| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,830 | G→A | intergenic (‑280/+5) | araD ← / ← araA | L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,830 | 0 | G | A | 98.6% | 187.7 / NA | 74 | intergenic (‑280/+5) | araD/araA | L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (33/38); minor base C (1/0); total (34/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTG‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCA > NC_000913/66585‑67073 | ccgtccTATAAAATCGCGGAGTGTATGCCTGATAAGACGCGCCAGCGTCGCAGCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGGTGAATGCCGGATGCGCTTTGCTTAGCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCGGCGTCGCATCAGGCGTTACACACCTGATGCGactact < 2:469276/247‑1 (MQ=17) gtcTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAGCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACAACTGATGCGACTACTTAg < 2:393852/249‑1 (MQ=18) ccTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGc > 1:502055/1‑251 (MQ=255) ccAAAACCGCATCGTGCAGGCCCGCTAAGACGCGCCAGCGTAGCATCAGGCGTTGAACGCCGGATGCGCTTTGCTTATCCGGCCTACCAAAGCGGAACGCGTAGGCC‑CGATAAGATGCGTTAGCGTCGCATCAGGCGTTGAATGTCGGATGCGCTTTGTTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAATACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGcga < 1:445710/249‑1 (MQ=21) accGCAGCGGGTAGGCCTGAAAAGACGCGCCGGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGaaat < 2:112382/249‑2 (MQ=18) cgagcttgtggtccggaaaaGACGCGCTATCGGCGCAACATGCGTAGAAGGCCGGGTTCGCTTTGCTTATCCGGCCTTCAAAAACGGACCGCGTAGGCC‑TGATAAGACGCGTCAGCGGCGCATCAGGCGTTGAATGCCGGATGCGCATTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCg < 2:6456/233‑1 (MQ=16) gtgtAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAAGGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATa < 1:360681/251‑1 (MQ=18) gttggCCTGATAAGACGCGCTAGCGGCGCATCAGGCGTTGTATGCCGGTTGCGCTTTGCTTATACGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGGGTCAGCGTCGCATCAGGCGTTTAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCTGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATac < 2:408807/247‑1 (MQ=18) gCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTc < 1:145343/251‑1 (MQ=18) aTAAGACGCGCCAGCGTCGAATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACTCCTGATGCGACTACTGAGCGACGAAATCCGTAATACACTGCGTTcc < 2:640660/251‑1 (MQ=18) gcgcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTTTCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCcagcgcag < 2:429084/251‑1 (MQ=18) cATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGGCTTTaa > 1:555911/1‑250 (MQ=18) aTCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAAc < 1:224452/251‑1 (MQ=18) cAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAACTCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGc > 1:630660/1‑251 (MQ=18) gCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTgg > 1:355268/1‑251 (MQ=18) cGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGc > 1:328889/1‑251 (MQ=18) attccGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCgg < 2:234386/248‑2 (MQ=18) aTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCgg < 1:467564/251‑2 (MQ=18) tccgtattcgcTTTGCTTATCCGGCCTACAAAATCTGAACGCGTAGTCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTTAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGTCTACTTAGCGACGAAATCCGTAATACTCTTCTTTCCAGCGCAGCGCTTCTTTAAACGCTGGCAGGCggg < 1:609674/242‑3 (MQ=18) cGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATAAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCggggt > 2:512529/1‑246 (MQ=18) ggATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCgggtatc < 1:305698/251‑7 (MQ=18) aTGCGCGTTGCTTATCCGGCCTAAAAAATCGGAACGCGTATGCC‑GGTTAAGACGCGCATGCGTCGCTTCTGGCGTTTAATGCCGGATTCTCTTTGCTTTTCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCTTCATCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTATCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGTCAGGCGGGTA‑TCGt < 2:447001/251‑1 (MQ=19) gcgcTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCG‑TCGGCCTGATAAGACGCGTCAGAGTCACATCAGGCGTTACACACCAGATGCGACTACTTAGCGACGAAATCCGAAATACACTACAGTCCAGCGCAGCGCGTCCTTAAACGCaggcaggcaggttgcgtt > 2:116528/1‑240 (MQ=17) gttgtcttagcggggcTACAAAATCGGAACGCGTAGGCCGTG‑TAAGACGCGCCCGCGTCGCATCGGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAACGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCGTCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTGAAACGCTGGCAGGCGGGTA‑TCGTTatca < 2:247892/236‑1 (MQ=18) tATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGCATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAACGTG‑CAGGCCTGGTAAGACGCGTCCGCGTCGCATAAGGCGCTACACACCTGACGCGACGACTTCGCGTCGAAATCCGGAATACGCTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCggggactcgctacaacaccac > 2:169781/1‑231 (MQ=12) tATCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTACTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CAc < 1:234613/249‑1 (MQ=21) aTCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACCCCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAgggggggatagttatccatccccgg > 2:24034/1‑228 (MQ=18) aTCCGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGt < 1:24034/251‑1 (MQ=21) cGGCCTACAAAATCGGAACGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTTTCAAT‑CACCGTggt > 1:295393/1‑249 (MQ=21) gaacgcgTAGGCC‑TGATAAGACGCGCCAGCGTCGCGTCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAACATCGCAGCGTG‑TAGGCCTGATAAGACACGTCAGCGGCGCATCAGACGTTACACACCAGATGCGCCTACTTAGCAACGACATCCGTTATACACTTCGTTCCAGCACAGCGCGTCTTTAACAGCTGGCCGGCGGGGA‑TCGGTATCAAT‑CAccacgagtgtcatgttgtgagt > 2:432537/4‑231 (MQ=12) acgcgTAGACC‑TGATAAGACGCGCCAGCGTCGCATGTGGCGTTGGATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGAGATAT‑CCTGCTAAGATGCGTCAGCGACGAATCATGCGTTACACACCTGATGCGAAAAATTATGGACGAACTCCGTCATACACTTCAGTCCAGCGCAGCGCGTCTTTAACCGCTTGCAGGAGGGTG‑GTGTTATACAT‑CACCGTggtgtctatgcagtgacatc > 2:73815/2‑232 (MQ=9) gTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGc > 1:280445/1‑251 (MQ=25) gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGgc < 1:485226/251‑1 (MQ=25) gcgcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTGCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCg < 1:203741/251‑1 (MQ=25) cGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGA‑TCGTTATCAAT‑CAACCTGATTTTCATGTCGTA‑CATCTCGGCGacccggtgcatacacttgtggttc > 2:417907/1‑227 (MQ=21) gaaTCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGGCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACGTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTAGCAAT‑CACCGGGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAg < 2:295393/249‑1 (MQ=25) cATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAgc < 1:512529/251‑2 (MQ=37) aGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCCTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTTGGGTTCAgcgcct > 1:173148/1‑246 (MQ=37) ggCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGGGATTTCAATGTCGGG‑CATCTCGGCGAACAGGCGCATATCGTTGAGGGTCAGCGCATg > 1:637094/1‑251 (MQ=25) ggCGTTGAATGCCGGATGCGCTTTGCTTAGCCGGCCTACAAAATCGCAGCTTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATg < 1:699048/251‑1 (MQ=255) ttGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCG‑GAGATCGTTATCAAT‑CACCGTGATTTCAATGTCGGG‑AATCTTAGCGAACCGGCGCataacggttaggatcagcgccaggctg > 1:380731/1‑227 (MQ=21) cGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGGTCCGCGCATCGC‑T‑GAAGACggg > 1:428156/1‑250 (MQ=37) ggATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGGACCGGCGCATATCGTTGGGGGTCCGCGCATGGC‑T‑GAAGACggg > 1:71588/1‑249 (MQ=25) gcTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTgc < 1:31632/251‑1 (MQ=38) cTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGA‑TCGTTATCAAT‑CACCGTGATTTCCATGTCGTG‑CATTTCGGCGAAATGGCGCATATCGTTGAGGGTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGccc > 1:234604/1‑250 (MQ=37) ttATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGaactg < 1:80130/198‑4 (MQ=37) aTCCGGCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGCCGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGGTTTTAAT‑CAACGTGTTTTCAATTTC‑TGTCATCTGGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCCTGGC‑TTGCAG‑CGGTCTGGTGCGCGccaccc > 2:298902/1‑250 (MQ=19) cGGCCTACAAAATCGCAACGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGA‑TCGTTATTAATACACGGT‑ATTTCAATGTCGTG‑CATCTTGGCGTACTCGCGCATATCGGTGGGGGTCAGCGCATGGC‑T‑GGAGACGGGATTGGGCGGGCCACCcgcg > 2:347379/1‑251 (MQ=255) gCCTACAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACAAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGAG‑TCGGTATCAAA‑TAACGAGATTTCATTATCGTT‑CATTTCGGCGAACTGGCCCCTATAGGTGAGGGTTACGGCATGGC‑T‑GAAGACgggagaggtggtgccacccgca > 1:370894/1‑229 (MQ=255) aCAAAATCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTAGTTCCAGCGCAGCGCGTCTTTAAACGCTGGAAGGCGGGTA‑TCGTTATCCCA‑CACCGTGTTTTCCATATAGTG‑CATATCGGCGAATTGGCGCATATCGTTTGGGGTCAGCGCCTGTCAT‑GA‑GACGGTATGGGGGGGGCCACCCGCGAGGATc > 2:534223/1‑251 (MQ=255) aaagcGCAGCGTG‑TAGGCCAGATAAGACGCGGCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTG‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATTGTGCGCGCCACCAGCGAGGATCCAc < 2:301142/247‑1 (MQ=255) aaaacGCAGCGTG‑TAGGCCTGATAAGACGCGTAAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCAc < 2:328889/247‑1 (MQ=255) aaaTCGCAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCAc > 1:484964/1‑251 (MQ=255) cAGCGTG‑TAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCg < 2:355268/251‑1 (MQ=255) aGCGTG‑TAGGCCTGACAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCCTGGC‑T‑GAAGACGGTATGGGGCGCGCCACCAGCGAGGAACCACGCTTCgg > 1:432032/1‑251 (MQ=255) gtg‑tAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GGAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGGTTCGGa > 1:478823/1‑249 (MQ=255) gtg‑tAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGAATGGTGCGCGCCACCAGCCAGGATCCCCGCTTCGGa > 1:66449/1‑249 (MQ=255) aGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGCTTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCCTATCGTTGTGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGGGCGCCCCCAGCGGGGAGCCCCGCTTCGGAAGCAgg > 2:381247/1‑250 (MQ=255) ggccttaTAAGACGCGGCAGCGTCGCATCAGTCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGGCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTGCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGtt < 2:238413/245‑1 (MQ=255) ggCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAgcg < 1:586858/192‑3 (MQ=255) tgATAAAACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGAA‑TCGTTATCAAT‑CACCGTGGTTTCAATGCAGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCCAGGA‑T‑TAAGACGGTAAGTTTCGCGCCCCCATCGAGGGTCCACGCTTCGGGAGCAGTTGGc > 1:127941/2‑250 (MQ=255) tAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGGACTGGCGCATAACGTTGAGGGTCAGCGCATGGC‑T‑GAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGGAGCAGTTGGCAGa > 1:296606/1‑250 (MQ=255) aaGACGCGTCAGCGTTGTATCAGGCGTTACACCCCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTGCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTGCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAt < 2:663465/250‑1 (MQ=255) aaGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAt < 1:381247/250‑1 (MQ=255) aGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATcc < 1:671835/251‑1 (MQ=255) aGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATcc < 2:194814/251‑1 (MQ=255) aCGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCgg < 1:264377/251‑1 (MQ=255) cAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTgcg < 1:290081/250‑1 (MQ=255) cGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGGG‑CATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAACGCATGGC‑T‑GAAGACGGGATGGGGCGCGCCACCAGGGCAGATCCACGCCTTGGAAGcgggttggagatacgggttcgcttt > 2:454739/1‑227 (MQ=255) gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTc < 1:181078/251‑1 (MQ=255) cacctgATGCGACTACTTAGCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTT‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCatc < 2:285378/246‑3 (MQ=255) acctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGCG‑CATCTCGGCGGACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GGAGGCCGTATTGGGCCCGCCACCAGAGAGGATCCACGCCTCCGGAGCCGTTGGGAGGTCCGGGTTG‑GCTTTccccaccgccatcgcccccc > 2:625289/5‑233 (MQ=255) cctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCgg > 1:318767/4‑251 (MQ=255) cctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCgg < 1:347379/248‑1 (MQ=255) gATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTA‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCa < 2:637094/250‑1 (MQ=255) | CCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCC‑TGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTG‑TAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTG‑TCGTTATCAAT‑CACCGTGATTTCAATGTCGTG‑CATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGC‑T‑GAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGG‑TTGCGCTTTCCACAGCGCATTCGCCACCGGCA > NC_000913/66585‑67073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |