Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,883	G→A	L300L (CTA→TTA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,883	0	G	A	95.9%	137.5 / ‑2.0	50	L300L (CTA→TTA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (23/24);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

ATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACA  >  NC_000913/64639‑65127                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑Tg                                                                                                                                                                                                                                                <  1:701523/245‑1 (MQ=255)   acgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGGTAGATTTTCCTTCCCCTAATAACTCC‑Tggg                                                                                                                                                                                                                                              >  1:80321/5‑249 (MQ=255)      ggaaaaaTAGAAATGCCCAAGTGCCGCCCCGGAACCGCCGGGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGa                                                                                                                                                                                                                                           <  2:211800/248‑1 (MQ=255)       ggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGAc                                                                                                                                                                                                                                          <  1:162730/251‑1 (MQ=255)        gAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGAcg                                                                                                                                                                                                                                         <  1:656035/251‑2 (MQ=255)             tAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTTTAGCTAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTc                                                                                                                                                                                                                                    >  1:113001/1‑251 (MQ=255)                agaggaccaatctccgccACCGATCCTCCGTGGCGGTTTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAAGCTGCGTCACCAGCTCGCAATCTCTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGGTCAAGTTCGTCCATTCGATCCCACGGGTTATCGATAGAGTTTCCTACGCCTAATAACTCC‑TGAGCGACGGTTTCCAg                                                                                                                                                                                                                                 <  2:76227/238‑1 (MQ=255)                     aCCAAACGCCGCCACCGATCCCCCGTGGCGTTCTACCGGCAGGCCGTTTACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCGGCGAAACGGCGGTCAATTTCGTCCATGCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCgaga                                                                                                                                                                                                                            <  1:444914/251‑1 (MQ=255)                        aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGGCGGGTTCCAGCGGGAATg                                                                                                                                                                                                                         >  1:403346/5‑251 (MQ=255)                        aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATg                                                                                                                                                                                                                         <  2:306315/247‑1 (MQ=255)                                                 cGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCATCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGAttt                                                                                                                                                                                                <  2:260320/251‑1 (MQ=255)                                                      cACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGTTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                                                                                                                                                           <  1:157314/251‑1 (MQ=255)                                                       aCCGGCAGGCCGTTCACCGTCGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTAAGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                                                                                                                                                           <  2:330005/250‑1 (MQ=255)                                                          ggCAGGCCGTTCTCCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGTTTTATCTTCGGCGAAACGGCTGTCAATTTCGTCCAGTCGTTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                                                                                                                                                       <  1:688701/251‑1 (MQ=255)                                                          ggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGCTTTATGGAAAATCTGCGTCACCAGCTCGCAATCATTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTGCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACGCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                                                                                                                                                       <  2:118258/251‑1 (MQ=255)                                                                      aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGAt                                                                                                                                                                           <  1:13574/251‑1 (MQ=255)                                                                             cgcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTGTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCTATAATTa                                                                                                                                                                      <  2:650974/247‑1 (MQ=255)                                                                                   cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTGCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCt                                                                                                                                                              <  1:65577/251‑1 (MQ=255)                                                                                                    aTTTCAGTTTTCTGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGAGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGCTCCCACGGGTTATCTATAGATTTTCCTTCGCCTAATCACTCC‑TGCGCGACGGGTTCCAGCGAGAATGAAGAGAAAATCCCGAAAGCGTATTTCAGCGCCTCGATACCGTCGATAATTATACGCCATTTGGCCTTGGCaaaaa                                                                                                                                              >  2:78215/1‑250 (MQ=255)                                                                                                     tttCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCCATTTTGGCCCTTCGATTCCACGGGTTATCTATAGATTTTTCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGGGTATGAATAGAAATTCCAGAACCCGGATTTCAACACCTCGATACCTCCGCTAATTCGTCGGCCTTTAGACTGGGCaaacaa                                                                                                                                             >  1:269291/1‑247 (MQ=255)                                                                                                            tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGt                                                                                                                                     >  1:702365/5‑251 (MQ=255)                                                                                                            tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGTAAAAAAGACGCCg                                                                                                                                      <  1:555262/246‑1 (MQ=255)                                                                                                                 ggAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGGTAATTTGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGgtttt                                                                                                                                 >  2:466846/1‑250 (MQ=255)                                                                                                                   aaaaTCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTaa                                                                                                                               >  1:434528/4‑250 (MQ=255)                                                                                                                        cggCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCACGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                                                                                                                         <  2:124074/249‑1 (MQ=255)                                                                                                                           cGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCCTTCGATCCCCCGGGTTATCGATAGATTTTCCTTCTCCTAATAACTCC‑TGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCCGCGCCTCGATACCTTCGATAATTAGCCGACCTTTAGCCTGGGCAAACAAGTCGCCGTTTTTAAAGCCGTGCt                                                                                                                      >  2:126111/1‑251 (MQ=255)                                                                                                                             tcaccagccagaaaTCTTTCAGGTTATCAGTAGCCAGAGCCGGTTTATATTCGGCGAAACGGCGGACAACTTAGTCCATTCGATCCCACGGGTTAACCATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGAAGGTTTCCCGCGCGGATGAAGAGAAATTCCAGCACCCGGATTTCCGCGCCCCGATCCCGACGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCAGTTTTTAAAGCCgcgcctg                                                                                                                    >  2:352892/13‑245 (MQ=255)                                                                                                                                               tCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGGTTTCAGCGCCTCGATACCGTCGGTAATTATCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGGGATCGCGCCACTCCACCTcactg                                                                                                  >  2:263346/1‑247 (MQ=255)                                                                                                                                                  ggTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgtt                                                                                                >  1:146901/1‑244 (MQ=255)                                                                                                                                                                 gcgcATGTTTATCTTCGGCGTAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTTTCGATAGATTTTCCTTTGCCTCATTACTCC‑TGAGCGCCGGTTTCCCCCGAGAATTACAAGAAATTCCAGCACGCGGGTTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTATCCTGGGCCAAAAAGACGCCGTTTTTTACGCCGTTCTCTCGCCCCTCCCCCTCCCTGTTTTCGCGccccagcccc                                                                                >  2:52521/1‑244 (MQ=255)                                                                                                                                                                        tttATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGa                                                                          >  1:228785/1‑250 (MQ=255)                                                                                                                                                                             cTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGactacgg                                                                    >  1:498561/1‑245 (MQ=255)                                                                                                                                                                               tCGGAGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACCCC‑TGAGCGTCGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGGCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACg                                                                     <  2:80321/248‑1 (MQ=255)                                                                                                                                                                                       aCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCCAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCTCCCCAGCCGCAACGGAATACGGTAACGCTCgg                                                          >  1:429507/1‑251 (MQ=255)                                                                                                                                                                                            ggTCAATTTCGTCCATTCGCTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCCACGGAATACGGTAACGCTCGGCATGt                                                     >  2:648453/1‑251 (MQ=255)                                                                                                                                                                                               cAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGtttt                                                  <  1:190035/251‑1 (MQ=255)                                                                                                                                                                                               cAATTTCGCCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGtttt                                                  <  2:114403/251‑1 (MQ=255)                                                                                                                                                                                                    cacccgccgctcttccgatctttATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAAGGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                               <  2:207087/228‑1 (MQ=255)                                                                                                                                                                                                          agtctaccccACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCTACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCTGCATGTTTTTGCAGCATTCg                                       <  2:171947/244‑1 (MQ=255)                                                                                                                                                                                                            ccgaacccACGGGTTATCGATAGAGTTTCCTTAGCTAAATAACCCC‑TGAGCGACGGTTTCCAGCGAGAAGGAAGAGAAAGTCCAGAACGCGGATATCAGCGCCTCGATACCGCCGATAATTAGCCGTCTGTTAGCCTGGGCAAAAAAGACGCCGCTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCa                                     <  2:363444/246‑1 (MQ=255)                                                                                                                                                                                                             cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                      >  1:515426/1‑249 (MQ=255)                                                                                                                                                                                                              gATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                      >  2:91577/1‑248 (MQ=255)                                                                                                                                                                                                                     cGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCA‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGCTAATTAGCCGACCTTTAGCCTGGGCACAAAAGACGCCGTTTTTAAAGCCGGTCTCGCGACACTCAAGCTAACTGTTATCGCGCCCCAGCCCCAGAGGAATCCGGGAACGCTCGACATGTTTTTGCAGAATTCGACGATCGAACt                            >  2:167115/1‑251 (MQ=255)                                                                                                                                                                                                                         ttATCGATAGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                               >  1:207087/1‑228 (MQ=255)                                                                                                                                                                                                                              gaacgATTTTCCTTCGCCTAATA‑CTCCCTGCGCGTCGGTTTCCAGCGGGAATGAAGAGAAATTCCAGAACCCGGATTTCAGCGCCCCGATACCGTTGATAATTAGCTGTCCTTTAGCCTGGGAAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACGCCAGCTCACTGTTATTGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCCCCACGt                   <  2:563692/247‑1 (MQ=255)                                                                                                                                                                                                                                 aGATTTTCCTTCGCCTAATAACTCC‑TGAGCGACGGTTCCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTcc                <  2:515426/251‑1 (MQ=255)                                                                                                                                                                                                                                     tttCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAAcc            >  1:309764/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCCTTCGCCTAATAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCg           >  1:45765/1‑250 (MQ=255)                                                                                                                                                                                                                                                 aaTAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcaca  <  1:630101/249‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTAACTCC‑TGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcaca  <  1:126111/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCC‑TGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACA  >  NC_000913/64639‑65127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑