Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,271	C→T	G214G (GGC→GGT)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,271	0	C	T	100.0%	163.6 / NA	56	G214G (GGC→GGT)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/30);  total (26/30)

AAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCC  >  NC_000913/86026‑86516                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        aaGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGtggtgg                                                                                                                                                                                                                                                    >  1:273045/1‑251 (MQ=255)        cGGAAGACATTCCGCTGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGGGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATc                                                                                                                                                                                                                                             <  2:204234/251‑1 (MQ=255)         ggAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATc                                                                                                                                                                                                                                             >  1:189807/1‑250 (MQ=255)             gACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCAcggc                                                                                                                                                                                                                                        >  1:527141/1‑251 (MQ=255)                   ccGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCggggtg                                                                                                                                                                                                                                  >  1:19721/1‑248 (MQ=255)                              gAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTCATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTCCTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCGGCCCTcagcagg                                                                                                                                                                                                                       >  1:99567/1‑250 (MQ=255)                                    ggCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaaa                                                                                                                                                                                                                  >  1:370689/1‑250 (MQ=255)                                            ggctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTTCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTg                                                                                                                                                                                                         <  1:501782/251‑1 (MQ=255)                                             gctggcGGGAAGTGGTCGCCCAGGAGCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAAGCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGCTGCCAGCAGCAGTTGAAAGAAAtggtgg                                                                                                                                                                                                        <  2:267284/251‑1 (MQ=255)                                              ctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCt                                                                                                                                                                                                                                   <  1:12889/223‑1 (MQ=255)                                                ggaggcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATAGTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGGAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAg                                                                                                                                                                                                      <  2:189807/247‑1 (MQ=255)                                                          ggTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGGG‑GTGAATCt                                                                                                                                                                                          >  1:705956/1‑251 (MQ=255)                                                          ggTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCt                                                                                                                                                                                          <  1:569414/251‑1 (MQ=255)                                                                 cAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCgtt                                                                                                                                                                                   >  1:178211/1‑251 (MQ=255)                                                                  aGGACCAGTAGTCGTTGAGTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCgttg                                                                                                                                                                                  <  2:6825/251‑1 (MQ=255)                                                                      ccAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCTGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTGCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAAGCTGCCCGTTGtttc                                                                                                                                                                              <  2:378666/251‑2 (MQ=255)                                                                      ccAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGtttc                                                                                                                                                                              <  1:462015/251‑2 (MQ=255)                                                                       cAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACACTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCACACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGACACCCGCAATTGAAAGAATAGGTGGGGGCG‑TTGGATCTGCCCgttggttcc                                                                                                                                                                             >  1:640328/1‑246 (MQ=255)                                                                       cAGTAGTCGTTGATGTACCGAAAGATACTCTTAAACCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGGCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGtttcc                                                                                                                                                                             <  2:485463/251‑3 (MQ=255)                                                                           agtCGTTGTATTACCGAAAGATATTCTTAATCAGGCGACCAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTCCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGTCAAAAAAACCGGTTGTCTACGTAGGTGGTGGCGCAATCTCGGCGGGATGCCATAAGCACTTTAAAGCAAAGTTGGGGGCG‑TTGAATCTGcccattttctacccct                                                                                                                                                                         >  2:264335/1‑238 (MQ=255)                                                                                 tGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCAcggc                                                                                                                                                                                                                                        >  1:387404/1‑183 (MQ=255)                                                                                 tGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCAcggc                                                                                                                                                                                                                                        <  2:387404/183‑1 (MQ=255)                                                                                             aGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTgggggggg                                                                                                                                                       >  1:359632/1‑248 (MQ=255)                                                                                                tatTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAACAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAACTTAAAGAACTGGTGGAGGGGGTTGA‑TGTGCCCGTTGTTTTCTCTTTGAGGTGGCTGGGGGGGTTTc                                                                                                                                                    >  2:179063/1‑251 (MQ=255)                                                                                                   tCTTAATCCGGCGAACAAATTACCCTATGGCTGGCCGGAGTCGGTTAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGTGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCGGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCg                                                                                                                                                  <  2:440339/250‑1 (MQ=255)                                                                                                     ttAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGGAAGAACTGGGGGCGGCG‑TTTAATCTGCCCGTTTTTTCCTCATTGGTTGGGGTGGGGGCGTTTTCGGc                                                                                                                                                >  2:113398/1‑250 (MQ=255)                                                                                                                       tACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACt                                                                                                                              <  1:277638/250‑1 (MQ=255)                                                                                                                         cccTATGTCTGGCCGGAGTCGGTCAGTTAGCGTTCTTACAAGCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCATCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTggg                                                                                                                           <  1:92836/251‑1 (MQ=255)                                                                                                                                       ggAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAAAAATGGTGGTGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTTATGGGGCTGGGGGGGTTTCCGGCAACGCATCGTCAGGCACTGGGGATTCTGGGAATG‑c                                                                                                            >  1:283674/1‑251 (MQ=255)                                                                                                                                              gTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCAt                                                                                                                                                                         <  1:627425/184‑1 (MQ=255)                                                                                                                                              gTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGGGGCG‑TTGGATCTGCCCGTTGTTTTCTCAt                                                                                                                                                                         >  2:627425/1‑184 (MQ=255)                                                                                                                                                              aCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATg                                                                                     <  1:125299/251‑1 (MQ=255)                                                                                                                                                               caagcccACTGCTACCGGACATAAAGGGCAAATTAATCGTGCGCTGCAAACGCTGGTGGCGGCAAAAAAACCGGGTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGa                                                                                    <  2:270761/247‑1 (MQ=255)                                                                                                                                                                  gccctctacgaCCGGAAATAAAGGGCAAATTAAGCCGGCGCTGCAAATGCTGGGGGCGGCAAAAAAACTGGGTGTCGTCGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAGTAGCGTTTGAAAGAAAGGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGCTGGGGCTGGGGGCGTTTCCGGCAACGCATCGTTAGGCACGGGGCATGCTGGGAATG‑CACGTTACCTGCGAGGCCAATATGACGa                                                                                 <  2:467868/246‑1 (MQ=255)                                                                                                                                                                     cACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGc                                                                              <  2:178211/251‑1 (MQ=255)                                                                                                                                                                      actactACCGGACCAAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTTGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCa                                                                             <  2:334546/251‑1 (MQ=255)                                                                                                                                                                              cGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGGAATGGGGGGGGCG‑TTGAATCTGGCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCCACGCATCGTCAGGCACTGGGGCTGCTGGGGATG‑AACGGTACCTACGATGCCAGAAAGACGAGGCATaaacggg                                                                     >  2:100663/1‑246 (MQ=255)                                                                                                                                                                                     aaaGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCGGCCATCCGCACTTGAAAAACATGGTGGGGGGG‑TTGAATCTGCCCGTTGTTTCTTCATTGTTGGGGCTGGGGGGGGTTCCGGCAACGCATCGTCCGGCACTGGGCATGCTGGGAATGGCA‑GGGACCCACACACCCAATATTACGATGCATAAAGCggtgggtgt                                                              >  2:245958/1‑244 (MQ=255)                                                                                                                                                                                     aaaGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGATGCCCTCAGCAGTTGAAAGAAAAGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTTCTTATTGATGGGGCTGGGGGGGTTTCCGGCAACGCATAGTTAGGACCTGGGGATGATGGGAATG‑CACGGGAACCACGAAGCCAATATGTTGATGGATAACGCGGATGTGAt                                                              >  2:161878/1‑251 (MQ=255)                                                                                                                                                                                            aaaTTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGGTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCCTGCTGGGGATG‑CACGGTACCTACGAAGCCAATAAGAAGCTGCATAACGCGGATGTGATTTTAGcc                                                       >  1:562425/1‑251 (MQ=255)                                                                                                                                                                                               ttAAGCGTGCCCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAACAGCAGTTGAAAGACATGGGGGAGGCG‑TTGGATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGAACTGGGAATGCTGGGAATG‑GACGGTCACTACGAAGCCAAAATGACGATGCATAACGCGGGAGTGATTTTAGCCggc                                                    >  1:682484/1‑249 (MQ=255)                                                                                                                                                                                                tAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGATGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTTCTCATTGATGGGGATGGGGGCGGTTCCGGCAAAGCATCGTCAGGCACTGGGCCTGCTGGGAATG‑GACGGTACCTACGAAGCCAATATGACGAAGCATAACGCGGATGTGGTTTTCggcggcg                                                   >  1:662764/1‑245 (MQ=255)                                                                                                                                                                                                  aGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCCGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCCTGCTGGGAATG‑CCCGGTACCTACGAAGCCAATATGGCGATGCATAACGCGGATGTGATTTTCGCCGTCggg                                                 >  1:242352/1‑251 (MQ=255)                                                                                                                                                                                                         tctGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTTCGGCAACGCATCGGCAGGCACTGGGCATGCTGGGAATG‑CACGGTAACTACGAAGCCAATATGACGATGCCTAACGCGGCTTTGATTTTCGCCGTCgggggtcgtt                                          >  1:163593/1‑245 (MQ=255)                                                                                                                                                                                                            gccaaCGCTGGCGGCGTCAAAAAAATCGGTTGGTTAAGAAGGTGGTGGGGCAACCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑GTGAATCTGCCCGTGGTTGCCTCATTGATGGGGCTGGGGGCGTTTCCGGCACCGCATCGTCAGGCACGGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGAtgtg                                       <  2:19721/248‑4 (MQ=255)                                                                                                                                                                                                                     ggtggCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGGACGCTTTGATGACCGa                               >  1:428038/5‑250 (MQ=255)                                                                                                                                                                                                                      gtggCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAac                             <  1:387582/248‑1 (MQ=255)                                                                                                                                                                                                                         cgatccaaaaacCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAGCAGTAGTTGAAAGAAACGGTGGAGGCG‑GTTAACCTGCCCGTTGTTTGCTCAGTGATGGGTCTGTGGGCGTTTCCGGCAACTCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTCCCTACGAAGCCAATATGACGATGCATAACGCTGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAacgac                          <  1:312540/245‑1 (MQ=255)                                                                                                                                                                                                                          cGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATATGATTTTCGCCGGCGGGGTTCGATTTGATGACCGAacgacg                         >  1:507298/1‑251 (MQ=255)                                                                                                                                                                                                                               aaaaaaCCGGTTGGCTACGGAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACaa                    <  2:428038/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTg                <  1:678704/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGAGGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTg                <  1:449626/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCaa            <  1:480726/251‑1 (MQ=255)                                                                                                                                                                                                                                           gtcgaCGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACTCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGATCAATCTGGCAAAGt         <  2:662764/246‑1 (MQ=255)                                                                                                                                                                                                                                           gTCTACGTAGGTGGTGGGGCAATCCCGGCGGGCCGCCATCAGCAGTTGAAAGAAAGGGTGTAGGCG‑TTGAACCTGCCCGTTGTTTCCTCATTGAGGTGGCTGGGGGCGTTTCCGGCAACGCATCGGCAGGCACTGTGCATGCTTGGAATG‑CTCGGGACCTCCGAAGCCAATATGACGATGCATAACGCGGATGTGATGTTCGCCGTCGGGGTACGATTTGAGGACCGAACGTCTATCAATCTGGCAAAGTa        <  2:99567/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCGTAGGTGGTGGGGTAATCACGGCTGGCTGCCAGCAGCAGTTGAAAGAAATGGTGGAGGCG‑TTGAAACGGCCCGGTGTTTCCTCAGTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGGCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGc    <  2:527141/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gTAGGTGGTGGGGCAATCACGGCGGGCTGACATCAGCAGTTGCAAGAAATGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCAATGGGCATGCTGGGAATG‑CACGGGACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGGTTTTCGCCGTCGGGGGACGATTTGATGACCGAACGACGAACAATCttgccacatactgccc  >  1:571943/1‑236 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        AAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCG‑TTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATG‑CACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCC  >  NC_000913/86026‑86516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑