| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 86,315 | C→T | T229M (ACG→ATG) | ilvI → | acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 86,315 | 0 | C | T | 95.2% | 166.8 / NA | 65 | T229M (ACG→ATG) | ilvI | acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (26/33); minor base A (3/0); total (29/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.66e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTC > NC_000913/86069‑86561 | ggctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTTCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTg < 1:501782/251‑1 (MQ=255) gctggcGGGAAGTGGTCGCCCAGGAGCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAAGCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGCTGCCAGCAGCAGTTGAAAGAAAtggtgg < 2:267284/251‑1 (MQ=255) ggaggcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATAGTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGGAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAg < 2:189807/247‑1 (MQ=255) ggTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGGG‑GTGAATCt > 1:705956/1‑251 (MQ=255) ggTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCt < 1:569414/251‑1 (MQ=255) cAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCgtt > 1:178211/1‑251 (MQ=255) aGGACCAGTAGTCGTTGAGTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCgttg < 2:6825/251‑1 (MQ=255) ccAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCTGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTGCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAAGCTGCCCGTTGtttc < 2:378666/251‑2 (MQ=255) ccAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGtttc < 1:462015/251‑2 (MQ=255) cAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACACTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCACACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGACACCCGCAATTGAAAGAATAGGTGGGGGCG‑TTGGATCTGCCCgttggttcc > 1:640328/1‑246 (MQ=255) cAGTAGTCGTTGATGTACCGAAAGATACTCTTAAACCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGGCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGtttcc < 2:485463/251‑3 (MQ=255) agtCGTTGTATTACCGAAAGATATTCTTAATCAGGCGACCAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTCCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGTCAAAAAAACCGGTTGTCTACGTAGGTGGTGGCGCAATCTCGGCGGGATGCCATAAGCACTTTAAAGCAAAGTTGGGGGCG‑TTGAATCTGcccattttctacccct > 2:264335/1‑238 (MQ=255) aGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTgggggggg > 1:359632/1‑248 (MQ=255) tatTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAACAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAACTTAAAGAACTGGTGGAGGGGGTTGA‑TGTGCCCGTTGTTTTCTCTTTGAGGTGGCTGGGGGGGTTTc > 2:179063/1‑251 (MQ=255) tCTTAATCCGGCGAACAAATTACCCTATGGCTGGCCGGAGTCGGTTAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGTGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCGGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCg < 2:440339/250‑1 (MQ=255) ttAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGGAAGAACTGGGGGCGGCG‑TTTAATCTGCCCGTTTTTTCCTCATTGGTTGGGGTGGGGGCGTTTTCGGc > 2:113398/1‑250 (MQ=255) tACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACt < 1:277638/250‑1 (MQ=255) cccTATGTCTGGCCGGAGTCGGTCAGTTAGCGTTCTTACAAGCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCATCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTggg < 1:92836/251‑1 (MQ=255) ggAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAAAAATGGTGGTGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTTATGGGGCTGGGGGGGTTTCCGGCAACGCATCGTCAGGCACTGGGGATTCTGGGAATG‑c > 1:283674/1‑251 (MQ=255) gTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCAt < 1:627425/184‑1 (MQ=255) gTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGGGGCG‑TTGGATCTGCCCGTTGTTTTCTCAt > 2:627425/1‑184 (MQ=255) aCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATg < 1:125299/251‑1 (MQ=255) caagcccACTGCTACCGGACATAAAGGGCAAATTAATCGTGCGCTGCAAACGCTGGTGGCGGCAAAAAAACCGGGTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGa < 2:270761/247‑1 (MQ=255) gccctctacgaCCGGAAATAAAGGGCAAATTAAGCCGGCGCTGCAAATGCTGGGGGCGGCAAAAAAACTGGGTGTCGTCGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAGTAGCGTTTGAAAGAAAGGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGCTGGGGCTGGGGGCGTTTCCGGCAACGCATCGTTAGGCACGGGGCATGCTGGGAATG‑CACGTTACCTGCGAGGCCAATATGACGa < 2:467868/246‑1 (MQ=255) cACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGc < 2:178211/251‑1 (MQ=255) actactACCGGACCAAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTTGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCa < 2:334546/251‑1 (MQ=255) cGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGGAATGGGGGGGGCG‑TTGAATCTGGCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCCACGCATCGTCAGGCACTGGGGCTGCTGGGGATG‑AACGGTACCTACGATGCCAGAAAGACGAGGCATaaacggg > 2:100663/1‑246 (MQ=255) aaaGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCGGCCATCCGCACTTGAAAAACATGGTGGGGGGG‑TTGAATCTGCCCGTTGTTTCTTCATTGTTGGGGCTGGGGGGGGTTCCGGCAACGCATCGTCCGGCACTGGGCATGCTGGGAATGGCA‑GGGACCCACACACCCAATATTACGATGCATAAAGCggtgggtgt > 2:245958/1‑244 (MQ=255) aaaGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGATGCCCTCAGCAGTTGAAAGAAAAGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTTCTTATTGATGGGGCTGGGGGGGTTTCCGGCAACGCATAGTTAGGACCTGGGGATGATGGGAATG‑CACGGGAACCACGAAGCCAATATGTTGATGGATAACGCGGATGTGAt > 2:161878/1‑251 (MQ=255) aaaTTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGGTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCCTGCTGGGGATG‑CACGGTACCTACGAAGCCAATAAGAAGCTGCATAACGCGGATGTGATTTTAGcc > 1:562425/1‑251 (MQ=255) ttAAGCGTGCCCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAACAGCAGTTGAAAGACATGGGGGAGGCG‑TTGGATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGAACTGGGAATGCTGGGAATG‑GACGGTCACTACGAAGCCAAAATGACGATGCATAACGCGGGAGTGATTTTAGCCggc > 1:682484/1‑249 (MQ=255) tAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGATGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTTCTCATTGATGGGGATGGGGGCGGTTCCGGCAAAGCATCGTCAGGCACTGGGCCTGCTGGGAATG‑GACGGTACCTACGAAGCCAATATGACGAAGCATAACGCGGATGTGGTTTTCggcggcg > 1:662764/1‑245 (MQ=255) aGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCCGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCCTGCTGGGAATG‑CCCGGTACCTACGAAGCCAATATGGCGATGCATAACGCGGATGTGATTTTCGCCGTCggg > 1:242352/1‑251 (MQ=255) tctGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTTCGGCAACGCATCGGCAGGCACTGGGCATGCTGGGAATG‑CACGGTAACTACGAAGCCAATATGACGATGCCTAACGCGGCTTTGATTTTCGCCGTCgggggtcgtt > 1:163593/1‑245 (MQ=255) gccaaCGCTGGCGGCGTCAAAAAAATCGGTTGGTTAAGAAGGTGGTGGGGCAACCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑GTGAATCTGCCCGTGGTTGCCTCATTGATGGGGCTGGGGGCGTTTCCGGCACCGCATCGTCAGGCACGGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGAtgtg < 2:19721/248‑4 (MQ=255) ggtggCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGGACGCTTTGATGACCGa > 1:428038/5‑250 (MQ=255) gtggCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAac < 1:387582/248‑1 (MQ=255) cgatccaaaaacCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAGCAGTAGTTGAAAGAAACGGTGGAGGCG‑GTTAACCTGCCCGTTGTTTGCTCAGTGATGGGTCTGTGGGCGTTTCCGGCAACTCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTCCCTACGAAGCCAATATGACGATGCATAACGCTGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAacgac < 1:312540/245‑1 (MQ=255) cGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATATGATTTTCGCCGGCGGGGTTCGATTTGATGACCGAacgacg > 1:507298/1‑251 (MQ=255) aaaaaaCCGGTTGGCTACGGAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACaa < 2:428038/251‑1 (MQ=255) aaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTg < 1:678704/251‑1 (MQ=255) aaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGAGGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTg < 1:449626/251‑1 (MQ=255) ggTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCaa < 1:480726/251‑1 (MQ=255) gtcgaCGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACTCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGATCAATCTGGCAAAGt < 2:662764/246‑1 (MQ=255) gTCTACGTAGGTGGTGGGGCAATCCCGGCGGGCCGCCATCAGCAGTTGAAAGAAAGGGTGTAGGCG‑TTGAACCTGCCCGTTGTTTCCTCATTGAGGTGGCTGGGGGCGTTTCCGGCAACGCATCGGCAGGCACTGTGCATGCTTGGAATG‑CTCGGGACCTCCGAAGCCAATATGACGATGCATAACGCGGATGTGATGTTCGCCGTCGGGGTACGATTTGAGGACCGAACGTCTATCAATCTGGCAAAGTa < 2:99567/251‑1 (MQ=255) aCGTAGGTGGTGGGGTAATCACGGCTGGCTGCCAGCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAAACGGCCCGGTGTTTCCTCAGTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGGCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGc < 2:527141/251‑1 (MQ=255) gTAGGTGGTGGGGCAATCACGGCGGGCTGACATCAGCAGTTGCAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCAATGGGCATGCTGGGAATG‑CACGGGACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGGTTTTCGCCGTCGGGGGACGATTTGATGACCGAACGACGAACAATCttgccacatactgccc > 1:571943/1‑236 (MQ=255) ggtggTGGGGCAATCACGGCGGGCCGCCATCAGCAGTTTAAAGAAATGGTGGAGGCG‑GTGGATCTGACCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTTCCGCCACGCATCGACCGGGACTGGGCATGGTGGGGATG‑TACGGTACCTACGAAGCCAATATGACGATGCCTCACGCGGATGTGATTTTCGCCGTCGGGGTACGAGTTGATGACCGAAAGGCGAACAATCCTGCAATGTCCTGCCCaa > 2:685228/4‑250 (MQ=255) tggTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTTCTCATTGGTGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGGTTTTCGCCGTCGGGGGAAGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCaaa > 1:423809/2‑249 (MQ=255) ggggCAATCCCTGCGGGCGGCCATCGTCAGTTGAAAGAAATGGTGGGGGCG‑GTGAAGCTGCCCGTTGTTTCCGCATTGATGGGGCTGGGGTCGTTTCCGGCAACGGATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCTCAAATGCCAc < 2:507298/251‑1 (MQ=255) aTCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCt > 1:219215/1‑251 (MQ=255) ccggcggGCTGCCAGCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CCCGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATTCCACTGTTCTGCa < 1:366408/250‑1 (MQ=255) cggcggGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCat < 1:27326/251‑1 (MQ=255) cggGCTGCCATCATCAGTAGAAAGAAATGGTGGATGCG‑TTGAATCTGTCCGTTGTTCCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTTCATATc < 2:571943/251‑1 (MQ=255) gggCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCCTAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGACCGACGAACAATCTGGCAAAGGACTGCCCAAATGCCAATGTTCTGCATATCg > 2:322908/1‑251 (MQ=255) gCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGCATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CCCGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGCTTTTCGCCGTCGGGGGAAGATTTGATGACCGAACGCCGAACAATCTGGCAAAGGACTGCCCAAATGCCCCTGTTCTGCATTACGATAtt > 2:703091/1‑251 (MQ=255) gCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATAtt < 1:11433/251‑1 (MQ=255) cATCAGCAGTTGAAAGAAATGGTGGAGGTG‑GTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGTCATGCTGGGAATG‑CTCGGTACCTACGAAGCCAATATGACTATGCATAACGCGTATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGATCGACGAACATTCTTGCAAAGTACTGCCCAAATGCCTCTGTTCTGCATATCGCTAttta < 2:151873/251‑3 (MQ=255) tCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATATGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGACCTGCCCAAATGCCACTGTTCTGCATATAGATATTGATc > 1:173768/1‑251 (MQ=255) ggcagTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTGGTTTCCCCATTGAGGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGAGGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACTACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCt < 2:359632/250‑1 (MQ=255) agTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGTGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACt < 1:108453/251‑1 (MQ=255) gTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCATGCTCTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACtt < 1:659712/251‑1 (MQ=255) ttGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGAACGATTTGATGACCGAACGACGAACAATCTGGCAAATTACTGCCCAAATGCCACTGATCTGCCTATcattatcactcctacttc > 2:62270/1‑234 (MQ=255) ttGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑AACGGTACCTACGAAGCCAATATGACGATGCATAAAGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGCTGACCGAACGACGAACAATCTGGCAAAGTACGGCCCAAATGCCAGTGATATGCATATCGAAATTGATCCTACtac > 2:214611/1‑249 (MQ=255) tGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTc < 1:113398/250‑1 (MQ=255) gaaaTGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGACCGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCTTATCGATATTGCTCCTACTTCCCTTTc > 2:668585/1‑251 (MQ=255) | GGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTC > NC_000913/86069‑86561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |