Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,641	T→A	R380R (CGA→CGT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,641	0	T	A	93.0%	178.7 / ‑0.3	58	R380R (CGA→CGT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (22/31);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

AGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCC  >  NC_000913/64403‑64871                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         agaACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATag                                                                                                                                                                                                                              <  1:685354/251‑1 (MQ=255)           aTCGAGAAAACCTTCGGTACTGTGCCCTGGATCAGGCCGCGCCTTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGTCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGACAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaa                                                                                                                                                                                                                    <  2:388378/251‑1 (MQ=255)             cGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGAAGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCTCGTAGCCGCCAGGGCTGGCGTGTGGCTGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaaac                                                                                                                                                                                                                  <  1:324449/251‑2 (MQ=255)              gagaAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaaacg                                                                                                                                                                                                                 >  1:144743/1‑249 (MQ=255)                      tttCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCCCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAACCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAc                                                                                                                                                                                                          <  2:557999/249‑1 (MQ=255)                       ttCGGTACTGTGCTCTGGATCAGGCTGCGCCCTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAc                                                                                                                                                                                                          >  1:557999/1‑249 (MQ=255)                            tACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGAACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGACTCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGAGCGACATAACCAGCGCGGGGCATACGGGGAAAATAGAAGTGACCAACCGCCGCCACCGatccc                                                                                                                                                                                                   >  2:339844/1‑247 (MQ=255)                                gtgtgcgggATAAGGCTGCGCAAGGCTTTCCCCCAGACCGACGGGATCAACCAGAAAGGTGCGGATGAGAGACGGGTACAGGCTTTTATAGTCCAGCACCAGCCCT‑GAATCATAAAGCCC‑TGGCCGTGAATCAATCACGTAGCCGCCAGGGCGGGCGTGCGGCGGCACTGCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCGCCGAAccgcc                                                                                                                                                                                                 <  2:53577/242‑1 (MQ=255)                                gtgCTCTGGATCAGTCGGGGCCCTGCCTTCCCCCAGCCCGACGTGATCAATCCGAGATGTGCGGAGTATCGACGGTTCCAGGCTTTTATAGTCCAGCACCCTTACC‑TAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACAGAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCTGCCACCGatcccccg                                                                                                                                                                                                <  2:430945/250‑8 (MQ=255)                                        gATCAGGCTGCGCCATGCCTTCCTCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTct                                                                                                                                                                                       <  1:476642/251‑2 (MQ=255)                                            aGGCTGCGCCATGCCTGCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGAGGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCg                                                                                                                                                                                   <  2:252097/251‑1 (MQ=255)                                              gtcgcgcCTTGCCGTCCACCAGCCCGACGGGATCAATCAGAAGGTTTCGGATGATCGACGGGGACTGGCTTTTATAGTCCAGCACCAGTACC‑GAATCAAAAAGCCC‑TGTCCGTGAATCCATCACGTAGCCGCCAGTGCTGTCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACTAGGAAAATAGATATGACCAAACGCCGCCACCGatcccctgtggcggtcttccggc                                                                                                                                                                                 <  2:248971/248‑23 (MQ=255)                                               cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTAcc                                                                                                                                                                                    <  1:97250/247‑1 (MQ=255)                                               cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCAAccagtg                                                                                                                                                                                >  2:97250/1‑247 (MQ=255)                                                tGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGc                                                                                                                                                                                 >  1:171947/1‑249 (MQ=255)                                                             ccaccgGCCCGGCGGGATCAATCAGAAAGGGGCGGAGGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAAGCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGACGTTCACCGtc                                                                                                                                                                  <  2:317089/251‑2 (MQ=255)                                                              caccaGCCTGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcg                                                                                                                                                                 <  2:240962/251‑3 (MQ=255)                                                               cccaGCCCGACGTGATGAATCAGAAAGGTGCGGATGACCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGTATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcgc                                                                                                                                                                <  1:321135/250‑4 (MQ=255)                                                                    cccaagggtatcaaacaAAAAGGGGCGGAGGATCGAGGGGTACAGGCTTTTAGAGCCCAGCAC‑AGGACTTAAATCAAAAGGCCC‑GGCCCGCGAATACATCAGTTAGCCCCCAGGGCGGGCGGGCGGGGCCACTTCGCGGAGATTAGGCGCGACAAACCCAGCGCGATGTATTCGCGAAAAATAGAGTGGACCAAATGCCGCCCCCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGtt                                                                                                                                                           <  2:238911/242‑1 (MQ=255)                                                                        aCGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGatcccccg                                                                                                                                                                                                <  1:454067/210‑8 (MQ=255)                                                                          gggATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGt                                                                                                                                                      >  1:271167/1‑250 (MQ=255)                                                                             atcaatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCAAGGAGCCGCCAGGGCTGGCGTGCGGCGGCCATTCGCCGAGATTCGGCGCGTCATAAACAGCGCGATGCATTCGCGGACAATAGAGATGATCAAGAGCCGCCAACGAACCACCGTGTCGGTACACCGGCAGGCCGTTCACCGTTGcccctccaaggcaa                                                                                                                                                   >  2:157314/1‑239 (MQ=255)                                                                                  tcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCAcccgccctcgttcggtgtacaatggc                                                                                                                                             >  2:630101/1‑227 (MQ=255)                                                                                      aaaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGACCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGa                                                                                                                                         <  1:229414/251‑1 (MQ=255)                                                                                       aaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGAt                                                                                                                                        >  1:124074/1‑251 (MQ=255)                                                                                            gCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCa                                                                                                                                    <  1:298861/250‑1 (MQ=255)                                                                                               gatgagagACGGGTACAGGCTTTGATAGTCCAGCACCAGTACA‑GAATCATAAAGCCCGTGGC‑GTGAATCCATCACGGAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTATGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAACGATCCCCCGTGGAGGTCTACCGGCAGGCCGTTCACCGTCGCGCGTTCGAGTAAAAATGGCATGATTTCAGttt                                                                                                                                <  2:104982/244‑1 (MQ=255)                                                                                                                ggCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTc                                                                                                               >  1:470418/1‑251 (MQ=255)                                                                                                                  cTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGCTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCCGTTTTATgggaaaaccgcgtcac                                                                                                             >  2:190035/1‑237 (MQ=255)                                                                                                                   ttttATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCAcc                                                                                                            >  1:363444/1‑251 (MQ=255)                                                                                                                        taGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATCCGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGATCACCGGCGCTCGTTCGGGTAAAAAAGGCCTTATTTCCGTTTTATTGAAAATCTGCGTCACCaacac                                                                                                       >  2:555262/1‑247 (MQ=255)                                                                                                                          agtccggctccaggtccgAATAATAAAGCCC‑GGGCCGTGAATCCATCACGTAGCCGCCAGGGCTTGCGGGTGGGGGCACTACGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAACCTGCGTCACCAGCTCg                                                                                                      <  2:278578/234‑1 (MQ=255)                                                                                                                                         ccgAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                                                       <  2:144743/249‑1 (MQ=255)                                                                                                                                           gAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCCCCCGGTCGGGTAAAAATGGCCTGGTTTCAGTTTTTTGGAAAATCTTCGTCACCAACTCGCAATCTTTTCGGTtat                                                                                      >  2:162730/1‑250 (MQ=255)                                                                                                                                           aaaTCATAAAGCCC‑TGGCCGGTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACGTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCTTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGATAATCTGCGTCACCAGCTCGCAATCTTTCAGGTtata                                                                                     <  2:330047/250‑1 (MQ=255)                                                                                                                                                 tAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCATCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGtcgc                                                                               >  1:563692/1‑248 (MQ=255)                                                                                                                                                              cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGttt                                                                   >  1:114403/1‑251 (MQ=255)                                                                                                                                                                 gAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATc                                                                <  1:502053/251‑1 (MQ=255)                                                                                                                                                                    cccATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTc                                                             <  2:271167/250‑1 (MQ=255)                                                                                                                                                                     ccaacACGTAGGCGCCAGGGCGGGCGTGTGGCGGCTCTTCGCCGTGATTAGTCGCGACATACACAGCGCGGGGCCTACGAGGAAAATAGAGATGACCAAACGCCTCCACCGATCCCCCGTGGCGGTCGAGCGGCAGGCCTTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGTTTATCTTCg                                                            <  2:547186/247‑1 (MQ=255)                                                                                                                                                                       aTCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑Gc                                                         >  1:425597/1‑251 (MQ=255)                                                                                                                                                                         cACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGa                                                       <  1:4777/251‑1 (MQ=255)                                                                                                                                                                         cACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCg                                                        >  1:211800/1‑250 (MQ=255)                                                                                                                                                                                    cAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAGTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCa                                            <  2:425597/251‑1 (MQ=255)                                                                                                                                                                                     aGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCaa                                           <  1:617974/251‑1 (MQ=255)                                                                                                                                                                                       ggcgggcgagtggcggcACTTCGCCGAGATTAGGCTCGACATAACCAGAGCGGGGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCTATACTCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCGCGCAATCTTTTAGGTTATAAGTCGCTAGCGCAGGTTTATCTTCG‑GCGAAACGTCGGTCAA‑tt                                        <  2:253924/240‑1 (MQ=255)                                                                                                                                                                                       ggCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑tt                                        >  1:137699/1‑251 (MQ=255)                                                                                                                                                                                            gCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGGTTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTAGCCAGCGCAGGTTTATCTTCGAGC‑AAACGGCGGGCAA‑ttttttc                                   >  2:170983/1‑247 (MQ=255)                                                                                                                                                                                                    cggcACTTCGCCGAGATTAGGCGCGACTTAACCAGCGCGGTGCATACGAGGAAAATAGAGATGGCCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGAt                           <  2:470418/251‑1 (MQ=255)                                                                                                                                                                                                              ccGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCCTTCGATCCCACggggt                 >  1:212743/1‑249 (MQ=255)                                                                                                                                                                                                               cGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGt                                                                                                                <  1:567056/157‑1 (MQ=255)                                                                                                                                                                                                                   aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTATCgt            >  1:364266/1‑250 (MQ=255)                                                                                                                                                                                                                   aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTATCGa            <  1:339844/251‑1 (MQ=255)                                                                                                                                                                                                                    ttAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTAACGAt           >  1:650974/1‑251 (MQ=255)                                                                                                                                                                                                                          gcgACAGAACCAGCGCTGTGCATACGCGGAAAATAGAGATGACCAAACGCCGCCTCCGATCCCCCGTGGCGGGCTACCGGCAGGCCGTTCACCGTCGATCGTTCGAGTAAAAATGGCTGGAGTTCAGTTTTATGGAAAAGCTGCGTCCCCAGCGCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑GTGCGTCCATTCGATCCCGCGGGTTATCGATAGAttt     <  2:619079/251‑1 (MQ=255)                                                                                                                                                                                                                           cgACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGGCAAATTTCG‑CCCTTCGTTCCCCCGGGGTATCGAGAGAtttt    >  2:13574/1‑251 (MQ=255)                                                                                                                                                                                                                            gACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTATCGATAGAttttt   >  1:118258/1‑250 (MQ=255)                                                                                                                                                                                                                             aCATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTcc  <  1:512754/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         AGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCC  >  NC_000913/64403‑64871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑