Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,358 C→A G314G (GGG→GGT)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,3580CA94.1% 181.9 / ‑2.3 68G314G (GGG→GGT) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (35/29);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTG  >  NC_000913/76114‑76604
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
cGCTCTTGATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGGTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACGTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCGCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTGTAGCATCAGACCTcca                                                                                                                                                                                                                                                  <  2:703276/251‑2 (MQ=255)
      ttATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTAAAAAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTATTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTccacatggc                                                                                                                                                                                                                                            >  2:196549/1‑244 (MQ=255)
      tgaaggTGTGGGCATGGTGCCAACGGGGGAGCTGTCCATAAGTCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCGTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCagctgg                                                                                                                                                                                                                                             <  2:205906/246‑1 (MQ=255)
           gggCGGGCAGGGTGCCCACGGGGGAGCAGTCCAGAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCtcgtc                                                                                                                                                                                                                                       <  2:58436/249‑1 (MQ=255)
                ggCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTACTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCa                                                                                                                                                                                                                                   >  1:95371/1‑250 (MQ=255)
                              gggggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAcc                                                                                                                                                                                                                    >  1:401655/1‑251 (MQ=255)
                                  gAGCAGCCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCATTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAGTCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGa                                                                                                                                                                                                                <  1:272789/251‑1 (MQ=255)
                                       ggccATAACCTGGGAACCACAGTTGCTGGTTCGGTTCCTCATCGAAATAGTCCAGGTTAGTTGTAGAAAGCACATCGCTTTCCCAGTCCTTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATATTAATTAGTAGCACCCTGCCTCCAGGCGGCTTGCAATCCCTTCTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCGCCAGCTGTCTCGGCGGCAATTTCCGGCAGAACCCatccgtt                                                                                                                                                                                                            <  2:397623/248‑7 (MQ=255)
                                           aTAAGCCGGGAACCAAAGTTGCTGGTACTGTTCCTTAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACtt                                                                                                                                                                                                       >  1:553495/1‑251 (MQ=255)
                                             aaGCCGGGATCCACAGTTGCTGGTACTGTGCCTCAGCGAAATAGTTCAGATTAGTTGGAGAAAGCTCATAGCTTACCCAGGCCATGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCGTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCCGAACCCAGACGTTAACTtc                                                                                                                                                                                                      <  2:26759/250‑1 (MQ=255)
                                              aGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGTACCTTTTAGCATCAGACCTCCAGCTGGCTCTTCGGCAATTTCCGGCAGAACCCAGACGTTAACTtcgt                                                                                                                                                                                                    <  1:605551/251‑1 (MQ=255)
                                              aGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATACTAAATCGTAGCAACCTTCCTCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCATCTGGCTCGTCGGCAATTTCCGGCCGAACCCAGACGTTCACTtcgt                                                                                                                                                                                                    >  2:74159/1‑251 (MQ=255)
                                                ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGGCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCGCCAGCTGGCTCGTCGGCAATTTCCGTCAGAACCCAGACGTTAACTtcgtcg                                                                                                                                                                                                  <  1:485387/251‑1 (MQ=255)
                                                 cGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATGAGTTGTAGAACTCACATAGCTTACCCAGTCCCTGACTTGCTGATTGGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCATCCAGGCGGCTTGCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCAGCAAGTACCGGCAGAACCCAGACGTTAACTtcgtcg                                                                                                                                                                                                  >  1:160190/1‑250 (MQ=255)
                                                     aaCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACACAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTACATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGAAATTTCCGGCAGCACCCAGACGTTAACTTCGGCGATTa                                                                                                                                                                                              >  1:396801/1‑250 (MQ=255)
                                                         acaGTTGCTGGTACTGTTCCTCAGCGAACTAGAACAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGGAGCAACCTTCCTCCAGGCGGCTTTTCATCTCTTTTTCCACACCCTGTGGACCTTTTCGCCTCAGCCCTCCGGCTGTCTCGTCGGCAATTTCCGGCAGAAACCAGAAGTTACTTTCGTCGATTAATGcc                                                                                                                                                                                         >  2:574773/1‑251 (MQ=255)
                                                         acaGTGGCTGGAAATGTGCCGCAGCGAAAGAGACCAGATTAGTGGGAGAAAGCACATAGCTGACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTGCCTCCAGGCGGCTTTCAATCTCTTTTGCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTGCGTCGATTAATGcc                                                                                                                                                                                         <  2:340032/251‑1 (MQ=255)
                                                          caGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGccc                                                                                                                                                                                        >  1:3113/1‑251 (MQ=255)
                                                               gCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTa                                                                                                                                                                                    <  1:56521/250‑1 (MQ=255)
                                                                 tGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGAAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTACTCACCCTGTGGACCTTTTAGCATCAGACCTCCCGCTGGCTCGTCGGCCATTTCCGGCAGAACCCAGACGTTCACTTCGTCGATTAATGCCCGGAAAcc                                                                                                                                                                                 >  2:674140/1‑251 (MQ=255)
                                                                  gtgaccgTTCCTCAGCGAAATATACCAGATTAGTTGGAGAAAACAAATATCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTaa                                                                                                                                                                                   <  2:702439/242‑1 (MQ=255)
                                                                  ggTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGGAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAAcc                                                                                                                                                                                 <  1:90206/250‑1 (MQ=255)
                                                                  ggTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAAcc                                                                                                                                                                                 <  1:432541/250‑1 (MQ=255)
                                                                   gTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCg                                                                                                                                                                                >  1:280880/1‑250 (MQ=255)
                                                                               aGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCgaag                                                                                                                                                                             >  1:294234/1‑241 (MQ=255)
                                                                               aGCGAAATAGACCAGATTAGGTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCgaag                                                                                                                                                                             <  2:294234/241‑1 (MQ=255)
                                                                                 cGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAAt                                                                                                                                                                 >  1:190012/1‑251 (MQ=255)
                                                                                            aGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAtttt                                                                                                                                                      >  1:664257/1‑251 (MQ=255)
                                                                                            aGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGAAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTAATCGAATGCCTGAAtttt                                                                                                                                                      >  1:664223/1‑251 (MQ=255)
                                                                                                tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGt                                                                                                                                                  >  1:277891/1‑251 (MQ=255)
                                                                                                tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCGGAGTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTGCCTCCAGGCGGCTTTCAATCTCTTTTTCCGCCCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAg                                                                                                                                                   <  2:703806/250‑1 (MQ=255)
                                                                                                     ggAGAAAGCACGTAGCGTACGCAGGCCATGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCATCCTTCCGACTGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCATCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACGTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGAtt                                                                                                                                             <  2:125343/251‑1 (MQ=255)
                                                                                                      gagaAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGCATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTAAGTTGATTg                                                                                                                                            >  2:350652/1‑251 (MQ=255)
                                                                                                          aaGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCCACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCCGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAACCAGACGTTAACTTCGTCGCTTAATGCCCGGTAAACGCAGAAGTCATCGAATGCCTGCATTTTCAGTTGATTGGTgg                                                                                                                                        >  2:292161/1‑250 (MQ=255)
                                                                                                                     ttACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATc                                                                                                                             >  1:89370/1‑251 (MQ=255)
                                                                                                                     ttACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGGTTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTCTCAGTTGATTGGGGCTGTTGCGAATc                                                                                                                             >  1:206850/1‑251 (MQ=255)
                                                                                                                      tacccgggcccTTACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGGCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCGGTTGCGGATc                                                                                                                             <  2:121123/242‑1 (MQ=255)
                                                                                                                       aCCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTGTCAGTTGATTGGTGCTGTTGCGGATCa                                                                                                                            <  1:265065/250‑1 (MQ=255)
                                                                                                                       aCCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTGTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCATACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCAc                                                                                                                           <  1:297148/251‑1 (MQ=255)
                                                                                                                       aCCCAGTCCCTGACTTGATGATTCGCCCCGCGATGGGAGCGGCTGTCGACTAGTACATAGTAGCACCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTGACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCCTCGAATGCCTGAATTTTCCGTTGGATGGTGCTGTTGCggttccc                                                                                                                           >  2:297148/1‑245 (MQ=255)
                                                                                                                        cccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATCGGTGCTGTTGc                                                                                                                                  >  2:706415/1‑243 (MQ=255)
                                                                                                                        cccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATCGGTGCTGTTGc                                                                                                                                  <  1:706415/243‑1 (MQ=255)
                                                                                                                         ccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGACCTCCAGCTGGCTCGTCGGCCATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTTGTGCGGATCACCg                                                                                                                         >  2:377670/1‑251 (MQ=255)
                                                                                                                                  gACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGAc                                                                                                                <  1:263731/251‑1 (MQ=255)
                                                                                                                                       gCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACTTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTGGAGTGGTGCTGTTGCGGATCACCGCATTCGGACCGGt                                                                                                            <  2:673069/250‑1 (MQ=255)
                                                                                                                                          gATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGcc                                                                                                         <  1:347638/250‑1 (MQ=255)
                                                                                                                                                 cccGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGCCCGGTGCCgatgggat                                                                                                 >  1:671355/1‑251 (MQ=255)
                                                                                                                                                      gATGGGTGCGGATGTCGTATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCATCACCATGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACTTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATTCCTGAATTTTACGTTGCTTGGAGCTGTTGCGGATAACCGCATACGGCCCGGTGCCGGTGGGAtggctg                                                                                            >  2:690921/1‑251 (MQ=255)
                                                                                                                                                      gATGGGGGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACACTGTGGACCTTTTAGCATCAGACCTACAGCTGGCTCGTCGGCAATTTCCGGCCGCACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGACGAAGTCATCGAAGTCCTGAATTTTCCGTTGATTGGTGCTGTTGCGGATCACCGCATCCGGACCGGGGCCGATGGGAtggctg                                                                                            >  2:518530/1‑251 (MQ=255)
                                                                                                                                                       aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGAGTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGAtggctgg                                                                                           <  1:448855/251‑1 (MQ=255)
                                                                                                                                                                  tGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCGAAGTTACTg                                                                                >  1:192633/1‑251 (MQ=255)
                                                                                                                                                                      gaataGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTACTCACCCTGGGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCAAGACGTTAACTTCGTCGATTAATGCCCGGTAACCAAAGAAGTCATCGAATGCCTGAATTTTCAGTTGGTTGGTGCTGTTGCGGCTCACCGCATACGGACCGGGGCCGATGGGATGGCTGGCAAAGTTACTGAggg                                                                            >  1:617874/5‑251 (MQ=255)
                                                                                                                                                                        ctaggaaaTAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGCCCGGTGCCGATGGGATGGCTGGCAAAGGTACTGAGGGtt                                                                          >  2:65326/6‑251 (MQ=255)
                                                                                                                                                                        ataGTAAATAGTAGCAACCTTCCTCCATGCGGCTTTCAATCTCTTTTTCCGCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGAGGGTATGGCTGGCAAAGTTACTGAGGGtt                                                                          <  2:407483/249‑1 (MQ=255)
                                                                                                                                                                        ataGTAAAGAGTAGCATCCTTCCTCCAGGCGGCTTTAAATCTCTTTGTCCTCACCCTTTGGACCTTTTAGCAGCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAAACCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTTCTGTTGCGGATCACCGCATACGGACCGGTGCCGTTGGGATGGCTGGCAAAGTTTCTGAGGGtt                                                                          <  2:701329/249‑1 (MQ=255)
                                                                                                                                                                               acagtagcaatctgtcccccggcggcTGTCAATCTCTTTTTCCGCGCCCTGTGGACTGTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTCTCAGTTGATTGGTGCTGTTGCGAAGCACCGCAGACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCAtt                                                                   <  2:206850/231‑1 (MQ=255)
                                                                                                                                                                                        aCCTTCCTCAAGGCGGCTTTCAATCTCTTTTTACTTACCCTGCGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGATGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTTCAGATGGGGTGGCTGGCAAAGTTACTGAGGGGTTCCAATTCGCGCGGc                                                           >  2:237477/1‑250 (MQ=255)
                                                                                                                                                                                          cTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAgg                                                        >  1:94167/1‑251 (MQ=255)
                                                                                                                                                                                               cccAGGCGGCTTTCAATCTCTGTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTAGGCAAGTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGatcat                                                   <  2:3113/250‑1 (MQ=255)
                                                                                                                                                                                                 cAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGGAGGATCATCg                                                 <  2:89370/251‑1 (MQ=255)
                                                                                                                                                                                                 cAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCg                                                 >  2:304798/1‑251 (MQ=255)
                                                                                                                                                                                                        cTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAAc                                          >  1:75424/1‑251 (MQ=255)
                                                                                                                                                                                                         tttCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACt                                         >  1:315485/1‑251 (MQ=255)
                                                                                                                                                                                                                      ttCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGACTGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGCACTAGCCCcagcaat                             >  1:660955/1‑248 (MQ=255)
                                                                                                                                                                                                                       tCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAAATTCGTCGTTTAATGCACGGTAACCGAAGAAGTCACCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATCCGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCAACGCCgtcaattgacccagcaata                            >  2:405094/1‑232 (MQ=255)
                                                                                                                                                                                                                                tgtgGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATAGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCAAACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTT‑ACCATTCGCGCGGCAGGATCATCGCCGGAACTTTCCCCAGCAGTATCGGTAAcc                   >  2:208700/1‑249 (MQ=255)
                                                                                                                                                                                                                                   ggACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCg               <  1:377670/251‑1 (MQ=255)
                                                                                                                                                                                                                                                tCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCACAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTACCCCAGCAATAACGGTAACCAGCGGGCCGGTTGCGTg  >  2:677573/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
CGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTG  >  NC_000913/76114‑76604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑