Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 75,707 C→T L531L (CTG→CTA)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091375,7070CT96.5% 182.0 / ‑4.2 58L531L (CTG→CTA) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (29/26);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑CAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑CAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGA  >  NC_000913/75460‑75957
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                             
gggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAgg                                                                                                                                                                                                                                                            >  1:620400/1‑251 (MQ=255)
 ggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGc                                                                                                                                                                                                                                                           <  1:388112/251‑1 (MQ=255)
      aCATTGTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCgcgc                                                                                                                                                                                                                                                      <  1:686793/251‑1 (MQ=255)
          ttttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATAc                                                                                                                                                                                                                                                  >  1:361804/1‑251 (MQ=255)
               tAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGCACCAGCCGAGGGTATTCATGCGTCAG‑CCGCGCATACTGCgg                                                                                                                                                                                                                                             >  1:708904/1‑250 (MQ=255)
                 acacTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTg                                                                                                                                                                                                                                           >  1:242798/1‑251 (MQ=255)
                           ccacAAAAAAGAGTGGCAAAGGACTTGATAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATg                                                                                                                                                                                                                                 <  1:312489/248‑1 (MQ=255)
                                aaaaaGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATc                                                                                                                                                                                                                              >  1:152535/1‑249 (MQ=255)
                                  aaaGAGTGGCAAAGGACTTGAGCAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTCCCCCAGCAATCATGGATCCGGTGGCGCAACCCACGCTGTTTTCAAATCGAACCAGCCGAGGGCATTCATGCG‑TAGGCCGCGCATAc                                                                                                                                                                                                                                                  >  2:261232/1‑227 (MQ=255)
                                  aaaGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATAc                                                                                                                                                                                                                                                  <  1:261232/227‑1 (MQ=255)
                                  aaaGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGcc                                                                                                                                                                                                                          >  1:576214/1‑251 (MQ=255)
                                  aaaGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGcc                                                                                                                                                                                                                          <  1:559974/251‑1 (MQ=255)
                                          gCAAATGACATGAGAAAGAGCCTCAAATCCATTCGCCGGCGTTAACCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGTCGTTAGCGTAGTGATTTTGATATCAACCAGCAATCCTGGTTCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGACGAGGGTATTCCTGCG‑GAGGCCGCGCATACTACGTTGCCCCTAATTGATCAGCAAgtggtgg                                                                                                                                                                                                                  >  2:510839/1‑250 (MQ=255)
                                             aaGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCCGCCAgtggtgga                                                                                                                                                                                                                 >  2:154523/1‑247 (MQ=255)
                                             aaGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtgga                                                                                                                                                                                                                 <  1:154523/249‑3 (MQ=255)
                                               ggACTTGCGAAGGAGCCTCAAGTCCCTTCGCCGGCGATATCCGGATCAGGTTCGACAGGGATTTTCTCAGCGCACGCGTACGAGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATAGATCCGGTGGCGCAAACCACACTGATTTAAAAACGAACCAGCCGAGGGTAATCATGCG‑TAGGCCGCGCATACTAAGTTGCACCTGACTTATCAGcacggggtgacta                                                                                                                                                                                                               >  2:605551/1‑237 (MQ=255)
                                                                              cGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGc                                                                                                                                                                            <  1:525507/251‑1 (MQ=255)
                                                                                 cGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTgg                                                                                                                                                                         >  1:602795/1‑251 (MQ=255)
                                                                                 cGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTgg                                                                                                                                                                         >  1:635438/1‑251 (MQ=255)
                                                                                   ttATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGCACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGCTTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACAATTGCTTTGCTGTCTGCCAGTTGCTGGc                                                                                                                                                                        >  2:312596/1‑250 (MQ=255)
                                                                                        cggctCAGGTTCGACGGGTATTTTCTCAGCGCACGAGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCACCCAGCAATCATGGCTCCGGTGGCGCAAACCACGCTGATTTAAAATCGACCCAGCCGAGGGTGTTCATGCT‑TAGGCCGCGCATACTGGGGTGCCCCTGAATGATCAGCCAGGGGTG‑‑GTTAAGCGGCACCATTGCTTTGCGGGCGACGCGTTGCTgaccccagg                                                                                                                                                                  >  2:455182/5‑243 (MQ=255)
                                                                                        cGGATCCGGGTCGGCGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTTATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGt                                                                                                                                                                  <  2:33579/251‑1 (MQ=255)
                                                                                        cGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGAGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCCCGCTGATTTAAAATCGCACCAGCCGAGGGAATTCATGCG‑TAGGCCACGCATACTGCGTTGCCCCTGAATGTTCAGCCAGTGGGG‑GAGAAG‑CGGCACCATTGCTTTGCTGGGGACCAGTAGCGGGCCCCAGt                                                                                                                                                                  >  2:633511/1‑251 (MQ=255)
                                                                                          gATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc                                                                                                                                                                <  1:223587/251‑1 (MQ=255)
                                                                                          gATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc                                                                                                                                                                >  1:667825/1‑251 (MQ=255)
                                                                                             cAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGAG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGcc                                                                                                                                                             <  1:639120/251‑1 (MQ=255)
                                                                                                       gggTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGATTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCCGGTTCAttt                                                                                                                                                   >  2:541119/1‑249 (MQ=255)
                                                                                                        ggTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑CAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑CAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCAtctc                                                                                                                                                  <  1:357616/251‑1 (MQ=255)
                                                                                                         gTATTTTCTCAGCGCACGCGTACGCGTGTCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCg                                                                                                                                                 <  1:157712/251‑1 (MQ=255)
                                                                                                                 tcAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTgcg                                                                                                                                         >  2:548215/1‑251 (MQ=255)
                                                                                                                     cgcACGAGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGAACAGTTGCAGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGcc                                                                                                                                       >  1:607982/1‑249 (MQ=255)
                                                                                                                         cgcgtacgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCggg                                                                                                                                 <  1:421109/251‑3 (MQ=255)
                                                                                                                          gcgtacgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCgggc                                                                                                                                >  1:277345/1‑248 (MQ=255)
                                                                                                                           cgtacgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCgggcg                                                                                                                               >  1:653597/1‑247 (MQ=255)
                                                                                                                            gtacgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGGATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGGCTA‑AG‑CGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCCCCAGTTCGCCCGGTTCATTTCGCCATTGCGCCAGCgggcgg                                                                                                                              >  2:50156/1‑246 (MQ=255)
                                                                                                                              acgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCa                                                                                                                                                            <  1:271217/219‑1 (MQ=255)
                                                                                                                              acgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTAGCGACCAGTTGCTGGCACCAGTTCGCCa                                                                                                                                                            >  2:271217/1‑219 (MQ=255)
                                                                                                                                gcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTc                                                                                                                          <  1:708454/251‑1 (MQ=255)
                                                                                                                                 cgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCg                                                                                                                         <  1:195076/251‑1 (MQ=255)
                                                                                                                                    ggCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCGTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCt                                                                                                                      <  1:678765/251‑1 (MQ=255)
                                                                                                                                            gTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAAGCAGGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGGATTCATGCG‑TAGGCCGCGCATACTGCTTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCTGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCGAGGTGCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTc                                                                                                              <  1:643490/251‑1 (MQ=255)
                                                                                                                                                agaACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTcgatc                                                                                                          <  1:98900/251‑5 (MQ=255)
                                                                                                                                                  aaCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTcgatcgg                                                                                                        >  1:121339/1‑245 (MQ=255)
                                                                                                                                                              gtagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGCTTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTTCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATTCAGtgttg                                                                                            >  2:12260/1‑251 (MQ=255)
                                                                                                                                                                      ttttttATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAgc                                                                                     <  2:576214/246‑2 (MQ=255)
                                                                                                                                                                                aCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCa                                                                          <  1:50156/251‑1 (MQ=255)
                                                                                                                                                                                aCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCa                                                                          <  1:548215/251‑1 (MQ=255)
                                                                                                                                                                                              agccGGTGGCGCAATCCCCGTGGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaaca                                                            <  2:158651/249‑3 (MQ=255)
                                                                                                                                                                                              aTCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaac                                                             >  1:206706/1‑249 (MQ=255)
                                                                                                                                                                                                       cgcAAACCACGCTGATTGAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaat                                                   <  1:489903/251‑12 (MQ=255)
                                                                                                                                                                                                             ccACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatccagcg                                             >  1:449298/1‑234 (MQ=255)
                                                                                                                                                                                                                         aaaaTCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGCGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGt                                >  1:246722/1‑251 (MQ=255)
                                                                                                                                                                                                                             tCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTgac                            >  1:526431/1‑249 (MQ=255)
                                                                                                                                                                                                                              cGAACCAGCCGTGGGGATTCATGCG‑TAGGCCGCGCCTACTGCGTTGCCCCTTAATGAGCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGgcg                           <  2:653597/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cccAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTg                        <  2:595788/250‑1 (MQ=255)
                                                                                                                                                                                                                                 aCCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCCCCTCGCACAAGTGCGCGAACAGCGGA‑AAATCCAGCGGCAGCGTAAAGTTGGCGCTg                        >  2:167223/1‑251 (MQ=255)
                                                                                                                                                                                                                                     gCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAGACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatcc                                                 <  1:76857/223‑14 (MQ=255)
                                                                                                                                                                                                                                           ggTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCGCCTCGCACAAGTGCGCGAA‑CAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGcc                 >  1:205906/1‑248 (MQ=255)
                                                                                                                                                                                                                                                        cAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑CAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGa  <  2:431276/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                             
GGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑CAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑CAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAA‑AACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGA  >  NC_000913/75460‑75957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑