| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 75,920 | G→C | T460T (ACC→ACG) | sgrR ← | DNA‑binding transcriptional dual regulator SgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 75,920 | 0 | G | C | 93.8% | 201.7 / 5.0 | 64 | T460T (ACC→ACG) | sgrR | DNA‑binding transcriptional dual regulator SgrR |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base C (30/30); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
AAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTA‑GCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTTAGGGTGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGG > NC_000913/75677‑76166 | aaaaTCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGCGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGt > 1:246722/1‑251 (MQ=255) tCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTgac > 1:526431/1‑249 (MQ=255) cGAACCAGCCGTGGGGATTCATGCGTAGGCCGCGCCTACTGCGTTGCCCCTTAATGAGCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGgcg < 2:653597/251‑1 (MQ=255) cccAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTg < 2:595788/250‑1 (MQ=255) aCCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCCCCTCGCACAAGTGCGCGAACAGCGGA‑AAATCCAGCGGCAGCGTAAAGTTGGCGCTg > 2:167223/1‑251 (MQ=255) ggTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCGCCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑Gcc > 1:205906/1‑248 (MQ=255) cgtaGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGACGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGatagcgctctc > 2:536089/4‑243 (MQ=255) cAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTA‑GCCAGATATCACTTTCGa < 2:431276/251‑1 (MQ=255) aGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCa > 1:143648/1‑236 (MQ=255) aGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCa < 2:143648/236‑1 (MQ=255) aTACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCCGATATCGCTCTCGATCcctccttc > 2:551284/1‑249 (MQ=255) gCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGa < 1:162673/251‑1 (MQ=255) gCCCCGGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTg < 1:709384/250‑1 (MQ=255) tGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCTCTGttaa < 2:48133/208‑3 (MQ=255) tGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGttaa > 1:48133/1‑206 (MQ=255) gAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTTATTTCGCCAGTGCGCCAGCGCGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTa < 1:113786/250‑1 (MQ=255) gATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCCCTCCTTCATGCCACTGAGCGTAgcgg < 2:152535/250‑4 (MQ=255) aTCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGGGTTGGAGCAGCGGCCCCTCGCACACGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGGTGGCGCTGTTAA‑GCCAGAAATCGCTCTAGATCTCTCCTTCCTGCCACTGATCGTAgctgat > 2:519518/1‑246 (MQ=255) gtggataagcggCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAttt < 1:17773/241‑1 (MQ=255) tggataagcggCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCTTTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTCA‑GCCAGATATCGCTCTCGATCTCTCCTTAATGCCACTGATCGTAGCTGTTCTCTTTGAtttc > 1:125343/10‑250 (MQ=255) ggCACCATTGCTTGGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGCTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGAGCGTAGCTGATCTCTTTGATTTCCAGCGTGAc < 2:121576/251‑1 (MQ=255) gCACCAACGCTTTGCTGGCGACCAGTTGCTGGCCCCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGGGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTGA‑GCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGAcc < 2:249012/251‑1 (MQ=255) cACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCt < 1:217257/251‑1 (MQ=255) aCCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGCTCGGAATCCAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGAtctc > 2:406102/1‑200 (MQ=255) aCCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCGCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGAtctc < 1:406102/200‑1 (MQ=255) attgCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATAGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTg > 2:513627/4‑251 (MQ=255) cTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGAGGCACCTCGCACAAGTGCGCGAA‑CAGCGCAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATAACGCTCTCGATCCCTCCTTCATGCCACTGATCGTAGCTAATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGcccg > 2:541533/1‑249 (MQ=255) cAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATctgct < 1:519518/251‑1 (MQ=255) tGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGCTTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCAt > 2:560336/1‑251 (MQ=255) ggcgggCACCAGTTCGCCAGGTTCATTTCGCCAGTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCCCCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCAt < 2:635438/247‑1 (MQ=255) tcgccagggtcattgcGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCTCCTCGCACAAGCGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAAt < 2:246722/235‑1 (MQ=255) cgggttcattccGCCATTGCGCCAGCGGGCGGCGGCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAAGCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCAccc < 2:277345/240‑1 (MQ=255) aggttCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCg > 1:350258/4‑251 (MQ=255) atttcGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTc > 1:703806/4‑251 (MQ=255) cGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTccatg > 2:143041/1‑247 (MQ=255) cGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACtg > 1:58436/1‑251 (MQ=255) aTTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGAt < 1:317737/251‑1 (MQ=255) tGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATc < 1:455182/250‑1 (MQ=255) ggcgcCAGCGGGCGGGGGCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAATTTGGCGCTGGTAA‑GCCAGATATCGCTCTCTATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCTCCCGATGCTCACTGTGATcc < 2:13816/250‑1 (MQ=255) cgcCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCTGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTg < 1:210514/251‑1 (MQ=255) gggcggcGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATaaa > 1:36657/6‑249 (MQ=255) gggcggcGTCGGCTTGCCAGTCGATCGGAATACAGGGTGGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCACTCTCGATCTCTCCTTCAGGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGCTaaagg > 1:181635/6‑249 (MQ=255) ggcggcGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGGGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAgg < 2:667825/247‑1 (MQ=255) ggcGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATaaa > 2:170534/2‑245 (MQ=255) ggcGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATaaa < 1:170534/244‑1 (MQ=255) ggcGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGtc > 1:301539/2‑249 (MQ=255) ggcGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGGTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACGGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATTCTCACTGTGATCCTGATAAAAGGTCAgga < 2:620400/249‑2 (MQ=255) gTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAgga < 1:551284/251‑2 (MQ=255) ccAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCttt > 1:702439/1‑251 (MQ=255) ccAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTA‑GCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTTAGGGTGAGGCttt > 1:268914/1‑251 (MQ=255) cagtcgatcggcatacagtgttggAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTACAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTACATCGTGACCTGGTGACTTGCCAGCATCTGCTGCATGATCCCGGCAATCACCCGCTGCTCACTGTGATCCTGATAAAAGGTCCGGATGAGGCTTTc > 2:518493/19‑251 (MQ=255) agtcgatcggAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTcc < 1:12260/243‑1 (MQ=255) cgatcggAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAgg > 1:494151/6‑251 (MQ=255) gatcggAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGCGGCTTTcccggg > 2:541974/5‑247 (MQ=255) cggAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCGTTCCAGGCCAg < 2:242798/250‑1 (MQ=255) gtgttggAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGCTAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGttttt > 1:397623/2‑251 (MQ=255) tCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGATGTTTA‑GCCAGATATCATTTTCGATCGCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTTAGGGTGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCgg < 1:249174/249‑1 (MQ=255) tgcgcgaacagcgaaaaatCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGcc > 2:249124/19‑250 (MQ=255) gcgaacagcgaaaaatCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGATGCCAACg > 1:610071/16‑251 (MQ=255) gaacagcgaaaaatCCAGCGGCAGCGTAAAGTTGGCGCTGTTAC‑GCCAGATATCGCTCTCGATATCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGCTGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCACTTTATGGGGCGGGCCTGGTGCAAACggg > 2:41020/14‑251 (MQ=255) agcgaaaaatCCAGCGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAg < 1:9836/242‑1 (MQ=255) cGGCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCg < 1:541119/251‑1 (MQ=255) gCAGCGTAAAGTTGGCGCTGTTAA‑GCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCggg < 1:41020/251‑1 (MQ=255) gCAGCGTAAAGTTGGCGC‑GTTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTTACCTGGTGACTTGCCAGAATCCGCTGCATGAGCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCggg < 2:449298/251‑1 (MQ=255) | AAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTA‑GCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTTAGGGTGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGG > NC_000913/75677‑76166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |