Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,593	C→T	P396P (CCG→CCA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,593	0	C	T	93.0%	158.8 / ‑1.2	58	P396P (CCG→CCA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (22/31);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAATTTCGTC  >  NC_000913/64351‑64838                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cgcCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCAc                                                                                                                                                                                                                                                >  1:330005/1‑251 (MQ=255)             aTGTTAGTCACAATCCCCGGCAGGCCAGGTTTTTCTCGCGAGACCCAGTCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCTTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAtt                                                                                                                                                                                                                                    <  2:543654/251‑1 (MQ=255)                   gTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGgcgc                                                                                                                                                                                                                              <  1:608797/251‑1 (MQ=255)                       caATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAAACTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGAcc                                                                                                                                                                                                                          >  1:59828/1‑250 (MQ=255)                             ccGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATaa                                                                                                                                                                                                                       <  1:409718/248‑1 (MQ=255)                              cggccggcAATGTTTTTCTCGCGAGAACCAGGAATCGAGAAAACCTTCGGCACTAAGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCCGAAAGGGGCGGATGATAGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑GGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGAGGGGCGGCACTTCGCCGGGATTAGGCGGGACATAAcc                                                                                                                                                                                                                     >  1:633532/1‑249 (MQ=255)                              cGGCAGGCAATGTTTTTCTCGCTAGAACCAGGCATCGAGAAAACCTTCGGTACTTTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAg                                                                                                                                                                                                                   <  1:562324/251‑1 (MQ=255)                                gcaggcaATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCCCCGGGATTAGGCGCGACATACCCAgcg                                                                                                                                                                                                                 >  2:502053/1‑251 (MQ=255)                                       aTGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACAGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCa                                                                                                                                                                                                          <  1:605497/251‑1 (MQ=255)                                                     agaACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATag                                                                                                                                                                                            <  1:685354/251‑1 (MQ=255)                                                         ccAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCAtacgagg                                                                                                                                                                                                   <  1:189219/240‑7 (MQ=255)                                                         ccAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCAtacgagg                                                                                                                                                                                                   >  2:189219/1‑234 (MQ=255)                                                               aTCGAGAAAACCTTCGGTACTGTGCCCTGGATCAGGCCGCGCCTTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGTCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGACAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaa                                                                                                                                                                                  <  2:388378/251‑1 (MQ=255)                                                                 cGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGAAGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCTCGTAGCCGCCAGGGCTGGCGTGTGGCTGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaaac                                                                                                                                                                                <  1:324449/251‑2 (MQ=255)                                                                  gagaAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaaacg                                                                                                                                                                               >  1:144743/1‑249 (MQ=255)                                                                          tttCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCCCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAACCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAc                                                                                                                                                                        <  2:557999/249‑1 (MQ=255)                                                                           ttCGGTACTGTGCTCTGGATCAGGCTGCGCCCTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAc                                                                                                                                                                        >  1:557999/1‑249 (MQ=255)                                                                                tACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGAACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGACTCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGAGCGACATAACCAGCGCGGGGCATACGGGGAAAATAGAAGTGACCAACCGCCGCCACCGatccc                                                                                                                                                                 >  2:339844/1‑247 (MQ=255)                                                                                    gtgtgcgggATAAGGCTGCGCAAGGCTTTCCCCCAGACCGACGGGATCAACCAGAAAGGTGCGGATGAGAGACGGGTACAGGCTTTTATAGTCCAGCACCAGCCCT‑GAATCATAAAGCCC‑TGGCCGTGAATCAATCACGTAGCCGCCAGGGCGGGCGTGCGGCGGCACTGCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCGCCGAAccgcc                                                                                                                                                               <  2:53577/242‑1 (MQ=255)                                                                                    gtgCTCTGGATCAGTCGGGGCCCTGCCTTCCCCCAGCCCGACGTGATCAATCCGAGATGTGCGGAGTATCGACGGTTCCAGGCTTTTATAGTCCAGCACCCTTACC‑TAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACAGAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCTGCCACCGatcccccg                                                                                                                                                              <  2:430945/250‑8 (MQ=255)                                                                                            gATCAGGCTGCGCCATGCCTTCCTCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTct                                                                                                                                                     <  1:476642/251‑2 (MQ=255)                                                                                                aGGCTGCGCCATGCCTGCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGAGGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCg                                                                                                                                                 <  2:252097/251‑1 (MQ=255)                                                                                                  gtcgcgcCTTGCCGTCCACCAGCCCGACGGGATCAATCAGAAGGTTTCGGATGATCGACGGGGACTGGCTTTTATAGTCCAGCACCAGTACC‑GAATCAAAAAGCCC‑TGTCCGTGAATCCATCACGTAGCCGCCAGTGCTGTCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACTAGGAAAATAGATATGACCAAACGCCGCCACCGatcccctgtggcggtcttccggc                                                                                                                                               <  2:248971/248‑23 (MQ=255)                                                                                                   cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTAcc                                                                                                                                                  <  1:97250/247‑1 (MQ=255)                                                                                                   cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCAAccagtg                                                                                                                                              >  2:97250/1‑247 (MQ=255)                                                                                                    tGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGc                                                                                                                                               >  1:171947/1‑249 (MQ=255)                                                                                                                 ccaccgGCCCGGCGGGATCAATCAGAAAGGGGCGGAGGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAAGCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGACGTTCACCGtc                                                                                                                                <  2:317089/251‑2 (MQ=255)                                                                                                                  caccaGCCTGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcg                                                                                                                               <  2:240962/251‑3 (MQ=255)                                                                                                                   cccaGCCCGACGTGATGAATCAGAAAGGTGCGGATGACCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGTATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcgc                                                                                                                              <  1:321135/250‑4 (MQ=255)                                                                                                                        cccaagggtatcaaacaAAAAGGGGCGGAGGATCGAGGGGTACAGGCTTTTAGAGCCCAGCAC‑AGGACTTAAATCAAAAGGCCC‑GGCCCGCGAATACATCAGTTAGCCCCCAGGGCGGGCGGGCGGGGCCACTTCGCGGAGATTAGGCGCGACAAACCCAGCGCGATGTATTCGCGAAAAATAGAGTGGACCAAATGCCGCCCCCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGtt                                                                                                                         <  2:238911/242‑1 (MQ=255)                                                                                                                            aCGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGatcccccg                                                                                                                                                              <  1:454067/210‑8 (MQ=255)                                                                                                                              gggATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGt                                                                                                                    >  1:271167/1‑250 (MQ=255)                                                                                                                                 atcaatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCAAGGAGCCGCCAGGGCTGGCGTGCGGCGGCCATTCGCCGAGATTCGGCGCGTCATAAACAGCGCGATGCATTCGCGGACAATAGAGATGATCAAGAGCCGCCAACGAACCACCGTGTCGGTACACCGGCAGGCCGTTCACCGTTGcccctccaaggcaa                                                                                                                 >  2:157314/1‑239 (MQ=255)                                                                                                                                      tcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCAcccgccctcgttcggtgtacaatggc                                                                                                           >  2:630101/1‑227 (MQ=255)                                                                                                                                          aaaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGACCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGa                                                                                                       <  1:229414/251‑1 (MQ=255)                                                                                                                                           aaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGAt                                                                                                      >  1:124074/1‑251 (MQ=255)                                                                                                                                                gCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCa                                                                                                  <  1:298861/250‑1 (MQ=255)                                                                                                                                                   gatgagagACGGGTACAGGCTTTGATAGTCCAGCACCAGTACA‑GAATCATAAAGCCCGTGGC‑GTGAATCCATCACGGAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTATGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAACGATCCCCCGTGGAGGTCTACCGGCAGGCCGTTCACCGTCGCGCGTTCGAGTAAAAATGGCATGATTTCAGttt                                                                                              <  2:104982/244‑1 (MQ=255)                                                                                                                                                                    ggCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTc                                                                             >  1:470418/1‑251 (MQ=255)                                                                                                                                                                      cTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGCTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCCGTTTTATgggaaaaccgcgtcac                                                                           >  2:190035/1‑237 (MQ=255)                                                                                                                                                                       ttttATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCAcc                                                                          >  1:363444/1‑251 (MQ=255)                                                                                                                                                                            taGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATCCGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGATCACCGGCGCTCGTTCGGGTAAAAAAGGCCTTATTTCCGTTTTATTGAAAATCTGCGTCACCaacac                                                                     >  2:555262/1‑247 (MQ=255)                                                                                                                                                                              agtccggctccaggtccgAATAATAAAGCCC‑GGGCCGTGAATCCATCACGTAGCCGCCAGGGCTTGCGGGTGGGGGCACTACGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAACCTGCGTCACCAGCTCg                                                                    <  2:278578/234‑1 (MQ=255)                                                                                                                                                                                             ccgAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                     <  2:144743/249‑1 (MQ=255)                                                                                                                                                                                               gAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCCCCCGGTCGGGTAAAAATGGCCTGGTTTCAGTTTTTTGGAAAATCTTCGTCACCAACTCGCAATCTTTTCGGTtat                                                    >  2:162730/1‑250 (MQ=255)                                                                                                                                                                                               aaaTCATAAAGCCC‑TGGCCGGTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACGTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCTTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGATAATCTGCGTCACCAGCTCGCAATCTTTCAGGTtata                                                   <  2:330047/250‑1 (MQ=255)                                                                                                                                                                                                     tAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCATCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGtcgc                                             >  1:563692/1‑248 (MQ=255)                                                                                                                                                                                                                  cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGttt                                 >  1:114403/1‑251 (MQ=255)                                                                                                                                                                                                                     gAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATc                              <  1:502053/251‑1 (MQ=255)                                                                                                                                                                                                                        cccATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTc                           <  2:271167/250‑1 (MQ=255)                                                                                                                                                                                                                         ccaacACGTAGGCGCCAGGGCGGGCGTGTGGCGGCTCTTCGCCGTGATTAGTCGCGACATACACAGCGCGGGGCCTACGAGGAAAATAGAGATGACCAAACGCCTCCACCGATCCCCCGTGGCGGTCGAGCGGCAGGCCTTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGTTTATCTTCg                          <  2:547186/247‑1 (MQ=255)                                                                                                                                                                                                                           aTCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑Gc                       >  1:425597/1‑251 (MQ=255)                                                                                                                                                                                                                             cACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGa                     <  1:4777/251‑1 (MQ=255)                                                                                                                                                                                                                             cACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCg                      >  1:211800/1‑250 (MQ=255)                                                                                                                                                                                                                                        cAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAGTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCa          <  2:425597/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCaa         <  1:617974/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAAtt       >  1:137699/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGGTTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTAGCCAGCGCAGGTTTATCTTCGAGC‑AAACGGCGGGCAAttttttc  >  2:170983/1‑247 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAATTTCGTC  >  NC_000913/64351‑64838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑