACAGTTAACCAGCAGACGGTAACGATCGCCGAGGTCAATCAGGCTGGCGACAATCGCTGGCCCGGTTTGGGGATTGAAGATCAGGCGGGCAggg                                                                                                                                                                                                                                            >  2:108214/1‑250 (MQ=255)

        gcgtggAGGTGCAGCGCAGGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTGGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTAGTGAAGATCAGTCGGGCAGGAtg                                                                                                                                                                                                                                          <  2:66449/246‑2 (MQ=255)

               ggTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCCGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGATGATCAGACGGGCAGGATCGTCCtt                                                                                                                                                                                                                                    >  1:162335/1‑250 (MQ=255)

                   cagcgCATGGCTGAAGCCGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTGTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTACCCAGACGGCGGTACCACTCCCGGGGATCGATAAGGCTGGCGACAAACGCAGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTACttcacac                                                                                                                                                                                                                               >  2:249466/5‑246 (MQ=255)

                   cagcgCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGGAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCCGGATCGGCCttcaccc                                                                                                                                                                                                                               >  2:504431/5‑246 (MQ=255)

                     gggcaaggCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTAccacca                                                                                                                                                                                                                             <  2:166480/245‑1 (MQ=255)

                               gAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGAt                                                                                                                                                                                                                   <  1:204136/251‑1 (MQ=255)

                               gAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGAt                                                                                                                                                                                                                   <  1:160544/251‑1 (MQ=255)

                                   gcggtagtgTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTg                                                                                                                                                                                                               <  1:572/243‑1 (MQ=255)

                                    cGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCCATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTg                                                                                                                                                                                                               >  2:374255/1‑250 (MQ=255)

                                    cGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGCATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTg                                                                                                                                                                                                               >  1:331106/1‑250 (MQ=255)

                                        aTGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGCTCAATCAGGCTGGCGACAATCGCTGGACCGGGTTTGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGTGATGCTGACCGt                                                                                                                                                                                                          >  2:190454/1‑251 (MQ=255)

                                             gcgcgcCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAg                                                                                                                                                                                                      <  1:504431/250‑1 (MQ=255)

                                                   cagcaGCGAGGATCCACGCTGCGGAAGCAGTTGGCAGGTCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCTAGGATCGGt                                                                                                                                                                                               <  2:129301/248‑1 (MQ=255)

                                                     ccaGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCCCCGGCAGATTCAGCAGGGAGTGAGGAGGTTTCACCGTGTCGCTACAGTTAACAAGCAGGCGGTAACGATCGCCGAGATAAATCAAGCTGGCGACAATCAATGGAACGGTTTGGGTAATGAACATCAGGCGGGCAGGATAGTCCTTAACACCCATACCCAGAAGCTGAACATCGGGGAACGGttt                                                                                                                                                                                             >  2:407891/1‑251 (MQ=255)

                                                        gCGAGGATCCACGCTTCGTAAGCAGTTGGCAGACCCGGTTGCGCTTTCCACAGCGCATTCGCCCCCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTtctc                                                                                                                                                                                          <  1:249466/251‑1 (MQ=255)

                                                         cGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTctct                                                                                                                                                                                         <  1:663195/251‑1 (MQ=255)

                                                            ggATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttct                                                                                                                                                                                      <  1:191507/251‑1 (MQ=255)

                                                             gATCCCCGCTTCGGAAGCAGTGGGCAGACCCGGTTGCGCTTTCCACAGCGCATTCGCCAACGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCAACGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttcta                                                                                                                                                                                     <  2:104578/251‑2 (MQ=255)

                                                               tCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttctacg                                                                                                                                                                                   >  1:689093/1‑248 (MQ=255)

                                                               cccACGCTTCGGAATCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAAGACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttctacg                                                                                                                                                                                   <  1:9541/250‑4 (MQ=255)

                                                                   cGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttatacggca                                                                                                                                                                                >  1:22993/1‑242 (MQ=255)

                                                                     cTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGAAAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATc                                                                                                                                                                             >  1:48996/1‑251 (MQ=255)

                                                                         ggAAGCAGTTGGCAGATACGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATAAAGTTAACCAGCAGACGGTAAAGGTCGCAGAGATCAATCAGTCTGGCGACAATCGGTGGACCGGTTTGGGGATTGAAGATCAGTCGGGCAGGATAGTACTTACCACCAATACCGAGATGATGAAAGTCGGGGAGAGGTTTCtcttcatcggcaaacgacg                                                                                                                                                                         >  2:360152/1‑237 (MQ=255)

                                                                          gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACgg                                                                                                                                                                        <  1:417907/251‑1 (MQ=255)

                                                                           aaGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACggg                                                                                                                                                                       >  1:565057/1‑251 (MQ=255)

                                                                             gCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGGCGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCCATACCGAGATGCTGAACGTCGCGGAACGGTTTCTCTTCTACGGCAATCGACGGGc                                                                                                                                                                      >  2:655671/1‑250 (MQ=255)

                                                                                gTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCTCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTCACCAGCAGACTGTAACGATCGCCGAGATCAATCCGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGCAGATCAGTCGGGCAGGATCCACCTTCCCACCAATACCGAAATGCTGAACGTCGAGGATCGGTTTCTCTTCTTCGGCAATTGCCGGGCAGAc                                                                                                                                                                  >  2:569582/1‑251 (MQ=255)

                                                                                     cAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCa                                                                                                                                                             <  1:374255/251‑1 (MQ=255)

                                                                                     cAGATCCGGTTGCGCTTGCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGCGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCa                                                                                                                                                             <  1:633612/251‑1 (MQ=255)

                                                                                          ccGGTTGCGCTTTCCACAGCGCCTTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGGCAATCGCTGGACCGGTTTGGGTATTGACGATCAGTCGGGCCGGATCGTCCTTACCACCAATACCGAGGTGATGAACGTCGAGGGTCGGGTTCTCTTCTACGGCAATcggcggaccggcttcccgcatt                                                                                                                                                        >  2:531907/1‑235 (MQ=255)

                                                                                          ccGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCat                                                                                                                                                         <  1:308995/250‑1 (MQ=255)

                                                                                          ccGGGTGCGCTTGCCACTGCGCATGCGCCACCGGCCGGTTCGGCAGGGAGGGCGTGGGGTTCACCGTGTCGATACAGTTAATCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTTGACCGGTGTGTGTATTGAAGATCAGTCGGGCAGGATCGTCCTTTCCACCAATACCGAGATGCTGAACGTCGAGGATCGGGTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCat                                                                                                                                                         <  2:380731/250‑1 (MQ=255)

                                                                                           cGGTTGCGCTTTCCACAGCGCATGCGTCACCGGCAGTTTCGGCCGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCatat                                                                                                                                                       <  2:527212/251‑1 (MQ=255)

                                                                                            ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGCGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATg                                                                                                                                                      >  1:174931/1‑251 (MQ=255)

                                                                                                     ttCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCg                                                                                                                                               >  1:472589/1‑249 (MQ=255)

                                                                                                     ttCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGa                                                                                                                                              <  1:474127/250‑1 (MQ=255)

                                                                                                       ccACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGc                                                                                                                                            <  1:569582/250‑1 (MQ=255)

                                                                                                        cacaGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCAc                                                                                                                                          <  1:655671/251‑1 (MQ=255)

                                                                                                         acaGCGCAGTCGCCACCGGCCGTTGCGGCAGGGAGTGCTGTGTTTTCGCCGTGTGGATACAGTTAACCCGCCGACGGTAACGATCGCCGAGAGCAAGCAGGCGGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTGTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGTATATGGGATCCGAGcgcc                                                                                                                                         <  2:127941/251‑4 (MQ=255)

                                                                                                                aTTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCCATCGACGGGCAGACTTCCAGCATATGGGAGCCCAGCACCAGGTCAt                                                                                                                                  >  2:163333/1‑251 (MQ=255)

                                                                                                                  tCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCAtt                                                                                                                                 <  1:167797/250‑1 (MQ=255)

                                                                                                                   cGCCACCGGCATTTTCGGCAGGGATTGCGGTGGTTTCACCGTGTCGATACAGTTAACCAGCAGGCGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGGATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTa                                                                                                                                <  1:286857/250‑1 (MQ=255)

                                                                                                                   cGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTTGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTa                                                                                                                                <  2:484964/250‑1 (MQ=255)

                                                                                                                    gCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGCGCACCAGGTCATTAcc                                                                                                                              >  1:710408/1‑251 (MQ=255)

                                                                                                                     ccACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATg                                                                                                                                                      >  1:698547/1‑226 (MQ=255)

                                                                                                                     ccACCGGCAGTTACGGCAGGGAGTGCGGTGGTTGCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCGGGCGACAATCGCAGGACCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCGACGTCAATCGACGGGCAGACTTCCAGCATATg                                                                                                                                                      <  2:698547/226‑1 (MQ=255)

                                                                                                                         cGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGGCATTACCTTtct                                                                                                                         <  1:603160/251‑1 (MQ=255)

                                                                                                                         cGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTAGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGAATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCCGCATATGGGAGCCGAGCACCAGGTCATTACCTTtct                                                                                                                         >  1:126826/1‑251 (MQ=255)

                                                                                                                            cAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGCCGGGCAGACTTCCAGCATATGGGCGCCGAGCACCAGGTCATTACCTTTCTCg                                                                                                                       >  1:125102/1‑250 (MQ=255)

                                                                                                                                ttCGGCAGGGAGGGCGGTGTTTTCCCCGTGTCGATGCAGGTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATTGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGt                                                                                                                   <  2:630660/250‑1 (MQ=255)

                                                                                                                                   ggCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATa                                                                                                               <  1:116528/251‑1 (MQ=255)

                                                                                                                                       gggAGTGCGGTGTTTTCACCGTGGCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGgtg                                                                                                           <  1:497930/251‑1 (MQ=255)

                                                                                                                                                  gtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTCCTCGaatggttaggtgatgtcctccct                                                                                                >  2:604908/1‑230 (MQ=255)

                                                                                                                                                  ggtctCACCGTGTCGATACTGTGAACCAGCAGACGGTAACGATCGCCAAGATCAATCAGGCGGGCGACAATCGCTGGATCTGTTTGGGTATTGAAGAGCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCGCTTCTACTGCAAACTACGGGCACACGTCCAGCATATGGGAGCCTAGCACCAGGTCATTACCTTTCTCGAAGTGATAgtcgttgacctccat                                                                                                <  2:401792/247‑15 (MQ=255)

                                                                                                                                                       cacaggggcGATACAGTTAACCAGCAGACGGTAACGATCGCGGAGATCAATCAGGCGGGCGACAATCGCTGGTCCGGTTTGGGTATTGAAGATCAGTCTGGCAGGATCGTCCTTACCGCCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCTCCAGGTCATTTCCTTTCTCGAAGTGAGAGGTGTAGTCCTCCATAAagg                                                                                           <  2:657410/243‑1 (MQ=255)

                                                                                                                                                            ggtCGATCCAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCCGTCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGAAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTg                                                                                      <  2:428156/250‑1 (MQ=255)

                                                                                                                                                                 atacAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGAAGGCTCGTCCTTACCACCAATACCGAGATGCTGAACGTAGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGAATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTaccgcc                                                                                 >  1:103998/4‑251 (MQ=255)

                                                                                                                                                                   acAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTTCTCCATAAAGGAGGTGCCGcccg                                                                               <  2:296606/250‑2 (MQ=255)

                                                                                                                                                                    cAGTTAACCAGCAGACGGTAACGATCGCCGAGATCACTCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATCCCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCATCCATCAAGGAGGTGCCGCCCTg                                                                              >  1:288936/1‑251 (MQ=255)

                                                                                                                                                                     aGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGc                                                                             >  1:179960/1‑251 (MQ=255)

                                                                                                                                                                         aaCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGCAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCCATCGACGGGCAGACTTCCAGCATATGGGAGACGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATACAGGAGGGGCCGCCCTGccaa                                                                          >  2:600691/1‑247 (MQ=255)

                                                                                                                                                                         aaCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGAc                                                                         <  1:407059/251‑1 (MQ=255)

                                                                                                                                                                         aaCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGAc                                                                         >  2:190868/1‑251 (MQ=255)

                                                                                                                                                                          aCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGTGGATCGGTTTCTCTTCTACGGCAATCGACGGACAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGCTAGGTGTAGTCCTCCATCAAGGAGGGGCAGCCCTGCAGAcc                                                                        >  2:37222/1‑251 (MQ=255)

                                                                                                                                                                           ccAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCg                                                                       <  1:108214/251‑1 (MQ=255)

                                                                                                                                                                             agcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGCGGTGCCGCCCTGCAGACCGGt                                                                     >  1:238008/4‑251 (MQ=255)

                                                                                                                                                                               caGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCggctg                                                                   >  2:514711/2‑248 (MQ=255)

                                                                                                                                                                                aGACGGTAACGATCTCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTg                                                                   >  1:142729/1‑250 (MQ=255)

                                                                                                                                                                                aGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTg                                                                   >  1:667920/1‑250 (MQ=255)

                                                                                                                                                                                       aaCGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGc                                                                                                                                                           <  2:228849/155‑1 (MQ=255)

                                                                                                                                                                                       aaCGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGc                                                                                                                                                           >  1:228849/1‑155 (MQ=255)

                                                                                                                                                                                                    atcaatcaGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCa                                                                                                                                                          >  2:535039/1‑143 (MQ=255)

                                                                                                                                                                                                    atcaatcaGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCa                                                                                                                                                          <  1:535039/143‑1 (MQ=255)

                                                                                                                                                                                                      caatcagGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAg                                             <  1:531907/250‑1 (MQ=255)

                                                                                                                                                                                                            ggCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGAGCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGACCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCACAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcg                                      <  2:162335/250‑1 (MQ=255)

                                                                                                                                                                                                            aGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGAGCGTCCTTACCGCCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGACGTGATAGGTGTAGTCCTCCATAAAGGGGGTGCCGCCCTGCCGACCGGTTGACATCACCTTCATGATTCGAAGCAGggcg                                      >  1:541017/1‑251 (MQ=255)

                                                                                                                                                                                                              cTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCATTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGCAGTGATAGGTGTAGTCCTCCATACAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggc                                    >  2:361467/1‑251 (MQ=255)

                                                                                                                                                                                                                 gCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggcggt                                 >  1:682960/1‑248 (MQ=255)

                                                                                                                                                                                                                  cGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGtt                                >  2:498733/1‑251 (MQ=255)

                                                                                                                                                                                                                      aaTCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTgccg                                                                                   >  1:329338/1‑196 (MQ=255)

                                                                                                                                                                                                                       aTCGCTGGACCGGTTTGGGAATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCCTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGGGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTcc                            >  2:281478/1‑250 (MQ=255)

                                                                                                                                                                                                                         cGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGGTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGGGCCGCCCTGCATACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGAGGTTTTCCAGt                         >  2:18783/1‑251 (MQ=255)

                                                                                                                                                                                                                          gcttggCCGGTTTGGGTATTTAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGGTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGCGCACCTGGACATTACCTTTCTCGAAGTGATAGGTGTAGGCCTCCATAAAGGAGTTGCCGCCCTGCTGAACGGTTGAAATCACCTTCATGCTTCGAGGCGGGGCGGAGGTTTTACAGTc                        >  2:22423/5‑251 (MQ=255)

                                                                                                                                                                                                                           cTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATACGAAGCAGGGCGGCGGGTTTCCAgacg                       >  2:482941/1‑248 (MQ=255)

                                                                                                                                                                                                                              gaccGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTATACGGCAATCGACGGGCAGACTTCCAGCATAGGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGAGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAAACCGGTTGACAGAACCTTCATGATACGAAGAAGGGCGGCGGGTTTcccgccgcac                    >  2:560212/3‑243 (MQ=255)

                                                                                                                                                                                                                                ccGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAGCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTc                  <  1:252470/251‑1 (MQ=255)

                                                                                                                                                                                                                                 cGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCg                 <  1:521982/251‑1 (MQ=255)

                                                                                                                                                                                                                                     ttGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCg             >  1:645998/1‑251 (MQ=255)

                                                                                                                                                                                                                                      tGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGCCGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGc            >  1:51571/1‑251 (MQ=255)

                                                                                                                                                                                                                                      tGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGc            >  1:373143/1‑251 (MQ=255)

                                                                                                                                                                                                                                      tGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGc            >  1:258273/1‑251 (MQ=255)

                                                                                                                                                                                                                                       gggTATTTAAGCTCAGTCGGGCCGGATTGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAACACCAGGTCATTAACTTTCTCGAGGTGATCGATGTACTACTCCCTAAAGGAGGTGCCGACCTGCAGCCCGGTTGACATCACCTTCATAGTGCGAAACAGCTCTGCAGTTTTCACGTCGCCTTCGCCCGc            >  2:625593/1‑250 (MQ=255)

                                                                                                                                                                                                                                       gggTATTGAAGATCAGTCGGGCAGGCTCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAAACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTCACATTCGCCTTCCCCCGc            >  2:699692/1‑250 (MQ=255)

                                                                                                                                                                                                                                        ggTATTGAAGAGCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCaa          <  1:73815/251‑1 (MQ=255)

                                                                                                                                                                                                                                             tGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTTCCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGcc      <  2:432032/250‑1 (MQ=255)

                                                                                                                                                                                                                                                agctCAGTCGGGCAGGTTCGTCCTTTCCACCAATACCGGGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATTGACGGGCAGACTTTCAGCATATTGGAGTCCAGCCCCAGGTCATTTCCTTTCGTGGAGTGATAGGTGTAGACCTCCATAAAGGATGTGCCGCCCGGTAGTCCGGTTGACATCACTTTCATGATGCGATGCAGGGCGGCGGTGCTCCATTCGCCTTCTGCCGCTAAGCCGTaa  <  2:395886/248‑1 (MQ=255)

GGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAA  >  NC_000913/66948‑67438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`