Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 84,251 G→T intergenic (‑543/‑117) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091384,2510GT82.9% 73.2 / 8.5 35intergenic (‑543/‑117)leuL/leuOleu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (13/16);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

ATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTAC  >  NC_000913/84021‑84486
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
atcttgtgtatatTAATAATCTATTAATGTTAAAAATTAATGCATTTAATATATAAATTAAATATTAAATAAGCACAATTAAGCCATTTTGGTGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGAAACAAAAACATTATGTATTATTATGCTGTAGTAAATGACTCATTCCACGGCAATGGATTTTGTTTTTTTCAGAACCCGTATCTTTATGTTTGCCTAATTTTACTCCTTTTCCtt                                                                                                                                                                                                                          <  2:466509/241‑1 (MQ=255)
   tGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTGTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCttttt                                                                                                                                                                                                                       <  1:330392/250‑1 (MQ=255)
           attattGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAAGTTTACTCATTTTCCTTTTTCTTAtttg                                                                                                                                                                                                               <  2:34198/250‑2 (MQ=255)
             tattGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTtat                                                                                                                                                                                                             <  2:162930/250‑1 (MQ=255)
              attGATCAATTAATGTTAAGAATTAATGCATTAACTATATAAGTTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTTTGATTAGATTGTTTTCGAAACAAAAACATTATGGATTATTATGCTGTGGTAATTGACTCATTACACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTCCTCATTTTCATTTTTCTTATTTTatat                                                                                                                                                                                                           >  2:107703/1‑251 (MQ=255)
               ttGATCAATTATTGTTAAGGATTAATGCATTAAATATATATATTACTTATTAAATAAGCACTTTTAATCCATTTTGAAGATGATTATGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGTTGATTCTGCTGCGGGAAATGACTCATTCCACGGCTATGGATTCTGTTTTTATCATCACCAGTATCTTTATGTTCTCCTAATTTTACTAATTTTCCTTTTTCTTTTTTTatatt                                                                                                                                                                                                          >  2:168190/1‑250 (MQ=255)
                               aaGAATTAATGCATTAAATATATAAATTAATTAATAAATAAGCACAGTTAATCCATGTTGTAGATGATTGAGTAGTTGCGGTAGTTATGATTAGTTTGTTTTCGCAACAAAAACATTATGTATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGATCTTGTATCTTTATATTTTCCTAAATTGACTCATTTTTCAAGTTCTTATTTCATATTCATGATAAATCatat                                                                                                                                                                                           <  2:686961/250‑1 (MQ=255)
                                 gAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATAttct                                                                                                                                                                                        >  1:659547/1‑251 (MQ=255)
                                      aaTGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTACTTATTTTATATGCATGATAAAGCATAttctcgagg                                                                                                                                                                                   <  2:1266/251‑6 (MQ=255)
                                         gCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCAAGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATAttttcgaggttt                                                                                                                                                                                >  2:513564/1‑241 (MQ=255)
                                                           aattaTTAAATAAGCAAATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAACACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAAt                                                                                                                                                              >  1:585019/1‑251 (MQ=255)
                                                                  aaaTAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGAAGTTATGATTAGATTGTTTTCGCAACAACAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGAAATGGATTCTGTTTTTATCAGAACACGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTTTTTTATATTCATGATAAATCATATTCTTCAGGATTATTTCTCTGCTATCCATTaaggga                                                                                                                                                        >  2:829/1‑250 (MQ=255)
                                                                    aTAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAAACATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaaag                                                                                                                                                     <  2:308175/251‑1 (MQ=255)
                                                                     tAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATaagggaaagg                                                                                                                                                    >  2:210818/1‑251 (MQ=255)
                                                                       aGCACATTTAATCCATTTTGGAGATGATTGATTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaaaggga                                                                                                                                                  <  1:20635/251‑1 (MQ=255)
                                                                                            aGATGATTGAGTATTCGCTGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCTAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGt                                                                                                                              <  1:611936/250‑1 (MQ=255)
                                                                                                               gTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACaa                                                                                                          <  1:251925/251‑1 (MQ=255)
                                                                                                               gTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGGGTGACAGTGGAGTTAAGTATGCCAGAGGAACa                                                                                                           >  2:640355/1‑250 (MQ=255)
                                                                                                                     aTGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGat                                                                                                    <  1:552573/251‑1 (MQ=255)
                                                                                                                                   ttCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCAGTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                       <  2:228390/250‑1 (MQ=255)
                                                                                                                                               aCATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTATTCTCAGAACACGAATCTTTATGTTTTCCTACTTTTACTCATTTTCCTTTTTCTTCTTTTATATGCATGATAAATCATATTCTCGAGGATTCTTTCTCTGCATTCCAATAAGGGAAAGGGTGTTAAGTGTGACAGAGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCa                                                                           >  1:31609/1‑250 (MQ=255)
                                                                                                                                                cATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCa                                                                           >  1:238776/1‑249 (MQ=255)
                                                                                                                                                 cttATGGATTATTCTGCTGTGGTAAATGCCTCATTCCCCGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTAATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCTTTCAAATAAGGGACAGGGAGTTAAGTGTGACAGTGGAGtt                                                                                                                             >  2:312855/2‑198 (MQ=255)
                                                                                                                                                 aTTATGGATTATTATGCTGTGGTAAATTACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATGTTATATGCATGATAAACCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGtt                                                                                                                             <  1:312855/198‑1 (MQ=255)
                                                                                                                                                         ttattaTGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCt                                                                 >  2:286420/1‑250 (MQ=255)
                                                                                                                                                                gCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATg                                                          <  1:210818/250‑1 (MQ=255)
                                                                                                                                                                        aaaTGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCAACAGCTACGCATGGTCGAtctc                                                 >  1:406868/1‑251 (MQ=255)
                                                                                                                                                                                          cAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTc                               <  1:16871/251‑1 (MQ=255)
                                                                                                                                                                                                       ttttATCAGAACACGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGAGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGACGATCTAAACTTATTAACCGTTTTCGATGCCGAGAttc                  >  2:519342/1‑249 (MQ=255)
                                                                                                                                                                                                         ttaacataaCCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTTCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCa                 <  2:383971/243‑1 (MQ=255)
                                                                                                                                                                                                         ttATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATTGGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGATTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCa                 <  2:521733/250‑1 (MQ=255)
                                                                                                                                                                                                               gAACCCGTATCTTTATGTTTTCCTAATTTTACTCCTTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCAACAGCTACGCATGTTCGAt                                                    >  2:446049/1‑209 (MQ=255)
                                                                                                                                                                                                               gAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGAt                                                    <  1:446049/209‑1 (MQ=255)
                                                                                                                                                                                                                       aTCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGCTAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGGGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGCTCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAATACATTAc  >  1:173403/1‑251 (MQ=255)
                                                                                                                                                                                                                       aTCTTTATGTTTTCCTAATTTTACGCATTTTCCTTTTTCTTATTTTAGATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCAGTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCTCAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTa   <  1:107703/250‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
ATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTAC  >  NC_000913/84021‑84486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: