Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,040,126 G→T L277L (CTG→CTT)  appB → cytochrome bd‑II subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,040,1260GT95.2% 181.4 / ‑3.6 63L277L (CTG→CTT) appBcytochrome bd‑II subunit 2
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (25/35);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCT  >  NC_000913/1039881‑1040367
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
aTTCATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGGGATGCTGTGCTTTTTGCTGGCGGGGTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGt                                                                                                                                                                                                                                               <  1:688799/251‑1 (MQ=255)
    aTCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGGATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTTGGGCCTGGGTGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATg                                                                                                                                                                                                                                           >  1:313163/1‑251 (MQ=255)
                aGCTGGCGACCCAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTTGCTGTGGGGCGGGTTTGATGGCTTTTTACTGTTCTCCCCGG‑ATGCTAAAGGGCCTTTCACACCGTTTATGAAACTGGTGGCA‑AGGCTTCCTGGGGCATGGGTGAATAATTTTTTCGCGGCGCCCGTTTTGGGGGTCTTCCCGCCTCTGGGGTTCTTTTGCCCATTGCGTACGGGGGTGGCGAtttttccg                                                                                                                                                                                                                               >  2:186771/1‑246 (MQ=255)
                   tGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCCATCCGTTAATGAAACTGGGGGCAAGT‑CTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTTTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTggg                                                                                                                                                                                                                            >  1:334329/1‑250 (MQ=255)
                   tGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGGATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GGGCTACCTGGTGCCTGGATGGATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTAAGGTGATGGCGATTTATCGTggg                                                                                                                                                                                                                            >  1:167769/1‑250 (MQ=255)
                    ggCGACCAAACGCGCTGCTCTGTTGGTGATGCTGTGCTTTTTGCTTGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCGTTGGACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTc                                                                                                                                                                                                                           <  1:181299/251‑1 (MQ=255)
                    ggCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGGGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTc                                                                                                                                                                                                                           <  2:182110/251‑1 (MQ=255)
                           aaaCGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGAATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAACTTTCCGCTGCTGGGATTCTTCTTGCCATTGCTTACGGTGATGGCGATTTATCGTGGTCTCCCggg                                                                                                                                                                                                                    >  1:593510/1‑251 (MQ=255)
                           aaaCGAGCTGCACTGTTGGTGTTGCTGAGCTTTTTGCTGGCCGGTTTCTGGTTTTGGGTCGGTATTGATGGCTTTGTACTGCTCTCCCAGG‑ATGCTAACGGTCCTTCCAATCCTTTAATGAAAATGGTGGCA‑GTGCTTCCTGGTGCCTGGATGAATAAGTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCGGCCCATTGTTTACGGTGATGGCGATTTATCGTGTTCGCCCggg                                                                                                                                                                                                                    <  2:479732/251‑1 (MQ=255)
                            aaCGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑t                                                                                                                                                                                                                  <  1:330468/251‑1 (MQ=255)
                             aCGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGGAGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAAAGGTCCTTCCAATCCGTTAATGAAACTGGGGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCGGCTGGGGTTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑tt                                                                                                                                                                                                                 >  1:339822/1‑251 (MQ=255)
                              cgcgCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTg                                                                                                                                                                                                                <  1:676469/251‑1 (MQ=255)
                                      aCTGTTGGTGGTGTTGTGCTTTTTGCTGGCGGGTTACTTGTTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTTCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCTATTGCTTACGGTGATGGCGATTTATCGTGGTCGACCGGG‑TTGGGGAtttt                                                                                                                                                                                                        <  2:69709/251‑1 (MQ=255)
                                      aCTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGATGGGGGGCGGGATTGGTGGCTTTTTTCTACTCGCCCAGGGAT‑CTAACGGGCCTTCCCAACCGTTAATGGAACTGTGGGCC‑GGGGTTCCCGGTGCCTGGGAGAATAATTTTTTTGGGGCGCCCGTTTTGTGGGTCCTCCCCGAGCTGGGGTTTTTCTGCCCCTTGCTTTAGGGGAGGGCGATTTATCGTGGTCACCCGGGGTTGGGGtttt                                                                                                                                                                                                         >  2:617601/1‑251 (MQ=255)
                                         gTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTg                                                                                                                                                                                                      <  1:396499/250‑1 (MQ=255)
                                               gATGCTTTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCTGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGGTCCTGGATGAATAATTTTGTCGAGTCGCCCGTTGTGTGGATCGTCCCGCGGCTGGGATTCTTCTGCCCAGTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGcat                                                                                                                                                                                               <  1:419121/251‑1 (MQ=255)
                                                          ttttGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCa                                                                                                                                                                                     <  1:223731/250‑1 (MQ=255)
                                                                   cGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCACTTTGGCGTg                                                                                                                                                                           >  1:124838/1‑251 (MQ=255)
                                                                      gTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGAtt                                                                                                                                                                        >  1:602909/1‑251 (MQ=255)
                                                                        tACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGAttt                                                                                                                                                                       <  1:22521/250‑1 (MQ=255)
                                                                          cTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTc                                                                                                                                                                     >  1:261274/1‑250 (MQ=255)
                                                                                gtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACggcag                                                                                                                                                              <  1:74426/251‑1 (MQ=255)
                                                                                gtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACggc                                                                                                                                                                <  1:20005/249‑1 (MQ=255)
                                                                                 tgGGTCGGTATTGCTGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTCCGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGGTGGCATCATTGATGCCATTTGGCGTGATTTTCACggcagg                                                                                                                                                             >  2:664292/1‑251 (MQ=255)
                                                                                  gagTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGACTTATCGTGGTCGCCCGGG‑TTGGGGCTTTTTGTTGGCATCATTGATGCATTTTGGCGTGATTTTGACggaag                                                                                                                                                              >  2:8108/3‑246 (MQ=255)
                                                                                       gggtttGATGGCTTTGGACTGCTCGCCCAGG‑ATGCTCACGGGCTTTCCAATCCGTTAATGAATCTGGTTGCA‑GTGCTGCCTTGTTCCTGTATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCTATTTATCGTGGTCGCCCGGG‑TTTGGGATTTTGGTTGGCTTCATTGATGCAATTTGTCTTGATTTTCTCGTCAGGCATCa                                                                                                                                                        <  2:416757/246‑1 (MQ=255)
                                                                                       ggTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCAc                                                                                                                                                       <  1:348615/251‑1 (MQ=255)
                                                                                        gTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATTCGTTAATGAAACTGGTGGCA‑GTGCTTCCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTTCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACg                                                                                                                                                      <  1:689491/251‑1 (MQ=255)
                                                                                                         cTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCAt                                                                                                                                     >  1:514586/1‑251 (MQ=255)
                                                                                                                       aTGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTg                                                                                                                        <  1:481413/251‑1 (MQ=255)
                                                                                                                             aCGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCGTAACGCTGTTCCCCTTTGTCATGGCGTCAAGCGTGGGTCCg                                                                                                                  >  2:44753/1‑251 (MQ=255)
                                                                                                                               ggTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGAt                                                                                                                >  1:362429/1‑251 (MQ=255)
                                                                                                                                     tCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCa                                                                                                           <  1:338273/250‑1 (MQ=255)
                                                                                                                                        aaTCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCt                                                                                                       >  1:89076/1‑251 (MQ=255)
                                                                                                                                           ccGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCTACACGCTGTTCCCCTTTGTCATGCCGTCAAGCGGGAGTCCGATCTCCAGCCTGAc                                                                                                    >  1:4325/1‑251 (MQ=255)
                                                                                                                                                 aTGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTtgtg                                                                                              >  1:376050/1‑251 (MQ=255)
                                                                                                                                                        tggtggCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCCTGCCGTAAAGCGTGAGGCCGATCTCCAGCCTGTCGTTGTGGGACAGt                                                                                       >  2:308441/1‑251 (MQ=255)
                                                                                                                                                         gggtgCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCTTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTGTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAg                                                                                        <  2:376050/245‑1 (MQ=255)
                                                                                                                                                                 gTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAg                                                                               <  1:617601/251‑1 (MQ=255)
                                                                                                                                                                      accgtgTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGGGGATCTTCCCGCGTCTGGGATTCTTCTTCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGACCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGTCTGACGTTGTGGGACAGTACTTCCAGTCAg                                                                           <  2:465485/245‑1 (MQ=255)
                                                                                                                                                                       ccaggTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATAATTGATGCAATTTGGCGTGATTTTCCCGGCAGGCCTCACGCTGATCCCCTTTTTCACGCCTTCAAGCGTGATTACCATATCCAGCCCTACGTTGTAGGACATTACTTCCCTTCAGCt                                                                         >  2:491777/4‑251 (MQ=255)
                                                                                                                                                                             gCCTGGATGAATAATTTTTTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCt                                                                   <  2:261274/251‑1 (MQ=255)
                                                                                                                                                                             gCCTGGATGAATAATTTTGGCGAGTCGCCTGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCt                                                                   <  2:271475/251‑1 (MQ=255)
                                                                                                                                                                                tGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGCTTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TCGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTAACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCACGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTAAGCTgccgatg                                                                  >  1:313982/1‑243 (MQ=255)
                                                                                                                                                                                 ggtagtataatTTTTTCGAGTCGCCCGGTTTGTTGATCTTCCCGCTGCTGGGATTTTTCGGCCCCTTGCTTACGGTGATGGCGCTTTATCGTGGTCGCCCGGG‑TTGGGGATGTTTGATGGCATCATTGATGCAAGTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTTCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCTGCCTGACGTTGATGGACAGTACTTCCAGTCAGCTGACGCTgtgc                                                               <  2:705085/245‑4 (MQ=255)
                                                                                                                                                                                 ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGc                                                               <  1:577266/251‑1 (MQ=255)
                                                                                                                                                                                  gATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGtt                                                                                                 <  1:143467/216‑1 (MQ=255)
                                                                                                                                                                                     gAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCAtt                                                            >  1:601683/1‑250 (MQ=255)
                                                                                                                                                                                          atTTTGTCGAGTCGCCCGTCTTGTTGATCTTCTCGCTGCTGGTATTCTTTTGCCTATGGCTTAATGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGTCTGACGTGGTGGGACAGTACTTCCAGACAGCTGACGCTGAGCATTATggtg                                                      <  2:177955/251‑4 (MQ=255)
                                                                                                                                                                                            tttGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTgg                                                     <  1:224177/250‑1 (MQ=255)
                                                                                                                                                                                                       gCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTgg                                         >  1:358369/1‑250 (MQ=255)
                                                                                                                                                                                                        ccctttttGTGGATCTTCCCGCTTCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGTCCGGG‑TTGGGGATTTGTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGat                                        <  2:167769/247‑1 (MQ=255)
                                                                                                                                                                                                        cccGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTGTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGat                                        <  1:61407/251‑1 (MQ=255)
                                                                                                                                                                                                         ccGTTTTGTGGATCCTCCCGCGGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGata                                       <  2:553667/251‑1 (MQ=255)
                                                                                                                                                                                                             tttGTGGATCTTCCCGCTACTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGTTAtttt                                   >  1:359678/1‑251 (MQ=255)
                                                                                                                                                                                                                  ggATCTTTCCGCTGCTGGGATTCTTCTGCCCATTTCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTTGGTATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGccc                              <  1:394284/251‑1 (MQ=255)
                                                                                                                                                                                                                   gATCGTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCa                             <  1:261083/251‑1 (MQ=255)
                                                                                                                                                                                                                       ttCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACa                                                                                         <  1:381654/187‑1 (MQ=255)
                                                                                                                                                                                                                           cGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTGTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTg                     <  1:607282/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gggATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTa                <  1:137997/249‑1 (MQ=255)
                                                                                                                                                                                                                                   ggATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTAGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTacac             >  1:590104/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gATTCTTCGGCCCATTGCTTACGGTTATGGCGATTTATCGTGGTCGCCCGGG‑TTTGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACAct            <  2:602909/251‑1 (MQ=255)
                                                                                                                                                                                                                                              cacATTGCTTACGGTGATGGCGATTTAGCGTGGTCGCCCGGG‑TTGGGGTTTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGTCGTCTAGCGTGAGTCCGATCTCCAGCCTGACGTTGGTGGACAGTCCTTCCAGTCAGATGACGCTGAGCATAATGTTGGGATTCGTGAGTATAGTGTTGCCCATTGTGTTGCTCTACACACTCTGGAGct  >  2:507426/3‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
ATTCATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGG‑ATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCA‑GTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGG‑TTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCT  >  NC_000913/1039881‑1040367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑