Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	181.4 / NA	56	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/27);  total (29/27)

GTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAA  >  NC_000913/2484583‑2485069                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   gttgCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACg                                                                                                                                                                                                                                              >  1:693657/1‑251 (MQ=255)    gCATACCGAATCGCAGCAACGGGTTCGTGGGATTAATGCTGATTATTTAAATCTTTCAAAAAGATCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTACATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTATCCACCTTTCACGAc                                                                                                                                                                                                                                            <  2:382151/250‑1 (MQ=255)          cGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGCCGCTGGTAACCACCTTTCACGACTCAATGc                                                                                                                                                                                                                                     >  2:432384/1‑251 (MQ=255)                  agcaACGGGTTCGTGGTATTAATGATGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCtt                                                                                                                                                                                                                             <  2:109771/251‑1 (MQ=255)                     aactagTTCGAGGTATTAATGCTGATGATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTACGGAAATACGCAGCTCATCAAAAAGCAATGGAAGAGCTTGCAGAAGGAGAAGGCGATATAGTGATATCACATTTAGTTACTTCGCAGCATCTTAATAATGACATTGATGCAACAAAACCATTGATAATTATCTTTCCGTCGCTGGTAACCACCTTTCACGaatacaagcgaaagattaac                                                                                                                                                                                                                          >  2:372460/6‑232 (MQ=255)                                   aTTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAACACCAGt                                                                                                                                                                                                            >  1:221853/1‑251 (MQ=255)                                       aTGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTACGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTATCGACTCAATGCGACCGCTTCCCACACCAAAACCAGTAAat                                                                                                                                                                                                        <  2:615138/251‑1 (MQ=255)                                         gCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGAAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACAATTGATAATTCCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTaataat                                                                                                                                                                                                      >  2:223328/1‑247 (MQ=255)                                               tattTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCg                                                                                                                                                                                                >  2:186622/1‑251 (MQ=255)                                                          tttAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAAtt                                                                                                                                                                                     >  1:181835/1‑251 (MQ=255)                                                              aaaaGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAg                                                                                                                                                                                           >  2:250316/1‑241 (MQ=255)                                                              aaaaGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAg                                                                                                                                                                                           <  1:250316/241‑1 (MQ=255)                                                              aaaaGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccc                                                                                                                                                                                 <  1:640538/251‑1 (MQ=255)                                                                 agagCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAACAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGATCGGGTAGCAAATTAcccccc                                                                                                                                                                              >  1:463107/1‑251 (MQ=255)                                                                          aaTATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGt                                                                                                                                                                     >  2:229518/1‑251 (MQ=255)                                                                                                aaTACGCAGATCATCAAAAATCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATTACATTGCTGCAACCAAACCATTGAGAATTACCTTGTCGGCGCTGGTAACCACCTGTCACTACTCAAGGCGGCCGCTTACCCCTCCAAAACCAGTAAATACTGCTCGGGTAGCAAATGACCCCCCAGACGAGGTAATTCATCAATCATTTCCaaaa                                                                                                                                               <  1:625015/251‑1 (MQ=255)                                                                                                      cAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaac                                                                                                                                          >  1:667300/1‑250 (MQ=255)                                                                                                       aGATCAACAAACAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATCATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCACAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGCGGTAATTCATCAATCATTTCCAAAAGcaaca                                                                                                                                         >  1:39922/1‑250 (MQ=255)                                                                                                          acatcaAAAAGCAATGGACGCCCTCGCACAAGGGGAAGTAGATAATGTTTTATCTAATCTAGTTTCTTCTCCGCCTCTTAATAATGACATTGCTGCAACCAAACCGTTGATAATTACCTTGCCGGCGCTGGTAACCACCTGTCACGACTCAATGCGACCGCGTACCTCACCAAAACCATTAAATATTGCTCTGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATTATGTCCAAAAGCAACAATTa                                                                                                                                     <  2:318897/250‑1 (MQ=255)                                                                                                              caAAAAACAATTGACGAGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACAATTAGTTACTTCGCCGCCTCTTAACAATGACATGGCTGCAACCAAACCATTGATAAGTACCTGTCCGGCGCTGGTAACCACCTTTCACGGCTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCGCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCAGCAATCCTTTCCAAAAGCAACAATTAtctc                                                                                                                                 <  2:39922/251‑1 (MQ=255)                                                                                                                     cAATGGACGCGCTTGCAGCAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc                                                                                                                           >  2:642955/1‑250 (MQ=255)                                                                                                                       aTGGACGCGCTTGCTGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACAGTGCTGCAACCAAACCATTGATAATTACCCTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCAGCAATCATTTCCAAAAGCAACAATTATCTCTTTTACaaa                                                                                                                        <  2:597413/251‑1 (MQ=255)                                                                                                                       aTGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTCTCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTCCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGAAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                                                                  >  2:679914/1‑241 (MQ=255)                                                                                                                       aTGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                                                                  <  1:679914/241‑1 (MQ=255)                                                                                                                           aCGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCGCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACTCCCCAGACTAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTta                                                                                                                    <  1:368230/251‑1 (MQ=255)                                                                                                                           aCGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTta                                                                                                                    <  1:454732/251‑1 (MQ=255)                                                                                                                                  tGCAGAAGGTGAAGTCTATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTGAATAATGTCATTGCTGCAACCAAACCATTGATAATGACCTTTCCGGCGCTGGTAACCACCTTTCACGACGCAATGCGACCTCTTACCTCACCAAAACCAGTAAATATGGCTCGGGTAGCAAATTACCCCCCa                                                                                                                                                                             <  2:57300/187‑1 (MQ=255)                                                                                                                                  tGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCa                                                                                                                                                                             >  1:57300/1‑187 (MQ=255)                                                                                                                                         ggTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGc                                                                                                                                                                                                                                     >  2:485020/1‑124 (MQ=255)                                                                                                                                         ggTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGc                                                                                                                                                                                                                                     <  1:485020/124‑1 (MQ=255)                                                                                                                                                 cgctataGTGTTATCACATTTAATTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCTGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTCTATCTTTTACAAATTAATATAAGGCATTAGCATCCGtct                                                                                              >  2:222546/4‑251 (MQ=255)                                                                                                                                                  gATATAGAGTTATCACATTTAGTTACTACGCCGCCTCTTAATAATGACATTGCTGCAACCAAAACCTTGATAATTACATTTCCGGCGATGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCACAACCAGTACATATTGCTCGGGTAGCGAATTACCCCCCCGACGCGGTAATTCTTCAATCATTTCCAAAAGAAACAATTATCTCTTTTACAACTTTATATCAGGCATTAACATCCGtctc                                                                                             >  2:415454/1‑251 (MQ=255)                                                                                                                                                     ataGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATACGTCTCAGc                                                                                          >  1:422117/1‑251 (MQ=255)                                                                                                                                                        gtgtTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTgg                                                                                       >  1:87321/1‑251 (MQ=255)                                                                                                                                                        gggtTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACGCAAGGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCCCTTTTACAAAATTATATCAGGCATTAGCATCCGTCTCAGCTgg                                                                                       <  2:181835/249‑1 (MQ=255)                                                                                                                                                          gtTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTGCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                                                                     <  1:604597/251‑1 (MQ=255)                                                                                                                                                           ttATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACCTTGCAGCAACCAAACCATTGTTAATTACCTTTCTGGCGCTGGTAACCACCTTTCACGACTCAATGCGCCCGCTTACCTCACCaaa                                                                                                                                                                                                                  >  1:604147/1‑125 (MQ=255)                                                                                                                                                           ttATCACATGTAGTTTCTTCGCCGCCTCTTAAGAATGACATTGCTGCAACCAAACCATTGATAATGACCTTTCCGGCGCTGGTAACCACCTGTCACGACTCAATGCGACCGCTTACCTCACCaaa                                                                                                                                                                                                                  <  2:604147/125‑1 (MQ=255)                                                                                                                                                                   tttAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGTCACAATGAtt                                                                            >  1:416518/1‑251 (MQ=255)                                                                                                                                                                                   ccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa                                                            <  1:642955/251‑1 (MQ=255)                                                                                                                                                                                      ctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat                                                         >  1:642846/1‑251 (MQ=255)                                                                                                                                                                                        taataaTGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat                                                         <  1:411021/249‑1 (MQ=255)                                                                                                                                                                                        taataaTGACATTGATGAAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCACTGCGACCGCTTACCTCACAAAAACCAGTAAATATTGCTCGGGAAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAACAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGACTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcatc                                                        >  2:654776/1‑250 (MQ=255)                                                                                                                                                                                          ataatGACATTGCTGCAACAAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGAATCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCAtt                                                     >  2:654085/1‑251 (MQ=255)                                                                                                                                                                                           taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCAtt                                                     >  1:396997/1‑250 (MQ=255)                                                                                                                                                                                                     gctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTAt                                          <  1:619990/251‑1 (MQ=255)                                                                                                                                                                                                             caaaccaTTGATAATTACCTTCCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAACAGCAACAATTATCTCTTTTACAAATTTATATAAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTTTGATTTccc                                  >  2:233462/1‑251 (MQ=255)                                                                                                                                                                                                                accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGa                                                                                                                                                                           >  1:104944/1‑111 (MQ=255)                                                                                                                                                                                                                accaTGGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCGCACCAAAACCAGTAAATATTGCTCGGGTAGCAAAATACCCCCCAGa                                                                                                                                                                           <  2:104944/111‑1 (MQ=255)                                                                                                                                                                                                                  caTTGATAAGTACCTTTCCGGCTCTGGTAACCACCTTTCACGACGCAATGCGACCGCTTACCTCCCCAAAACCAGTAAAAAGTGCCCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCAACAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATTTTTACTTAATTGGTAGTAACATCATTGCCAGCAGTATGATTTCCAGCTAt                             <  2:468948/251‑1 (MQ=255)                                                                                                                                                                                                                   aTTGATAATGACCCTTCCGTCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTGTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGGAACATCATTACCAGCAGTATGATTGCCCGCTAtt                            <  2:642846/251‑1 (MQ=255)                                                                                                                                                                                                                   aTTGATAAGTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTAtt                            <  1:460755/251‑1 (MQ=255)                                                                                                                                                                                                                            ttccTTTCCGGCGCTGGTAACCCCCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGTGTAGCAAATTTCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAATCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactctc                    <  2:487108/248‑3 (MQ=255)                                                                                                                                                                                                                                tttCCGGCGCTGGGAACCACCTTTCACGACTCAATGCGCCCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGAAGCAAATTACCCCCCAGACGAGGAAATTCATCAATCATTTCCAAAAGCAAAAATTATCTCTTTTACAAATTTATATCAGGCATTAACAACCGTCTACGCTGGGCAAAATAATTACTTTATTGGAAGTAACATAATTACCAGCAGTATGATTTCCCGCTATTTAACTCACTCCtt               >  2:581223/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACGCACTCCTTAAATGTAGt      <  1:186622/249‑1 (MQ=255)                                                                                                                                                                                                                                              aaCCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGaaa  <  1:354096/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAA  >  NC_000913/2484583‑2485069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑