Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,896	A→G	T295T (ACT→ACC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,896	0	A	G	98.0%	155.6 / ‑4.2	50	T295T (ACT→ACC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (26/23);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

TAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTG  >  NC_000913/64651‑65142                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTTTAGCTAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTc                                                                                                                                                                                                                                                   >  1:113001/1‑251 (MQ=255)    agaggaccaatctccgccACCGATCCTCCGTGGCGGTTTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAAGCTGCGTCACCAGCTCGCAATCTCTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGGTCAAGTTCGTCCATTCGATCCCACGGGTTATCGATAGAGTTTCCTACGCCTAATAACTCC‑TGAGCGACGGTTTCCAg                                                                                                                                                                                                                                                <  2:76227/238‑1 (MQ=255)         aCCAAACGCCGCCACCGATCCCCCGTGGCGTTCTACCGGCAGGCCGTTTACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCGGCGAAACGGCGGTCAATTTCGTCCATGCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCgaga                                                                                                                                                                                                                                           <  1:444914/251‑1 (MQ=255)            aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGGCGGGTTCCAGCGGGAATg                                                                                                                                                                                                                                        >  1:403346/5‑251 (MQ=255)            aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATg                                                                                                                                                                                                                                        <  2:306315/247‑1 (MQ=255)                                     cGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCATCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGAttt                                                                                                                                                                                                               <  2:260320/251‑1 (MQ=255)                                          cACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGTTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                                                                                                                                                                          <  1:157314/251‑1 (MQ=255)                                           aCCGGCAGGCCGTTCACCGTCGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTAAGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                                                                                                                                                                          <  2:330005/250‑1 (MQ=255)                                              ggCAGGCCGTTCTCCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGTTTTATCTTCGGCGAAACGGCTGTCAATTTCGTCCAGTCGTTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                                                                                                                                                                      <  1:688701/251‑1 (MQ=255)                                              ggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGCTTTATGGAAAATCTGCGTCACCAGCTCGCAATCATTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTGCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACGCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                                                                                                                                                                      <  2:118258/251‑1 (MQ=255)                                                          aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGAt                                                                                                                                                                                          <  1:13574/251‑1 (MQ=255)                                                                 cgcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTGTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCTATAATTa                                                                                                                                                                                     <  2:650974/247‑1 (MQ=255)                                                                       cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTGCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCt                                                                                                                                                                             <  1:65577/251‑1 (MQ=255)                                                                                        aTTTCAGTTTTCTGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGAGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGCTCCCACGGGTTATCTATAGATTTTCCTTCGCCTAATCACTCC‑TGCGCGACGGGTTCCAGCGAGAATGAAGAGAAAATCCCGAAAGCGTATTTCAGCGCCTCGATACCGTCGATAATTATACGCCATTTGGCCTTGGCaaaaa                                                                                                                                                             >  2:78215/1‑250 (MQ=255)                                                                                         tttCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCCATTTTGGCCCTTCGATTCCACGGGTTATCTATAGATTTTTCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGGGTATGAATAGAAATTCCAGAACCCGGATTTCAACACCTCGATACCTCCGCTAATTCGTCGGCCTTTAGACTGGGCaaacaa                                                                                                                                                            >  1:269291/1‑247 (MQ=255)                                                                                                tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGt                                                                                                                                                    >  1:702365/5‑251 (MQ=255)                                                                                                tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGTAAAAAAGACGCCg                                                                                                                                                     <  1:555262/246‑1 (MQ=255)                                                                                                     ggAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGGTAATTTGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGgtttt                                                                                                                                                >  2:466846/1‑250 (MQ=255)                                                                                                       aaaaTCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTaa                                                                                                                                              >  1:434528/4‑250 (MQ=255)                                                                                                            cggCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCACGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                                                                                                                                        <  2:124074/249‑1 (MQ=255)                                                                                                               cGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCCTTCGATCCCCCGGGTTATCGATAGATTTTCCTTCTCCTAATAACTCC‑TGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCCGCGCCTCGATACCTTCGATAATTAGCCGACCTTTAGCCTGGGCAAACAAGTCGCCGTTTTTAAAGCCGTGCt                                                                                                                                     >  2:126111/1‑251 (MQ=255)                                                                                                                 tcaccagccagaaaTCTTTCAGGTTATCAGTAGCCAGAGCCGGTTTATATTCGGCGAAACGGCGGACAACTTAGTCCATTCGATCCCACGGGTTAACCATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGAAGGTTTCCCGCGCGGATGAAGAGAAATTCCAGCACCCGGATTTCCGCGCCCCGATCCCGACGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCAGTTTTTAAAGCCgcgcctg                                                                                                                                   >  2:352892/13‑245 (MQ=255)                                                                                                                                   tCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGGTTTCAGCGCCTCGATACCGTCGGTAATTATCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGGGATCGCGCCACTCCACCTcactg                                                                                                                 >  2:263346/1‑247 (MQ=255)                                                                                                                                      ggTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgtt                                                                                                               >  1:146901/1‑244 (MQ=255)                                                                                                                                                     gcgcATGTTTATCTTCGGCGTAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTTTCGATAGATTTTCCTTTGCCTCATTACTCC‑TGAGCGCCGGTTTCCCCCGAGAATTACAAGAAATTCCAGCACGCGGGTTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTATCCTGGGCCAAAAAGACGCCGTTTTTTACGCCGTTCTCTCGCCCCTCCCCCTCCCTGTTTTCGCGccccagcccc                                                                                               >  2:52521/1‑244 (MQ=255)                                                                                                                                                            tttATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGa                                                                                         >  1:228785/1‑250 (MQ=255)                                                                                                                                                                 cTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGactacgg                                                                                   >  1:498561/1‑245 (MQ=255)                                                                                                                                                                   tCGGAGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACCCC‑TGAGCGTCGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGGCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACg                                                                                    <  2:80321/248‑1 (MQ=255)                                                                                                                                                                           aCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCCAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCTCCCCAGCCGCAACGGAATACGGTAACGCTCgg                                                                         >  1:429507/1‑251 (MQ=255)                                                                                                                                                                                ggTCAATTTCGTCCATTCGCTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCCACGGAATACGGTAACGCTCGGCATGt                                                                    >  2:648453/1‑251 (MQ=255)                                                                                                                                                                                   cAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGtttt                                                                 <  1:190035/251‑1 (MQ=255)                                                                                                                                                                                   cAATTTCGCCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGtttt                                                                 <  2:114403/251‑1 (MQ=255)                                                                                                                                                                                        cacccgccgctcttccgatctttATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAAGGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                                              <  2:207087/228‑1 (MQ=255)                                                                                                                                                                                              agtctaccccACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCTACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCTGCATGTTTTTGCAGCATTCg                                                      <  2:171947/244‑1 (MQ=255)                                                                                                                                                                                                ccgaacccACGGGTTATCGATAGAGTTTCCTTAGCTAAATAACCCC‑TGAGCGACGGTTTCCAGCGAGAAGGAAGAGAAAGTCCAGAACGCGGATATCAGCGCCTCGATACCGCCGATAATTAGCCGTCTGTTAGCCTGGGCAAAAAAGACGCCGCTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCa                                                    <  2:363444/246‑1 (MQ=255)                                                                                                                                                                                                 cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                     >  1:515426/1‑249 (MQ=255)                                                                                                                                                                                                  gATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                     >  2:91577/1‑248 (MQ=255)                                                                                                                                                                                                         cGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCA‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGCTAATTAGCCGACCTTTAGCCTGGGCACAAAAGACGCCGTTTTTAAAGCCGGTCTCGCGACACTCAAGCTAACTGTTATCGCGCCCCAGCCCCAGAGGAATCCGGGAACGCTCGACATGTTTTTGCAGAATTCGACGATCGAACt                                           >  2:167115/1‑251 (MQ=255)                                                                                                                                                                                                             ttATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                                              >  1:207087/1‑228 (MQ=255)                                                                                                                                                                                                                  gaacgATTTTCCTTCGCCTAATA‑CTCCCTGCGCGTCGGTTTCCAGCGGGAATGAAGAGAAATTCCAGAACCCGGATTTCAGCGCCCCGATACCGTTGATAATTAGCTGTCCTTTAGCCTGGGAAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACGCCAGCTCACTGTTATTGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCCCCACGt                                  <  2:563692/247‑1 (MQ=255)                                                                                                                                                                                                                     aGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTCCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTcc                               <  2:515426/251‑1 (MQ=255)                                                                                                                                                                                                                         tttCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAAcc                           >  1:309764/1‑251 (MQ=255)                                                                                                                                                                                                                           tCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCg                          >  1:45765/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaTAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcaca                 <  1:630101/249‑1 (MQ=255)                                                                                                                                                                                                                                     aaTAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcaca                 <  1:126111/249‑1 (MQ=255)                                                                                                                                                                                                                                      ataacTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGTAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTTACGCTCGGCATGTTTTTGCAGCATTCGCCGCTCGAACTGCACCACGTTCCAACCGATGATCCCATc               >  2:258972/5‑250 (MQ=255)                                                                                                                                                                                                                                                 aGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGt    >  1:376303/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATCCGGTAACGCTCGGCATGTTTTTGCCGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGTTCGTAGtt   >  2:167830/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGtt   <  2:146901/251‑1 (MQ=255)                                                                                                                                                                                                                                                   cGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTg  >  1:96670/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTG  >  NC_000913/64651‑65142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑