Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,594	A→C	F63V (TTT→GTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,594	0	A	C	95.8%	126.5 / ‑3.4	48	F63V (TTT→GTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (15/31);  total (16/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

GGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑AAAATCC‑ATCAAAAAACCAGGCTTGAGTATAGCCTGGTTT  >  NC_000913/65349‑65824                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               gggAGGCGGTTTCAGACGGGCATTATAGATAGCACCATTGTGCATACCACCCCCGACCCCCACCGGTGAGGTGATAAACCGCTCCATCAGTGAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCTCCTGCACGCAGGCGCTTTTCGTAATTCAGCAATGGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCTGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCtt                                                                                                                                                                                                                                           <  2:32553/251‑1 (MQ=255)    aTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGG‑AAAA‑TCCTTTAACGCCCGCGG‑TGTCAAGCGAACGCCTTGtt                                                                                                                                                                                                                                        >  1:701041/1‑251 (MQ=255)             cAGACGGGCAGTAACGATAGCGCCATGTTGCATATCACCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCa                                                                                                                                                                                                                               <  2:123459/251‑1 (MQ=255)                        tAACGCTAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGCGATACACCGATCCATCAGATCGCGATCTGGCGGGCGCACATCGGACTCGTAGACGGTTACGCCACCTTCAAGCAGGCGCTTTTCGTAATTCCACCATTGGCCAGGGGCGCGAACGGAAAGGCCATACAACGGCCGGCGGGGTAAAC‑TCCTTAAACGCCAGGGG‑TG‑GAGGCGAAAGCCttatttcacacgccaaaaagcac                                                                                                                                                                                                                      >  2:668198/1‑228 (MQ=255)                             aTAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATAAACCCGCTGGCGGTG‑GAAA‑TCCTTTAAAGCAAAAGGATG‑CAAGCGAACGCCTTGTTAACCCTGCAAAataatgtgagctgg                                                                                                                                                                                                               >  1:551647/1‑240 (MQ=255)                                 cgccttggcacatcacctccTCGACCCACCCCGGTTAAGTGATAACCCGCTCCATCAGATAGCGTTCTGGCGGGCTCACATCGGCCTCTTAGACGGTCACGCCTCCTTCACGCACGCTCATTTCGTAATTCATCAATTGGCTATGGGGGCGACAGTAAAGGCCATCCACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGGCAGGCGAACTCCTTGTTCACCCTGTAAAATATGCTGTGCGCggtg                                                                                                                                                                                                            <  2:498561/232‑3 (MQ=255)                                          gCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATcg                                                                                                                                                                                                  <  1:531674/251‑1 (MQ=255)                                            atatCCCCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCTCCTTCACGCAGGCGCTGTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATcggc                                                                                                                                                                                                <  2:575412/251‑1 (MQ=255)                                            atagcACCCCCGTCCCACACCGGTGGGGTGATAAACCGTGCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCGGAGCGCGGTGAACCTGATcggc                                                                                                                                                                                                <  2:8225/247‑1 (MQ=255)                                                          cctaacCGTTGAGGTGATAAACCTCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCTAACGCCGTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACggca                                                                                                                                                                                 <  2:577322/247‑4 (MQ=255)                                                                  gtgaggtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGAAAAAAC‑CCACGGGctct                                                                                                                                                                         >  1:307017/1‑251 (MQ=255)                                                                    gaggtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCtt                                                                                                                                                                        <  1:64270/250‑1 (MQ=255)                                                                       gtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGAAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACCCCAGCGG‑TGTCAGGCGAACGACTTGTTCACCCTGCAAAATATGCTTAGGGCGGGGAACCTGACCGGCGGG‑AATAAAcaacccaggcgtttgtcg                                                                                                                                                                    >  1:403798/1‑234 (MQ=255)                                                                             aaCCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCtt                                                                                                                                                                                                                                           <  2:108316/175‑1 (MQ=255)                                                                             aaCCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑GGTCAGGCGAACGCCtt                                                                                                                                                                                                                                           >  1:108316/1‑175 (MQ=255)                                                                                     cATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCTCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGTCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCt                                                                                                                                                      <  1:80995/251‑1 (MQ=255)                                                                                     cATCAGATAGCGTCCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCGTCACGCAGGCGCTTTTCGTACTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCTGTGCAAGCGTAACCt                                                                                                                                                      <  2:522529/251‑1 (MQ=255)                                                                                      aTCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTg                                                                                                                                                     <  1:154289/251‑1 (MQ=255)                                                                                         aGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCaa                                                                                                                                                  <  2:701041/251‑1 (MQ=255)                                                                                                                  cGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCACGCGTAACCTGCAACGGCCCGTTGTCCGTCGccagccag                                                                                                                         <  1:186361/251‑1 (MQ=255)                                                                                                                      cTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGGCTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGGTGTCCGTCGCCAGCCAGAAgg                                                                                                                     >  1:174477/1‑251 (MQ=255)                                                                                                                        cGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑GCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGga                                                                                                                    <  2:157707/250‑1 (MQ=255)                                                                                                                               ggTAACGCCACCTTCACGCAGGCGTTTATCGTAATTCATCAAGTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCCCCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTg                                                                                                           <  2:488484/251‑1 (MQ=255)                                                                                                                                    cGCCCCCTTCACGCAGGCTCTTTTCTCAATTCATCAATTGGCGACGGGAGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCt                                                                                                     <  2:174477/251‑1 (MQ=255)                                                                                                                                       cACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGc                                                                                                  <  1:139121/251‑1 (MQ=255)                                                                                                                                                 cAGGCGCTCTTCGTATTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGGGGAAACTCCAACGGCCCGTTGTCCGTCGCCCGCCCGAAGGAGA‑ATTCTGTC‑CCCTGCGGGGGGT‑ccc                                                                                       >  1:379234/1‑251 (MQ=255)                                                                                                                                                  aGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCg                                                                                      <  1:166601/251‑1 (MQ=255)                                                                                                                                                      gctgttCGTAACTCAACAATTGGGTGTGTGCGGGACAGTAAAGGCCCTACACCGGCTGGCGGGT‑AAAA‑GCCTTTAACGCCAGCGG‑TGTCAGGCTAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGA‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTTCGCCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CTGTGCGTGGTTT‑CCCGCCAg                                                                                  <  2:379666/247‑1 (MQ=255)                                                                                                                                                         tttCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAgtg                                                                                <  1:261414/250‑1 (MQ=255)                                                                                                                                                           tCGTAATTCACCAATTGGCGATGGGCGCGACAGTAAAGGCCATACCCCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCCCCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGGC‑CCTTGCGGGTTGT‑CCCGCCAGTG‑Tc                                                                             <  1:194059/250‑1 (MQ=255)                                                                                                                                                               aaTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAAAGCCTTGTTCCCCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGCCTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGtt                                                                       <  1:206250/251‑1 (MQ=255)                                                                                                                                                                 ttCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCCCGGACCCTTGCGGTGCCAACGGAACCTGCAACGGCCCCTTGTCCGTCCCCAGCCCGAAGGAGA‑CTTCTGTC‑CCTTTCGGGGGGG‑CCCGcccggggcggggta                                                                        >  1:677838/1‑238 (MQ=255)                                                                                                                                                                 ttCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AAAAAACGCCCCGGACTCTTGCGGTGCAAACGTAACCTGCAACGGCCCGGTGTCCGTCGCCCGCCCGAAGG‑GAGCCTCTGTC‑CCCTGCGGGG‑GTGCCCGCCCGTG‑TC‑ggggtt                                                                      >  1:276447/1‑247 (MQ=255)                                                                                                                                                                       aaTTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑GATAAACCCCCCGGACTCTTTCGGGGCCAGCGTAACCCGCAACGGCCCCGTGTCCGTCGCCCGCCCCAAGGAGA‑CCTCTGTC‑CCTTTCGGGGGGT‑cccccccgagtccgggttagaaga                                                                  >  1:688177/1‑229 (MQ=255)                                                                                                                                                                               aTGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑GATAAAAGCCCCGGACTCTTGCGGTGCAAGCGTAAACTGCAACCGCCCGTTGTCCGTCGCCCGCCACAAGGAGA‑ATTCTGTC‑CCTTGCGGGGGGT‑CCCGCCCGTGGCC‑GGGTTA‑GATaaaaacatgcc                                                       >  1:293315/1‑244 (MQ=255)                                                                                                                                                                                      ggACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCCCCCTGCAAAATATGCTGAGCGCGGGTACCCTGATCGGCGGG‑AATAAACGCCACGGACTTTTGCGGGGCAAGCGTAACCTGCAACGCCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGCC‑CCTTGCGGGTTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCAc                                                <  1:264223/250‑1 (MQ=255)                                                                                                                                                                                          aGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTg                                            <  1:115244/251‑1 (MQ=255)                                                                                                                                                                                             aagggccctacaccgtcgtgCGGGG‑AAAA‑CCCTTTACCGCCAGGGG‑TGTCAGTCGAACGCCTTGTTCACCCTGCAAAATAGGCGGAGCGCGGGGACCCTGATCGGCGGA‑AATAACCGCCACGGACTCTTGCGGTGCAAGCTTACCCGTCACCGGCCCTGTGTCCTTCGCCAGCCAGAAGGAGT‑CTTCTTCC‑CCTTGCGGGGTCC‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCTACGCTgta                                          <  2:701050/231‑3 (MQ=255)                                                                                                                                                                                               aGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCCGCGG‑TGTCAGGCGACCGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑ACAAAACCACACGGGCTCTTAGGGTGCGAACGTAACACGCAACGGGCCGCTTTCCCTCGCCAGCCCGAAGGAGA‑CTTCTGTA‑CCTTGCGGGGTGT‑CCCGACAGGG‑TC‑GGGGGAAAATAAAAACTGCCCGCGCCAccccgtcatt                                       >  1:709255/1‑242 (MQ=255)                                                                                                                                                                                                     aacacCGGCTGGCG‑TG‑AAAAACCCTTTAACGCCAGCTG‑TGTCAGGCGATCGCCTTGTTCACCCTGAAAAATAGCCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCGTAAACGTCCCGGTGGCCGTCGCCAGCCATAAGGAGA‑CTGCTGTC‑CCTTGCGGTGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAGCCTGCCTGCGCCACGCTG‑TAATTCC‑ATCa                               <  2:403798/250‑1 (MQ=255)                                                                                                                                                                                                      cccccgggggggggTG‑AAAA‑TCCTTAAACGCCAGCGG‑GTTCAGGCGAACCCCTTGTTCCCCCTGAAAAATATGCTGGGCGGGGGGAACCTGACCGGGGGG‑AATAACCGCCAGGGACTCTTGCGGTGAAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGC‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑TAATTCC‑ATCaa                              <  2:138616/239‑1 (MQ=255)                                                                                                                                                                                                      acacCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑TAATTCC‑ATCa                               <  1:533494/250‑1 (MQ=255)                                                                                                                                                                                                      acacCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑TAATTCC‑ATCa                               <  1:484242/250‑1 (MQ=255)                                                                                                                                                                                                             cTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACACCCCGGACTCTTTCGGTGCCAACGTAACCTGCAACGGCCCGTTGTCCCTCCCCCGCCAGAAAGAGA‑CTTTTTTCTCCTT‑CGGGGGGT‑CCCCCCCGGG‑TCCGGGTTAAATTAAAACCT‑CCTGCCCCAccccttaaattccccaaaaaaac                          >  2:680083/1‑228 (MQ=255)                                                                                                                                                                                                                  ggTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACAGCCCGTTGTCCGTCGCCAGCAAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGGTAAGATAAAAACCGCCTGCGCCCCGCTG‑TAATTCCCTTCAAAAAAC‑AGGCtt                  >  1:380146/1‑251 (MQ=255)                                                                                                                                                                                                                                 tAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGAAAACGTAACCCGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCCGTG‑TC‑GGGGTAAAATAAAACCTGCCTGCGCCACGCGGTAATTTCC‑A‑CAAAAAACCAGGCTTTATTATAGcccgg     >  1:326304/1‑248 (MQ=255)                                                                                                                                                                                                                                 tAACGCCAGCGG‑TGTCAGGCGAACGCCTTGTTAACCCTGCAAAATATGCGGAGCGCGGGGAACCTGACCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑TAATTCC‑ATCAAAAAACCAGGCTTGATTATAGCCTg      <  1:483051/250‑1 (MQ=255)                                                                                                                                                                                                                                       cAGCGG‑TGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGACCGGCGGG‑AATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑TAATTCC‑ATCAAAAAACCAGGCTTGATTATAGCCTGGttt  <  1:655918/248‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG‑AAAA‑TCCTTTAACGCCAGCGG‑TGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGA‑CTTCTGTC‑CCTTGCGGGGTGT‑CCCGCCAGTG‑TC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑AAAATCC‑ATCAAAAAACCAGGCTTGAGTATAGCCTGGTTT  >  NC_000913/65349‑65824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑