Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,520	C→A	F300L (TTC→TTA)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,520	0	C	A	94.4%	107.5 / ‑5.6	37	F300L (TTC→TTA)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (11/23);  minor base T (2/0);  total (14/23)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGT  >  NC_000913/78275‑78762                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    aTGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTTGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTAtttca                                                                                                                                                                                                                                                <  1:396239/251‑3 (MQ=255)     tATTTGTCGCCTCGACGTTAATGTGGTCCTGCAACACCATGTACATTATTGATATGTCGTTGTGGATCATTAGCGAGTTAGGATTGCCAGACAAACGGGCGGGTTTCCTGATGGGGGCGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGGGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATGTTATTTCATAg                                                                                                                                                                                                                                             <  1:185387/250‑1 (MQ=255)     tATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAg                                                                                                                                                                                                                                             <  2:227862/250‑1 (MQ=255)              ccTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCg                                                                                                                                                                                                                                   <  1:120850/251‑1 (MQ=255)                                      acacCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAAc                                                                                                                                                                                                          <  1:500516/251‑1 (MQ=255)                                                      tGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGc                                                                                                                                                                                           <  1:11098/250‑1 (MQ=255)                                                       gATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGGTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGGTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGGGGC‑CGGG‑GGACCG‑TTTTTTACCGGGGTGTTTTTTTTTTATATCCGTCTGGCGTTGTTGGCGCTGCACC‑ttttttttgatgtttttttctggct                                                                                                                                                                                         >  2:142713/1‑231 (MQ=255)                                                        atatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGCGTTCCTTATGGGGACGGCAGCTGGTATTGTAACACCCGCATTGTTTCTGGCTAGCTCATTTGTCAAAAGTTATGGGCAGCGGCGAATGGTGGTTATTGCCGTGGCGGCCAGG‑‑GGACTG‑TTTTATACCCGATTTTTTTTATTTTTTAACCCTCCGGCGTTGATGGAGCTGCAACCTTTTTAAC‑CCGTATTTTTCGGcctt                                                                                                                                                                                        >  1:689017/1‑248 (MQ=255)                                                        aaatggcggtttggaataGTAGCGAGTTAGGATTGCCATGCAAACTGGTCGGTTTCCTGATGGGGTCGGCAGGTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCCAACGTTATGGTAAGCGGAGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GGACTG‑TTTTACCCCGTATTGATTTTATTTCATAGCCGTCTGGCGTTGAGGACGCGGCAAC‑TTGTTAACTCTGTATGTATCGGCAt                                                                                                                                                                                         <  2:668647/233‑1 (MQ=255)                                                         tatGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCCGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAAAGTTATGGTAAGCGGCGAATGATGGTCATAGCCGTGGCGGC‑AGGA‑GTAATG‑GTTTACACCGGGTTGATTTTATTTCATAGCCGGCTGGCGTTGATGACGCTGCAAC‑TTTTTAAAGCTGTATTTATTGGCAttg                                                                                                                                                                                       >  1:530143/1‑251 (MQ=255)                                                                     gatccgtcgcgaggtgggATTGCCAGCCAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCGGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGTTAGGCGGCGAATGATGGTCATATCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATGGCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TGTTTAACGCTGTATTTATCGTCATTGTTGCGGGTATTg                                                                                                                                                                           <  2:638322/235‑1 (MQ=255)                                                                        cAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGGCATAGCAGGGGCGGC‑CGGA‑GGACTG‑TTTTACAACGGATTGATTTTTTTTCCTAGCCGTCTGGCGTTGATGAAGCGGCAAC‑TTTTTTAAGATGTATTTATCGGGATTGTTGCggggtttgggg                                                                                                                                                                        >  1:530821/1‑243 (MQ=255)                                                                               gAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATg                                                                                                                                                                 <  1:70197/251‑1 (MQ=255)                                                                                           ccAGACAAACTGGCGGGGTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCCGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGGATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTGGTTTTCCAC‑GGATTTGTTTTATTTCATAGCCGTCTGGGGTTGGTGACGATGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGGATTTGGGTGCTATGGTTTTAGGAttt                                                                                                                                                     >  1:632108/1‑251 (MQ=255)                                                                                                      ggCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGa                                                                                                                                           <  1:142713/250‑1 (MQ=255)                                                                                                              tCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCggg                                                                                                                                   <  1:372415/250‑1 (MQ=255)                                                                                                                     ggggACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCt                                                                                                                            >  1:244571/1‑250 (MQ=255)                                                                                                                          cGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTaccacc                                                                                                                      <  2:244571/251‑1 (MQ=255)                                                                                                                              aGCTGGTCTGGAAATACCCGCACTGATTCTGTCTGGCTACTATGTCACACGTTATGGTACGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGGTTTTGTTTCCTAGCCGTCTGGCGTTGATGACGCTGCAAA‑TTTTTTACGCTTTTTTTATCGGCATTGTTGCGGGTATTGGGATGCTTTGGTTTCAGGTTTTAATTCCTGGAAGAGCGGGGGCAGCCACCACCttct                                                                                                                  >  2:650345/1‑249 (MQ=255)                                                                                                                                              ccAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATg                                                                                                                                                                 >  1:154266/1‑188 (MQ=255)                                                                                                                                              ccAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATg                                                                                                                                                                 <  2:154266/188‑1 (MQ=255)                                                                                                                                                                    cctaTGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGGCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGGGCGGGGGCAGCTACCACCTTATTTACTCACAGTATTTATAACGGGGGAATTctggctggg                                                                            >  2:254903/2‑250 (MQ=255)                                                                                                                                                                          tCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCCGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTAtt                                                                      >  1:463164/1‑251 (MQ=255)                                                                                                                                                                              aCGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTa                                                                                                                                                    <  1:542167/169‑1 (MQ=255)                                                                                                                                                                              aCGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCGGGCGTTGATGACGCTGCAAC‑TTTTTAAAGCTGTATTTATCGGCATTGTTGCGGGGATTGGGTTGCTATGGTTTCATGATTTa                                                                                                                                                    >  2:542167/1‑169 (MQ=255)                                                                                                                                                                                 ttATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAggg                                                                 >  1:455920/1‑249 (MQ=255)                                                                                                                                                                                        aaGCGTCGAATGAGGATCATAGCAGTGGCGGC‑AGGA‑GTACGG‑TTTTGCACCGTAGTGAGTTTATTTCATAGCCGTGAGGCGTTGATGACGCTGCAGC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGTGATTGGGATGCTATGGTTTCATGAGTTAATGCCTGGAAGAGCGGGGGCAGCGACCACCTTATTGACTAACAGTATGTCTACCGGGGTAAGTCTGGCTGGCGTTATTCAGGGAGCAATTGc                                                        <  2:632116/251‑1 (MQ=255)                                                                                                                                                                                        aaGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGc                                                        <  2:632108/251‑1 (MQ=255)                                                                                                                                                                                             gcgAATGATGGTCATAGCAGTGGCTGC‑AGGA‑GTACTG‑TTTTTCACCGGATTGATTTTATTTCATAGCCGTCTGGCGGTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCATGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACaaa                                                   <  2:612309/251‑1 (MQ=255)                                                                                                                                                                                                         cATAGCAGGGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGGTTGATTTTATTTCATAGGCGTCGGGCGGTGATGACGCGGCAAC‑GTTTTAACGCTGTATTTAGCGGCATTGTTGCGGGTAGTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTAGCACCTTATTTGCTAACAGGATTTCTACCGGGGTAAGTCTGGCTGGCGTTAGTCAGGGAGCAATTGCACAAAGGTGGGGGcgct                                       <  2:68166/251‑4 (MQ=255)                                                                                                                                                                                                                  ggcggc‑AGGATGT‑CTG‑GTTTACTCCGGATTGAGTTGATTTCATAGCCGTCTGGCTTTGATGACTCTGCAAC‑TTTTTAACGCTGTTTTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGGGCGGGGGCAGCTACCACCTTATTTTCTAACAGTATTTCTACCGGGGTAATTCTGGCTGTCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTc                               <  2:643339/250‑1 (MQ=255)                                                                                                                                                                                                                       c‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTgg                         >  1:570446/1‑251 (MQ=255)                                                                                                                                                                                                                         gcaggaatactttgtttccccGGATTGTTTTTATTTCATAGCTGTCTGGCGTTGAGTACGCTTCAAC‑TTTTTAACGCTGTATTTATCGTCTTTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGTCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGTGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACATTGCTGTCTACTg                          <  2:571465/232‑1 (MQ=255)                                                                                                                                                                                                                                      tttACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGtt             >  1:512694/1‑251 (MQ=255)                                                                                                                                                                                                                                        tACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTa            <  1:254903/250‑1 (MQ=255)                                                                                                                                                                                                                                               gattgattTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGTTGCACTTTGCTGTCTACTTGGTAATTGCTGGTATTGCtgtt    <  2:665113/251‑1 (MQ=255)                                                                                                                                                                                                                                                  tgattTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgttat  <  1:533641/250‑3 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    ATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGC‑AGGA‑GTACTG‑TTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAAC‑TTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGT  >  NC_000913/78275‑78762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑