Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,3470TA96.9% 105.0 / ‑3.3 32intergenic (+138/‑131)metA/aceBhomoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (13/18);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCT  >  NC_000913/4215109‑4215584
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
ggCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATg                                                                                                                                                                                                                                    <  2:163597/250‑1 (MQ=255)
              caTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAAATCACGCCCTACGATCTACGGCACATGAATCCACCGCTGGATTAATCTTATGTGATAGTCGCTCGTTAAGCGATTCAGCACCTTAACTCAGGCACCTTCGGGTGCATTTTTTTTTTCCGAAACGTCCCTCAGCAGGTGAATAAATTTTATTCATATTTTTATCAACAAGTTATCAAGTATTTATAATTACAATGGAAATTGTTTTTgtt                                                                                                                                                                                                                     >  2:289475/2‑249 (MQ=255)
                      gTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCAt                                                                                                                                                                                                              <  1:277744/250‑1 (MQ=255)
                       tttACCAACTGGCTCACCTATTACGTCTTCCAGATCACGTCATACGAACTACGGCACATGAATTCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATGCAGCACCTTACCTCAGGCACCTTCGTGTGCCTTTTTTATTTCCGAAACGTACCGCAGCAGGTTAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATGGTTTTTGATTTTGCAtt                                                                                                                                                                                                             <  2:603568/250‑1 (MQ=255)
                                  gCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTCCCTCAGCAGGGGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTCCATTTTAATTGagt                                                                                                                                                                                                   >  1:430766/1‑249 (MQ=255)
                                                            cGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGc                                                                                                                                                                       <  1:172686/251‑1 (MQ=255)
                                                                  actaacTACGGCACATGAATCCAACGGTGGATTAATCCTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCGTACCTCAGGCTCCTTCGGGTGCTTTTTTTATTTCCGAAACGTAACGCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAAGTGTTTTTGATTTTGCATTGTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAATAGCACAACg                                                                                                                                                                 <  2:104916/246‑1 (MQ=255)
                                                                         aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATcc                                                                                                                                                             <  1:458925/248‑1 (MQ=255)
                                                                            gCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGtt                                                                                                                                                       >  2:191296/1‑251 (MQ=255)
                                                                              acaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTca                                                                                                                                                     <  1:212432/251‑1 (MQ=255)
                                                                              acaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTc                                                                                                                                                      >  1:391069/1‑250 (MQ=255)
                                                                                aTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTcaca                                                                                                                                                   <  1:307904/251‑1 (MQ=255)
                                                                                    aTCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTgg                                                                                                                                               <  1:172856/251‑1 (MQ=255)
                                                                                                   aaTCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATc                                                                                                                                >  1:680200/1‑251 (MQ=255)
                                                                                                           gtgATAGTCGAGCGTTAAGCGATTCAGCAACTTACCTCAGGCTCCTTCGGGTGCCTTTTTTAGTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAAGTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACg                                                                                                                         <  2:531679/250‑1 (MQ=255)
                                                                                                            ggATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCGCCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATGTTATTCATATTGTTATCAATAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACgtg                                                                                                                       <  2:701629/250‑3 (MQ=255)
                                                                                                                   cGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGc                                                                                                                >  1:408227/1‑251 (MQ=255)
                                                                                                                            aGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACt                                                                                                       <  1:149024/251‑1 (MQ=255)
                                                                                                                               gATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGaa                                                                                                    <  2:621135/251‑1 (MQ=255)
                                                                                                                                   cAGCACCTTACCTCAGGCACCTTCTGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAg                                                                                                 <  1:634477/250‑1 (MQ=255)
                                                                                                                                                     aCCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAAc                                                                              <  1:102417/251‑1 (MQ=255)
                                                                                                                                                                               aaaCGGACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTAGTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTGCGGATCCATGACGAGGAGCTGCACGAAGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAg                                                    <  2:671253/251‑1 (MQ=255)
                                                                                                                                                                                    tACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAGCAAGTTATAAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGAAGTATTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGCTGACTGAACAGGCAACAACACCCGATGAACTGGCTTTCACATGGCCGTCAGGCGAGCAga                                                >  2:202223/1‑249 (MQ=255)
                                                                                                                                                                                         cagcagGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGc                                          >  1:190271/1‑251 (MQ=255)
                                                                                                                                                                                            cagGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCaaa                                       <  1:354718/251‑1 (MQ=255)
                                                                                                                                                                                                tGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCt                                   >  1:145615/1‑251 (MQ=255)
                                                                                                                                                                                                 gAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCGAATTCtt                                  <  1:191296/251‑1 (MQ=255)
                                                                                                                                                                                                      aaTTTTATATATAATGGTATCAACATGTTATCAAGTATTGATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGAGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACATTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGATTGAACAGTCAACAACTACAGATGAACTGGCTTTCACCAGGCCGTATGGTTAGCAGGATAAGCAAATTCTTACTGc                             <  2:47128/251‑1 (MQ=255)
                                                                                                                                                                                                                        aTCAACAAGTTATCAAGTATTTATAATTAAACTGGAAATTGTTTTTGATTTTGCATTTTAAATGAGAAGTCTTAGTTGTTCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATAAACTGGCTTTCACAAGGACGTATGGCGAGCAGAAGAAGCAAATTCTTACTGCCGAAGCGGTAGAAtttat           >  1:521501/1‑249 (MQ=255)
                                                                                                                                                                                                                        aTCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTct           >  1:440760/1‑251 (MQ=255)
                                                                                                                                                                                                                         tCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTctg          >  1:87467/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ttATCAAGTATTTATAATTAACATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGTGCAGGAGAAGCAAATTCTTACTGACGAAGCGGTAGAATTTCTGACTGAGCt  >  2:507722/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
GGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCT  >  NC_000913/4215109‑4215584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑