Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,310,770	A→G	H32H (CAT→CAC)	yciI ←	protein YciI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,310,770	0	A	G	98.1%	176.4 / ‑4.3	54	H32H (CAT→CAC)	yciI	protein YciI
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (26/26);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

AACACGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTG  >  NC_000913/1310526‑1311015                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aaCACGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGt                                                                                                                                                                                                                                                 >  2:669796/1‑251 (MQ=255)    acGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGAAGTAAc                                                                                                                                                                                                                                              >  2:628849/1‑251 (MQ=255)         ttcAAAAACCGTCCCCTTAAATATTGAATCTCTATTCATAACTCTTTCTTAAATGTTTTCACTGAAACGTGGTTATAGACTCCTGCCGCTACGTACGGGTCAGCATCTGCCCAGGTCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTa                                                                                                                                                                                                                                          <  1:655935/248‑1 (MQ=255)              aaaCCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTg                                                                                                                                                                                                                                    >  2:676309/1‑251 (MQ=255)              aaaCCGTGCCATTAAATATGGAATCTATATACAGAACCCTTTCTTAAATGGTTTCACTGAAACGTGTTAAAAGACCCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTg                                                                                                                                                                                                                                    <  2:72124/251‑1 (MQ=255)                     ccccTTAATTATTGAATCTCTATTCAGAACCCTTTCTTAATTGGTTCCACTGAAACGGGTCCATAGACACCTGCCGCTTCGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAAtg                                                                                                                                                                                                                             <  2:343624/250‑1 (MQ=255)                         ttAAATATTGAATATCTATCCAGAACACTTTCTTAAATGGTTTCACTGAAACGCGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTTGCTCGTTACTGTCTACCGCTGGCATTGGTACAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTCAACGTGCTCAATGTGcc                                                                                                                                                                                                                         >  2:321969/1‑251 (MQ=255)                            aaTATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCgg                                                                                                                                                                                                                       >  1:280186/1‑250 (MQ=255)                                  accgctcttcaaatctcacTTTATTAAAGGGTTTACCTGAAACGTGTTAATAGACGCCGGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACg                                                                                                                                                                                                                <  2:633434/235‑1 (MQ=255)                                             cAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCCTCGGCCCAGGACTGCGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGCGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGCCCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAACGGCGttt                                                                                                                                                                                                     >  1:37993/1‑251 (MQ=255)                                                  cacTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAACCGTGCTAAATGTGCCGGACGAACg                                                                                                                                                                                                                >  1:633434/1‑235 (MQ=255)                                                       tctaaaagggTTCCACTGAAACGTGTTCAGAGTCGCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGGTGAGCCAGTAAATCCCGCAGCCCCTGGAGCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTGTTTCGAGGCt                                                                                                                                                                                            <  2:452587/242‑1 (MQ=255)                                                          tAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTAt                                                                                                                                                                                          <  1:625285/249‑1 (MQ=255)                                                                 tttCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGt                                                                                                                                                                                 >  1:185395/1‑251 (MQ=255)                                                                  ttCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGt                                                                                                                                                                                 <  1:228808/250‑1 (MQ=255)                                                                   tCACGGAACCGTTTTCATAGCCTCCTGCCGCTACGGACGGGGCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTc                                                                                                                                                                                <  2:604373/250‑1 (MQ=255)                                                                    cACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCtt                                                                                                                                                                              >  1:555413/1‑251 (MQ=255)                                                                       tGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGCGGCTATCAGCTTTGTCTTg                                                                                                                                                                             >  1:216703/1‑249 (MQ=255)                                                                            cgggtTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTaa                                                                                                                                                                      <  1:17063/248‑1 (MQ=255)                                                                            cGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTa                                                                                                                                                                       >  1:269465/1‑250 (MQ=255)                                                                                  cATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGGGCGTAAATAAc                                                                                                                                                                 >  1:553577/1‑250 (MQ=255)                                                                                        cTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAACTGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATacaa                                                                                                                                                           >  2:40372/1‑250 (MQ=255)                                                                                          ccTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATacaac                                                                                                                                                          <  1:669796/249‑1 (MQ=255)                                                                                             gCCGCTACGTACGGGTCCGCATCGGCCCAGGCCTGTGCTGCTGCCAGCGATTCAAATTCATCCATAACGGTTGCGCCAGTAAAGCCCGCAGCCTCTGGATCGTTTCTGTCTAACGCTGGCATTTTACCAGCTGTCAACAATCGACCTTCATTGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTGGTCTTGAGCTTAAATAACATACAACACg                                                                                                                                                       <  2:540301/249‑1 (MQ=255)                                                                                                  tacttccgGGTCAGCATCGTCCCATGTCTCAGCTGTTTCCATCGATTCATATTCCGCATTAACGTTTGAGTCCTTAAACACTGCAGTCCCTGGTACGTTTCTATCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCCTCATCTTGCACTACCTGTAACCGCGTTAAATGTGCCTTACGCACGGAATGTCGTTTTTCGATGCTATCAGCTTTGTCTTGATCGTAAATAACATaccccacgcccaca                                                                                                                                                 <  2:543023/244‑13 (MQ=255)                                                                                                                      cccAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTg                                                                                                                            <  1:53009/251‑1 (MQ=255)                                                                                                                             cTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGAACAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTCTCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGTGAAAGTTGTAAAGt                                                                                                                      >  1:331847/1‑250 (MQ=255)                                                                                                                              tGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTAc                                                                                                                    >  1:176110/1‑251 (MQ=255)                                                                                                                               gAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTAc                                                                                                                    <  2:185395/250‑1 (MQ=255)                                                                                                                                aGCTGCTTCCAGCGATTCAAATTCAGAAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGAAACTGTAAACGTGCTAAAAGTGCCGGACGAACGGAACGGCGTTTTTAGAGGCTATAAGCTATGTCTTGAGCGTAAAAAACAAAAAACCCGGGAACAACTCCTTGTTCGGGCAAGTTGTaaataaagt                                                                                                                  >  2:494468/1‑245 (MQ=255)                                                                                                                                                   aaTTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGCCCTTCATCGTGCAGTAACTGTAACCGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTCAAGAACGTTATTTGAAAGGGCGa                                                                                                   >  2:477671/1‑247 (MQ=255)                                                                                                                                                       cagaaaaaacagTTGAGCCAGTAAATCCCTCAGCTGCAGGATCGTTACTGGCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCGTCATCGTGCAGTAAATGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTGGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGAGCTg                                                                                            <  2:141951/239‑1 (MQ=255)                                                                                                                                                       cAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGc                                                                                           >  1:233267/1‑251 (MQ=255)                                                                                                                                                        aGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACGGTCTACCGCTGGCATTGGACCAGCTGGAAACAAACGACCTTTATCGTGCAGTAACTGTAAACGTGCTAAATGGGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCa                                                                                          <  2:516198/251‑1 (MQ=255)                                                                                                                                                              aaCGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGa                                                                                     <  1:40372/250‑1 (MQ=255)                                                                                                                                                              aaCGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACCCGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGa                                                                                     >  1:80242/1‑250 (MQ=255)                                                                                                                                                                  gTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAgat                                                                                <  1:92906/251‑1 (MQ=255)                                                                                                                                                                           ggaaaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCttt                                                                       <  2:329810/249‑1 (MQ=255)                                                                                                                                                                           gTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCttt                                                                       <  1:676309/251‑1 (MQ=255)                                                                                                                                                                             aaaTCCCGCAGCCCCTGTAATGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGt                                                                     <  2:499635/251‑1 (MQ=255)                                                                                                                                                                              aaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTg                                                                                                                            >  2:85869/1‑195 (MQ=255)                                                                                                                                                                              aaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTg                                                                                                                            <  1:85869/195‑1 (MQ=255)                                                                                                                                                                                        ccccTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGc                                                          <  1:247550/251‑1 (MQ=255)                                                                                                                                                                                             ggagcGTTCCTGTCTAACGCAGGCATTGGACCAGTTGGCAACAAACGACCGTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTGTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACTCGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTATGTCCATGCATa                                                       <  2:303732/245‑1 (MQ=255)                                                                                                                                                                                              gATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATaa                                                      >  1:39024/1‑249 (MQ=255)                                                                                                                                                                                                    tACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAGGTAAggg                                              >  1:143124/1‑251 (MQ=255)                                                                                                                                                                                                                 tGGCATTTGACCAGCTGTCAAAAAACGACCTTCATCGTGCAGTGACTGTAAACGTGCTAAATGTGCCGGACTAACGGACAGGCGTTTTTCGATGCTATCAGCTTTATCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGTGAAATTTGTAAAGTACGTTATTAGAACGGACGACGATCTGCAACGCAAAGATGATGTTTTTGTTAAGTCCATGCTTAAAGTAAgaataataccgcaaa                                  >  2:25526/1‑236 (MQ=255)                                                                                                                                                                                                                  ggCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCaaa                                 <  1:668762/250‑1 (MQ=255)                                                                                                                                                                                                                      ttGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATg                             >  1:227579/1‑250 (MQ=255)                                                                                                                                                                                                                         gACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAACCGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAACGTTGTAAAGTACGTTATTTGAAAGGGCGCAGATCTGCAACGGAAAGATGATGGCTTTGTTAATGCCATGCATAAAGTAAGGGTAATTAAGCCAAAAATGACAt                         >  2:468873/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGTGTAATTACGCCAAAAATGACATTTTCAc                   <  1:240894/249‑1 (MQ=255)                                                                                                                                                                                                                                 gTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCAct                  <  1:468873/250‑1 (MQ=255)                                                                                                                                                                                                                                        aaCGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAACTGACATTTTCActgatcctg           >  2:243335/1‑250 (MQ=255)                                                                                                                                                                                                                                           gACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCg       >  1:365652/1‑251 (MQ=255)                                                                                                                                                                                                                                                 catcgtGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTGTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTg  <  2:280186/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AACACGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTG  >  NC_000913/1310526‑1311015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑