Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	136.8 / NA	46	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCG  >  NC_000913/1169590‑1170076                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ggCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGCCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCAAGAC‑GAATCGCATGATGGCCCATTGGGTTAtccggtcc                                                                                                                                                                                                                                              >  2:191743/1‑251 (MQ=255)     gATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGcc                                                                                                                                                                                                                                          <  1:120282/251‑1 (MQ=255)              cAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTAcc                                                                                                                                                                                                                                 >  1:675415/1‑251 (MQ=255)                  cAGACGCGCACCGCTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTATAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGACAGCACTTACCCGCATGCCAATGCCGAAATCGGCGTTAGAACCATGAAGCAAATAAACGACGCCCTAGACCGCCAGACAGAA‑CGCATGATGGCCCATTGGATTATCCGGTCCCGCCGGCAATACCGccg                                                                                                                                                                                                                             >  2:605690/1‑249 (MQ=255)                                ttGGTGCGGCAGGGACTGTGATCGGAGTGTTGATGGTATTCCCTTTGGTGCTGGGGGTGACCTGGCTAAAGAGTGTTTTGATACCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAAGGCCGAAATCGGGGTTCGATCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTcc                                                                                                                                                                                                                <  2:319693/250‑1 (MQ=255)                                      cGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATTCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATTAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCTAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCt                                                                                                                                                                                                         >  1:271562/1‑251 (MQ=255)                                         cAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCttt                                                                                                                                                                                                       >  1:335645/1‑250 (MQ=255)                                                 tttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCACATTGGGTTATCCGGTCCAGCCGGCACTCCCGCAGGCAACTCAATTACCTGTGCTTTATAAcaggg                                                                                                                                                                                              >  2:86208/1‑247 (MQ=255)                                                      cGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCggg                                                                                                                                                                                         >  2:577055/1‑250 (MQ=255)                                                       ggAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGCAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCCGCCGGCACTACCGCAGGCAACTCAATTCACTGTGCTTTATACCGGGCGCGGAt                                                                                                                                                                                        >  2:285760/1‑251 (MQ=255)                                                        gagggtTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCCAAAGAGTGTTTTGATAGCGGCACCCCGCAGACTAATACAGCCAGCACTTACCCGCTTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCATAC‑GAAGCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATg                                                                                                                                                                                       <  1:55041/247‑1 (MQ=255)                                                                   atatTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgg                                                                                                                                                                            >  1:110277/1‑251 (MQ=255)                                                                    ttttcctgttGGTGCTTGTGGTGCACTGGCTAAAGAGTGTTTTAATAACGTCATCCCGCAGCCGAATACAGCCAGAGCTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAACTAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCAATGGGTTATCAGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCTGATGTTTGCCGTTgg                                                                                                                                                                            <  2:258161/242‑1 (MQ=255)                                                                         aCTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAAGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTct                                                                                                                                                                       >  2:631103/1‑249 (MQ=255)                                                                                     ggggTGACCTGGCTAAAGGTTGTTTTGATATCGCCATCCCGCAGACGAATACAGCCAAAAATTACCCGCATGCAAAGGCCGAAATCGGCGTGCGTACCATAAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAAGTCCCTGTGCTTTATAACGGGCGCGGATGTTGGCCGTTGGCGTCCAGGTTGGGTTTg                                                                                                                                                           <  2:675415/250‑1 (MQ=255)                                                                                      gggTGACCTGGTCAAAGAGTGTTTTGATATCGTCACCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCa                                                                                                                                                         <  2:668880/251‑1 (MQ=255)                                                                                         gtaCCTGGCTAAAGATTGTTTTGATATCGTCATCCCGCGAAGGAATACAGCCAGAACTTACCCGCATGCCAAGGCCGAAAGCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACGACCGCAGGCAACTCAAGTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGTTTGGGTTTGCACGt                                                                                                                                                      <  2:637782/249‑1 (MQ=255)                                                                                          gACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATCCAGCCAGAACTTACACGCATGCCAATGCAGAAATCGGCGTTCGTACCATGAAGCAAATAACCACCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTAGCTTATCCGGTCCCCCCGGCACTACCGCAGGCACCTCACTTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCTTCCAGGTTGGGTTTGCAGGtt                                                                                                                                                     >  1:439795/1‑251 (MQ=255)                                                                                                 cTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTg                                                                                                                                               >  1:91945/1‑250 (MQ=255)                                                                                                                atatCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCAt                                                                                                                               <  1:16793/251‑1 (MQ=255)                                                                                                                       cATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgt                                                                                                                        <  1:594190/251‑1 (MQ=255)                                                                                                                                 aCGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCCACTCAATTCCCTGAGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGGCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGGGGTCACCATTGTCGGTGTCAGCgtgt                                                                                                              >  2:525705/1‑251 (MQ=255)                                                                                                                                    aatcccgCCAGAACTTACCCGCAGGACAATGCCGAAACCGGCTTTCTTACCATGAAGAAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTc                                                                                                             <  2:483993/243‑1 (MQ=255)                                                                                                                                            cAGAACTTACCCGCATACCAATGCCGTAATCGGCGTTCGTACCCTGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCCTTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCCATTCCCTGTGCTTTATCACGGGCGCGGACGTTTGCCGTTGGCGTCCAGGTTGGGTTTTCCCGTTTGTCTGAAACGGTGTTCACCATTGTCGGTGTCAGCGTGTCATCACCTAAc                                                                                                   >  1:211830/1‑251 (MQ=255)                                                                                                                                            cAGAACTTACCCGCAGGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAAc                                                                                                   <  1:613638/251‑1 (MQ=255)                                                                                                                                                        gCATGCCAATGCCGAAATCGGCGTTCGTTCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGAGGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCt                                                                                       <  1:95363/251‑1 (MQ=255)                                                                                                                                                         catgcaaatgtggAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGTATGATTGCCCATGGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTCACTGACCAATACCTa                                                                                      <  2:494509/239‑1 (MQ=255)                                                                                                                                                              cAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTgg                                                                                  >  1:690755/1‑250 (MQ=255)                                                                                                                                                               aaTGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                                                                                                                   >  1:363808/1‑120 (MQ=255)                                                                                                                                                               aaTGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                                                                                                                   <  2:363808/120‑1 (MQ=255)                                                                                                                                                                    cGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGAATGATGGACCAATGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTATGCCGTTGGCGTCCTGGATGGGTTTGCACGCTTGTCGGAAACGGGGGTCACCATTGACGGTGTCAGCGTGTCAACCCCAAACTGACCAAtccataatagataaa                                                                             >  2:388317/1‑235 (MQ=255)                                                                                                                                                                       aaTCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGtt                                                                        <  1:508738/251‑1 (MQ=255)                                                                                                                                                                        aTCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCCGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTAtttagagcgccttt                                                                        >  1:416001/1‑238 (MQ=255)                                                                                                                                                                        aTCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTa                                                                       <  1:529526/251‑1 (MQ=255)                                                                                                                                                                                    aCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTa                                                            <  1:18449/250‑1 (MQ=255)                                                                                                                                                                                      cATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCAATGGGTTATCCGGTCCAGCCGGCACTACCGCATGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGACCAGGTTGGGTTTGCACGTTTGTCTGAAACGGGGGTAACCCTTGTCGGAGTCAGCGTGTCACCACCTAACTGACCAATACCAATTGGATACACGATTACCGAATTTTTAccc                                                         >  2:430033/1‑251 (MQ=255)                                                                                                                                                                                          aaGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCcggcg                                                     <  1:679627/251‑1 (MQ=255)                                                                                                                                                                                                aaaaaCGCCGCCAGACGCCGCCAGAC‑GAATCGCATGATGGCCCAGTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACCCGGTTACCGAATTTTTACCCGGCGGgtagt                                               <  2:270181/249‑1 (MQ=255)                                                                                                                                                                                                  acaCGCCGCCATAGGCCGCCAGAG‑GTATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCATCTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTaa                                             <  2:667234/249‑1 (MQ=255)                                                                                                                                                                                                      gccgccATAGGCCGCCGGAC‑GAATCGCATGATGGCTCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATaga                                         <  2:411931/251‑1 (MQ=255)                                                                                                                                                                                                                 ccgccAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTcc                              <  1:134541/251‑1 (MQ=255)                                                                                                                                                                                                                  ccgcacgacaaatctcattttgGCCTATTGGGTTTTCCGTTCCATCCGGCACTACCGCATGCAACTCAACTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGTTGGTCACCATTGTCGGTGTCAGCGTGTCACCCCCTAACTGACCAATTCCTATTGGATACACGGTTACCGAATTTTTGCCCGGCGTGTAGTAATAGAGACGCAGCTcc                              <  2:64984/231‑1 (MQ=255)                                                                                                                                                                                                                         c‑GAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCCCCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGtt                      <  1:421317/251‑1 (MQ=255)                                                                                                                                                                                                                           gAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTCGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAATCGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTg                     <  1:605690/251‑1 (MQ=255)                                                                                                                                                                                                                            aaTCGCATGATGGCCACTTGGGTTTTCCGGTCCAGCCGGCACTACCGTAGTCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCTCCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTGACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGa                    <  2:3156/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCCCATTGGTTTATCCGGTCCAGCCGGCACTACCGCAGGCACCTCAATTCCCTGTGCTTTATCACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGTTTTGCACGTTTGTCTGAAACGGTGGTCACCCTTGTCGGTGTCAGCGTGTCTCAACCTAACTGACCAATACCTATTTGTTACACGGTTACCGAATTTTTAACCGGCGGGTCGTAATAGAAACGCAGATCCGCAATGTTTGTCACAATGCCtt        >  2:376425/1‑251 (MQ=255)                                                                                                                                                                                                                                               gggTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTcgcgcg  <  1:77246/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAC‑GAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCG  >  NC_000913/1169590‑1170076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑