BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,761,230	G→A	S7F (TCC→TTC)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,761,230	0	G	A	99.0%	357.3 / NA	103	S7F (TCC→TTC)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (44/58); minor base C (1/0); total (45/58)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 103 total aligned reads displayed.

TTCCCGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGGAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGACG  >  NC_000913/3760985‑3761473

ttCCCGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAAcgc                                                                                                                                                                                                                                                  >  1:315834/1‑251 (MQ=255)

  cccGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGCCTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTAGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCCACGAAAGGAAGCAGCTATCGCGCAATAAGCGCTCAATAGCCGGAAGTTGACTATAGAGGAAACGCGt                                                                                                                                                                                                                                                >  2:690455/1‑251 (MQ=255)

  cccGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAAcgcg                                                                                                                                                                                                                                                 <  1:332806/250‑1 (MQ=255)

      tCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCg                                                                                                                                                                                                                                            >  2:187503/1‑251 (MQ=255)

       cAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTTCTTCGTCAACCGGGCATCGCCTTCTTGCGCCCAGTTTTCACACCACTCAGGCGGCGTCTGGCTGCCACGAATCACTGCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGTAAGTTGTCTATAGAGGAACCGCGTTTCgg                                                                                                                                                                                                                                           <  2:611400/251‑1 (MQ=255)

       cAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCAGCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCGCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGTGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCg                                                                                                                                                                                                                                            <  1:88898/250‑1 (MQ=255)

         ggTTGATCACCGGACTCAGCGCGCTCTGCGCTTCTTTCGTTAACCGGGCATCGAATTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTAGCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTGCGGtt                                                                                                                                                                                                                                         <  2:89462/251‑1 (MQ=255)

          gTTGATCACCGGCCGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGTCTTCTTGCGCCCAGTTTTCACCCCACGCAGGCAGCGTCTGGCTGCCACGAATCACTGCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTg                                                                                                                                                                                                                                        <  2:416279/251‑1 (MQ=255)

             gagcACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTc                                                                                                                                                                                                                                      <  1:376717/247‑1 (MQ=255)

               tCACCGGAAGCAGCGCGCTCTGCGCTTCTTTCGTCAACCAGACATCGACTTCTTGCGCCCAGTTCTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTCCGTCGAGAATCTGACGCAACAATTCCACCACGCGGGTGTGACAATAAGAAT‑ACAGCCAAGAAGGGAAGTAGCCATCTCGCAATAAGCGATCCATAGCCGGAGGATGACTATAGAGGAAACGCGTTGCAGTTGTCAt                                                                                                                                                                                                                                    >  2:688848/1‑250 (MQ=255)

                cACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATa                                                                                                                                                                                                                                   <  1:649734/250‑1 (MQ=255)

                     gACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGt                                                                                                                                                                                                                                          >  1:665263/1‑238 (MQ=255)

                     gACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGGCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGt                                                                                                                                                                                                                                          <  2:665263/238‑1 (MQ=255)

                      aCGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCg                                                                                                                                                                                                                                            <  1:348588/235‑1 (MQ=255)

                      aCGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCg                                                                                                                                                                                                                                            >  2:348588/1‑235 (MQ=255)

                       cGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATGCCACCACGCGGGTGTGACCATAAGTAGCAC‑GCAAAGAAAGGAAGGAGCTAGCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAAccg                                                                                                                                                                                                                           <  2:207831/251‑2 (MQ=255)

                          aGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCCTCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCAACCACGCGGGTGTGACCATACGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAGTAAGCGATCATTAGCAGGAAGTTGACTATCCAGGAACCGCGTTTCGGTTGTCATAGGAAACCTGGc                                                                                                                                                                                                                        >  2:367039/1‑251 (MQ=255)

                             gcgcTCAGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCCCGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGTCGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAA‑GCAAGGAAAGGAAGGAGCTATCGCGCAATAAGCGTTCAATAGCCGGAAGTTGACTATAGAGGACACGCGTTTCGGTTGTCATAGGAAACCTGGCTg                                                                                                                                                                                                                      >  2:178270/1‑250 (MQ=255)

                              cgcTCTGCCCTTCTTTCGTCAACCGGGGATCGACTTCTTGCGCCCAGTTTTCCCCCCACGCAGGCAGAGTCTGGCTGCCACGAATCACTGCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGAt                                                                                                                                                                                                                    <  2:338219/251‑1 (MQ=255)

                                ctctGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATc                                                                                                                                                                                                                   >  2:207656/1‑250 (MQ=255)

                                      gcTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGccc                                                                                                                                                                                                            >  1:275601/1‑251 (MQ=255)

                                            ttCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCCCTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCctct                                                                                                                                                                                                         <  1:606514/248‑1 (MQ=255)

                                                gcAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACgg                                                                                                                                                                                                  <  2:424734/250‑1 (MQ=255)

                                                   aCCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAAGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTAACACgg                                                                                                                                                                                                  >  2:313761/1‑248 (MQ=255)

                                                    ccGGGCAGCGACTGCTCGCGCACAGTTTCCACACCACGCAGTCAGCGTTTGGCTGTCAAGAATCACTTCCCGCGCTTCGTCGAGCAGCGGAAGCAACAATTCCACCACGCGGGTGGGACCATAAGTATAAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGGTGTCATAGGAAACCTGGCTGATCAAGGCCCTCGCTCATGGAGaa                                                                                                                                                                                              <  2:633030/251‑1 (MQ=255)

                                                              aCTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTa                                                                                                                                                                                     >  1:480463/1‑250 (MQ=255)

                                                               cttcttGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGCAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAAc                                                                                                                                                                                   >  2:524467/1‑251 (MQ=255)

                                                                      cgcCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACGTCTCGCTCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCa                                                                                                                                                                            <  2:551968/251‑1 (MQ=255)

                                                                       ccccatttgtCACACCACGCAGGCAGCGTCGGGCTGTCAAGAATCCCTGCTCGCGCTTCGTCGAGCAGCTGACGCCACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTACCACGGAGAAGGGCGTTTAACATAACCAc                                                                                                                                                                           <  2:183290/242‑1 (MQ=255)

                                                                        cccAGTTTTCACACCACACAGCCAGCGTCTGGCTGCCACGCCTCACTTCTCGCGCTTCGTCGACCCTCTGACGCAACAATTCCACCACGCGGGTGTGACCCTAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATAGCGCAATAAGCGATCGATAGCCGGAAGATGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACATGGCTGATCAAGGCCCTCTCCAACGGAGAAGGGCGTTGAACATAACCACg                                                                                                                                                                          >  2:413588/1‑251 (MQ=255)

                                                                         ccAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACgg                                                                                                                                                                         <  1:113639/251‑1 (MQ=255)

                                                                         ccAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACgg                                                                                                                                                                         <  1:249051/251‑1 (MQ=255)

                                                                                    cccacGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTg                                                                                                                                                              <  1:207656/250‑1 (MQ=255)

                                                                                         gcaggcagCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATg                                                                                                                                                         <  1:401815/251‑1 (MQ=255)

                                                                                            ggcagCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGt                                                                                                                                                      >  2:10792/1‑251 (MQ=255)

                                                                                               cgCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCag                                                                                                                                                   <  1:367039/250‑1 (MQ=255)

                                                                                                gcggcTGGCGGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCCCCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCagg                                                                                                                                                  <  2:160073/247‑1 (MQ=255)

                                                                                                gCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCagg                                                                                                                                                  >  1:450024/1‑251 (MQ=255)

                                                                                                 cGTCTGGCTGCCACGAAACACTTCTCGCGCTTCGTCGAGCATCGGACGCGACAATTCCACCACGCGGGTGTGACCATAAGTGTCAC‑GCATAGACAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGACGTTGCCTATAGAGGAAACGCGTTTCGGTTGTCATAGGCAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACCTAACCACGGATTGTAACGTCAGCTGGGTCaggg                                                                                                                                                 >  2:184668/1‑250 (MQ=255)

                                                                                                      ggCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGCGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGAc                                                                                                                                             >  2:124141/1‑250 (MQ=255)

                                                                                                      ggCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCaggagg                                                                                                                                               <  1:187503/248‑1 (MQ=255)

                                                                                                            cACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGGTGTCATAGGAAACCTGGCTGATCAAGGCCCGCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATcgc                                                                                                                                      <  1:326576/251‑1 (MQ=255)

                                                                                                                  tCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATc                                                                                                                                 >  1:189309/1‑250 (MQ=255)

                                                                                                                    ccTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAg                                                                                                                              <  1:124141/250‑1 (MQ=255)

                                                                                                                       tctcGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCt                                                                                                                           >  1:11353/1‑251 (MQ=255)

                                                                                                                           gcgcTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGACAGGAGGACATATCGCGCATCAAGCCTTTgg                                                                                                                       >  2:604756/1‑251 (MQ=255)

                                                                                                                            cgcTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGc                                                                                                                      >  1:390763/1‑251 (MQ=255)

                                                                                                                             gcTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCg                                                                                                                     >  1:2776/1‑251 (MQ=255)

                                                                                                                              cTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCa                                                                                                                                                    >  2:295434/1‑219 (MQ=255)

                                                                                                                              cTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCa                                                                                                                                                    <  1:295434/219‑1 (MQ=255)

                                                                                                                                 cgtcgAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGtt                                                                                                                  <  1:194899/250‑1 (MQ=255)

                                                                                                                                 cgtcgAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAAGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTc                                                                                                                 <  1:313761/251‑1 (MQ=255)

                                                                                                                                     gAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGCTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTg                                                                                                             >  1:230757/1‑251 (MQ=255)

                                                                                                                                           cTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAgg                                                                                                        >  2:60442/1‑249 (MQ=255)

                                                                                                                                                 aaacaaTTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCGCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCATTGGCGGTTCGGGGCGAGCAAAACtt                                                                                                 <  2:504443/250‑1 (MQ=255)

                                                                                                                                                  aacaaTTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGCAGTTGACTATAGAGGAAAAGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACttt                                                                                                >  1:460318/1‑251 (MQ=255)

                                                                                                                                                     aaTTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTcgc                                                                                             <  1:413588/251‑1 (MQ=255)

                                                                                                                                                      aTTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCTGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTcgcg                                                                                            <  1:30900/251‑1 (MQ=255)

                                                                                                                                                             cacGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAAc                                                                                    <  1:479389/251‑1 (MQ=255)

                                                                                                                                                             cacGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAAc                                                                                    >  1:126976/1‑251 (MQ=255)

                                                                                                                                                                gcgGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAgg                                                                                 <  1:84935/251‑1 (MQ=255)

                                                                                                                                                                 cgGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCTATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGCTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGt                                                                                <  2:230757/251‑1 (MQ=255)

                                                                                                                                                                  gggTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTTGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTc                                                                                                                 <  2:189697/218‑1 (MQ=255)

                                                                                                                                                                  gggTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTc                                                                                                                 >  1:189697/1‑218 (MQ=255)

                                                                                                                                                                    gtgtGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGAGTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGtttt                                                                             <  1:240757/251‑1 (MQ=255)

                                                                                                                                                                      tggACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGGTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTc                                                                            <  2:390763/248‑1 (MQ=255)

                                                                                                                                                                      gtgACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCg                                                                                                                     >  1:356296/1‑210 (MQ=255)

                                                                                                                                                                      gtgACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTGGTCATAGGAAACCTGGCTGAGCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCAGCAAGCCTTTGGCg                                                                                                                     <  2:356296/210‑1 (MQ=255)

                                                                                                                                                                        gACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑Ga                                                                         <  1:443849/250‑1 (MQ=255)

                                                                                                                                                                            aTAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGGTCGGTGCGAGAAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GAcccgt                                                                    >  2:608646/1‑248 (MQ=255)

                                                                                                                                                                             tAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAAc                                                                                                                                                                                   >  2:217074/1‑141 (MQ=255)

                                                                                                                                                                             tAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAAc                                                                                                                                                                                   <  1:217074/141‑1 (MQ=255)

                                                                                                                                                                             aaaGTATCAC‑GCAAAGAAAGGAAGGAGCTCTCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGtt                                                                   <  2:622809/250‑1 (MQ=255)

                                                                                                                                                                             aaaGTATCAC‑GCAAAGAAAGGAAGGAGAGATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACCATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCACTCGCACGGAGAAGGGCGTTTAACATAACCGCGGATTGTAACGGGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTGTGGCGGTTCGGTGCGAGCAAAACTGTCGCGTCT‑AAACAGGTTTGC‑GCCCAGtg                                                                   <  2:315834/250‑2 (MQ=255)

                                                                                                                                                                                  aTCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACt                                                              <  1:60442/251‑1 (MQ=255)

                                                                                                                                                                                    cAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTa                                                             <  1:252082/250‑1 (MQ=255)

                                                                                                                                                                                     aC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTGTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCGGTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAg                                                           <  1:85152/251‑1 (MQ=255)

                                                                                                                                                                                           aagaaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTgg                                                      <  1:456228/251‑1 (MQ=255)

                                                                                                                                                                                            agaaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTaa                                                                                                                                                                  <  2:184087/144‑1 (MQ=255)

                                                                                                                                                                                            agaaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTaa                                                                                                                                                                  >  1:184087/1‑144 (MQ=255)

                                                                                                                                                                                            agaaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTggg                                                     >  1:400093/1‑251 (MQ=255)

                                                                                                                                                                                            agaaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGCTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACAAGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGCTTTC‑GACCAGTTTGACTACGTGTggg                                                     >  1:28841/1‑251 (MQ=255)

                                                                                                                                                                                             gaaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGc                                                    <  1:608646/251‑1 (MQ=255)

                                                                                                                                                                                              aaagGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCCCGGATTGTAACGTTAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACGTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGCCTAAGTGTGGGCg                                                   <  2:198317/251‑1 (MQ=255)

                                                                                                                                                                                                  gaaggaGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTGTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCGTGCGGAGAAGGGCGTTTAACATAACCACGGATTTTAACGGGAGATGGGTCAGGAGGACATATCGCGCATCATGTCTTTGGCGGCTCGGTGCTAGCAAAACGTTCGCGGCT‑AAACAGGTTTTC‑GAACCGTTTGACTAAGTGTGGCCGGTCg                                               <  2:439678/251‑1 (MQ=255)

                                                                                                                                                                                                   aaggaGCGATCGCGCAATAAGCGATCAATAGCCTGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCAGAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTGTCGCGGCT‑AAACAGGTTTGC‑GACCAGTTTGACTAAGTGGGGGCGGTCGa                                              <  2:469479/251‑1 (MQ=255)

                                                                                                                                                                                                        gCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGacacac                                         >  1:538587/1‑251 (MQ=255)

                                                                                                                                                                                                           atctcgcAATAAGAGATCAATAGCCGGAAGTTGACTATAGAGGCACCGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCACTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACACATCGCGCATCAAGCCTTTGGCGGTTCGGTGGGAGCAAAACTTTCGCGGCT‑AAACATGCTTTC‑CACCAGTTTGACTACGGGAGGGCGGTCGACAAACCa                                       >  2:645806/5‑250 (MQ=255)

                                                                                                                                                                                                                         gATCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGACCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCACGCCTTTGGCGGTTCGGTGCGAGAAAAAATTTCGCGGCT‑AAACAGGTTTTCCGACC‑GTTTGACTAAGTGTGGGCGGTCGACACACCCACCCGgccaaaagcg                        >  2:618031/1‑243 (MQ=255)

                                                                                                                                                                                                                          aTCAATAGCCGGGAGTTGACTATAGAGGACACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCCCACGGAGAAGGGCGTATAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATAGCGCATCAAGCCTTTGACGGTTCGGAACGAGCAACACTTTTGCGGCTGAAAC‑GGGTTTC‑GACCCGTTTTACTAAGAGTGCGCGGTCTACACCCCAACCTGGCGCCAcgc                         >  2:685629/1‑249 (MQ=255)

                                                                                                                                                                                                                          aTCAATAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACgcgg                       >  1:272821/1‑251 (MQ=255)

                                                                                                                                                                                                                             aaTAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAg                                                           >  1:458703/1‑212 (MQ=255)

                                                                                                                                                                                                                             aaTAGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAg                                                           <  2:458703/212‑1 (MQ=255)

                                                                                                                                                                                                                                aGCCGGAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGCGAAGGGCGGTGAACATAACCACGGCTTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGTCACACCAACCTTGCGCAACGCGGCGGaa                  <  2:258711/250‑1 (MQ=255)

                                                                                                                                                                                                                                    ggAAGTTGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGGGCGAGCAAAACTTTCGAGGCT‑AAACAGGGTTTC‑GACCAGGTTGACTAAGTGTGGGCGGGCGACACACCAACCTGGCGCAACGCGGCGGAAATTgg             >  2:245625/1‑250 (MQ=255)

                                                                                                                                                                                                                                         ttGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGGGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGCAATTGAGatat        >  1:248853/1‑251 (MQ=255)

                                                                                                                                                                                                                                         gtGACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGatat        <  1:456056/250‑1 (MQ=255)

                                                                                                                                                                                                                                           gACTATAGAGGAAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATc       <  2:538587/250‑1 (MQ=255)

                                                                                                                                                                                                                                               ataGAGGAAACGCGTTTCGGTTGTCATTGGAAACCTGGATGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTGGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGACg  <  2:480463/251‑1 (MQ=255)

                                                                                                                                                                                                                                               ataGAGGAAACGCGTTTCGGTTGTCATAGGCAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTAGGTGCGGGCAAAAATTTCGCTGCT‑AAACAGGTTTTC‑GCCCCGTTTGACTAAGTGTGGGCGGCCCACACACCAACATGGCGCAAAGCGGCGGAAATTGAGATATCCGagg  >  2:303193/1‑249 (MQ=255)

TTCCCGTCAGGTTGATCACCGGACGCAGCGCGCTCTGCGCTTCTTTCGTCAACCGGGCATCGACTTCTTGCGCCCAGTTTTCACACCACGCAGGCAGCGTCTGGCTGCCACGAATCACTTCTCGCGCTTCGTCGAGCATCTGACGCAACAATTCCACCACGCGGGTGTGACCATAAGTATCAC‑GCAAAGAAAGGAAGGAGCTATCGCGCAATAAGCGATCAATAGCCGGAAGTTGACTATAGAGGGAACGCGTTTCGGTTGTCATAGGAAACCTGGCTGATCAAGGCCCTCTCACACGGAGAAGGGCGTTTAACATAACCACGGATTGTAACGTGAGATGGGTCAGGAGGACATATCGCGCATCAAGCCTTTGGCGGTTCGGTGCGAGCAAAACTTTCGCGGCT‑AAACAGGTTTTC‑GACCAGTTTGACTAAGTGTGGGCGGTCGACACACCAACCTGGCGCAACGCGGCGGAAATTGAGATATCCGACG  >  NC_000913/3760985‑3761473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`