Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,256	G→T	intergenic (+44/‑132)	acnB → / → yacL	AcnB/PF06062 family protein YacL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,256	0	G	T	100.0%	169.6 / NA	51	intergenic (+44/‑132)	acnB/yacL	AcnB/PF06062 family protein YacL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/25);  total (26/25)

GCGAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTT  >  NC_000913/134015‑134500                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     gcgAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGAtt                                                                                                                                                                                                                                             >  1:358155/1‑251 (MQ=255)    aaTGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTccc                                                                                                                                                                                                                                          <  1:603028/251‑1 (MQ=255)                gCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCAttttttt                                                                                                                                                                                                                              <  1:352867/251‑1 (MQ=255)                        ggAACTGGCGACTGTTGCGGCGCTGATTGGCAAACTGCCGACGACGGAAGAGTACCAGACCTACGTGGCGCCGGTAGATAAAACAGCCGTTGATACTTACCGTTATCAGAACTTCAAACAACTTTCTCAGTACACCGAGAAATCCGATGGGGGGGTGTTCCAGCCTGCGGGTTAACAAGACAGCGCACGCTCTGCGCCTAATCGACA‑CAATGCCACGTTCATGAGATTCCCGGTCATTTTTTTATTTCTa                                                                                                                                                                                                                       >  2:620787/1‑250 (MQ=255)                                   cggTTGCGGCGCTGAGTGGCAAACTGCCGACGCCGGAAGAGTACCAGACGGACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGGTATCTGAGCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAGGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGGTAATGAGATTCCCGGGCATTTTTTGATTTCGAAACCATCGCCgg                                                                                                                                                                                                           <  2:290716/249‑2 (MQ=255)                                          ggcgCTGATAGGCAAACTGCCGACGCCGGAAGAGAAACAGAACTACGTGGCGCAGATAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAAGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTATATTTCACAACCATCGACGTTCAGCTg                                                                                                                                                                                                    >  2:470588/1‑251 (MQ=255)                                            cgcTGCTTGGCAAACTGCCGACGCCGGCAGAGTCCAAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGATATCTGAACTTCCACAAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTTCGGTTTAAGAAGTCCGCGCCCGCGCTGCGACTACACGCCA‑CAATGACCTGTTAATGAGATTCGCGGGCTTTTTTTTCTTTCTAAACCATCGCCGTTCCgtcgtt                                                                                                                                                                                                  >  2:76552/1‑246 (MQ=255)                                            cgcTGATTGGCAAACTTCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtt                                                                                                                                                                                                  <  1:13649/251‑1 (MQ=255)                                             gcTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt                                                                                                                                                                                                 <  1:133903/251‑1 (MQ=255)                                              gtGATTGGAAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt                                                                                                                                                                                                <  2:191102/250‑1 (MQ=255)                                                    ggCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTAAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCAGGGCCTTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCg                                                                                                                                                                                          >  1:260056/1‑251 (MQ=255)                                                    ggCAAAATGACGAAGCCGGAAGAGTACAAGACCTACGTGGCGCAGGTAGATAAAAACGCCGATGATACTTACGGTAGTCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGTGTGATTTGTCAGACTGCGGTGTTAACAGGCAGCCCACGCCCGGCGCATAAAC‑ACAACATAGCCAGGATAATGAGATTCCCGGGCATTTTGTTATTACTAAACCATCTCCAGTCCGCTACTTTtctc                                                                                                                                                                                            >  2:26391/1‑249 (MQ=255)                                                            ggcGACGCCGGAAGAGTACCAGCCCTACGTGGCGCATGTAGATAAAACAGCCGTTGATACTTACCGTTATCTTAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCt                                                                                                                                                                                  <  2:534755/249‑1 (MQ=255)                                                               gACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCg                                                                                                                                                                               >  2:613637/1‑251 (MQ=255)                                                                 cGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGAt                                                                                                                                                                             >  1:234833/1‑251 (MQ=255)                                                                        agagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCAttttt                                                                                                                                                                                                                                >  1:150836/1‑193 (MQ=255)                                                                        agagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCAttttt                                                                                                                                                                                                                                <  2:150836/193‑1 (MQ=255)                                                                                agtcctactgggctcagggaactAAAACAGCTGTGGATACTTACCGTTTACTGAAATTCAACCAGCTGTCCCAGTACACGGAGAAAGCCGAGGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCACCGCACGCGCTGCGCTTAAACGACA‑CCATGCCCGGTTAATTAGATTCCCGGGCAGTTTTTTAGGGCTAATCCATCGCCTTTCCGCTTTGTGTCTCCGGTAAGGCTGCTATAATTACATCAATg                                                                                                                                                                <  2:419968/227‑1 (MQ=255)                                                                                          ggCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGAt                                                                                                                                                    >  1:517496/1‑251 (MQ=255)                                                                                                ggTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGt                                                                                                                                              <  1:594748/251‑1 (MQ=255)                                                                                                                  tGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCag                                                                                                                              >  1:2907/1‑249 (MQ=255)                                                                                                                   gatactctcagTTATCTGAACTTCAACCAGCTTTCTCAGTACAACGAGAACGCCGATGGGGTGATTTTCCAGGCTGCGGGTTAAAAAGTCAGCGCACGTGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCACCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATGTCGGTGCATGGACGGGAGCagag                                                                                                                            <  2:496716/240‑1 (MQ=255)                                                                                                                       cTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAac                                                                                                                        >  1:120328/1‑250 (MQ=255)                                                                                                                                    gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGAtt                                                                                                            <  2:149217/249‑1 (MQ=255)                                                                                                                                    gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTAc                                                                                                          <  1:613637/251‑1 (MQ=255)                                                                                                                                      aCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACg                                                                                                         >  1:239548/1‑250 (MQ=255)                                                                                                                                         tCAACCAGCTTTCGCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAAt                                                                                                      <  2:517496/250‑1 (MQ=255)                                                                                                                                          cAACCAGCTTTCTCAGTACTCCGAGAAAGCCGATGGGGTGATTTTCCAGGCTGCGGTTGAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGAGTCCCGGGCATTTTTTTATTTCTAAACCAACGCCGGTCCGCTGGTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATGTCGGTGCATTGCCGGGAGCAGATGAACACACTTTCGATTACGaagtt                                                                                                    <  2:572975/251‑4 (MQ=255)                                                                                                                                          cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAAttt                                                                                                    <  1:470295/251‑1 (MQ=255)                                                                                                                                               aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTgcg                                                                                               >  1:35110/1‑251 (MQ=255)                                                                                                                                                gCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGAGCATTGCCGGGAGAAGCGGAACACACTATGGCTTACGCATTTCTgcgc                                                                                              >  2:386774/1‑251 (MQ=255)                                                                                                                                                    tctcAGTACACCGGGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTACTGAGATTCCCGGGCATTTTTTTATTTCTAACCCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGCTTTCGGTGCATTGCCGGGAGCAGAGGAACACGCTATGGATTACGAATTTCTGCGCGat                                                                                           >  1:47866/1‑250 (MQ=255)                                                                                                                                                           acacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCgg                                                                                   <  1:580839/251‑1 (MQ=255)                                                                                                                                                             acCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATTGATTACGAATTTCTGCTCGATATTACCGGAg                                                                                 <  2:654676/251‑1 (MQ=255)                                                                                                                                                                gagaAAGCCGATGGGGTGATTTTCCAGAATGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCAGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTgg                                                                              >  1:215974/1‑251 (MQ=255)                                                                                                                                                                     aGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTaa                                                                           >  1:22482/1‑249 (MQ=255)                                                                                                                                                                                                gggttAAAAAGTCCGCGCTCGCGCTGCTCATAAACGACC‑CAATGCCCGGTTAATGAGACTCCCGGTCATTTATTTATTTCTAAACCATCGCCGTCCCGCTGTTTTTCGCCGGTAAGGCTGCGATAATTACATCTATGGCGCAATGCGATTTCGGTGCATTGCCGTGAGCAGAGGAACACATTATGCATTACGAATATCTGCTCGATATGACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGtt                                              <  2:710460/248‑2 (MQ=255)                                                                                                                                                                                                     aaaaaGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCgg                                         <  1:83262/251‑1 (MQ=255)                                                                                                                                                                                                         aGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCAc                                     >  2:238378/1‑251 (MQ=255)                                                                                                                                                                                                            cAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTg                                   <  1:6278/250‑1 (MQ=255)                                                                                                                                                                                                                gcACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGCGATTCCCGGGCCTTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGCTTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGTTATTACCGGTGTGGTAAAGGTGCGTATGTCACTGGGGCCTGAAGGGGCCGGGCACTGGttt                               >  2:690140/1‑250 (MQ=255)                                                                                                                                                                                                                gcACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGCTTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGttt                               >  1:302978/1‑250 (MQ=255)                                                                                                                                                                                                                gcACGCGCTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGttt                               >  2:5912/1‑250 (MQ=255)                                                                                                                                                                                                                     cgcTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCTTGAAGTGGTCGGGCACTGGTTTAATGa                          >  1:180425/1‑250 (MQ=255)                                                                                                                                                                                                                     cgcTGCGCATAAACGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGaa                         <  2:358155/251‑1 (MQ=255)                                                                                                                                                                                                                               aaaCGACA‑CAATGCTCGGTTAATGAGATTCTCGGGGATTTTTTTATTTATAAACCATCGACGTTAAGATGTTTTTCTCCGGTAAGGCTGCGTTAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACTCTCTGGATTACGAATTTCTGCGCGATCTTACCGGAGTGGTAAAGGTGCGTATGTCCATGGAGCATGAATGGGTCGGGCACTGGTTTAATGTAGAGGTgaa                 >  2:618268/1‑249 (MQ=255)                                                                                                                                                                                                                                aaCGACA‑CAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGACTTTCTGCGCGATCTTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTgaatg               >  1:531598/1‑248 (MQ=255)                                                                                                                                                                                                                                        caATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTg       <  1:351761/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaggCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACCCACTATGGATTACGAATTTCTGCGCGATATTATCGGAGTGGTAAAGGTGCGTATGTTCATGGGGCATGAAGTGGTTGGGCACTGGtt                                <  2:612622/222‑1 (MQ=255)                                                                                                                                                                                                                                         aaTGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGGCGGGCACTGGtt                                >  1:612622/1‑225 (MQ=255)                                                                                                                                                                                                                                             cccGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt  <  1:386774/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GCGAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACA‑CAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTT  >  NC_000913/134015‑134500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑