Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,539	T→C	S414S (TCA→TCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,539	0	T	C	93.1%	174.4 / ‑2.8	59	S414S (TCA→TCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (20/34);  total (21/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTC  >  NC_000913/64293‑64772                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            catgatattgtccAGCGCCTGAGAGAGCGGTTGGTTACCCTTGCGTTTGGCTTCATCGCGCTCGTGCCAGATGT‑AAGTCTCAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAGCCAGGCAACGAGAAATCCTTCGGTACTGTGCTCTGGATTAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGTCGGGTACAGGCTTTTATAGTCCAGCGCCAGTACC‑GAAt                                                                                                                                                                                                                                        <  2:501780/239‑1 (MQ=255)  atgatgATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATc                                                                                                                                                                                                                                       <  1:517295/251‑1 (MQ=255)       gatTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATaaa                                                                                                                                                                                                                                  <  1:362582/251‑1 (MQ=255)           ttCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGcc                                                                                                                                                                                                                               <  1:593641/250‑1 (MQ=255)                        gcAGCGGTTTGTTTCCCTGGCGTTTGGATTCATCGCGTCCGGGCCAGA‑GTGTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGTCCGTGAATcc                                                                                                                                                                                                                <  2:479670/250‑1 (MQ=255)                        aCAGCTGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATcc                                                                                                                                                                                                                <  1:667366/251‑1 (MQ=255)                          aGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCAt                                                                                                                                                                                                              >  1:388378/1‑251 (MQ=255)                                 tGTTCCCCTGGCTCTTGGCCTCTTCGCTCCCGTTCCAGATGT‑TATTCACAATCCCCCGCAGGCCATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCGTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGTACCAGTACC‑GAATCAGAAAGCCC‑TGGCCGTGAATCCATCACGTa                                                                                                                                                                                                        <  2:282044/250‑1 (MQ=255)                                  gTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGCTCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGGGAATCCCACACGTAgc                                                                                                                                                                                                      >  2:701523/1‑251 (MQ=255)                                    tACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAgcc                                                                                                                                                                                                     <  1:186840/250‑1 (MQ=255)                                         gggCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAgg                                                                                                                                                                                               <  1:290161/250‑1 (MQ=255)                                          ggCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGGCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGGGAATCCATCCCGTAGCCGCCAg                                                                                                                                                                                                <  2:497949/249‑1 (MQ=255)                                           gCGTTTGGCTTCATCGCGCCCGGGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGc                                                                                                                                                                                             <  2:556161/251‑1 (MQ=255)                                            cGTTTGGTCTCAACGCGCCCGTGCCAGATGT‑TAGTCAAAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGAATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCt                                                                                                                                                                                            <  2:173739/251‑1 (MQ=255)                                                 ggCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGt                                                                                                                                                                                       <  1:245639/251‑1 (MQ=255)                                                     tCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCCGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTgtg                                                                                                                                                                                    <  2:619439/250‑3 (MQ=255)                                                           cgcCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCAc                                                                                                                                                                             >  1:330005/1‑251 (MQ=255)                                                             cccGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAACCGCCAGGGCTGGCGTgtgggtgcactt                                                                                                                                                                           >  2:656035/1‑240 (MQ=255)                                                                       aTGT‑TAGTCACAATCCCCGGCAGGCCAGGTTTTTCTCGCGAGACCCAGTCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCTTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAtt                                                                                                                                                                 <  2:543654/251‑1 (MQ=255)                                                                              gTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGgcgc                                                                                                                                                           <  1:608797/251‑1 (MQ=255)                                                                                  caATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAAACTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGAcc                                                                                                                                                       >  1:59828/1‑250 (MQ=255)                                                                                        ccGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATaa                                                                                                                                                    <  1:409718/248‑1 (MQ=255)                                                                                         cggccggcAATGTTTTTCTCGCGAGAACCAGGAATCGAGAAAACCTTCGGCACTAAGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCCGAAAGGGGCGGATGATAGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑GGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGAGGGGCGGCACTTCGCCGGGATTAGGCGGGACATAAcc                                                                                                                                                  >  1:633532/1‑249 (MQ=255)                                                                                         cGGCAGGCAATGTTTTTCTCGCTAGAACCAGGCATCGAGAAAACCTTCGGTACTTTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAg                                                                                                                                                <  1:562324/251‑1 (MQ=255)                                                                                           gcaggcaATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCCCCGGGATTAGGCGCGACATACCCAgcg                                                                                                                                              >  2:502053/1‑251 (MQ=255)                                                                                                  aTGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACAGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCa                                                                                                                                       <  1:605497/251‑1 (MQ=255)                                                                                                                agaACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATag                                                                                                                         <  1:685354/251‑1 (MQ=255)                                                                                                                    ccAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCAtacgagg                                                                                                                                <  1:189219/240‑7 (MQ=255)                                                                                                                    ccAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCAtacgagg                                                                                                                                >  2:189219/1‑234 (MQ=255)                                                                                                                          aTCGAGAAAACCTTCGGTACTGTGCCCTGGATCAGGCCGCGCCTTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGTCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGACAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaa                                                                                                               <  2:388378/251‑1 (MQ=255)                                                                                                                            cGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGAAGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCTCGTAGCCGCCAGGGCTGGCGTGTGGCTGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaaac                                                                                                             <  1:324449/251‑2 (MQ=255)                                                                                                                             gagaAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCaaacg                                                                                                            >  1:144743/1‑249 (MQ=255)                                                                                                                                     tttCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCCCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAACCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAc                                                                                                     <  2:557999/249‑1 (MQ=255)                                                                                                                                      ttCGGTACTGTGCTCTGGATCAGGCTGCGCCCTGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAc                                                                                                     >  1:557999/1‑249 (MQ=255)                                                                                                                                           tACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGAACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGACTCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGAGCGACATAACCAGCGCGGGGCATACGGGGAAAATAGAAGTGACCAACCGCCGCCACCGatccc                                                                                              >  2:339844/1‑247 (MQ=255)                                                                                                                                               gtgtgcgggATAAGGCTGCGCAAGGCTTTCCCCCAGACCGACGGGATCAACCAGAAAGGTGCGGATGAGAGACGGGTACAGGCTTTTATAGTCCAGCACCAGCCCT‑GAATCATAAAGCCC‑TGGCCGTGAATCAATCACGTAGCCGCCAGGGCGGGCGTGCGGCGGCACTGCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCGCCGAAccgcc                                                                                            <  2:53577/242‑1 (MQ=255)                                                                                                                                               gtgCTCTGGATCAGTCGGGGCCCTGCCTTCCCCCAGCCCGACGTGATCAATCCGAGATGTGCGGAGTATCGACGGTTCCAGGCTTTTATAGTCCAGCACCCTTACC‑TAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACAGAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCTGCCACCGatcccccg                                                                                           <  2:430945/250‑8 (MQ=255)                                                                                                                                                       gATCAGGCTGCGCCATGCCTTCCTCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTct                                                                                  <  1:476642/251‑2 (MQ=255)                                                                                                                                                           aGGCTGCGCCATGCCTGCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGAGGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCg                                                                              <  2:252097/251‑1 (MQ=255)                                                                                                                                                             gtcgcgcCTTGCCGTCCACCAGCCCGACGGGATCAATCAGAAGGTTTCGGATGATCGACGGGGACTGGCTTTTATAGTCCAGCACCAGTACC‑GAATCAAAAAGCCC‑TGTCCGTGAATCCATCACGTAGCCGCCAGTGCTGTCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACTAGGAAAATAGATATGACCAAACGCCGCCACCGatcccctgtggcggtcttccggc                                                                            <  2:248971/248‑23 (MQ=255)                                                                                                                                                              cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTAcc                                                                               <  1:97250/247‑1 (MQ=255)                                                                                                                                                              cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCAAccagtg                                                                           >  2:97250/1‑247 (MQ=255)                                                                                                                                                               tGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGc                                                                            >  1:171947/1‑249 (MQ=255)                                                                                                                                                                            ccaccgGCCCGGCGGGATCAATCAGAAAGGGGCGGAGGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAAGCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGACGTTCACCGtc                                                             <  2:317089/251‑2 (MQ=255)                                                                                                                                                                             caccaGCCTGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcg                                                            <  2:240962/251‑3 (MQ=255)                                                                                                                                                                              cccaGCCCGACGTGATGAATCAGAAAGGTGCGGATGACCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGTATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcgc                                                           <  1:321135/250‑4 (MQ=255)                                                                                                                                                                                   cccaagggtatcaaacaAAAAGGGGCGGAGGATCGAGGGGTACAGGCTTTTAGAGCCCAGCAC‑AGGACTTAAATCAAAAGGCCC‑GGCCCGCGAATACATCAGTTAGCCCCCAGGGCGGGCGGGCGGGGCCACTTCGCGGAGATTAGGCGCGACAAACCCAGCGCGATGTATTCGCGAAAAATAGAGTGGACCAAATGCCGCCCCCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGtt                                                      <  2:238911/242‑1 (MQ=255)                                                                                                                                                                                       aCGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGatcccccg                                                                                           <  1:454067/210‑8 (MQ=255)                                                                                                                                                                                         gggATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGt                                                 >  1:271167/1‑250 (MQ=255)                                                                                                                                                                                            atcaatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCAAGGAGCCGCCAGGGCTGGCGTGCGGCGGCCATTCGCCGAGATTCGGCGCGTCATAAACAGCGCGATGCATTCGCGGACAATAGAGATGATCAAGAGCCGCCAACGAACCACCGTGTCGGTACACCGGCAGGCCGTTCACCGTTGcccctccaaggcaa                                              >  2:157314/1‑239 (MQ=255)                                                                                                                                                                                                 tcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCAcccgccctcgttcggtgtacaatggc                                        >  2:630101/1‑227 (MQ=255)                                                                                                                                                                                                     aaaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGACCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGa                                    <  1:229414/251‑1 (MQ=255)                                                                                                                                                                                                      aaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGAt                                   >  1:124074/1‑251 (MQ=255)                                                                                                                                                                                                           gCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCa                               <  1:298861/250‑1 (MQ=255)                                                                                                                                                                                                              gatgagagACGGGTACAGGCTTTGATAGTCCAGCACCAGTACA‑GAATCATAAAGCCCGTGGC‑GTGAATCCATCACGGAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTATGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAACGATCCCCCGTGGAGGTCTACCGGCAGGCCGTTCACCGTCGCGCGTTCGAGTAAAAATGGCATGATTTCAGttt                           <  2:104982/244‑1 (MQ=255)                                                                                                                                                                                                                               ggCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTc          >  1:470418/1‑251 (MQ=255)                                                                                                                                                                                                                                 cTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGCTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCCGTTTTATgggaaaaccgcgtcac        >  2:190035/1‑237 (MQ=255)                                                                                                                                                                                                                                  ttttATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCAcc       >  1:363444/1‑251 (MQ=255)                                                                                                                                                                                                                                       taGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATCCGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGATCACCGGCGCTCGTTCGGGTAAAAAAGGCCTTATTTCCGTTTTATTGAAAATCTGCGTCACCaacac  >  2:555262/1‑247 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGT‑TAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTC  >  NC_000913/64293‑64772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑