Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,057	G→T	L242I (CTT→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,057	0	G	T	100.0%	232.4 / NA	71	L242I (CTT→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/26);  total (45/26)

TCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCG  >  NC_000913/64813‑65300                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    tCGGAGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACCCC‑TGAGCGTCGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGGCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACg                                                                                                                                                                                                                                                  <  2:80321/248‑1 (MQ=255)         aCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCCAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCTCCCCAGCCGCAACGGAATACGGTAACGCTCgg                                                                                                                                                                                                                                       >  1:429507/1‑251 (MQ=255)              ggTCAATTTCGTCCATTCGCTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCCACGGAATACGGTAACGCTCGGCATGt                                                                                                                                                                                                                                  >  2:648453/1‑251 (MQ=255)                 cAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGtttt                                                                                                                                                                                                                               <  1:190035/251‑1 (MQ=255)                 cAATTTCGCCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGtttt                                                                                                                                                                                                                               <  2:114403/251‑1 (MQ=255)                      cacccgccgctcttccgatctttATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAAGGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                                                                                                                                                                                                            <  2:207087/228‑1 (MQ=255)                            agtctaccccACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCTACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCTGCATGTTTTTGCAGCATTCg                                                                                                                                                                                                                    <  2:171947/244‑1 (MQ=255)                              ccgaacccACGGGTTATCGATAGAGTTTCCTTAGCTAAATAACCCC‑TGAGCGACGGTTTCCAGCGAGAAGGAAGAGAAAGTCCAGAACGCGGATATCAGCGCCTCGATACCGCCGATAATTAGCCGTCTGTTAGCCTGGGCAAAAAAGACGCCGCTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCa                                                                                                                                                                                                                  <  2:363444/246‑1 (MQ=255)                               cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                                   >  1:515426/1‑249 (MQ=255)                                gATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                                   >  2:91577/1‑248 (MQ=255)                                       cGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCA‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGCTAATTAGCCGACCTTTAGCCTGGGCACAAAAGACGCCGTTTTTAAAGCCGGTCTCGCGACACTCAAGCTAACTGTTATCGCGCCCCAGCCCCAGAGGAATCCGGGAACGCTCGACATGTTTTTGCAGAATTCGACGATCGAACt                                                                                                                                                                                                         >  2:167115/1‑251 (MQ=255)                                           ttATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                                                                                                                                                                                                            >  1:207087/1‑228 (MQ=255)                                                gaacgATTTTCCTTCGCCTAATA‑CTCCCTGCGCGTCGGTTTCCAGCGGGAATGAAGAGAAATTCCAGAACCCGGATTTCAGCGCCCCGATACCGTTGATAATTAGCTGTCCTTTAGCCTGGGAAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACGCCAGCTCACTGTTATTGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCCCCACGt                                                                                                                                                                                                <  2:563692/247‑1 (MQ=255)                                                   aGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTCCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTcc                                                                                                                                                                                             <  2:515426/251‑1 (MQ=255)                                                       tttCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAAcc                                                                                                                                                                                         >  1:309764/1‑251 (MQ=255)                                                         tCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCg                                                                                                                                                                                        >  1:45765/1‑250 (MQ=255)                                                                   aaTAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcaca                                                                                                                                                                               <  1:126111/249‑1 (MQ=255)                                                                   aaTAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcaca                                                                                                                                                                               <  1:630101/249‑1 (MQ=255)                                                                    ataacTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGTAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTTACGCTCGGCATGTTTTTGCAGCATTCGCCGCTCGAACTGCACCACGTTCCAACCGATGATCCCATc                                                                                                                                                                             >  2:258972/5‑250 (MQ=255)                                                                               aGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGt                                                                                                                                                                  >  1:376303/1‑251 (MQ=255)                                                                                gCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATCCGGTAACGCTCGGCATGTTTTTGCCGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGTTCGTAGtt                                                                                                                                                                 >  2:167830/1‑251 (MQ=255)                                                                                gCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGtt                                                                                                                                                                 <  2:146901/251‑1 (MQ=255)                                                                                 ggacggTTTCCAGCGAGAATAAAGAGAAATTCCAGAACGCGGATTTCATCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTg                                                                                                                                                                <  2:434528/246‑1 (MQ=255)                                                                                 cGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTg                                                                                                                                                                >  1:96670/1‑251 (MQ=255)                                                                                  gacggTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTgg                                                                                                                                                               >  1:576702/5‑251 (MQ=255)                                                                                    cacgacgctcgctcgatacagagAAAGTCCAGACCCCGGATTTCAGCGCTTCGAGCCCTTATATAATTAGCCGACCTTTTGCCTGGGCAAAAAAGACGCCGTTTTGAAAGCCGGGCACGCGCCACTCCAGCTCTCTGTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGAGCACATCAGGATCGTAGTTGGCa                                                                                                                                                             <  2:368130/232‑1 (MQ=255)                                                                                     ggTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgcag                                                                                                                                                                                                                          <  2:17692/188‑1 (MQ=255)                                                                                     ggTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgcag                                                                                                                                                                                                                          >  1:17692/2‑189 (MQ=255)                                                                                          ccAGCGAGAATGAAGAGAAATTCCAGACCGCGGATTTCAGCGCCTCTGTACCGTCTATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGAGCACAGCAGGATCGTAGTTGGCAAACCa                                                                                                                                                        <  2:364266/250‑1 (MQ=255)                                                                                          ccAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAg                                                                                                                                                       <  1:466846/251‑1 (MQ=255)                                                                                                   aTGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGt                                                                                                                                              >  1:545036/1‑251 (MQ=255)                                                                                                      aaGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAATCCAGGCGTTGAGtttt                                                                                                                                           <  1:574104/251‑1 (MQ=255)                                                                                                       agagAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATCCGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCa                                                                                                                                                             >  1:368130/1‑232 (MQ=255)                                                                                                              ttCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgt                                                                                                                                     >  1:13384/1‑248 (MQ=255)                                                                                                                 cAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgtagctg                                                                                                                                >  1:379666/1‑245 (MQ=255)                                                                                                                    aaCGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCg                                                                                                                                                                                                                                        >  1:425848/1‑144 (MQ=255)                                                                                                                     aCGCGGATTTCAGCGCCTCGATACCGGCGATTATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCACAGCCGCAGCGGTATACGGTCACGCTCGGCATGTTTTTGCAGCCTTCGCAGATCGAACTGCACCACGTTCCAACCGCTGATCACATCAGGATCGTAGTTGGAACACCAGGCGTTGAGTTTTTCCGGTAGCTgcggg                                                                                                                            >  2:60542/1‑251 (MQ=255)                                                                                                                       ccgGATTTCAGCGCCTCGTTACCGTCATTAATTAGCCGCCCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCGCGCGCCACTCCAGCTCCCTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggcg                                                                                                                          <  2:403346/250‑1 (MQ=255)                                                                                                                         ggATTTCAGCGCCTCGATACCGTCGCTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGGGTTATTCCAGTAGCTGCGggcggc                                                                                                                        >  2:278024/1‑251 (MQ=255)                                                                                                                                            ccGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTc                                                                                                     >  1:32553/1‑251 (MQ=255)                                                                                                                                                   tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCCCTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGAATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGGGACGTATTCCGGTTCAAAATcg                                                                                              >  2:2371/1‑250 (MQ=255)                                                                                                                                                    aaTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCCGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTcaacatcgg                                                                                             >  2:316419/1‑243 (MQ=255)                                                                                                                                                     aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATcg                                                                                              >  1:710125/1‑248 (MQ=255)                                                                                                                                                         cccGACCTTTAGCCGGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCCCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCTTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcg                                                                                        <  1:665911/250‑3 (MQ=255)                                                                                                                                                               cTTTAGCCTGGGCAAAACAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGAGCGGAGGCg                                                                                  >  1:154294/1‑251 (MQ=255)                                                                                                                                                                tttAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGt                                                                                 >  1:123459/1‑251 (MQ=255)                                                                                                                                                                tttAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGt                                                                                 >  1:634338/1‑251 (MQ=255)                                                                                                                                                                             aaaaaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctctgg                                                                    <  1:170983/251‑4 (MQ=255)                                                                                                                                                                                aaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGCTCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGCAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGAGGCTAGCGACGTAATCCAGTTCAAAATCGATCGCGAAGGCGTCGCCATTATCTGGccc                                                                 >  2:485439/1‑251 (MQ=255)                                                                                                                                                                                aaGACGCCGTTTTTAAAGCCGTGCTCGAGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCGCACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGGTGGCGTCGTATTCCAGATCAAAATCGCGCGGTGAGGCGACGCCATtcctgggccc                                                                 >  2:80995/1‑243 (MQ=255)                                                                                                                                                                                 aGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGGATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcccc                                                                >  1:465526/1‑251 (MQ=255)                                                                                                                                                                                   ccGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAg                                                              <  1:507344/250‑1 (MQ=255)                                                                                                                                                                                              aaagcctgccccgcgCCACCCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCCCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGa                                                   <  2:576702/240‑1 (MQ=255)                                                                                                                                                                                               aaGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGAt                                                  >  1:255463/1‑251 (MQ=255)                                                                                                                                                                                                 gCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACAAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGATGGCGACGTATTCCAGTTCAAAATCGCGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATgc                                                >  1:150555/1‑251 (MQ=255)                                                                                                                                                                                                      gCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGGTCAAAATCGAGCGCGGCGGCGTCGCAATACTCTGGCCCCCGCATAAAAACGATGCGCTGc                                           >  2:531674/1‑251 (MQ=255)                                                                                                                                                                                                            gcCACTCCAGCTCACTGTTATCGCGCCCCACCCGCAGCGGACTACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTTGCGCAGTATTCCATTTCAAAATCGGGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAACCGGTGCGCTccccccac                                     >  2:438738/1‑247 (MQ=255)                                                                                                                                                                                                             ccACTCCAGCTCACTGGTAACGCGCCCCAGCCGCAGCGGAATACGGTAACGCGCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGc                                    <  2:45765/251‑1 (MQ=255)                                                                                                                                                                                                               aCTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCt                                  >  1:163984/1‑251 (MQ=255)                                                                                                                                                                                                               aCTCCAGCTCACTGTTATCGCGCCCCACCCGCAGCGGCATACGGTAACGATCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGCTGATCCCATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCATAATCGAGCCACGAGGCGTCGCCATTATCATGCCCCAGCATATaacacatgccctcaccgcatact                                  >  2:303213/1‑230 (MQ=255)                                                                                                                                                                                                                  ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTc                                <  1:91577/250‑1 (MQ=255)                                                                                                                                                                                                                   cAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                              <  1:352892/251‑1 (MQ=255)                                                                                                                                                                                                                               atCGCGCCCCAGCCGCAGCGGCATAAGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGCTCCAACCAATGATCACCTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCAAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGCGCGAGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGt                  >  1:350361/1‑251 (MQ=255)                                                                                                                                                                                                                               atCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGt                  >  1:705545/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgcgCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                <  2:13384/251‑1 (MQ=255)                                                                                                                                                                                                                                    gccccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGc             >  1:36639/9‑251 (MQ=255)                                                                                                                                                                                                                                        cagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCAc         >  1:157707/5‑251 (MQ=255)                                                                                                                                                                                                                                        cagccGCAGCAGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGCTCACATAGGGATCGTCGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAACATCGAGCGCGGAGGCGACGCCATTATCTAGCCCCAGCATATAACCGCTGAGCAGCCCGCAGCCTTCCAGGCCGCTGCAACACAGCTCAc         >  2:640415/5‑251 (MQ=255)                                                                                                                                                                                                                                             gCAGCGGAATTCGGTAACGCTAGGCATGTTTTTGCAGCATTCGCAGATCGAACTGAACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAGACCCGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGAGGTGGGGTCGACATTCTCTGGCCCCAGCCTATAAAAGATGCGCTGCCCGCAGCCTTCCAGGCCGAGGCAGTACAGCTCACCGTgg    >  2:81236/1‑251 (MQ=255)                                                                                                                                                                                                                                               aGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCg  >  1:99458/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ggAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcggcgg                                                                                    >  1:406232/1‑160 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCG  >  NC_000913/64813‑65300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑