| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 65,811 | G→T | intergenic (‑31/+44) | polB ← / ← araD | DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 65,811 | 0 | G | T | 96.7% | 184.1 / ‑0.4 | 60 | intergenic (‑31/+44) | polB/araD | DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (27/31); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01 | |||||||||||
TAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑AAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATC‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAAC > NC_000913/65571‑66055 | tAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑AAAACGTAACCCGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCCGTGTCGGGGTAAAA‑TAAAACCTGCCTGCGCCACGCGGTAATTTCCA‑CAAAAAACCAGGCTTTATTATAGcccgg > 1:326304/1‑248 (MQ=255)tAACGCCAGCGGTGTCAGGCGAACGCCTTGTTAACCCTGCAAAATATGCGGAGCGCGGGGAACCTGACCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTg < 1:483051/250‑1 (MQ=255) cAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGACCGGCGGGAATAAACGCCACGGACTCTGGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGttt < 1:655918/248‑1 (MQ=255) gatcgCCTTGTGCACCCTGAAAAATTGGCTGGGCGCGGGGAACCGGATCGGCGGGAATAAACGCCACGGCCGCGTGTGGGG‑AAAGGTTAACCTGCAACGGCCCGTTGGCCGGCGCCAGCAAGAAGGAGATTTCTGGCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAATCCGGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTgg < 2:688177/247‑1 (MQ=255) gaacgCCTTGTTCCCCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTgg < 1:601165/247‑1 (MQ=255) gaacgCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTgg < 1:20548/247‑1 (MQ=255) gtCACCCTGCAAAATATGCGGAGCGCGGGTAGCCTGATCGGCGGAAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCTTTGTCCGTCGCCAGCCAGAAGGAGACTGCTGTCCCT‑GGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAg < 1:109550/250‑1 (MQ=255) ccccggcaaaatatgtggggggcgGGGAACCTGAGCGGGGGGAATAAACGCCACGGACTCTGGCGTGG‑CAAGCGTAACCGGAAACGGCCCGTTGTCCGTCGCCAGCCAGAGGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCa < 2:677838/230‑1 (MQ=255) ccggaaaaaTATGCGGGGCGCGGGGAACGTGTCCGGGGGAAATAACCGCCCTGGCCTCTTCGGGGG‑CAAGCGTAACTTGCACCGGCCGGTGGTCCGCTGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGTTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCAtt < 2:75618/246‑1 (MQ=255) agccgtgagctcttccgatcgggggAGCCTGATCGGCGGGAATAAACGCCACGGACGCTTG‑GGTGGCAAGCGAAGCATGCAACGGGCGGTTGTCCGTCGCCAGCAAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGCCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCAtt < 1:74293/230‑1 (MQ=255) tGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGGTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCCGTGTCGGGTTTAGA‑TAAAAACTGCCTGCGCCCCCCTG‑GAATTTCATCAAAAAACCAAGCTTGATTTTATCCTGGGTTTGTTTGTTTTTCTGTGGGTTTTTACAGttcttattggccgta > 1:521394/1‑236 (MQ=255) gCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTaa < 1:64940/250‑1 (MQ=255) cTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGG‑CAAACGTAACCTGCAACGGCCCGGTGGCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCC‑TGCGGGGTGGCCCGCCCGTGTCGGGTTAAGAGAAAAACCT‑CCTTCGCCACCCAG‑TAATTTCATCAAAAAAACAGGCTTTATTATAACCTGGGTTCGTTTGATTGGGTGGGGTTTTATACAggtattaattccccttat > 1:501655/1‑233 (MQ=255) gggcggGAATAAACGCCACGGACTCTGGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTAc < 1:277487/250‑1 (MQ=255) ggcggAAATAAACGCCACGGACTCTGGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTGGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACg < 1:323794/251‑1 (MQ=255) aaaCGCCACGGACTCTTGCGGTG‑CACGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGCGTCCCGCCAGTGACGGGGTAAGA‑TAAAACCTGCCTGCGCCCCGCTG‑TAATTCCATCAACAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGGGGTTTTATACATTCCTTACTGCCCGTAATAACACTTCGCGCCATGCTTACgacacaaggg > 2:368701/1‑242 (MQ=255) aaaCGCCACGGACTCTGGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGGCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTta < 1:705372/198‑1 (MQ=255) ccGCCACGTACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCTT‑TGCGGGATGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGtt < 1:121297/250‑1 (MQ=255) gCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGtttt > 1:134433/1‑250 (MQ=255) aCGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCtt > 1:631855/1‑220 (MQ=255) aCGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTAttc > 1:473885/1‑249 (MQ=255) caaacGTAACCTGCAACGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑a < 1:587924/247‑1 (MQ=255) cAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGGTTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCtt > 1:368622/1‑204 (MQ=255) aaCCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCgg < 1:89367/251‑1 (MQ=255) gCAACGGCCCTTTGTCCTGCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTTTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTaa < 1:303213/249‑1 (MQ=255) tcccgatcttggcccGTCCCCAGCCAGAAGGAGACTTC‑GGCCCTGTGCGGGTTGTCCCGCCATTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTg < 2:444271/237‑1 (MQ=255) cccGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCCCGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATACCCTGGTTTCGTGTGATTGGCTGCGGTTTTATACAGCCATTACTGCCCCTAATATGCCTTTGCTCCCTGCTTCAGC‑AGATAGACTTTATT‑CAGCAGCGATCGCTGCCAT‑TCCGGCAACTGCGgcgc > 1:42895/1‑251 (MQ=255) ttGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTg > 1:444271/1‑241 (MQ=255) gTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTg > 1:376790/1‑251 (MQ=255) cccGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGa < 1:533309/250‑1 (MQ=255) aGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGGTAAGA‑TAAAACCTGCCTGCGCCCCGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCCAGTTTCGTTTTATTTGCTGTGGTTTTATAAAGGCATTACTGACCGTAATATGCCTTCGCGCCATTCTTTCGCCAG‑TAGTTTTTTTTTCA‑CAGCTGTTGCTGC‑ATATCCGGGTACTTCGGCGCTAACTGACGGCAGaaattccccc > 2:458397/1‑243 (MQ=255) aGGAGACTTCTCTCCCT‑TGCGCGGTGTCCCCACAGCGTCGGGTTAAGC‑TAAAACCTGCCCTCGCCACGCAG‑CAATTCCCTCACCAAACCAGGCTTGATTATAGCCTGGTTTCGCTTGAATGGCTGTGGTTTTATAAAATAATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑ATATAGAGTTTATT‑CAGAAGCGTTTGGTGC‑ATCTCCGGTAACTGCGGCGCCAACAGAAGGCAGAatttcaccc > 2:653594/1‑244 (MQ=255) gTCCCT‑TGGGGGGTGACCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGGGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGa < 1:400670/249‑1 (MQ=255) gTCCCT‑GGGGGGGTGTCCCGCCAGTGGCGGGTTAAGA‑TAAAACCGGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGAcc < 2:114550/251‑1 (MQ=255) gggggTGCCCCGACAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAg < 2:276447/250‑1 (MQ=255) gggTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTTTT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGc > 1:476957/1‑250 (MQ=255) tgtCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACg > 1:35960/1‑250 (MQ=255) cccGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑AAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATC‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATgg < 1:39847/251‑1 (MQ=255) cccGCAAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTTATTATAGCCTGGTTGCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTGCCAGCACGATgg < 1:572596/251‑1 (MQ=255) ccGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAatat < 1:309577/129‑1 (MQ=255) ccGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAatat > 2:309577/1‑129 (MQ=255) cacgtggagggTTACGA‑TAACACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCGTGATTATAGACTGTGTTCTTTTGATTGGCTGTGGTTTTATACAGGCATTACTGCCCGTAATATGCCGTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCAGATAAGCGTCCTCTTCCAGCGCGATGGCGtt < 2:119869/243‑1 (MQ=255) tgtCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑AAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATC‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGc > 1:214703/1‑251 (MQ=255) gTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGc > 1:707532/1‑250 (MQ=255) ttAAGA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCAt > 2:143621/1‑251 (MQ=255) aaGA‑TAAACCCTTCCGGCGCCACGCTG‑TAATTCCATAAAAAAACCAGGCGTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCCCCGCATcg < 2:642351/251‑2 (MQ=255) gA‑TAAAACCTGCCTGCGCCACGCTG‑TAATTCCAGCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTc < 1:466218/251‑1 (MQ=255) aCCTGCCTGCGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACCGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAtt > 1:265468/1‑251 (MQ=255) ctgcctgcGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGCATATCCCCATATAAGCGACCTCTTCCAGCACACTGGCGTTATGCACCGCATCTTCGGCTtttt > 2:147062/1‑251 (MQ=255) tgcctgcGCCACGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATCTAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAttttt > 2:291300/1‑251 (MQ=255) cGCTG‑TAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaa > 1:342363/1‑250 (MQ=255) gctgtaattCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaaat < 1:680083/243‑2 (MQ=255) gtaattCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGACAGAGGTTTTATACATTCATTACTGCCCGTAATATGCCTTCGCTCCATGTTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCAGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATACGCGACCTCTTCCACCACGAAGGAGTTATGCACCTCATCTTCGGCCTTTTTGcccaagcaaatggg > 2:274872/6‑240 (MQ=255) cAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGGTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGaa < 1:570850/250‑1 (MQ=255) aaaaaaCCAGGCTTGATTATAGCCTGGTTTCGTTTGTTTGGCTGAGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGA‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGACTATCCCCCTATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCTCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTgagactg > 2:362852/1‑245 (MQ=255) aaaCCAGGCTTGATTATATCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCAGTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGAc < 2:379925/251‑1 (MQ=255) aGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAgg > 1:37232/1‑249 (MQ=255) ggCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCa < 1:645006/211‑1 (MQ=255) ggCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCa > 2:645006/1‑211 (MQ=255) ggCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGATTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATT‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAggac > 2:307966/1‑248 (MQ=255) | TAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTG‑CAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCT‑TGCGGGGTGTCCCGCCAGTGTCGGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑AAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGC‑AGATAGTGTTTATC‑CAGCAGCGTTTGCTGC‑ATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAAC > NC_000913/65571‑66055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |