Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,364 G→A A312A (GCC→GCT)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,3640GA93.8% 181.5 / 0.8 66A312A (GCC→GCT) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (34/27);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

TTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGG  >  NC_000913/76120‑76610
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
tgaaggTGTGGGCATGGTGCCAACGGGGGAGCTGTCCATAAGTCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCGTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCagctgg                                                                                                                                                                                                                                                   <  2:205906/246‑1 (MQ=255)
     gggCGGGCAGGGTGCCCACGGGGGAGCAGTCCAGAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCtcgtc                                                                                                                                                                                                                                             <  2:58436/249‑1 (MQ=255)
          ggCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTACTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCa                                                                                                                                                                                                                                         >  1:95371/1‑250 (MQ=255)
                        gggggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAcc                                                                                                                                                                                                                          >  1:401655/1‑251 (MQ=255)
                            gAGCAGCCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCATTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAGTCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGa                                                                                                                                                                                                                      <  1:272789/251‑1 (MQ=255)
                                 ggccATAACCTGGGAACCACAGTTGCTGGTTCGGTTCCTCATCGAAATAGTCCAGGTTAGTTGTAGAAAGCACATCGCTTTCCCAGTCCTTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATATTAATTAGTAGCACCCTGCCTCCAGGCGGCTTGCAATCCCTTCTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCGCCAGCTGTCTCGGCGGCAATTTCCGGCAGAACCCatccgtt                                                                                                                                                                                                                  <  2:397623/248‑7 (MQ=255)
                                     aTAAGCCGGGAACCAAAGTTGCTGGTACTGTTCCTTAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACtt                                                                                                                                                                                                             >  1:553495/1‑251 (MQ=255)
                                       aaGCCGGGATCCACAGTTGCTGGTACTGTGCCTCAGCGAAATAGTTCAGATTAGTTGGAGAAAGCTCATAGCTTACCCAGGCCATGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCGTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCCGAACCCAGACGTTAACTtc                                                                                                                                                                                                            <  2:26759/250‑1 (MQ=255)
                                        aGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGTACCTTTTAGCATCAGACCTCCAGCTGGCTCTTCGGCAATTTCCGGCAGAACCCAGACGTTAACTtcgt                                                                                                                                                                                                          <  1:605551/251‑1 (MQ=255)
                                        aGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATACTAAATCGTAGCAACCTTCCTCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCATCTGGCTCGTCGGCAATTTCCGGCCGAACCCAGACGTTCACTtcgt                                                                                                                                                                                                          >  2:74159/1‑251 (MQ=255)
                                          ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGGCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCGCCAGCTGGCTCGTCGGCAATTTCCGTCAGAACCCAGACGTTAACTtcgtcg                                                                                                                                                                                                        <  1:485387/251‑1 (MQ=255)
                                           cGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATGAGTTGTAGAACTCACATAGCTTACCCAGTCCCTGACTTGCTGATTGGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCATCCAGGCGGCTTGCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCAGCAAGTACCGGCAGAACCCAGACGTTAACTtcgtcg                                                                                                                                                                                                        >  1:160190/1‑250 (MQ=255)
                                               aaCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACACAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTACATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGAAATTTCCGGCAGCACCCAGACGTTAACTTCGGCGATTa                                                                                                                                                                                                    >  1:396801/1‑250 (MQ=255)
                                                   acaGTTGCTGGTACTGTTCCTCAGCGAACTAGAACAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGGAGCAACCTTCCTCCAGGCGGCTTTTCATCTCTTTTTCCACACCCTGTGGACCTTTTCGCCTCAGCCCTCCGGCTGTCTCGTCGGCAATTTCCGGCAGAAACCAGAAGTTACTTTCGTCGATTAATGcc                                                                                                                                                                                               >  2:574773/1‑251 (MQ=255)
                                                   acaGTGGCTGGAAATGTGCCGCAGCGAAAGAGACCAGATTAGTGGGAGAAAGCACATAGCTGACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTGCCTCCAGGCGGCTTTCAATCTCTTTTGCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTGCGTCGATTAATGcc                                                                                                                                                                                               <  2:340032/251‑1 (MQ=255)
                                                    caGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGccc                                                                                                                                                                                              >  1:3113/1‑251 (MQ=255)
                                                         gCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTa                                                                                                                                                                                          <  1:56521/250‑1 (MQ=255)
                                                           tGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGAAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTACTCACCCTGTGGACCTTTTAGCATCAGACCTCCCGCTGGCTCGTCGGCCATTTCCGGCAGAACCCAGACGTTCACTTCGTCGATTAATGCCCGGAAAcc                                                                                                                                                                                       >  2:674140/1‑251 (MQ=255)
                                                            gtgaccgTTCCTCAGCGAAATATACCAGATTAGTTGGAGAAAACAAATATCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTaa                                                                                                                                                                                         <  2:702439/242‑1 (MQ=255)
                                                            ggTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGGAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAAcc                                                                                                                                                                                       <  1:90206/250‑1 (MQ=255)
                                                            ggTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAAcc                                                                                                                                                                                       <  1:432541/250‑1 (MQ=255)
                                                             gTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCg                                                                                                                                                                                      >  1:280880/1‑250 (MQ=255)
                                                                         aGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCgaag                                                                                                                                                                                   >  1:294234/1‑241 (MQ=255)
                                                                         aGCGAAATAGACCAGATTAGGTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCgaag                                                                                                                                                                                   <  2:294234/241‑1 (MQ=255)
                                                                           cGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAAt                                                                                                                                                                       >  1:190012/1‑251 (MQ=255)
                                                                                      aGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAtttt                                                                                                                                                            >  1:664257/1‑251 (MQ=255)
                                                                                      aGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGAAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTAATCGAATGCCTGAAtttt                                                                                                                                                            >  1:664223/1‑251 (MQ=255)
                                                                                          tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGt                                                                                                                                                        >  1:277891/1‑251 (MQ=255)
                                                                                          tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCGGAGTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTGCCTCCAGGCGGCTTTCAATCTCTTTTTCCGCCCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAg                                                                                                                                                         <  2:703806/250‑1 (MQ=255)
                                                                                               ggAGAAAGCACGTAGCGTACGCAGGCCATGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCATCCTTCCGACTGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCATCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACGTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGAtt                                                                                                                                                   <  2:125343/251‑1 (MQ=255)
                                                                                                gagaAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGCATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTAAGTTGATTg                                                                                                                                                  >  2:350652/1‑251 (MQ=255)
                                                                                                    aaGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCCACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCCGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAACCAGACGTTAACTTCGTCGCTTAATGCCCGGTAAACGCAGAAGTCATCGAATGCCTGCATTTTCAGTTGATTGGTgg                                                                                                                                              >  2:292161/1‑250 (MQ=255)
                                                                                                               ttACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATc                                                                                                                                   >  1:89370/1‑251 (MQ=255)
                                                                                                               ttACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGGTTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTCTCAGTTGATTGGGGCTGTTGCGAATc                                                                                                                                   >  1:206850/1‑251 (MQ=255)
                                                                                                                tacccgggcccTTACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGGCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCGGTTGCGGATc                                                                                                                                   <  2:121123/242‑1 (MQ=255)
                                                                                                                 aCCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTGTCAGTTGATTGGTGCTGTTGCGGATCa                                                                                                                                  <  1:265065/250‑1 (MQ=255)
                                                                                                                 aCCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTGTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCATACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCAc                                                                                                                                 <  1:297148/251‑1 (MQ=255)
                                                                                                                 aCCCAGTCCCTGACTTGATGATTCGCCCCGCGATGGGAGCGGCTGTCGACTAGTACATAGTAGCACCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTGACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCCTCGAATGCCTGAATTTTCCGTTGGATGGTGCTGTTGCggttccc                                                                                                                                 >  2:297148/1‑245 (MQ=255)
                                                                                                                  cccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATCGGTGCTGTTGc                                                                                                                                        >  2:706415/1‑243 (MQ=255)
                                                                                                                  cccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATCGGTGCTGTTGc                                                                                                                                        <  1:706415/243‑1 (MQ=255)
                                                                                                                   ccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGACCTCCAGCTGGCTCGTCGGCCATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTTGTGCGGATCACCg                                                                                                                               >  2:377670/1‑251 (MQ=255)
                                                                                                                            gACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGAc                                                                                                                      <  1:263731/251‑1 (MQ=255)
                                                                                                                                 gCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACTTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTGGAGTGGTGCTGTTGCGGATCACCGCATTCGGACCGGt                                                                                                                  <  2:673069/250‑1 (MQ=255)
                                                                                                                                    gATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGcc                                                                                                               <  1:347638/250‑1 (MQ=255)
                                                                                                                                           cccGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGCCCGGTGCCgatgggat                                                                                                       >  1:671355/1‑251 (MQ=255)
                                                                                                                                                gATGGGTGCGGATGTCGTATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCATCACCATGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACTTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATTCCTGAATTTTACGTTGCTTGGAGCTGTTGCGGATAACCGCATACGGCCCGGTGCCGGTGGGAtggctg                                                                                                  >  2:690921/1‑251 (MQ=255)
                                                                                                                                                gATGGGGGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACACTGTGGACCTTTTAGCATCAGACCTACAGCTGGCTCGTCGGCAATTTCCGGCCGCACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGACGAAGTCATCGAAGTCCTGAATTTTCCGTTGATTGGTGCTGTTGCGGATCACCGCATCCGGACCGGGGCCGATGGGAtggctg                                                                                                  >  2:518530/1‑251 (MQ=255)
                                                                                                                                                 aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGAGTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGAtggctgg                                                                                                 <  1:448855/251‑1 (MQ=255)
                                                                                                                                                            tGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCGAAGTTACTg                                                                                      >  1:192633/1‑251 (MQ=255)
                                                                                                                                                                gaataGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTACTCACCCTGGGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCAAGACGTTAACTTCGTCGATTAATGCCCGGTAACCAAAGAAGTCATCGAATGCCTGAATTTTCAGTTGGTTGGTGCTGTTGCGGCTCACCGCATACGGACCGGGGCCGATGGGATGGCTGGCAAAGTTACTGAggg                                                                                  >  1:617874/5‑251 (MQ=255)
                                                                                                                                                                  ctaggaaaTAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGCCCGGTGCCGATGGGATGGCTGGCAAAGGTACTGAGGGtt                                                                                >  2:65326/6‑251 (MQ=255)
                                                                                                                                                                  ataGTAAATAGTAGCAACCTTCCTCCATGCGGCTTTCAATCTCTTTTTCCGCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGAGGGTATGGCTGGCAAAGTTACTGAGGGtt                                                                                <  2:407483/249‑1 (MQ=255)
                                                                                                                                                                  ataGTAAAGAGTAGCATCCTTCCTCCAGGCGGCTTTAAATCTCTTTGTCCTCACCCTTTGGACCTTTTAGCAGCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAAACCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTTCTGTTGCGGATCACCGCATACGGACCGGTGCCGTTGGGATGGCTGGCAAAGTTTCTGAGGGtt                                                                                <  2:701329/249‑1 (MQ=255)
                                                                                                                                                                         acagtagcaatctgtcccccggcggcTGTCAATCTCTTTTTCCGCGCCCTGTGGACTGTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTCTCAGTTGATTGGTGCTGTTGCGAAGCACCGCAGACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCAtt                                                                         <  2:206850/231‑1 (MQ=255)
                                                                                                                                                                                  aCCTTCCTCAAGGCGGCTTTCAATCTCTTTTTACTTACCCTGCGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGATGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTTCAGATGGGGTGGCTGGCAAAGTTACTGAGGGGTTCCAATTCGCGCGGc                                                                 >  2:237477/1‑250 (MQ=255)
                                                                                                                                                                                    cTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAgg                                                              >  1:94167/1‑251 (MQ=255)
                                                                                                                                                                                         cccAGGCGGCTTTCAATCTCTGTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTAGGCAAGTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGatcat                                                         <  2:3113/250‑1 (MQ=255)
                                                                                                                                                                                           cAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGGAGGATCATCg                                                       <  2:89370/251‑1 (MQ=255)
                                                                                                                                                                                           cAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCg                                                       >  2:304798/1‑251 (MQ=255)
                                                                                                                                                                                                  cTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAAc                                                >  1:75424/1‑251 (MQ=255)
                                                                                                                                                                                                   tttCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACt                                               >  1:315485/1‑251 (MQ=255)
                                                                                                                                                                                                                ttCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGACTGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGCACTAGCCCcagcaat                                   >  1:660955/1‑248 (MQ=255)
                                                                                                                                                                                                                 tCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAAATTCGTCGTTTAATGCACGGTAACCGAAGAAGTCACCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATCCGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCAACGCCgtcaattgacccagcaata                                  >  2:405094/1‑232 (MQ=255)
                                                                                                                                                                                                                          tgtgGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATAGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCAAACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTT‑ACCATTCGCGCGGCAGGATCATCGCCGGAACTTTCCCCAGCAGTATCGGTAAcc                         >  2:208700/1‑249 (MQ=255)
                                                                                                                                                                                                                             ggACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCg                     <  1:377670/251‑1 (MQ=255)
                                                                                                                                                                                                                                          tCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCACAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTACCCCAGCAATAACGGTAACCAGCGGGCCGGTTGCGTg        >  2:677573/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATgg  >  2:199966/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
TTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGG  >  NC_000913/76120‑76610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑