Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	181.6 / NA	59	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/35);  total (24/35)

CGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTA  >  NC_000913/3387805‑3388284                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       cgcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGcaaca                                                                                                                                                                                                                                           >  1:675302/1‑250 (MQ=255)  gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCAAACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAt                                                                                                                                                                                                                                         <  1:100436/251‑1 (MQ=255)     gTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAACGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAAAAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGGATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATccc                                                                                                                                                                                                                                      >  2:571949/1‑249 (MQ=255)         aaCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCt                                                                                                                                                                                                                                  >  1:174392/1‑251 (MQ=255)          aCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAGTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTg                                                                                                                                                                                                                                 <  1:69157/251‑1 (MQ=255)            cGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTc                                                                                                                                                                                                                               <  1:434323/251‑1 (MQ=255)                 aGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTAc                                                                                                                                                                                                                          >  1:309346/1‑251 (MQ=255)                      ccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGccc                                                                                                                                                                                                                     >  2:356742/1‑251 (MQ=255)                        aCGCATACGAGATTTCTATTCGTACCAGCGAGGTTATCCAGGCACAAATACCGGCCCAGATACAGCACACCAGAAACATCAATAATGGTGCGCGGCTCATCGCCATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCACAAAGCCACGATAGCGAATAGCGCCAGCATACGGTTCACCTCCCGCAGCAACGGCCGGCCCGGCGGCAAAAATGGCCGCTGTCAGTGCCGCCCa                                                                                                                                                                                                                    >  1:32610/1‑250 (MQ=255)                            aTACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTg                                                                                                                                                                                                               >  1:354886/1‑251 (MQ=255)                                gAGTTTTCTATTCGTACCAGCGAGGATATACAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGCATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGt                                                                                                                                                                                                           >  1:209426/1‑251 (MQ=255)                                 aGTTGTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAAACATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAAGACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGtt                                                                                                                                                                                                          <  1:685391/251‑1 (MQ=255)                                                      aGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGGGGCGTTTCCAGCTGGAAGTGAAAGcc                                                                                                                                                                                      >  2:243248/1‑250 (MQ=255)                                                                       aaaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGAACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGTAAAGCCAACAAACAGCGcccagac                                                                                                                                                                    >  2:12281/1‑246 (MQ=255)                                                                              cccAGATACAGCACACCAGAATCACCAATAATGGGGCGCGGATCATCGAAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCg                                                                                                                                                             <  2:68136/251‑1 (MQ=255)                                                                                 agagacagcactaccgAATCATCAATAATGGTGCGCGGATCACCGCAATGATGATCACCAGTCCGCCAATACAGCCAATAAATGTGCCGATGATTCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTc                                                                                                                                                          <  1:28771/236‑1 (MQ=255)                                                                                    tACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGcc                                                                                                                                                       >  1:489862/1‑251 (MQ=255)                                                                                      cAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTg                                                                                                                                                                                            >  1:77449/1‑212 (MQ=255)                                                                                      cAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTg                                                                                                                                                                                            <  2:77449/212‑1 (MQ=255)                                                                                       aGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCAACAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCCCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGCTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGCCGCCAGt                                                                                                                                                    >  1:442108/1‑251 (MQ=255)                                                                                         cacaCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGACGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCCGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCACAAAACAGAGCCGGTACAATGGCGGGCGcccagtt                                                                                                                                                  >  2:153575/1‑246 (MQ=255)                                                                                            cccAGAATCATCAATAAGGGTGGGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAAGGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTAc                                                                                                                                               <  2:303692/250‑1 (MQ=255)                                                                                                  atcatcAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCaaa                                                                                                                                         <  1:502428/251‑1 (MQ=255)                                                                                                       aaatataggtgctcgaatattcGCAATGCTGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAACAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAGCGCCGCTGTCAGTACCGCCCAGCGTGGCGTGTCCAGCTGGAAGTTAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGaa                                                                                                                                    <  2:35099/231‑1 (MQ=255)                                                                                                       aaataatGGTGCGCGGATCAACGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAAGGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCGCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGGACAATGGCGGTCGCCAGTTTTACCGCAAAGCGaa                                                                                                                                    <  2:65100/250‑1 (MQ=255)                                                                                                               gTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTgg                                                                                                                            <  1:230997/251‑1 (MQ=255)                                                                                                                 gcgcGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGACGCCAGGTTTACCGCCAAGCGGATAAGTTGGtt                                                                                                                          >  1:343287/1‑251 (MQ=255)                                                                                                                 gcgcGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCACCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGt                                                                                                                           <  1:356742/250‑1 (MQ=255)                                                                                                                  cgcgGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTTCCGCAACACGAATATGTTgggt                                                                                                                          >  1:391891/1‑248 (MQ=255)                                                                                                                     ggATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCa                                                                                                                      <  1:12281/251‑1 (MQ=255)                                                                                                                      gATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCCGAAAGAGAAAATGTTGGTTAGCa                                                                                                                      >  1:202971/1‑250 (MQ=255)                                                                                                                        acatcgaaatgcagatCACCAGTCCGGCAATACAGCCAATAAATGGGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCGCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATg                                                                                                                   <  2:309346/238‑1 (MQ=255)                                                                                                                                atgatgATCACCAGTCCGGAAATACAGCCAATAAACGTGCGGATGATGCGCAAAAAGCCACGATAGAGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAAt                                                                                                          <  1:233205/251‑1 (MQ=255)                                                                                                                                     gatCACCCGTCCGGCAGTCCAGCCAAAAAATGTGCCGAGGATCCGCAAAAAGCCGCGATAGCGAATAGCGCCAGAATGCGGTTCACCTCCCGCAACAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACATCGGCGGTCGCCAGTTTTACCTCAAAGCGAATATGTTGGTTAGCAATTGA‑Gaaaaggccca                                                                                                     <  2:38066/251‑7 (MQ=255)                                                                                                                                      atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCAt                                                                                                   <  1:601908/251‑1 (MQ=255)                                                                                                                                          ccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTg                                                                                               <  1:500759/251‑1 (MQ=255)                                                                                                                                             gTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGAtt                                                                                            <  1:383693/251‑1 (MQ=255)                                                                                                                                                        cAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGTTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCa                                                                                 <  1:571949/251‑1 (MQ=255)                                                                                                                                                         agctcagaaaTGGGCCTATGATGCGCAAAAAGCCACGATAGAGTATAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCTGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAc                                                                                <  2:633671/244‑1 (MQ=255)                                                                                                                                                                         gatggtgaccAAAAAGCAACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGCCCGGCTGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTCCAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCAt                                                               <  2:80265/242‑1 (MQ=255)                                                                                                                                                                               ccccAAAAAGCACCGATAGCGAATTGCGCCAGTATACTGTGCCCCCCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAATACCGCCCAGCGTGGCGTTGCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGTTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CTCATCGTGATGAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACg                                                         <  2:396751/248‑1 (MQ=255)                                                                                                                                                                                   aaaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCACCAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCCGTACAATGGCGGTCGCCAGGTTTAACGCCAAACGAATATGTTGGTTAGCAATGGG‑GAAAATA‑CCCCTCCTGGTTAACCAAACTCAccccagcgctgggccagttttagga                                                        >  2:348511/1‑225 (MQ=255)                                                                                                                                                                                     aaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCGCAAAGCGAATAAGTTGGTTTACAATGGAAGAAAA‑A‑CCCAACGGGCTTAACCAAACTCACCGCAGG‑GATGGGGCCTTTTACGgaaga                                                    >  1:556116/1‑250 (MQ=255)                                                                                                                                                                                       aGCCCCGATAGCGAATAGCGCCAGAATGCGGGTTACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACg                                                 <  2:489862/251‑1 (MQ=255)                                                                                                                                                                                         ccACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCg                                                                                                 <  1:127736/202‑1 (MQ=255)                                                                                                                                                                                          cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTACCGCCAAGCGAAAATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGGTATTTAACAAACTCAC‑GCAGGCGATGTGCCATTTTAAGGAAGGACGaa                                               >  1:215971/1‑250 (MQ=255)                                                                                                                                                                                           aCGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCATCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATc                                             <  1:91524/251‑1 (MQ=255)                                                                                                                                                                                           aCGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATc                                             <  1:243248/251‑1 (MQ=255)                                                                                                                                                                                              atagcgcatgtatccAGAATACGGTTCCCCTCCCTCAGCAAAGGCCGGACCGGCGGCAACATCCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTTAAATCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTACCTCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATTCTg                                          <  2:280944/238‑1 (MQ=255)                                                                                                                                                                                              aTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGc                                                                         <  1:89622/220‑1 (MQ=255)                                                                                                                                                                                                   gAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGGCCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGt                                                                                                <  1:584901/193‑1 (MQ=255)                                                                                                                                                                                                     aTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcg                                   <  1:109172/251‑1 (MQ=255)                                                                                                                                                                                                            cAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAAACCTGGCTTTCGTCGCGAt                            <  1:611863/251‑1 (MQ=255)                                                                                                                                                                                                                aTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTc                                                                                                                                                          <  1:623420/124‑1 (MQ=255)                                                                                                                                                                                                                aTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGAAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGATTTACCGGAAAGCGAATATGTTGGTTTGCAATGGA‑GAAAATA‑CCCATCGTGGTTAACCAAAATCCC‑GCAGGCGATGGGGCATTTTACGGAAGAACGAATCCTGGcctttctcggggtattt                        >  1:205678/1‑235 (MQ=255)                                                                                                                                                                                                                      ttCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTTCCGCAAAGCGAATAAGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGGGATTAACCAAACTCAC‑GCAGGAGATGGGCCATTTTAAGGAAGAAAGAATCCTGGCTTTCGTCGCGGTCtttttttcc                  >  1:304648/1‑248 (MQ=255)                                                                                                                                                                                                                         aCCTCCCGCAGCAAAGGCCGGACAGCCGGCAACAATCCCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCCAAGGGCATATGTTGGTTAGCAATGGA‑GAAAATAACCCA‑CGTGATTAACCAAACCCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTAGTCGCGATCTTTTTTGCCacg               >  1:556917/1‑249 (MQ=255)                                                                                                                                                                                                                                    caAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCAACGTGATTAACCAAAATCAC‑GCAGGCGATGGGCCATTTTTAGGGAGAACGAATCCTGGGTTTCGTCGCGATCTTGTTTTCCCGTGACCACCACTg    >  1:542763/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTGCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCATTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTa  <  2:224014/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       CGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGA‑GAAAATA‑CCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTA  >  NC_000913/3387805‑3388284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑