Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	152.8 / NA	55	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/31);  total (24/31)

GGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCC  >  NC_000913/1306492‑1306977                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ggTAGAACTGGGTAACTATGATGAGGTCTACCACAATCCACTACAGCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAACCCAGCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGttt                                                                                                                                                                                                                                               <  1:25079/251‑1 (MQ=255)         tGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                                       >  1:249092/1‑251 (MQ=255)                            cTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTaa                                                                                                                                                                                                                     >  1:379004/1‑250 (MQ=255)                                     tCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTATTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATa                                                                                                                                                                                                           >  2:45216/1‑249 (MQ=255)                                       cACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTAGATACGCTTTAAAAAC‑‑AATAAggg                                                                                                                                                                                                       >  1:606839/1‑251 (MQ=255)                                        aCTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGc                                                                                                                                                                                                      >  1:244588/1‑251 (MQ=255)                                        aCTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGc                                                                                                                                                                                                      <  1:410628/251‑1 (MQ=255)                                         cgacagccTTATACCAGGGCATTGATGTCGGCAGTCTCCATCCCTGAGCCGGATCTGGAGAAGAACAAAACCATCCAGTTACCGGAGGGGGAATTCCCGGCGCCGATCAACCCGCCTTCCGGTTTTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCt                                                                                                                                                                                                     <  2:104420/245‑1 (MQ=255)                                            aaTCCTTACACCAGGTCATTGATTGCGGCAGCACCTATCCCTGATCCGGATATGGAGAAGGACAAAACCATCCAGTTACTTGAAGGGGAATTACCGGCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTGCCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACCCGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGAc                                                                                                                                                                                                  <  2:185463/250‑1 (MQ=255)                                               ccTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCATGTTCTGGAGGGGCGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGt                                                                                                                                                                                               >  2:60411/1‑251 (MQ=255)                                               ccTTACACAAGGGCATGGATGGCGGCAGTCCCCATCCCTGATCCGGATCTGGAGAAGAACAAAACCATCCATTTACTGGAAGGGGAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTGTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGt                                                                                                                                                                                               <  2:637967/251‑1 (MQ=255)                                                  tACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGtt                                                                                                                                                                                              <  1:45216/249‑1 (MQ=255)                                                   acacCAGTGCATTGAGGTCGGCAGTCCCCATCCCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTGCTGGAAGGGGAATTACCGGCGCCGATCACCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGt                                                                                                                                                                                            <  1:379744/250‑1 (MQ=255)                                                      ccAGGGCATAGATGTCGGAAGTCCCCAGTCCTGATCCGGATCTGGAGAAGAACAAACCCATCCATTTACTGGAAGGGGTATTACCGTCGCCGACCAACCCGCCTTCCGGTTGTGTTTTCCGTTCCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACTTCCTGTTCGGGAGGGGAGTTTCAAACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAg                                                                                                                                                                                         <  2:559638/250‑1 (MQ=255)                                                           gCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCg                                                                                                                                                                                                                           >  1:374528/1‑213 (MQ=255)                                                                  tGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTCCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCTGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGc                                                                                                                                                                            >  1:90365/1‑251 (MQ=255)                                                                            ccccATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGCAAGTCGATCCGCGTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTCAAAGCGat                                                                                                                                                                  >  2:560151/1‑251 (MQ=255)                                                                            ccccATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCATGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGat                                                                                                                                                                  <  1:60411/251‑1 (MQ=255)                                                                                       aTCCGGATCTGGAGAAGAACAAACCCAGCCAGTGACTGGAAGGGGAATTCCCGTCGCCGATCAACCCGCCTCCCGGTGTTTTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCt                                                                                                                                                                           <  2:85333/231‑1 (MQ=255)                                                                                       aTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCt                                                                                                                                                                           >  1:85333/1‑231 (MQ=255)                                                                                       aTCCGGATCAGGAGAAGAAAAAACCCACCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCTGCCCTTTTTCACGCTAAAAGCGATTATTTATTccc                                                                                                                                                       <  1:547004/251‑1 (MQ=255)                                                                                             aTCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                                                                    >  1:675774/1‑248 (MQ=255)                                                                                                tGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCACCCCTCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGTTCCAGAGTG‑CGCCAAAACACTTCCTGTTCTGGAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGTGCTGACATTTg                                                                                                                                                                                             <  1:386492/204‑1 (MQ=255)                                                                                                tGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTg                                                                                                                                                                                             <  1:386472/204‑1 (MQ=255)                                                                                                tGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTg                                                                                                                                                                                             >  2:386472/1‑204 (MQ=255)                                                                                                 ggAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTGCCGTCGCCGATCAACCCGCCTGCCGGTTGTGTTTTCCGTACCCGTGGCCCGATTGCCGGTCCAGAGGG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCGGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAga                                                                                                                                                 <  1:357962/247‑1 (MQ=255)                                                                                                  gagaAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatgat                                                                                                                                             >  2:321411/1‑250 (MQ=255)                                                                                                       gaaCAAAACCATCCAGTTACTGGAAGGGGAATGACCGACGCCGATCAACCCGCCTTTCGGTTGTGTTTTCCGTACCCTTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGGCCTGTTCTGGAGGGGAGTTTCAGACACGCCGGTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCa                                                                                                                                       <  1:425728/251‑1 (MQ=255)                                                                                                        aaCAAACCCAGCCAGTTCCTGGAAGGGGAATGCCCGTCGCCGATCAACCCGCTTTCCTGTTGTGTGTTCCGTCCCCGTTGCCCGTTTGCCGGTCCAGTGTG‑CGCCAAAACACGTCCTGTTTTGGAGGGGATTTTCAAACACGCCGGTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                                                                                                                      <  1:650495/251‑1 (MQ=255)                                                                                                        aaCAAAACCAGCCTGTTACTGGAAGGGAAATTCCCGTCCCCGATCAACCCGCCTACCGGTTTTGTTTGCCGTACCCGTTGCCCGGTTGCCGGTCCAGAGTG‑CTCCAAAACACGTCCTGTTCTGGAGGGGAGTTTAAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAATGGCTGACAGTTGTCATCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCag                                                                                                                                      <  2:415803/251‑2 (MQ=255)                                                                                                          aaaaaCCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCGTTCCGGTTGTGTTTTCCTTACCCGTTCCCCGGTTGCCGGTCCAGAGGG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                                                                    <  1:164300/250‑1 (MQ=255)                                                                                                                cattctgtacgtGGAAGGGGAATTACCGGCGCCGATCAACCCGTCATCCGTTTTTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCAAAAACACGTCCTGTTCTGGAGGAGAGTTTCAGACTCGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTGCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGATtgtg                                                                                                                              <  2:249092/240‑1 (MQ=255)                                                                                                                cATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCATCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGTTCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                                                                                                              <  1:702156/251‑1 (MQ=255)                                                                                                                cATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCAGGTTGTGTTTTCAGTACCCGTTGCCCGATTGCCAGTCCAAAGCG‑CGCCAAAACACGTCCTGCTCTGGAGGGGAGTTTCAGCCACGCCGATTCTTGCCTGAAAGTCGATCCGCTATAAAAACCAAATAAG‑‑CTGACAGATGTCAGCCCTTTTTCACGATAAAAGCGATTATTTATTCCCGCCAGCTGataggtccaagcttgtg                                                                                                                              >  2:591029/1‑237 (MQ=255)                                                                                                                             gAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGTTTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATa                                                                                                                                                                                                           <  1:122612/161‑1 (MQ=255)                                                                                                                             gAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATa                                                                                                                                                                                                           >  2:122612/1‑161 (MQ=255)                                                                                                                                     aTTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAg                                                                                                         <  1:663877/251‑1 (MQ=255)                                                                                                                                           gTCGCCGATCAACCCGCCATCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTTTTGCCTGAAAGTCGATCCGATTTAAAAAC‑‑ACTACGGGCTGACAGTTGTCATCCCtt                                                                                                                                                                                    >  2:155898/1‑170 (MQ=255)                                                                                                                                             cGCCGATCAACCCGCCTTCCGGGTGTGTTTTCCTTCCCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATGTAGTCCCGCCAGATGATATGGCAAAGTGTGTGATCTTTTTCGCGGCATAACAGAATGCggg                                                                                                 <  2:255779/251‑1 (MQ=255)                                                                                                                                              cccaaccacccctcctgacGGTTGTGTTTTCCGTACCCTTTGCCCGATTGCCGGTCCAGAGTG‑GTCCAAATCACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGc                                                                                                <  2:539335/233‑1 (MQ=255)                                                                                                                                               ccGATCACCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCa                                                                                               <  1:657200/251‑1 (MQ=255)                                                                                                                                                cGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCa                                                                                               >  1:501220/1‑250 (MQ=255)                                                                                                                                                                      tgtTTTCCGTACACGTTACCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGATGACCGTTGTCAGCCCTTTTTCAAGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGCTCTTTTTCGCGGCATAACAGGATGCGGGCAAAAACATCGTTGATTTCACCATc                                                                        >  2:334500/1‑251 (MQ=255)                                                                                                                                                                        ttttCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCttttt                                                                                                                                                                                 >  2:111551/1‑144 (MQ=255)                                                                                                                                                                        ttttCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCttttt                                                                                                                                                                                 <  1:111551/144‑1 (MQ=255)                                                                                                                                                                                        cccGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTAACCATATTCACTGTCCGCCAGAcc                                                      >  2:115186/1‑251 (MQ=255)                                                                                                                                                                                         ccGATTGCCGGGCCAGA‑TGTCGCCAAAACACGTCCTGGTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCTTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCATCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCa                                                     <  2:537769/251‑1 (MQ=255)                                                                                                                                                                                               gCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATACCAGAATGCGGGCAAAAACATCGTTGATTTAACCATCTTCACTGTCCGCCAGACCAATcacc                                               >  1:390527/1‑251 (MQ=255)                                                                                                                                                                                                ccGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATcacca                                              <  1:68248/251‑1 (MQ=255)                                                                                                                                                                                                 cggcccAGAGTG‑CGCCAAAACACGTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCTGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAACAAGcaccac                                             <  2:90365/247‑1 (MQ=255)                                                                                                                                                                                                              cgcCAAAACAAGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCACACCAATCACCACTTCGGCaaata                                  >  2:322161/1‑248 (MQ=255)                                                                                                                                                                                                                  aaaaCACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTcc                             >  1:430456/1‑251 (MQ=255)                                                                                                                                                                                                                                  tgtaGGGGAGTTTCAGACACGACGTTTCTTGCCTGAAAGTCGATGCGCTTTATAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCACGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCAGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACCGTACGCCAGGCCCATCACCACTTCGGCCAAAAAGACAGGGTTCCAATCGAAAt             >  2:21966/4‑251 (MQ=255)                                                                                                                                                                                                                                             ttCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTAGTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTc   <  1:564795/250‑1 (MQ=255)                                                                                                                                                                                                                                              ttaGACACGCCGTTTCTTGCCTTAAAGTCGATCCGCTTTAAAATC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTGCACGCTAAAAGCGATTATTTATTCCCGCCAGAGGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCAGCCTCACTGTCCGCCATTCCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTcc  <  1:95933/248‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTG‑CGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAC‑‑AATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCC  >  NC_000913/1306492‑1306977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑