| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,869,637 | C→A | L87L (CTC→CTA) | cbrA → | colicin M resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,869,637 | 0 | C | A | 90.0% | 183.7 / 2.7 | 70 | L87L (CTC→CTA) | cbrA | colicin M resistance protein |
| Reads supporting (aligned to +/- strand): ref base C (4/3); new base A (39/24); total (43/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGAT > NC_000913/3869396‑3869880 | cgagtattGGCCGCGGCCCGTCAGGATCGGCGTTGGCACGAAAGGTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGGGGTACTGATGGTGTCAGCACACCCTGCGGCGGTCTGCTGGCACCTGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑AGCGCTAACCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAAc < 2:420606/247‑1 (MQ=255)cGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGTACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAAc < 1:483945/251‑1 (MQ=255) gATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCCCCGGACGCGCCGCGGTCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGGG‑ATCGCTAATCCGCAGATTTTTAGCGTCAAAACCGTCGGCGTCGCCGCCTCGCTCACGCGTAACt > 2:5554/1‑251 (MQ=255) attagCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAAACCTGCGGCGGTCTGCTGGCACCGGACGAGCAACGGTCTTTTATTCGCGGTGGGCTGACGCTTCCTGTC‑GAGGGG‑ATCGCTAAACCGCAGAGTTTCAGCGTCAAAACCGTCGACGACGCAGCATCGCGAACGCGTAACTAcc > 2:517929/5‑250 (MQ=255) tGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGc > 1:603838/1‑251 (MQ=255) cTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCCGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTTCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGGCGCCGCATAGCTAACGCGTAACTACCAGCGa > 1:612418/1‑249 (MQ=255) ccGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGc < 1:498263/250‑1 (MQ=255) gCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCaa < 1:5554/251‑1 (MQ=255) aGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAata < 1:150311/251‑1 (MQ=255) ccGGCGTTGGCACCAAAGTTAGCAGGCAAAATGCAGGTTATCGCGCTGGATAAAAAGCACCAGTGTGGTACTTAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGATGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTaa < 2:682983/250‑1 (MQ=255) ggCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATc < 1:546000/251‑1 (MQ=255) cGTTGGCACGCAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGAGACGCGCTCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑CTCGCTTCTCAGCAGCTTTTCAGCGTCAAAACCGTCGCCGACGCCGCCTAGATAAAGCGTAACTACACGCGAAGCTATATCAATA‑TTAATcac > 2:209320/1‑249 (MQ=255) gTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGCAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGcc > 1:680719/1‑251 (MQ=255) gggCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAATGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCa < 2:224114/249‑1 (MQ=255) gCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGCTAAAAAGCACCAGTGTCCTACTGAAGGTTTCCGCAAACCCTGCGGAGGTCTGCTGGCCCCGGACGCGCCGCGCTCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTTAGCGTCCAAACCGCCGACGTCGCCGCATCGGTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATgg > 1:336347/1‑250 (MQ=255) gAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTc < 1:115044/251‑1 (MQ=255) gAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTAGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGACATGCtttt > 2:527954/1‑250 (MQ=255) aacgTTAGCAGGCAAAATGCAGGCGATCGCGCGGGATAACAAGCACCAGTGAGGTATTGAAGGTTTCAGCAAACCCTGCGGCGATCTGCTGGCACCGGACTCGCAGCGATCTTTTATTCGCGATGGGCTCACGCGTCCTGTC‑GATGTG‑ATCGCTCATCCGCAGATTTTCAGCGTCAAAACCGTCGACACCGCCGAATCGCTCACGCGACACTACCAGCAACGCTCTCTCACTA‑TTAATTGCAATGCTTTCg > 2:487313/4‑251 (MQ=255) gTTAGCCGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTAAGCAAACCCTGCGGCGGTCTGCTGGCACCGGAAGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAACACCGTCGACGTCCCCGCATCGCTAACGCGGAACTAACAGCGACGCTATATCAATA‑TTAATCGCCATGCTTTCGACt > 2:302389/1‑251 (MQ=255) cAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATg > 1:451335/1‑250 (MQ=255) cAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTACTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTTCCAGCGCAGCTATATCAATA‑TTACACGCACTGCTTTCGACTTGGGGATgt > 2:70273/1‑250 (MQ=255) aacaTGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGAACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCCCCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGATAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCCTCGCTCACGCGGAAATACCAGCGCAGCTATATCAATA‑TTACTCGCCCTGCTTTCAGCTTGTGGATgc > 2:405337/4‑249 (MQ=255) ggATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATc < 2:182225/250‑1 (MQ=255) aaaaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTg > 1:170726/1‑249 (MQ=255) aaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGt > 1:571591/1‑251 (MQ=255) gCACCAGAGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGAGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGAGTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCCATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCACTGATCCCCGCCAGCGTTGAGGttt > 1:484993/1‑251 (MQ=255) cacccaggtgggTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCTGCGATCTTTTATTCGCGAGGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCATATTTCCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAAGA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTAGAGGttg < 2:447226/241‑2 (MQ=255) aCCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTAt > 1:378440/1‑251 (MQ=255) gtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGAc > 1:170489/1‑251 (MQ=255) aaGGCTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGCTCCTTTATTCGCGATGGGCTGACGCTTCCTGCC‑CATGTG‑ATCGCTCATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGAATCGCTCCCCCGTCAATACCAGCGAAGCTATAACAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGCTTATCACGACAGCCTGTGc > 1:36179/1‑251 (MQ=255) agcaaccccggggggggTCTGCTGGCCCCGGACGCGATGCGTTCTTTTATTCCCGATGGGCTGCCGCTTCC‑GTCCGATGTG‑ATCCCTAATCCGCAGATTTTCAGCGTCAAAACCGTAGACGTCGCCGCATCGCTACCGCGTAACTCCCAGCGAAGTTATATCAATA‑TTAATCGCATTGCTTTCGACTTGTGGATGAAATCGCCGATCACCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAAt < 2:150353/236‑1 (MQ=255) aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCg < 1:697246/208‑1 (MQ=255) aaaCCCTGCGGCGGTCTGCTGGCAACGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGAGAAGCTATATCAATA‑TTAATCGCCCTGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTg > 1:441032/1‑251 (MQ=255) ggcggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGCTGAAATCGCAGATCCCAGCCAGCGTTGAGGTTTATCACGCCAGCCTGTGACGAAAAATATGGCGTggga > 2:563475/1‑247 (MQ=255) ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGgatgt > 2:679570/1‑250 (MQ=255) ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGgatg > 1:174010/1‑250 (MQ=255) tgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATgg > 1:531817/1‑251 (MQ=255) ggACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCtt > 2:533879/1‑152 (MQ=255) ggACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAACACTGGCGTGCGGATGCTAAATGGCATGTc > 2:278096/1‑250 (MQ=255) ggACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑GTAATCGCCATGCtt < 1:533879/152‑1 (MQ=255) ggACGCGCAGCGATCATTTATCTGCGATGGGCTGATGCTTCCGGGC‑GA‑GTGGATCCCTAATCCGCATATTTTCAGCGACAAAACCGTCGACGCCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAACCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCa < 2:129870/251‑1 (MQ=255) gACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCAt > 1:173259/1‑251 (MQ=255) aGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGTTAAATGGCATGTAATTTTTCgg > 2:502779/1‑250 (MQ=255) cGATTTTTTATTCGCGATGGGGTGACGCTTCCTGTC‑GATTGG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACGACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTGTCGACTTGTGGAGGAAATCGCTGACCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGc < 1:405337/251‑1 (MQ=255) aTCTTTTATTCGCGAAGGGCTGACGCTTCCTGTC‑GAAGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGACGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATTTCATTTTTCGTGccg > 2:390094/1‑249 (MQ=255) tttATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGc < 1:521299/245‑1 (MQ=255) tttATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACg < 1:319493/250‑1 (MQ=255) ggggaggggCGGACGCTCCCTGTT‑GATGTG‑ACCGCTAAACCGCAGATTTTCAGCGACAAACCCGCGGAGGGCGCCCCATCGCTAACGCGTATCTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGTTTTCGATTGGGGGATGAAAGCGCTGACCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCAGGTCATTTTTCGTGCAGACGGCTGGGa < 2:628186/245‑1 (MQ=255) cTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgc > 1:612867/1‑251 (MQ=255) cccTGTC‑GTTTTG‑ATCGCTAAGCCTCAGATTGTCAACTTCAAAACCGCAGCCGTCGCCCCACCGTTACCGCGCAACTACCAGAGAAG‑TTTAACAATAATTAATCGCCATGCTTCCGACTTTGGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGAGGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgccc < 2:496321/250‑1 (MQ=255) tGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCACAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTAACAGCGACGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGACATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAACCTGTGCCGCAAAATCTGGCGTAAGGAGGATAAATGGCCTGTCATTTTTCGTGCAGACGGCTGGGAgaaccataatcccgccagct > 2:179713/1‑232 (MQ=255) tGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTAGTGCAGACGGCTGGGAGCAACATATTAccgcccgtt > 2:385607/1‑249 (MQ=255) ggATGTG‑ACCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATc < 1:385607/250‑1 (MQ=255) gATGTG‑ATCGCTACTCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTAGTGCAGACGGCTGGGAGCAACATATTACCCACCGCTATCt > 2:509042/1‑251 (MQ=255) tg‑ATCGCTAATCCGCAGCTTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGCCAGCCTCTGCCGAACAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGCCGGCTGGGCGCCACATATTACCTCCCGCTATCTGGTc > 2:363070/1‑251 (MQ=255) g‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCg > 1:75880/1‑251 (MQ=255) g‑ATCGCTAACCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTACCTACCAGCGAAGCTATACCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTc < 1:140280/250‑1 (MQ=255) cGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTg > 1:506264/1‑251 (MQ=255) tAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTgacg > 2:485194/1‑248 (MQ=255) aaTCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGCGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGGGCCgc > 2:129903/1‑250 (MQ=255) tCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATg > 2:12515/1‑251 (MQ=255) cGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGGTATATCAATA‑TTAATCGCCAGGCTTTCGACTTGGGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGgc < 1:658894/251‑1 (MQ=255) cGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGgc > 1:14406/1‑251 (MQ=255) aTTTTCAGCGTCAAAACCGTCGCCGTCGCCGCTTCGCTAACGCGTCACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGCCAGCCTGTGCCGACAAATCTGGCGTGCGGATGATAAATGGCATGTCATTTTTCGTGCATCCGGCTGGGAGCAACATATTACCACCCGCTATCTGGTCGGTGCCGATggagcca > 2:312521/1‑245 (MQ=255) ttCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGcc > 2:511828/1‑114 (MQ=255) ttCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGcc < 1:511828/114‑1 (MQ=255) aaaaCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTgcg > 1:510470/1‑251 (MQ=255) cGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAtc < 1:679570/251‑1 (MQ=255) cATCGCTACCGCGTAACTACCAGCAAAGCTATAATAATA‑TGAACCTCAATGCTTTCGATTGGTGGATAAAATCGCTGACCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGat < 2:173259/251‑1 (MQ=255) cATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAACCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGat < 1:517929/251‑1 (MQ=255) | CGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTC‑GATGTG‑ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATA‑TTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGAT > NC_000913/3869396‑3869880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |