Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,471	T→G	intergenic (+439/‑163)	cra → / → mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,471	0	T	G	100.0%	153.6 / NA	49	intergenic (+439/‑163)	cra/mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/24);  total (25/24)

TTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCGTTATCGGGAACAGCTGCTTG  >  NC_000913/89228‑89715                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ttattacctgcagagATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCACCAGGGACTGTTAACCGGGGCTGATATGTCCTAAAATGCCGCTCGCGACGCAAACTGCCACTTTCTATTTGCTGAGGATAATCGCGAGTCCTTTTAAACCGGCGCTGACGATGTGGGATTTTTTCTTACAGCTCTTCATAACGTTAATTTACTGCGCACGt                                                                                                                                                                                                                                                >  2:277474/15‑251 (MQ=255)     tACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTgg                                                                                                                                                                                                                                             <  2:683494/250‑1 (MQ=255)       catgcagagATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACg                                                                                                                                                                                                                                          >  1:160753/9‑251 (MQ=255)          gcagagATGAATTGCCGATTGAATAAGCACGTTTCTTTGCTTGTGCGCAAACCAGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTGAACCGGGGAAGATATGTCCTAAAATTCCGCTCGCGTCGCAAACTGACTCTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGGTAATTTGCTGCGCCCGTTGGACGTa                                                                                                                                                                                                                                        <  1:445714/245‑1 (MQ=255)            agagATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAACACGGTGATGACGATGAGGGCTTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTaaa                                                                                                                                                                                                                                      >  2:350930/4‑250 (MQ=255)             gagATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAAt                                                                                                                                                                                                                                    <  2:299418/249‑1 (MQ=255)                   aaTTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAaaca                                                                                                                                                                                                                               >  1:592363/1‑250 (MQ=255)                                accagcacTTTGCGTTGCGTTTGCGCAAACCCTCGGGCATCAAGCTCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTTTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACACCGCTTATATTAGc                                                                                                                                                                                                                 <  2:125536/246‑1 (MQ=255)                                 caagcacTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGCCGCAACAGGGACTGTTAACAGGGGCAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTCTATTTCCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTATTACAGCTATTCATACCGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGcc                                                                                                                                                                                                                >  2:674625/5‑251 (MQ=255)                                         tttCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAAc                                                                                                                                                                                                          <  1:682166/249‑1 (MQ=255)                                                gCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCCACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAACACGATGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTGAACATCGGGttt                                                                                                                                                                                                 >  1:280303/1‑251 (MQ=255)                                                                gCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGcc                                                                                                                                                                                 >  2:553199/1‑251 (MQ=255)                                                                  ggtcATCAAGCGCCACACAGACGCAACAGTGAATGTTAACTGGGGAATATATGTCCTAAAATGCCGCTCGCGTTGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTTATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCtt                                                                                                                                                                               <  2:438105/248‑1 (MQ=255)                                                                       tCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGAc                                                                                                                                                                          >  1:521643/1‑251 (MQ=255)                                                                             gcCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGCTTGCCTTGTGACTGGCtt                                                                                                                                                                    >  2:362749/1‑251 (MQ=255)                                                                                acacaGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGa                                                                                                                                                                  >  2:281986/1‑250 (MQ=255)                                                                                                                         cTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAAAGTTAATTTGATGCGCACGTGGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGAATGGCTTGACAAGCTTTTCCTCCCCTCCGTAAACTCCTTTCAGTGGGGAAt                                                                                                                        >  2:553947/1‑251 (MQ=255)                                                                                                                              aTGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgg                                                                                                                   >  1:100247/1‑251 (MQ=255)                                                                                                                                      cgcgTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCgg                                                                                                                                                                                                     <  1:486024/161‑1 (MQ=255)                                                                                                                                      cgcgTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCgg                                                                                                                                                                                                     >  2:486024/1‑161 (MQ=255)                                                                                                                                        cgTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTgg                                                                                                         >  1:198872/1‑251 (MQ=255)                                                                                                                                           cGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGa                                                                                                       <  2:198872/250‑1 (MQ=255)                                                                                                                                                            tatTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGAATAAGGGGGTAGGCTggg                                                                                     >  2:636127/1‑250 (MQ=255)                                                                                                                                                             ctttGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCtt                                                                                                                                                                               >  2:297928/2‑160 (MQ=255)                                                                                                                                                             atTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCtt                                                                                                                                                                               <  1:297928/160‑1 (MQ=255)                                                                                                                                                                tgatttgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGCGGGATTTTCTCTTTCAGTTATTCATAACGATAATTTTCTGCGCACGTTGGACGTAAAATAAACAACGCTGATAGTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTGGATAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGTGTAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATg                                                                                  <  2:2213/245‑1 (MQ=255)                                                                                                                                                                 gCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGtt                                                                                <  2:363228/251‑1 (MQ=255)                                                                                                                                                                                 aGTCATTTTAAAACGGTGAGGTCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGTTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCGCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCGAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTa                                                                <  1:277474/251‑1 (MQ=255)                                                                                                                                                                                   tCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGaa                                                                                                      >  1:267290/1‑211 (MQ=255)                                                                                                                                                                                   tCATTTTAAAACGGTGAGGATGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGaa                                                                                                      <  2:267290/211‑1 (MQ=255)                                                                                                                                                                                    cATTGTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCTGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCGTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTc                                                             <  1:395060/251‑1 (MQ=255)                                                                                                                                                                                        ttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAt                                                          <  1:332361/250‑1 (MQ=255)                                                                                                                                                                                         tAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGTCGTAAAATAAACAACGCTGATATTAGCCGTAATCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGTCAAGCTTTTCCTCCGCTCCGTAAACGCCGTTCAGTGGGAAAGTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCAGGTTCCGGGGAGCAACGTTAGTCAAtc                                                         <  2:605733/250‑1 (MQ=255)                                                                                                                                                                                                gTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGc                                                 <  1:432902/251‑1 (MQ=255)                                                                                                                                                                                                   atgaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCaa                                               >  1:39414/1‑250 (MQ=255)                                                                                                                                                                                                      aCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAggg                                           <  1:124246/251‑1 (MQ=255)                                                                                                                                                                                                        gATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTACGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGGGCAACGGTAGTCAATCTCGTAAGCAAAggggg                                         >  2:378222/1‑249 (MQ=255)                                                                                                                                                                                                               gATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑Ca                                 <  1:669736/251‑1 (MQ=255)                                                                                                                                                                                                                      tCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGTAATTTTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCtt                          >  1:34511/1‑250 (MQ=255)                                                                                                                                                                                                                         tACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGCTATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCCAAGTGGGAATAAGGGGTGTGGATGGGATGTTCCGGGGAGCAACGATAGTCAATCTCGACAGCAAAGGGCGCT‑ATACAGTGCCAAccc                       >  2:169423/1‑251 (MQ=255)                                                                                                                                                                                                                          aCAGCTATTCCTAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGCGGGAATACGGGGTGAGGCTGGACTGTTCCGGGGGGCAACGTTAGTCACACTCGACAGCAAAGGGCGCGTAT‑CAGGGCCtccagg                      >  2:329799/1‑246 (MQ=255)                                                                                                                                                                                                                          aCAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATACACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCCGTGGGGAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCGGGCATGTTCCGGGGAGCAACATTAGTCAATCTCGACAGCAAAGGGCGATTAT‑CCGTGCCTAcccc                      >  2:348869/1‑250 (MQ=255)                                                                                                                                                                                                                          aCAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTAc                         <  1:329799/248‑1 (MQ=255)                                                                                                                                                                                                                          aCAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCg                      <  1:281986/251‑1 (MQ=255)                                                                                                                                                                                                                            aGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGAAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCGTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCGtt                    <  2:367687/251‑1 (MQ=255)                                                                                                                                                                                                                                aTTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTTGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCGTTaccg                >  1:626130/1‑248 (MQ=255)                                                                                                                                                                                                                                        cGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCGTTACCGGGAACAgc        <  1:360482/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaTTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGTAAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCCAAGTGGGAATAAGGGGGGAGGCTGGCATGTTCCGGGGGGCCACGTTAGTAAATCTTGGAAGCAAAGGGGGCTTAT‑AAGTGCCTACCCGTTACCGGGGACAtctgct    >  2:519408/1‑251 (MQ=255)                                                                                                                                                                                                                                               ttGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCGTTACCGGGAACAGCTGCTTg  <  2:592363/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      TTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAT‑CAGTGCCTACCCGTTATCGGGAACAGCTGCTTG  >  NC_000913/89228‑89715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑