BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,970	T→C	A456A (GCA→GCG)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,970	0	T	C	97.0%	289.8 / ‑3.9	101	A456A (GCA→GCG)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (53/43); total (57/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

Only 100 of 101 total aligned reads displayed.

GGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTT  >  NC_000913/66725‑67212

ggCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGGGATTTCAATGTCGGG‑CATCTCGGCGAACAGGCGCATATCGTTGAGGGTCAGCGCATg                                                                                                                                                                                                                                                  >  1:637094/1‑251 (MQ=25)

ggCGTTGAATGCCGGATGCGCTTTGCTTAGCCGGCCTACAAAATCGCAGCTTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATg                                                                                                                                                                                                                                                  <  1:699048/251‑1 (MQ=255)

            cGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGGTCCGCGCATCGC‑T‑GAAGACggg                                                                                                                                                                                                                                    >  1:428156/1‑250 (MQ=37)

             ggATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGGACCGGCGCATATCGTTGGGGGTCCGCGCATGGC‑T‑GAAGACggg                                                                                                                                                                                                                                    >  1:71588/1‑249 (MQ=25)

                   gcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTgc                                                                                                                                                                                                                             <  1:31632/251‑1 (MQ=38)

                         cTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAT‑CACCGTGATTTCCATGTCGTG‑CATTTCGGCGAAATGGCGCATATCGTTGAGGGTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGccc                                                                                                                                                                                                                       >  1:234604/1‑250 (MQ=37)

                            aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGCCGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGGTTTTAAT‑CAACGTGTTTTCAATTTC‑TGTCATCTGGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCCTGGC‑TTGCAG‑CGGTCTGGTGCGCGccaccc                                                                                                                                                                                                                    >  2:298902/1‑250 (MQ=19)

                               cGGCCTACAAAATCGCAACGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATTAATACACGGT‑ATTTCAATGTCGTG‑CATCTTGGCGTACTCGCGCATATCGGTGGGGGTCAGCGCATGGC‑T‑GGAGACGGGATTGGGCGGGCCACCcgcg                                                                                                                                                                                                                 >  2:347379/1‑251 (MQ=255)

                                 gCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACAAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGAGTCGGTATCAAA‑TAACGAGATTTCATTATCGTT‑CATTTCGGCGAACTGGCCCCTATAGGTGAGGGTTACGGCATGGC‑T‑GAAGACgggagaggtggtgccacccgca                                                                                                                                                                                                                 >  1:370894/1‑229 (MQ=255)

                                     aCAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTAGTTCCAGCGCAGCGCGTCTTTAAACGCTGGAAGGCGGGTATCGTTATCCCA‑CACCGTGTTTTCCATATAGTG‑CATATCGGCGAATTGGCGCATATCGTTTGGGGTCAGCGCCTGTCAT‑GA‑GACGGTATGGGGGGGGCCACCCGCGAGGATc                                                                                                                                                                                                           >  2:534223/1‑251 (MQ=255)

                                        aaagcGCAGCGTGTAGGCCAGATAAGACGCGGCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATTGTGCGCGCCACCAGCGAGGATCCAc                                                                                                                                                                                                        <  2:301142/247‑1 (MQ=255)

                                        aaaacGCAGCGTGTAGGCCTGATAAGACGCGTAAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCAc                                                                                                                                                                                                        <  2:328889/247‑1 (MQ=255)

                                        aaaTCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCAc                                                                                                                                                                                                        >  1:484964/1‑251 (MQ=255)

                                              cAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCg                                                                                                                                                                                                  <  2:355268/251‑1 (MQ=255)

                                               aGCGTGTAGGCCTGACAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCCTGGC‑T‑GAAGACGGTATGGGGCGCGCCACCAGCGAGGAACCACGCTTCgg                                                                                                                                                                                                 >  1:432032/1‑251 (MQ=255)

                                                  gtgtAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GGAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGGTTCGGa                                                                                                                                                                                                >  1:478823/1‑249 (MQ=255)

                                                  gtgtAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGAATGGTGCGCGCCACCAGCCAGGATCCCCGCTTCGGa                                                                                                                                                                                                >  1:66449/1‑249 (MQ=255)

                                                      aGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGCTTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCCTATCGTTGTGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGGGCGCCCCCAGCGGGGAGCCCCGCTTCGGAAGCAgg                                                                                                                                                                                          >  2:381247/1‑250 (MQ=255)

                                                       ggccttaTAAGACGCGGCAGCGTCGCATCAGTCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGGCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTGCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGtt                                                                                                                                                                                         <  2:238413/245‑1 (MQ=255)

                                                           tgATAAAACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGAATCGTTATCAAT‑CACCGTGGTTTCAATGCAGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCCAGGA‑T‑TAAGACGGTAAGTTTCGCGCCCCCATCGAGGGTCCACGCTTCGGGAGCAGTTGGc                                                                                                                                                                                      >  1:127941/2‑250 (MQ=255)

                                                              tAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGGACTGGCGCATAACGTTGAGGGTCAGCGCATGGC‑T‑GAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGGAGCAGTTGGCAGa                                                                                                                                                                                   >  1:296606/1‑250 (MQ=255)

                                                               aaGACGCGTCAGCGTTGTATCAGGCGTTACACCCCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTGCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTGCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAt                                                                                                                                                                                  <  2:663465/250‑1 (MQ=255)

                                                               aaGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAt                                                                                                                                                                                  <  1:381247/250‑1 (MQ=255)

                                                                aGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATcc                                                                                                                                                                                <  1:671835/251‑1 (MQ=255)

                                                                aGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATcc                                                                                                                                                                                <  2:194814/251‑1 (MQ=255)

                                                                  aCGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCgg                                                                                                                                                                              <  1:264377/251‑1 (MQ=255)

                                                                        cAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTgcg                                                                                                                                                                        <  1:290081/250‑1 (MQ=255)

                                                                           cGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGGG‑CATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAACGCATGGC‑T‑GAAGACGGGATGGGGCGCGCCACCAGGGCAGATCCACGCCTTGGAAGcgggttggagatacgggttcgcttt                                                                                                                                                                     >  2:454739/1‑227 (MQ=255)

                                                                            gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTc                                                                                                                                                                   <  1:181078/251‑1 (MQ=255)

                                                                                              cacctgATGCGACTACTTAGCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTT‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCatc                                                                                                                                                 <  2:285378/246‑3 (MQ=255)

                                                                                               acctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGCG‑CATCTCGGCGGACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GGAGGCCGTATTGGGCCCGCCACCAGAGAGGATCCACGCCTCCGGAGCCGTTGGGAGGTCCGGGTTG‑GCTTTccccaccgccatcgcccccc                                                                                                                                                >  2:625289/5‑233 (MQ=255)

                                                                                                cctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCgg                                                                                                                                               >  1:318767/4‑251 (MQ=255)

                                                                                                cctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCgg                                                                                                                                               <  1:347379/248‑1 (MQ=255)

                                                                                                   gATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCa                                                                                                                                             <  2:637094/250‑1 (MQ=255)

                                                                                                       cgactactgatctacgaAATCCGTAATACACATCGTTCCAGCGCTGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCGCTGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                                                                                                        <  2:622112/238‑1 (MQ=255)

                                                                                                       cgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                                                                                                        <  1:514594/248‑1 (MQ=255)

                                                                                                        gacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGACACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCCCATTCGCCACCGGCAGTTTCg                                                                                                                                       >  1:129301/3‑251 (MQ=255)

                                                                                                        gacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCATTTGGCAGATCCGG‑TTgc                                                                                                                                                                         <  1:559675/215‑1 (MQ=255)

                                                                                                         acTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAA‑CACCGTGATTTCAATGTCGTG‑CATCCCGGCGAACTTGCGCCTATCGTTGAGGTTCAGCGCATGGC‑T‑GCAGACGGTATGGGGCGCGCCACCCGCGAGGCACGCCGCTCCGGAAGCAATTGGCCGGTCCGG‑TTGCGATTTCCACCACGCCTTCGCCACCGGCAggctctg                                                                                                                                      >  2:22628/2‑245 (MQ=255)

                                                                                                                   gacgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGc                                                                                                                            <  1:702729/251‑1 (MQ=255)

                                                                                                                       aaatccGTAATACACTTCGATCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGTTATCGTTATCAAT‑CACCGTGGTTTCAATGTGGTG‑CATCTCGGCGGACTGGCCCATATTGCTGAGGTTTCGCGCACTGC‑T‑GGAGACGGTATGGTGCGCGCCACCCGCGAGGCTCCCCGCCTCGGGAGCAGTTGGCTGGTCCGG‑TGGCGCGTTCCCCAACGCATTCCCCACCCGCaagttagcccgggggggcgggg                                                                                                                        >  2:467533/5‑230 (MQ=255)

                                                                                                                            cGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCCAA‑CACCGTGCTTTCAATGTCGTG‑CCTCTCGGCGAACTGGAGCATATCGTTGAGGTTCAGCGCATGGC‑T‑TAAGACGGTATGGTGCGAGCCACCCGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACCGACCATTCGCCCCCGGCAGCGTCTGCAGGGGGTGCGGGGtttat                                                                                                                   >  2:521982/1‑249 (MQ=255)

                                                                                                                            cGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTg                                                                                                                                                                          <  1:222797/196‑1 (MQ=255)

                                                                                                                            cGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGGTGAGGTTCAGCGCATGGC‑C‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGGTGGCAGATCCGG‑TTg                                                                                                                                                                          >  2:222797/1‑196 (MQ=255)

                                                                                                                            cGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGCTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGCACTGGCGCATATCGTTGAGGTTCAGCGCCTGGC‑T‑GAAGACGGTATGGGGAGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCCCATTCGCCACCGGCAGTTTCGGCCGGGAGTGCgggggtttt                                                                                                                   >  1:46218/1‑244 (MQ=255)

                                                                                                                            cGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAT‑CACCGTGATTTCCATGTCGTG‑CATCTCGGCGAACTGGCGCCAATCGTTGAGGGTCAGCGCCTGGC‑T‑GAAGACGGGATGGGTCGCGCCCCCCGCGGGGATCCCCGCTTCGGAAGCAGTTGGCAGAACCGG‑TTGGGCTTTCCACTGGGCCTTGCCCACCGGGAGTTTCGGGAgggggagtggggttttt                                                                                                                   >  2:282663/1‑237 (MQ=255)

                                                                                                                             gTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGAATTGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGC‑T‑GAAGACGGTATGGGGCGCGCCACCAGCGGGGAACCACGCTTCGGAAGCAGATGGGAGATACGG‑TTGCGCTTTCCACAACGCCTTCGCCCCCGGCAGGTTCGGCAgggggggcgggggttttc                                                                                                                  >  2:497930/1‑236 (MQ=255)

                                                                                                                               aaTACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCAc                                                                                                                 <  1:534223/250‑1 (MQ=255)

                                                                                                                                 tACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CAACGTGATTTCCATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑GTGCGCTTTCCACAGCGCATTCGCCACCGGCAGGTTCGGCAGGGAggtagggggtttcccacg                                                                                                              >  1:395886/1‑234 (MQ=255)

                                                                                                                                        cGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGGATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGGAGGGAGTgggggggtttccacgtgtcggt                                                                                                        >  1:550948/1‑229 (MQ=255)

                                                                                                                                         gTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAA‑AACCGTGATTTCAATGTCGTG‑CATCTCGGCGAAATGGAGCATATCGTTGAGGTTCAGCGCATGTC‑T‑GAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCCCGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCACCACCGGCAGTTTATGCCGGGAGAGCGGTGgtttacccgtggcactac                                                                                                      >  1:407548/1‑237 (MQ=255)

                                                                                                                                          ttCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGAGTGTGTCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCGGTTTCGGCAGGGGGGGCGGGGGTTTCACCGAGTCGCTGc                                                                                                      >  2:204136/1‑250 (MQ=255)

                                                                                                                                              agcgcagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGGTTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCCGCGCATGGC‑T‑GAAGACGGGATGGGGCGCGCCACCAGCGCGGATCCCCGCTTCGGAAGCAGTTGGCAGATCCGG‑GTTCGCTTTCCACAGCGCATTTCCCACCGGCAGGTTCCGCCGGGGGGGCGGGGTTTTCACCTTGTCgcgccagat                                                                                                  >  2:663195/1‑242 (MQ=255)

                                                                                                                                                   agcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGGGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATAGTTGTGGGTCAGAGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCCGTTGGCAGAACCGG‑TTGCGCTTTCCACAGAGCATTAGCACCCGGCAGTTTCGGCAGGGGGGGCGGTGGTTTTACCGTGTCGAAACAGTTAAcc                                                                                              >  1:657410/1‑249 (MQ=255)

                                                                                                                                                     cgcgTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCcac                                                                                                                                                                <  1:189007/181‑1 (MQ=255)

                                                                                                                                                     cgcgTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAgca                                                                                          <  1:292579/251‑3 (MQ=255)

                                                                                                                                                      gcgTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGGTGAGGGTCAGCGCATGGC‑T‑GAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGGAGGGGGGGCGGGGGTTTCACCggggcgataaaggtaaacaga                                                                                           >  1:336907/1‑229 (MQ=255)

                                                                                                                                                        gTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGAc                                                                                       <  1:565098/251‑1 (MQ=255)

                                                                                                                                                        gTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGC‑T‑GAAGACGGGATGGTGCGCGCCACCAGCGAGGGTCCACGCTTCGGGAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCCGCAGGGAGTGCGGTGGTTTTACCGTGTCCGTACAGTTAACCAGCAGa                                                                                        >  1:104578/1‑250 (MQ=255)

                                                                                                                                                        gTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CAACGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGGTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGGTTCGGCAGGGGGAGGGGTGTTTTTCCCGTGTCGATACAGTTAACCAGCAGAc                                                                                       >  1:227312/1‑251 (MQ=255)

                                                                                                                                                          cTTTAAACGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑GTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTGTCGATACAGTTAACCAGCAGACgg                                                                                     >  1:166480/1‑251 (MQ=255)

                                                                                                                                                                aCGCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTTAGCGCATGGC‑T‑GAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGGTTTGGCAGGGAGTGCGGGGTTTTTACCGTTTCGGTACAGGTAACCAACAGACGGTaaagg                                                                               >  1:653375/1‑248 (MQ=255)

                                                                                                                                                                  gCTGGCAGGCGGGTATCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc                                                                             <  1:202882/251‑1 (MQ=255)

                                                                                                                                                                             ggtatCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGCTTTCACCGTGTCGATACAGTTAACCTGCAGACGGTAACGATCGCCGAGatca                                                                   >  1:70243/5‑250 (MQ=255)

                                                                                                                                                                              gtatCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGAGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGTCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTCACCCGCAGACGGGAAAGATCTCCGGGatcaa                                                                  >  2:21207/4‑250 (MQ=255)

                                                                                                                                                                                atCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGgtcaatc                                                                >  1:385415/2‑250 (MQ=255)

                                                                                                                                                                                  cgtaagcaat‑caCCGTGAGTTCAATGTCGTG‑CATCGCGGCGAACTGGCGCATATCGTGGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGatcaatcag                                                              <  2:318767/244‑2 (MQ=255)

                                                                                                                                                                                  cGTTATCAAT‑CACCGTGATTGCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGatcaatcagg                                                             <  1:369377/251‑3 (MQ=255)

                                                                                                                                                                                     tATCAAT‑CACCGTGATTTCAATGTCGGG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTTCGCTTTCCACAGCGCATTCGCCACCGGCAGTTCCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCt                                                           <  1:341586/250‑1 (MQ=255)

                                                                                                                                                                                      atcaat‑caCCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTgg                                                         >  1:205240/1‑251 (MQ=255)

                                                                                                                                                                                        caat‑caCCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCg                                                       <  2:280445/251‑1 (MQ=255)

                                                                                                                                                                                         aat‑caCCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCCGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTCAAGATCTCCGAGATCAATCCGGCTGGCg                                                       >  2:486977/1‑250 (MQ=255)

                                                                                                                                                                                          at‑caCCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGAc                                                     >  1:527212/1‑251 (MQ=255)

                                                                                                                                                                                               ccGTGATTTCAATGTCGGG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATc                                                 <  2:162451/251‑1 (MQ=255)

                                                                                                                                                                                                 gTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCCTTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAAAGATCGCCGCGTTCCATCAGGATGGCGCCAATCGc                                               >  2:119405/1‑251 (MQ=255)

                                                                                                                                                                                                    gattgcaagggcgttcATCTCGGCGAACTGGCGCATATCGTTGGTGTTCATCTCATGGC‑T‑GAAGACGGTATGTTGCGCTCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCTGTGTTTTCTCCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGTGACAATCGCt                                              <  2:671791/235‑1 (MQ=255)

                                                                                                                                                                                                      ttCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                                                                                                        >  1:297667/1‑157 (MQ=255)

                                                                                                                                                                                                      ttCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                                                                                                        <  2:297667/157‑1 (MQ=255)

                                                                                                                                                                                                       tCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATTGCCGAGATCAATCAGGCTGGCGACAATCGCTggac                                          <  1:511851/250‑3 (MQ=255)

                                                                                                                                                                                                         aaTGTCGTG‑CATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTTTCGATACAGTTTACCAGCAGACGGGCACGATCGCCGGGATCCATCCGGCTGGCGACCATCGCTggacagg                                       >  2:166779/1‑246 (MQ=255)

                                                                                                                                                                                                            gTCGTG‑CATCTCGGCGACCTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCCCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGCGTGCGGGGTTTTCACCGAGTCGGTCAAGTTAACAAGCAGACGGGAACGGTCGCCGGGATCAAACAGGCTGGCGACAATAGCTGGGCCGGttt                                    >  2:677786/1‑251 (MQ=255)

                                                                                                                                                                                                            gTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGttt                                    <  1:490953/251‑1 (MQ=255)

                                                                                                                                                                                                            gTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGtt                                     <  1:169781/250‑1 (MQ=255)

                                                                                                                                                                                                             gcGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTg                                   <  1:625289/250‑1 (MQ=255)

                                                                                                                                                                                                              cGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTgg                                  >  1:269347/1‑251 (MQ=255)

                                                                                                                                                                                                                      ctcGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTAGGCAGGGCGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGAACGCCGGGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTgg                           >  2:308995/1‑250 (MQ=255)

                                                                                                                                                                                                                        cGGCGAACTGGCGCATTTCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTagt                             <  2:478823/247‑3 (MQ=255)

                                                                                                                                                                                                                        cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTAtt                             <  1:55003/247‑1 (MQ=255)

                                                                                                                                                                                                                        cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGGTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGa                           <  1:163051/249‑1 (MQ=255)

                                                                                                                                                                                                                         ggCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGa                           >  1:263108/1‑248 (MQ=255)

                                                                                                                                                                                                                          gCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGAt                       <  1:512616/251‑1 (MQ=255)

                                                                                                                                                                                                                           cGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGa                           >  1:568041/1‑246 (MQ=255)

                                                                                                                                                                                                                             aactGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATc                      >  1:204926/4‑249 (MQ=255)

                                                                                                                                                                                                                              actGGCGCATATAGTTGCGGTTCAGCGCATGGA‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCAAAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGAGGAGTTTTCAACGTGTCGCTACAGTTAACACGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACACTCGCTGGACCGGTTTGGGTATTGAAGATCAgt                   >  1:77013/3‑250 (MQ=255)

                                                                                                                                                                                                                                 ggCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCgg                <  1:432537/251‑1 (MQ=255)

                                                                                                                                                                                                                                 ggCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCgg                >  1:595015/1‑251 (MQ=255)

                                                                                                                                                                                                                                     catagcggtGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGTGCTCCACCAGCGAGGATCCACGCTGAGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTGCCGCAGCGCATTCGCTACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTACCGATCGGCGATATCAATCAGGTTGGCGACAAGCGCTGGACCGGTTTGGGTAGTGAATATCAGTCGGGCAg            <  2:555911/243‑1 (MQ=255)

                                                                                                                                                                                                                                       tatCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGGTACAGTTAACCAGCAGACGGTAACGATCGCCGAGGTCAATCAGGCTGGCGACAATCGCTGGCCCGGTTTGGGGATTGAAGATCAGGCGGGCAggg          >  2:108214/1‑250 (MQ=255)

                                                                                                                                                                                                                                         gcgtggAGGTGCAGCGCAGGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TGGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTAGTGAAGATCAGTCGGGCAGGAtg        <  2:66449/246‑2 (MQ=255)

                                                                                                                                                                                                                                                ggTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCCGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGATGATCAGACGGGCAGGATCGTCCtt  >  1:162335/1‑250 (MQ=255)

GGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTT  >  NC_000913/66725‑67212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`